[go: up one dir, main page]

WO2003078577A8 - Clonage des genes du cytochrome p450 a partir de nicotiana - Google Patents

Clonage des genes du cytochrome p450 a partir de nicotiana

Info

Publication number
WO2003078577A8
WO2003078577A8 PCT/US2003/007430 US0307430W WO03078577A8 WO 2003078577 A8 WO2003078577 A8 WO 2003078577A8 US 0307430 W US0307430 W US 0307430W WO 03078577 A8 WO03078577 A8 WO 03078577A8
Authority
WO
WIPO (PCT)
Prior art keywords
nicotiana
cytochrome
cloning
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/007430
Other languages
English (en)
Other versions
WO2003078577A2 (fr
WO2003078577A9 (fr
Inventor
Dongmei Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Smokeless Tobacco Co LLC
Original Assignee
US Smokeless Tobacco Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2003220165A priority Critical patent/AU2003220165A1/en
Application filed by US Smokeless Tobacco Co LLC filed Critical US Smokeless Tobacco Co LLC
Priority to EP03716460A priority patent/EP1572911A4/fr
Priority to CA002477452A priority patent/CA2477452A1/fr
Priority to APAP/P/2004/003122A priority patent/AP2004003122A0/en
Priority to JP2003576572A priority patent/JP2005531297A/ja
Priority to BRPI0308351-9A priority patent/BR0308351A/pt
Priority to KR10-2004-7014250A priority patent/KR20040091124A/ko
Priority to MXPA04008785A priority patent/MXPA04008785A/es
Publication of WO2003078577A2 publication Critical patent/WO2003078577A2/fr
Publication of WO2003078577A9 publication Critical patent/WO2003078577A9/fr
Anticipated expiration legal-status Critical
Publication of WO2003078577A8 publication Critical patent/WO2003078577A8/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
PCT/US2003/007430 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana Ceased WO2003078577A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
MXPA04008785A MXPA04008785A (es) 2002-03-12 2003-03-12 Clonacion de genes p450 de citocromos de nicotiana.
EP03716460A EP1572911A4 (fr) 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana
CA002477452A CA2477452A1 (fr) 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana
APAP/P/2004/003122A AP2004003122A0 (en) 2002-03-12 2003-03-12 Cloning of cytochrome P450 genes from nicotiana
JP2003576572A JP2005531297A (ja) 2002-03-12 2003-03-12 タバコ由来シトクロムｐ４５０遺伝子のクローニング
AU2003220165A AU2003220165A1 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana
KR10-2004-7014250A KR20040091124A (ko) 2002-03-12 2003-03-12 니코티아나 사이토크롬 ｐ450 유전자의 클로닝
BRPI0308351-9A BR0308351A (pt) 2002-03-12 2003-03-12 molécula de ácido nucleico isolada, planta transgênica, e, métodos para produzir uma planta transgênica e para selecionar uma planta contendo uma molécula de ácido nucleico

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36368402P 2002-03-12 2002-03-12
US60/363,684 2002-03-12

Publications (3)

Publication Number Publication Date
WO2003078577A2 WO2003078577A2 (fr) 2003-09-25
WO2003078577A9 WO2003078577A9 (fr) 2004-01-22
WO2003078577A8 true WO2003078577A8 (fr) 2008-01-10

Family

ID=28041798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/007430 Ceased WO2003078577A2 (fr) 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana

Country Status (10)

Country Link
EP (1) EP1572911A4 (fr)
JP (2) JP2005531297A (fr)
KR (1) KR20040091124A (fr)
CN (1) CN101166824A (fr)
AP (1) AP2004003122A0 (fr)
AU (1) AU2003220165A1 (fr)
BR (1) BR0308351A (fr)
CA (1) CA2477452A1 (fr)
MX (1) MXPA04008785A (fr)
WO (1) WO2003078577A2 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
WO2005038018A2 (fr) * 2003-10-16 2005-04-28 U.S. Smokeless Tobacco Company Clonage des genes a p450 du cytochrome de nicotiana
CN1926235B (zh) 2003-10-16 2012-07-18 美国无烟烟草有限责任公司 从烟草克隆细胞色素p450基因
PL1675957T3 (pl) * 2003-10-16 2012-06-29 U S Smokeless Tobacco Company Llc Klonowanie genów cytochromu p450 z Nicotiana
AU2011232795B2 (en) * 2004-04-29 2015-05-28 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
CN103348912B (zh) * 2004-04-29 2016-07-13 美国无烟烟草有限责任公司 烟草属核酸分子及其应用
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
ES2373881T3 (es) * 2004-04-29 2012-02-09 U.S. Smokeless Tobacco Company Llc Clon genómico de nicotina desmetilasa de tabaco y usos del mismo.
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
EP1751278B1 (fr) 2004-04-29 2015-03-18 U.S. Smokeless Tobacco Company LLC Molecules d'acide nucleique de nicotiana et leurs utilisations
JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Ｎｉｃｏｔｉａｎａ核酸分子およびその用途
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
WO2008071673A1 (fr) * 2006-12-13 2008-06-19 Dsm Ip Assets B.V. Procédé de préparation de la pravastatine
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
WO2009064771A2 (fr) 2007-11-12 2009-05-22 North Carolina State University Réduction de la teneur du tabac en alcaloïdes par modification de gènes spécifiques du cytochrome p450
CN102858983B (zh) 2010-01-15 2015-08-12 北卡罗来纳州立大学 最小化烟草中去甲烟碱合成的组合物和方法
RU2013143791A (ru) 2011-02-28 2015-04-10 Алтрия Клаинт Сервисиз Инк. Инбредные растения табака albex1f и albex1ms
JP2014515596A (ja) 2011-02-28 2014-07-03 ノース カロライナ ステイト ユニヴァーシティ タバコ近交系植物ｎｃｂｅｘ１ｆ、ｎｃｂｅｘ１ｍｓ、及びｎｃｅｘ９０
WO2014110363A1 (fr) 2013-01-11 2014-07-17 North Carolina State University Plantes d'autogame de tabac k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src et nc196 src hybride
EP2964016A1 (fr) 2013-03-05 2016-01-13 North Carolina State University Plantes autogames et hybrides de tabac et leurs utilisations
WO2015134438A1 (fr) 2014-03-03 2015-09-11 North Carolina State University Plantes de tabac autogames et hybrides et produits de tabac fabriqués à partir de celles-ci
EP3113602A1 (fr) 2014-03-03 2017-01-11 North Carolina State University Plants de tabac autogames et hybrides et produits de tabac obtenus à partir de ceux-ci
WO2015134427A1 (fr) 2014-03-03 2015-09-11 North Carolina State University Plantes hybrides et autogames de tabac et produits de tabac fabriqués à partir de celles-ci
KR102201082B1 (ko) 2018-10-30 2021-01-08 인하대학교 산학협력단 산소환원반응용 니켈-코발트 산화물의 산소결핍의 유도 방법 및 그 방법에 의한 니켈-코발트 산화물

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE513046T1 (de) * 2001-03-09 2011-07-15 Univ Kentucky Res Found Cytochrom p450s und verwendungen davon
EP2204444A1 (fr) * 2002-10-16 2010-07-07 U.S. Smokeless Tobacco Company Clonage de gènes de cytochrome P450 à partir de Nicotiana

Also Published As

Publication number Publication date
MXPA04008785A (es) 2005-09-30
AP2004003122A0 (en) 2004-09-30
JP2005531297A (ja) 2005-10-20
KR20040091124A (ko) 2004-10-27
CA2477452A1 (fr) 2003-09-25
CN101166824A (zh) 2008-04-23
AU2003220165A8 (en) 2008-03-06
JP2010131027A (ja) 2010-06-17
WO2003078577A2 (fr) 2003-09-25
WO2003078577A9 (fr) 2004-01-22
EP1572911A2 (fr) 2005-09-14
AU2003220165A1 (en) 2003-09-29
BR0308351A (pt) 2007-04-17
EP1572911A4 (fr) 2008-06-25

Similar Documents

Publication Publication Date Title
AU2003301431A1 (en) Cloning of cytochrome p450 genes from nicotiana
AU2003220165A1 (en) Cloning of cytochrome p450 genes from nicotiana
WO2005038018A8 (fr) Clonage des genes a p450 du cytochrome de nicotiana
WO2005038033A8 (fr) Clonage des genes cytochromes p450 a partir de nicotiana
AU2003265978A1 (en) A method of regulating gene expression
AU2003256249A8 (en) Compositions and methods for suppressing eukaryotic gene expression
AU2003297474A1 (en) Methods of inhibiting gene expression by rna interference
AU2002307340A1 (en) Peroxide-driven cytochrome p450 oxygenase variants
AU2003234136A1 (en) Drg11-responsive (dragon) gene family
IL172812A0 (en) Naphthylene derivatives as cytochrome p450 inhibitors
AU2003224049A1 (en) Substituted 2-phenyl-3(2h)-pyridazinones
AU2003206841A1 (en) Genetically engineered phic31-integrase genes
AU2003247948A1 (en) Cytochrome p450 genetic variations
AU2003259185A1 (en) Process for regulating gene expression
AU2003302825A1 (en) Method of design using genetic programming
AU2003258978A1 (en) Recombinant genes for polyketide modifying enzymes
AU2003222965A1 (en) Schizophrenia associated genes
AU2003205062A1 (en) Alteration of restriction endonuclease specificity by genetic selection
HK1091811A (en) Naphthylene derivatives as cytochrome p450 inhibitors
HK1087412A (en) Naphthylene derivatives as cytochrome p450 inhibitors
AU2003206371A1 (en) Simulation of gene expression control using connectrons.
AUPR490101A0 (en) P450 gene regulation ii
AU2003283953A1 (en) Method for gene isolation by cre-trap cloning
AU2003900786A0 (en) Methods of regulating gene expression
AUPS111802A0 (en) Annotation of genome sequences

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
COP Corrected version of pamphlet

Free format text: PAGES 1/31-31/31, DRAWINGS, REPLACED BY NEW PAGES 1/34-34/34; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2477452

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: AP/P/2004/003122

Country of ref document: AP

WWE Wipo information: entry into national phase

Ref document number: 1-2004-501400

Country of ref document: PH

WWE Wipo information: entry into national phase

Ref document number: PA/a/2004/008785

Country of ref document: MX

Ref document number: 1020047014250

Country of ref document: KR

Ref document number: 20038057476

Country of ref document: CN

WWE Wipo information: entry into national phase

Ref document number: 2003576572

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2003716460

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 1200401032

Country of ref document: VN

WWP Wipo information: published in national office

Ref document number: 1020047014250

Country of ref document: KR

WWP Wipo information: published in national office

Ref document number: 2003716460

Country of ref document: EP

ENP Entry into the national phase

Ref document number: PI0308351

Country of ref document: BR

DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)