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WO2003074715A3 - Process for the production of unsaturated fatty acids - Google Patents

Process for the production of unsaturated fatty acids Download PDF

Info

Publication number
WO2003074715A3
WO2003074715A3 PCT/US2003/005788 US0305788W WO03074715A3 WO 2003074715 A3 WO2003074715 A3 WO 2003074715A3 US 0305788 W US0305788 W US 0305788W WO 03074715 A3 WO03074715 A3 WO 03074715A3
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
production
unsaturated fatty
recombinant expression
desaturases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/005788
Other languages
French (fr)
Other versions
WO2003074715A2 (en
Inventor
Andreas Renz
Martijn Gipmans
Ivo Feussner
Claire-Lise Rosenfield
Douglas C Knipple
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Plant Science GmbH
Original Assignee
BASF Plant Science GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF Plant Science GmbH filed Critical BASF Plant Science GmbH
Priority to JP2003573162A priority Critical patent/JP2006503546A/en
Priority to BR0307938-4A priority patent/BR0307938A/en
Priority to CA002477858A priority patent/CA2477858A1/en
Priority to EP03716183A priority patent/EP1527182A4/en
Priority to US10/505,590 priority patent/US7700833B2/en
Priority to AU2003219899A priority patent/AU2003219899A1/en
Publication of WO2003074715A2 publication Critical patent/WO2003074715A2/en
Publication of WO2003074715A3 publication Critical patent/WO2003074715A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)

Abstract

The invention relates to processes for the production of unsaturated fatty acids, preferably of conjugated polyunsaturated fatty acids such as conjugated linoleic acid (CLA), by the recombinant expression of desaturases from insects of the order Lepidoptera. Expression preferably takes place in an organism selected from the group of the plant organisms, yeasts, fungi and algae. Also according to the invention are recombinant expression cassettes for the recombinant expression of desaturases from insects of the order Lepidoptera, and the transgenic organisms transformed with these.
PCT/US2003/005788 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids Ceased WO2003074715A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2003573162A JP2006503546A (en) 2002-03-01 2003-02-27 Process for producing unsaturated fatty acids
BR0307938-4A BR0307938A (en) 2002-03-01 2003-02-27 Process for the production of triglycerides comprising unsaturated fatty acids, triglyceride, use thereof, polypeptide with e-11 desaturase activity, nucleic acid sequence, recombinant expression cassette, recombinant expression vector, and transgenic organism
CA002477858A CA2477858A1 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids
EP03716183A EP1527182A4 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids
US10/505,590 US7700833B2 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids
AU2003219899A AU2003219899A1 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10208812.8 2002-03-01
DE10208812A DE10208812A1 (en) 2002-03-01 2002-03-01 Process for the production of unsaturated fatty acids

Publications (2)

Publication Number Publication Date
WO2003074715A2 WO2003074715A2 (en) 2003-09-12
WO2003074715A3 true WO2003074715A3 (en) 2004-04-29

Family

ID=27740519

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2003/002011 Ceased WO2003074716A2 (en) 2002-03-01 2003-02-27 Methods for the production of unsaturated fatty acids
PCT/US2003/005788 Ceased WO2003074715A2 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/002011 Ceased WO2003074716A2 (en) 2002-03-01 2003-02-27 Methods for the production of unsaturated fatty acids

Country Status (8)

Country Link
EP (1) EP1527182A4 (en)
JP (1) JP2006503546A (en)
CN (1) CN1656226A (en)
AU (2) AU2003208769A1 (en)
BR (1) BR0307938A (en)
CA (1) CA2477858A1 (en)
DE (1) DE10208812A1 (en)
WO (2) WO2003074716A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9359430B2 (en) 2006-11-30 2016-06-07 Abbvie Inc. Abeta conformer selective anti-Abeta globulomer monoclonal antibodies
US9540432B2 (en) 2005-11-30 2017-01-10 AbbVie Deutschland GmbH & Co. KG Anti-Aβ globulomer 7C6 antibodies

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1930277B (en) * 2004-02-27 2011-02-02 巴斯福植物科学有限公司 Method for producing polyunsaturated fatty acids in transgenic plants
DE102005052551A1 (en) * 2005-11-02 2007-05-16 Rothamsted Res Harpenden Process for the preparation of γ-linolenic acid and / or stearidonic acid in transgenic Brassicaceae and Linaceae
SG10201706600VA (en) 2005-11-30 2017-09-28 Abbvie Inc Monoclonal antibodies and uses thereof
MX2009004221A (en) * 2006-10-20 2009-09-10 Univ Arizona State Modified cyanobacteria.
DK2367950T3 (en) * 2008-12-01 2017-07-17 Univ Saarland PREPARATION OF OMEGA-3 FAT ACIDS USING MYXOBACTERIA
JP5168511B2 (en) * 2009-08-25 2013-03-21 独立行政法人農業生物資源研究所 Seed-specific promoter and its use
EP2558494B1 (en) 2010-04-15 2018-05-23 AbbVie Inc. Amyloid-beta binding proteins
US11236351B2 (en) 2010-05-17 2022-02-01 Dow Agrosciences Llc Production of DHA and other LC PUFAs in plants
US9062101B2 (en) 2010-08-14 2015-06-23 AbbVie Deutschland GmbH & Co. KG Amyloid-beta binding proteins
TW201307553A (en) 2011-07-26 2013-02-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
KR101540746B1 (en) * 2011-12-28 2015-08-03 대한민국 Producing method of conjugated linoleic acid
DK3313997T3 (en) 2015-06-26 2020-04-27 Univ Danmarks Tekniske Process for making yeast pheromones in yeast
HUE053613T2 (en) * 2015-11-18 2021-07-28 Provivi Inc Microorganisms for the production of insect pheromones and related compounds
JP2019517797A (en) 2016-06-06 2019-06-27 プロヴィヴィ インコーポレイテッド Semibiosynthetic production of fatty alcohols and fatty aldehydes
WO2018109163A1 (en) 2016-12-16 2018-06-21 Danmarks Tekniske Universitet Methods for producing fatty alcohols and derivatives thereof in yeast
EP4159850A1 (en) 2016-12-16 2023-04-05 Danmarks Tekniske Universitet Production of desaturated fatty alcohols and desaturated fatty alcohol acetates in yeast
US11104921B2 (en) 2017-05-17 2021-08-31 Provivi, Inc. Microorganisms for the production of insect pheromones and related compounds
EP3521439A1 (en) * 2018-01-31 2019-08-07 Commissariat à l'Energie Atomique et aux Energies Alternatives Method for the production of triacylglycerides and fatty acids
CN115103900A (en) * 2019-12-20 2022-09-23 费罗生物公司 Yeast cells and methods for producing E8, E10-dodecadienyl coenzyme A, alexan and derivatives thereof
CN116064266B (en) * 2022-11-01 2024-06-14 四川大学 Recombinant saccharomyces cerevisiae with enhanced salt stress resistance, and construction method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876994A (en) * 1995-11-16 1999-03-02 Cornell Research Foundation, Inc. Pheromone desaturases
US6048838A (en) * 1997-05-05 2000-04-11 Dow Agrosciences Llc Insecticidal protein toxins from xenorhabdus

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2148814T5 (en) * 1995-11-14 2005-12-16 Loders Croklaan B.V. PROCEDURE FOR THE PREPARATION OF MATERIALS WITH A HIGH CONTENT OF CONJUGATED LINOLEIC ACID ISOMERS.
DK0950410T3 (en) * 1998-03-17 2007-05-07 Natural Asa Conjugated linoleic acid compositions
AU4564399A (en) * 1998-06-12 1999-12-30 Abbott Laboratories Polyunsaturated fatty acids in plants
WO2000011012A1 (en) * 1998-08-24 2000-03-02 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
US6706501B1 (en) * 1999-06-30 2004-03-16 Arkion Life Sciences Llc Polynucleotide encoding a propionibacterium linoleate isomerase and uses thereof
EP1190080A1 (en) * 1999-07-06 2002-03-27 BASF Plant Science GmbH PLANTS EXPRESSING $g(D)6-DESATURASE GENES AND OILS FROM THESE PLANTS CONTAINING PUFAS AND METHOD FOR PRODUCING UNSATURATED FATTY ACIDS
WO2001092489A2 (en) * 2000-05-26 2001-12-06 Washington State University Research Foundation Palmitate desaturase gene

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876994A (en) * 1995-11-16 1999-03-02 Cornell Research Foundation, Inc. Pheromone desaturases
US6048838A (en) * 1997-05-05 2000-04-11 Dow Agrosciences Llc Insecticidal protein toxins from xenorhabdus

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540432B2 (en) 2005-11-30 2017-01-10 AbbVie Deutschland GmbH & Co. KG Anti-Aβ globulomer 7C6 antibodies
US9359430B2 (en) 2006-11-30 2016-06-07 Abbvie Inc. Abeta conformer selective anti-Abeta globulomer monoclonal antibodies
US9394360B2 (en) 2006-11-30 2016-07-19 Abbvie Inc. Aβ conformer selective anti-Aβ globulomer monoclonal antibodies

Also Published As

Publication number Publication date
AU2003219899A1 (en) 2003-09-16
WO2003074715A2 (en) 2003-09-12
WO2003074716A3 (en) 2003-12-24
JP2006503546A (en) 2006-02-02
CA2477858A1 (en) 2003-09-12
EP1527182A4 (en) 2005-12-21
EP1527182A2 (en) 2005-05-04
CN1656226A (en) 2005-08-17
AU2003208769A1 (en) 2003-09-16
AU2003208769A8 (en) 2003-09-16
WO2003074716A2 (en) 2003-09-12
DE10208812A1 (en) 2003-09-11
BR0307938A (en) 2005-01-11

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