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WO2003046512A3 - Procede permettant l'amplification de signaux de titrage biologique moleculaires - Google Patents

Procede permettant l'amplification de signaux de titrage biologique moleculaires Download PDF

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Publication number
WO2003046512A3
WO2003046512A3 PCT/US2002/038104 US0238104W WO03046512A3 WO 2003046512 A3 WO2003046512 A3 WO 2003046512A3 US 0238104 W US0238104 W US 0238104W WO 03046512 A3 WO03046512 A3 WO 03046512A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
bioassays
molecules
assay signals
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/038104
Other languages
English (en)
Other versions
WO2003046512A2 (fr
Inventor
Fengchun Ye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Royce Technologies LLC USA
Original Assignee
Royce Technologies LLC USA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002363703A external-priority patent/CA2363703A1/fr
Application filed by Royce Technologies LLC USA filed Critical Royce Technologies LLC USA
Priority to AU2002363953A priority Critical patent/AU2002363953A1/en
Publication of WO2003046512A2 publication Critical patent/WO2003046512A2/fr
Publication of WO2003046512A3 publication Critical patent/WO2003046512A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention a trait à des réactifs et à un procédé permettant une amplification in vitro efficace de signaux de titrage biologique. Ledit procédé fait appel à une paire d'amorces oligonucléotidiques complémentaires « bout à bout », pour former en continu une molécule hybride double brin et hautement répétitive. Etant donné qu'elle est liée par covalence et ajoutée à la molécule de sondage dans les titrages biologiques, et est également marquée de manière compatible, cette molécule hybride peut amplifier les signaux de détection des centaines de fois en un court laps de temps. Ce procédé peut avoir des applications très larges dans des titrages biologiques faisant intervenir l'hybridation moléculaire, la détection immunochimique, et d'autres interactions particulières entre deux molécules ou entre des molécules et des cellules.
PCT/US2002/038104 2001-11-23 2002-11-25 Procede permettant l'amplification de signaux de titrage biologique moleculaires Ceased WO2003046512A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002363953A AU2002363953A1 (en) 2001-11-23 2002-11-25 Method for amplification of molecular bio-assay signals

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA002363703A CA2363703A1 (fr) 2001-11-23 2001-11-23 Methode d'amplification des signaux moleculaires pour les epreuves biologiques
CA2,363,703 2001-11-23
US10/299,066 2002-11-18
US10/299,066 US20030124604A1 (en) 2001-11-23 2002-11-18 Method for amplification of molecular bio-assay signals

Publications (2)

Publication Number Publication Date
WO2003046512A2 WO2003046512A2 (fr) 2003-06-05
WO2003046512A3 true WO2003046512A3 (fr) 2004-02-05

Family

ID=25682800

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/038104 Ceased WO2003046512A2 (fr) 2001-11-23 2002-11-25 Procede permettant l'amplification de signaux de titrage biologique moleculaires

Country Status (2)

Country Link
AU (1) AU2002363953A1 (fr)
WO (1) WO2003046512A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009026651A1 (fr) * 2007-08-30 2009-03-05 Commonwealth Scientific And Industrial Research Organisation Méthode de détection d'un acide nucléique

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LIZARDI ET AL.: "Mutation detection and single-molecul counting using isothermal rolling-circle amplification", NATURE GENETICS, vol. 19, July 1998 (1998-07-01), pages 225 - 232, XP002922815 *
SCHWEITZER ET AL.: "Immunoassays with rolling circle DNA amplification: A versatile platform for ultra sensitive antigen detection", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 18, 29 August 2000 (2000-08-29), pages 10113 - 10119, XP002207138 *

Also Published As

Publication number Publication date
WO2003046512A2 (fr) 2003-06-05
AU2002363953A1 (en) 2003-06-10
AU2002363953A8 (en) 2003-06-10

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