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WO2003070977A3 - Procede permettant de detecter des polymorphismes a nucleotide unique - Google Patents

Procede permettant de detecter des polymorphismes a nucleotide unique Download PDF

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Publication number
WO2003070977A3
WO2003070977A3 PCT/EP2003/001420 EP0301420W WO03070977A3 WO 2003070977 A3 WO2003070977 A3 WO 2003070977A3 EP 0301420 W EP0301420 W EP 0301420W WO 03070977 A3 WO03070977 A3 WO 03070977A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequences
stranded
sample
gmeatc
incubating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/001420
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English (en)
Other versions
WO2003070977A2 (fr
Inventor
Andreas Kappel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Recognomics GmbH
Original Assignee
Nanogen Recognomics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanogen Recognomics GmbH filed Critical Nanogen Recognomics GmbH
Priority to AU2003210259A priority Critical patent/AU2003210259A1/en
Publication of WO2003070977A2 publication Critical patent/WO2003070977A2/fr
Anticipated expiration legal-status Critical
Publication of WO2003070977A3 publication Critical patent/WO2003070977A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé pouvant être utilisé pour détecter des mutations en parallèle dans des polymorphismes particuliers à nucléotide unique dans différentes séquences nucléotidiques. Ladite méthode comprend les étapes procédurales suivantes: hybridation de séquences nucléotidiques échantillons à brin unique contenant d(GMeATC) avec des séquences nucléotidiques de référence à brin unique; fixation de séquences nucléotidiques de référence à brin unique ou de séquences nucléotidiques échantillons à brin unique avant ou pendant l'hybridation, ou d'hétéroduplex constitués de séquences nucléotidiques de référence et de séquences nucléotidiques échantillons après ou pendant l'hybridation sur une surface; incubation avec mutS, mutL et mutH afin d'inciser la région complémentaire de la séquence d(GMeATC) dans la séquence d'acides nucléiques de référence, lorsqu'il existe un appariement défectueux dans les séquences d'acides nucléiques de référence et échantillons hybridées; et suppression des extrémités 3' des acides nucléiques de référence incisées et incubation avec une ADN ploymérase et des desoxynucléotidetriphosphates marqués.
PCT/EP2003/001420 2002-02-21 2003-02-13 Procede permettant de detecter des polymorphismes a nucleotide unique Ceased WO2003070977A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003210259A AU2003210259A1 (en) 2002-02-21 2003-02-13 Method for detecting single nucleotide polymorphisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02003888 2002-02-21
EP02003888.1 2002-02-21

Publications (2)

Publication Number Publication Date
WO2003070977A2 WO2003070977A2 (fr) 2003-08-28
WO2003070977A3 true WO2003070977A3 (fr) 2004-09-02

Family

ID=27741104

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001420 Ceased WO2003070977A2 (fr) 2002-02-21 2003-02-13 Procede permettant de detecter des polymorphismes a nucleotide unique

Country Status (2)

Country Link
AU (1) AU2003210259A1 (fr)
WO (1) WO2003070977A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004275865B2 (en) * 2003-09-25 2008-09-25 Third Wave Technologies, Inc. Detection of HPV
FR2920159A1 (fr) * 2007-08-20 2009-02-27 Serial Genetics Sas Soc Par Ac Methode et reactifs pour la quantification, la comparaison et l'identification d'alleles sur support solide
WO2013074632A1 (fr) * 2011-11-14 2013-05-23 Rose Floyd D Purification et identification de nucléotides mésappariés

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993020233A1 (fr) * 1992-03-27 1993-10-14 University Of Maryland At Baltimore Detection de mutations de genes au moyen d'enzymes de reparation d'appariements incorrects
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
WO1995012688A1 (fr) * 1993-01-11 1995-05-11 United States Biochemical Corporation Procedes d'analyse et de manipulation d'adn a l'aide de systemes de reparation d'erreurs d'appariement
WO1996023903A1 (fr) * 1995-01-30 1996-08-08 Ulf Landegren Procede de detection de variations dans des sequences d'acide nucleique
WO1996041002A2 (fr) * 1995-06-07 1996-12-19 Genzyme Corporation Methode d'identification de modification genetique d'adn consistant en un sequencage d'adn et un clonage de position
WO1997021837A1 (fr) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methodes utilisant des systemes de retablissement de mesappariement pour deceler et extraire des sequences mutantes apparaissant au cours d'une amplification enzymatique
WO1999045153A2 (fr) * 1998-03-05 1999-09-10 The University Of Iowa Research Foundation Methode iterative et regenerative de sequencage de l'adn
EP1041160A1 (fr) * 1997-07-31 2000-10-04 The Institute Of Physical & Chemical Research Procede de detection de mutation dans une sequence de bases
DE10038237A1 (de) * 2000-08-04 2002-02-14 Aventis Res & Tech Gmbh & Co Verfahren zum Nachweis von Mutationen in Nucleotidsequenzen

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993020233A1 (fr) * 1992-03-27 1993-10-14 University Of Maryland At Baltimore Detection de mutations de genes au moyen d'enzymes de reparation d'appariements incorrects
US5376526A (en) * 1992-05-06 1994-12-27 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
WO1995012688A1 (fr) * 1993-01-11 1995-05-11 United States Biochemical Corporation Procedes d'analyse et de manipulation d'adn a l'aide de systemes de reparation d'erreurs d'appariement
WO1996023903A1 (fr) * 1995-01-30 1996-08-08 Ulf Landegren Procede de detection de variations dans des sequences d'acide nucleique
WO1996041002A2 (fr) * 1995-06-07 1996-12-19 Genzyme Corporation Methode d'identification de modification genetique d'adn consistant en un sequencage d'adn et un clonage de position
WO1997021837A1 (fr) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methodes utilisant des systemes de retablissement de mesappariement pour deceler et extraire des sequences mutantes apparaissant au cours d'une amplification enzymatique
EP1041160A1 (fr) * 1997-07-31 2000-10-04 The Institute Of Physical & Chemical Research Procede de detection de mutation dans une sequence de bases
WO1999045153A2 (fr) * 1998-03-05 1999-09-10 The University Of Iowa Research Foundation Methode iterative et regenerative de sequencage de l'adn
DE10038237A1 (de) * 2000-08-04 2002-02-14 Aventis Res & Tech Gmbh & Co Verfahren zum Nachweis von Mutationen in Nucleotidsequenzen

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BEAULIEU MARTIN ET AL: "PCR candidate region mismatch scanning: Adaptation to quantitative, high-throughput genotyping", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 29, no. 5, 1 March 2001 (2001-03-01), pages 1114 - 1124, XP002211465, ISSN: 0305-1048 *
GRILLEY M ET AL: "Bidirectional excision in methyl-directed mismatch repair.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 5 JUN 1993, vol. 268, no. 16, 5 June 1993 (1993-06-05), pages 11830 - 11837, XP002255268, ISSN: 0021-9258 *
SMITH J ET AL: "MUTATION DETECTION WITH MUTH, MUTL, AND MUTS MISMATCH REPAIR PROTEINS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 93, 1 April 1996 (1996-04-01), pages 4374 - 4379, XP002030021, ISSN: 0027-8424 *
TAYLOR G R ET AL: "Enzymatic methods for mutation scanning", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING, ELSEVIER SCIENCE PUBLISHING, US, vol. 14, no. 5-6, February 1999 (1999-02-01), pages 181 - 186, XP004158702, ISSN: 1050-3862 *

Also Published As

Publication number Publication date
WO2003070977A2 (fr) 2003-08-28
AU2003210259A1 (en) 2003-09-09

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