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WO2003073066A3 - Reagents and methods for assaying bacillus anthracis lethal factor protease - Google Patents

Reagents and methods for assaying bacillus anthracis lethal factor protease Download PDF

Info

Publication number
WO2003073066A3
WO2003073066A3 PCT/US2003/005553 US0305553W WO03073066A3 WO 2003073066 A3 WO2003073066 A3 WO 2003073066A3 US 0305553 W US0305553 W US 0305553W WO 03073066 A3 WO03073066 A3 WO 03073066A3
Authority
WO
WIPO (PCT)
Prior art keywords
lethal factor
bacillus anthracis
assaying
reagents
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/005553
Other languages
French (fr)
Other versions
WO2003073066A2 (en
Inventor
Barry R Cunningham
Richard T Cummings
Jeffrey D Hermes
Scott P Salowe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of WO2003073066A2 publication Critical patent/WO2003073066A2/en
Anticipated expiration legal-status Critical
Publication of WO2003073066A3 publication Critical patent/WO2003073066A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention features Bacillus anthracis lethal factor substrate and assays employing the substrate to measure lethal factor activity and to screen for compounds affecting lethal factor activity (Figure 1). Preferred substrates contain one or more detectable labels and have a sufficiently high turnover rate to be suitable for use in a high throughput screen.
PCT/US2003/005553 2002-02-25 2003-02-21 Reagents and methods for assaying bacillus anthracis lethal factor protease Ceased WO2003073066A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35970702P 2002-02-25 2002-02-25
US60/359,707 2002-02-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/424,954 Continuation US6955891B2 (en) 2002-02-25 2003-04-28 Reagents for assaying Bacillus anthracis lethal factor protease

Publications (2)

Publication Number Publication Date
WO2003073066A2 WO2003073066A2 (en) 2003-09-04
WO2003073066A3 true WO2003073066A3 (en) 2004-12-02

Family

ID=27766128

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/005553 Ceased WO2003073066A2 (en) 2002-02-25 2003-02-21 Reagents and methods for assaying bacillus anthracis lethal factor protease

Country Status (1)

Country Link
WO (1) WO2003073066A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2323527T3 (en) 2002-05-29 2009-07-20 MERCK & CO., INC. USEFUL COMPOUNDS IN THE TREATMENT OF CARBUNK AND INHIBITION OF THE LETAL FACTOR.
CA2566191A1 (en) 2004-05-11 2005-12-15 Merck & Co., Inc. Process for making n-sulfonated-amino acid derivatives
WO2007136436A2 (en) 2006-02-15 2007-11-29 The Government Of The United States Of America, As Represented By The Secretary, Department Of The Health And Human Services, Centers For Disease Control And Prevention Detection of anthrax pathogenicity factors

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001059149A2 (en) * 2000-02-11 2001-08-16 Cellomics, Inc. Peptide biosensors for anthrax protease

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001059149A2 (en) * 2000-02-11 2001-08-16 Cellomics, Inc. Peptide biosensors for anthrax protease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PARK S. ET AL: "Optimized Production And Purification Of Bacillus Anthracis Lethal Factor", PROTEIN EXPRESSION AND PURIFICATION, vol. 18, 2000, pages 293 - 302, XP004435542 *

Also Published As

Publication number Publication date
WO2003073066A2 (en) 2003-09-04

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