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WO2003044168A3 - Facilitation de l'interference d'arn - Google Patents

Facilitation de l'interference d'arn Download PDF

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Publication number
WO2003044168A3
WO2003044168A3 PCT/US2002/036725 US0236725W WO03044168A3 WO 2003044168 A3 WO2003044168 A3 WO 2003044168A3 US 0236725 W US0236725 W US 0236725W WO 03044168 A3 WO03044168 A3 WO 03044168A3
Authority
WO
WIPO (PCT)
Prior art keywords
facilitation
rna interference
rnai
invertebrates
elegans
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/036725
Other languages
English (en)
Other versions
WO2003044168A9 (fr
WO2003044168A2 (fr
Inventor
Craig C Mello
Darryl Conte Jr
Chun-Chieh Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Massachusetts Amherst
Original Assignee
University of Massachusetts Amherst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Massachusetts Amherst filed Critical University of Massachusetts Amherst
Priority to AU2002360394A priority Critical patent/AU2002360394A1/en
Publication of WO2003044168A2 publication Critical patent/WO2003044168A2/fr
Publication of WO2003044168A9 publication Critical patent/WO2003044168A9/fr
Anticipated expiration legal-status Critical
Publication of WO2003044168A3 publication Critical patent/WO2003044168A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07048—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne des compositions et procédés destinés à induire ou accentuer l'ARNi dans les cellules, systèmes et organismes au moyen de molécules responsables de la médiation de l'ARNi chez les invertébrés, par exemple chez C. elegans.
PCT/US2002/036725 2001-11-16 2002-11-15 Facilitation de l'interference d'arn Ceased WO2003044168A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002360394A AU2002360394A1 (en) 2001-11-16 2002-11-15 Facilitation of rna interference

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33381101P 2001-11-16 2001-11-16
US60/333,811 2001-11-16
US33167201P 2001-11-19 2001-11-19
US60/331,672 2001-11-19

Publications (3)

Publication Number Publication Date
WO2003044168A2 WO2003044168A2 (fr) 2003-05-30
WO2003044168A9 WO2003044168A9 (fr) 2004-05-06
WO2003044168A3 true WO2003044168A3 (fr) 2004-08-26

Family

ID=26987858

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/036725 Ceased WO2003044168A2 (fr) 2001-11-16 2002-11-15 Facilitation de l'interference d'arn

Country Status (3)

Country Link
US (1) US20030114409A1 (fr)
AU (1) AU2002360394A1 (fr)
WO (1) WO2003044168A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
ITRM20040196A1 (it) * 2004-04-19 2004-07-19 Univ Roma Metodo per ottenere un silenziamento genico stabile in cellule eucariote.
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
EA015584B1 (ru) 2005-03-23 2011-10-31 Генмаб А/С Антитело к cd38 человека и его применение
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2142651B1 (fr) 2007-04-27 2013-05-22 Pfenex Inc. Procédé pour rapidement cribler des hôtes microbiens et identifier certaines souches ayant un rendement et/ou une qualité d'expression des protéines hétérologues améliorés
US8765370B2 (en) * 2009-06-11 2014-07-01 Scinopharm Taiwan, Ltd Inhibition-based high-throughput screen strategy for cell clones
TWI475109B (zh) * 2010-06-03 2015-03-01 Scinopharm Taiwan Ltd 通過抑制報告基因表現量的手段進行高產量細胞株選殖之策略

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6218142B1 (en) * 1997-03-05 2001-04-17 Michael Wassenegger Nucleic acid molecules encoding polypeptides having the enzymatic activity of an RNA-directed RNA polymerase (RDRP)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981247A (en) * 1995-09-27 1999-11-09 Emory University Recombinant hepatitis C virus RNA replicase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6218142B1 (en) * 1997-03-05 2001-04-17 Michael Wassenegger Nucleic acid molecules encoding polypeptides having the enzymatic activity of an RNA-directed RNA polymerase (RDRP)

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
AHLQUIST P.: "RNA-Dependent RNA Polymerases, Viruses, and RNA Silencing", SCIENCE, vol. 296, no. 5571, 17 May 2002 (2002-05-17), pages 1270 - 1273, XP002978511 *
CHIU Y-L. ET AL: "RNAi in Human Cells: Basic Structural and Functional Features of Small Interfering RNA", MOLECULAR CELL, vol. 10, September 2002 (2002-09-01), pages 549 - 561, XP002978510 *
DATABASE GENBANK [online] 16 November 2000 (2000-11-16), MAINE E.M.: "Caenorhabditis elegans RRF-1 (rrf-1) mRNA, complete cds", XP002978507, Database accession no. AF159144 *
DATABASE GENBANK [online] 16 November 2000 (2000-11-16), MAINE ET AL: "Caenorhabditis elegans RNA-directed RNA polymerase related EGO-1 (ego-1) mRNA, complete cds", XP002978506, Database accession no. AF159143 *
DE WIT ET AL: "The tomato RNA-directed RNA polymerase has no effect on gene silencing by RNA interference in transgenic mice", TRANSGENIC RESEARCH, vol. 11, no. 3, June 2002 (2002-06-01), pages 305 - 310, XP002978591 *
SCHWARZ ET AL: "Evidence that siRNAs Function as Guides, Not Primers, in the Drosophila and Human RNAi Pathways", MOLECULAR CELL, vol. 10, September 2002 (2002-09-01), pages 537 - 548, XP002978509 *
SIJEN ET AL: "On the Role of RNA Amplification in dsRNA-Triggered Gene Silencing", CELL, vol. 107, 16 November 2001 (2001-11-16), pages 465 - 476, XP002978508 *
SMARDON ET AL: "EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans", CURRENT BIOLOGY, vol. 10, no. 4, February 2000 (2000-02-01), pages 169 - 178, XP002978505 *
XIE ET AL: "An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense", PNAS, vol. 98, no. 11, 22 May 2001 (2001-05-22), pages 6516 - 6521, XP002978504 *

Also Published As

Publication number Publication date
US20030114409A1 (en) 2003-06-19
AU2002360394A8 (en) 2003-06-10
AU2002360394A1 (en) 2003-06-10
WO2003044168A9 (fr) 2004-05-06
WO2003044168A2 (fr) 2003-05-30

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