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WO2003066882A3 - Method and apparatus for validating dna sequences without sequencing - Google Patents

Method and apparatus for validating dna sequences without sequencing Download PDF

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Publication number
WO2003066882A3
WO2003066882A3 PCT/US2003/003643 US0303643W WO03066882A3 WO 2003066882 A3 WO2003066882 A3 WO 2003066882A3 US 0303643 W US0303643 W US 0303643W WO 03066882 A3 WO03066882 A3 WO 03066882A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
dna sequences
methods
nucleic acid
molecular masses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/003643
Other languages
French (fr)
Other versions
WO2003066882A2 (en
Inventor
Gregory T Went
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tethys Bioscience Inc
Original Assignee
Tethys Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tethys Bioscience Inc filed Critical Tethys Bioscience Inc
Priority to AU2003215083A priority Critical patent/AU2003215083A1/en
Publication of WO2003066882A2 publication Critical patent/WO2003066882A2/en
Publication of WO2003066882A3 publication Critical patent/WO2003066882A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a system comprising methods by which the sequence of a biologically or non-biologically derived nucleic acid can be determined without sequencing. The methods preferably compare the molecular masses of subsequences generated from the target sequence with predicted molecular masses by a database look-up step. Computer-implemented methods are provided to analyse the experimental results and to determine any sub-regions of the nucleic acid containing one or more variations.
PCT/US2003/003643 2002-02-06 2003-02-06 Method and apparatus for validating dna sequences without sequencing Ceased WO2003066882A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003215083A AU2003215083A1 (en) 2002-02-06 2003-02-06 Method and apparatus for validating dna sequences without sequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35464002P 2002-02-06 2002-02-06
US60/354,640 2002-02-06

Publications (2)

Publication Number Publication Date
WO2003066882A2 WO2003066882A2 (en) 2003-08-14
WO2003066882A3 true WO2003066882A3 (en) 2004-01-22

Family

ID=27734402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/003643 Ceased WO2003066882A2 (en) 2002-02-06 2003-02-06 Method and apparatus for validating dna sequences without sequencing

Country Status (2)

Country Link
AU (1) AU2003215083A1 (en)
WO (1) WO2003066882A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005100607A1 (en) 2004-04-09 2005-10-27 The Trustees Of Boston University Method for de novo detection of sequences in nucleic acids:target sequencing by fragmentation
EP2145180B1 (en) * 2007-04-13 2013-12-04 Sequenom, Inc. Comparative sequence analysis processes and systems
CN113658635B (en) * 2021-08-24 2023-09-29 北京诺禾致源科技股份有限公司 Automatic determination method and device for nucleic acid detection result and application of automatic determination method and device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US6277573B1 (en) * 1995-03-17 2001-08-21 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US6303309B1 (en) * 1996-05-13 2001-10-16 Sequenom, Inc. Method for dissociating biotin complexes
US6566055B1 (en) * 1996-09-19 2003-05-20 Sequenom, Inc. Methods of preparing nucleic acids for mass spectrometric analysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6277573B1 (en) * 1995-03-17 2001-08-21 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US6303309B1 (en) * 1996-05-13 2001-10-16 Sequenom, Inc. Method for dissociating biotin complexes
US6566055B1 (en) * 1996-09-19 2003-05-20 Sequenom, Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation

Also Published As

Publication number Publication date
AU2003215083A1 (en) 2003-09-02
WO2003066882A2 (en) 2003-08-14
AU2003215083A8 (en) 2003-09-02

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