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WO2003040373A3 - Production of l-lysine by genetically modified corynebacterium glutamicum strains - Google Patents

Production of l-lysine by genetically modified corynebacterium glutamicum strains Download PDF

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Publication number
WO2003040373A3
WO2003040373A3 PCT/EP2002/008464 EP0208464W WO03040373A3 WO 2003040373 A3 WO2003040373 A3 WO 2003040373A3 EP 0208464 W EP0208464 W EP 0208464W WO 03040373 A3 WO03040373 A3 WO 03040373A3
Authority
WO
WIPO (PCT)
Prior art keywords
lysine
production
genetically modified
corynebacterium glutamicum
glutamicum strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/008464
Other languages
French (fr)
Other versions
WO2003040373A2 (en
Inventor
Brigitte Bathe
Caroline Kreutzer
Bettina Moeckel
Georg Thierbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
Original Assignee
Degussa GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa GmbH filed Critical Degussa GmbH
Priority to BR0211723-1A priority Critical patent/BR0211723A/en
Priority to CA002455878A priority patent/CA2455878A1/en
Priority to AU2002325923A priority patent/AU2002325923A1/en
Priority to EP02760293A priority patent/EP1414970A2/en
Priority to US10/358,405 priority patent/US7160711B2/en
Publication of WO2003040373A2 publication Critical patent/WO2003040373A2/en
Publication of WO2003040373A3 publication Critical patent/WO2003040373A3/en
Anticipated expiration legal-status Critical
Priority to US11/612,208 priority patent/US20070111291A1/en
Priority to US12/565,533 priority patent/US20100159523A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.
PCT/EP2002/008464 2001-08-06 2002-07-30 Production of l-lysine by genetically modified corynebacterium glutamicum strains Ceased WO2003040373A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
BR0211723-1A BR0211723A (en) 2001-08-06 2002-07-30 Corineform bacteria that produce chemical compounds i
CA002455878A CA2455878A1 (en) 2001-08-06 2002-07-30 Production of l-lysine by genetically modified corynebacterium glutamicum strains
AU2002325923A AU2002325923A1 (en) 2001-08-06 2002-07-30 Production of l-lysine by genetically modified corynebacterium glutamicum strains
EP02760293A EP1414970A2 (en) 2001-08-06 2002-07-30 Production of l-lysine by genetically modified corynebacterium glutamicum strains
US10/358,405 US7160711B2 (en) 2001-08-06 2003-02-05 Coryneform bacteria which produce chemical compounds I
US11/612,208 US20070111291A1 (en) 2001-08-06 2006-12-18 Coryneform Bacteria Which Produce Chemical Compounds I
US12/565,533 US20100159523A1 (en) 2001-08-06 2009-09-23 Coryneform Bacteria Which Produce Chemical Compounds I

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30987801P 2001-08-06 2001-08-06
US60/309,878 2001-08-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/358,405 Continuation-In-Part US7160711B2 (en) 2001-08-06 2003-02-05 Coryneform bacteria which produce chemical compounds I

Publications (2)

Publication Number Publication Date
WO2003040373A2 WO2003040373A2 (en) 2003-05-15
WO2003040373A3 true WO2003040373A3 (en) 2003-12-18

Family

ID=23200067

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/008464 Ceased WO2003040373A2 (en) 2001-08-06 2002-07-30 Production of l-lysine by genetically modified corynebacterium glutamicum strains

Country Status (6)

Country Link
EP (1) EP1414970A2 (en)
CN (1) CN100554426C (en)
AU (1) AU2002325923A1 (en)
BR (1) BR0211723A (en)
CA (1) CA2455878A1 (en)
WO (1) WO2003040373A2 (en)

Families Citing this family (52)

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US7160711B2 (en) 2001-08-06 2007-01-09 Degussa Ag Coryneform bacteria which produce chemical compounds I
US6844176B1 (en) 2001-10-16 2005-01-18 Degussa Ag Alleles of the lysC gene from corynebacteria
DE10359594A1 (en) 2003-12-18 2005-07-28 Basf Ag PEF TU-expression units
DE102004035067A1 (en) * 2004-07-20 2006-02-16 Basf Ag P1-35 expression units
DE102005013676A1 (en) 2005-03-24 2006-09-28 Degussa Ag Alleles of the zwf gene from coryneform bacteria
DE102005023829A1 (en) 2005-05-24 2006-11-30 Degussa Ag Alleles of the opcA gene from coryneform bacteria
DE102005047596A1 (en) 2005-10-05 2007-04-12 Degussa Ag Process for the fermentative production of L-amino acids using coryneform bacteria
DE102006026328A1 (en) * 2006-06-02 2008-01-03 Evonik Degussa Gmbh Process for the preparation of a feed additive containing L-lysine
KR100830289B1 (en) * 2007-01-18 2008-05-16 씨제이제일제당 (주) L-arginine production mutant strain and preparation method thereof
DE102007005072A1 (en) 2007-02-01 2008-08-07 Evonik Degussa Gmbh Process for the fermentative production of cadaverine
JP2011067095A (en) 2008-01-10 2011-04-07 Ajinomoto Co Inc Method for producing target substance by fermentation process
KR100987281B1 (en) * 2008-01-31 2010-10-12 씨제이제일제당 (주) Improved promoter and method for producing L-lysine using the same
DE102008001874A1 (en) 2008-05-20 2009-11-26 Evonik Degussa Gmbh Process for the preparation of L-amino acids
DE102009030342A1 (en) 2009-06-25 2010-12-30 Evonik Degussa Gmbh Process for the fermentative production of organic chemical compounds
DE102010003419B4 (en) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Process for the fermentative production of L-ornithine
DE102010025124A1 (en) 2010-06-25 2011-12-29 Forschungszentrum Jülich GmbH Process for the preparation of D-amino acids, microorganism, and vector
EP2479279A1 (en) 2011-01-20 2012-07-25 Evonik Degussa GmbH Method for producing sulphuric amino acids by means of fermentation
WO2012114256A1 (en) 2011-02-22 2012-08-30 Basf Se Processes and recombinant microorganisms for the production of cadaverine
DE102011006716A1 (en) 2011-04-04 2012-10-04 Evonik Degussa Gmbh Microorganism and process for the fermentative production of an organic chemical compound
JP2014131487A (en) 2011-04-18 2014-07-17 Ajinomoto Co Inc Method for producing l-cysteine
DE102011118019A1 (en) 2011-06-28 2013-01-03 Evonik Degussa Gmbh Variants of the promoter of the glyceraldehyde-3-phosphate dehydrogenase-encoding gap gene
WO2013065772A1 (en) 2011-11-02 2013-05-10 味の素株式会社 Method for secreting and producing proteins
EP2778228B1 (en) 2011-11-11 2017-05-17 Ajinomoto Co., Inc. Method for producing 2-ketoglutaric acid and derivatives thereof by using a bacterium from the genus pantoea or the genus corynebacterium
EP2628792A1 (en) 2012-02-17 2013-08-21 Evonik Industries AG Cell with reduced ppGppase activity
EP2631298A1 (en) 2012-02-22 2013-08-28 Evonik Industries AG Biotechnological method for producing butanol and butyric acid
PL2841568T3 (en) 2012-04-27 2018-07-31 Evonik Technochemie Gmbh Feedback-resistant alpha-isopropylmalate synthases
EP2674489A1 (en) 2012-06-15 2013-12-18 Evonik Industries AG Biotechnological 2-hydroxyisobutyric acid production
ES2689754T3 (en) 2012-08-20 2018-11-15 Evonik Degussa Gmbh Procedure for the fermentative preparation of L-amino acids using improved strains of the Enterobacteriaceae family
DE102012016716A1 (en) 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH A process for the preparation of vectors comprising a gene coding for an enzyme which has been reduced or eliminated in its feedback inhibition and the use thereof for the production of amino acids and nucleotides
EP2762571A1 (en) 2013-01-30 2014-08-06 Evonik Industries AG Microorganism and method for the production of amino acids by fermentation
EP2811028B1 (en) 2013-06-03 2017-02-01 Evonik Degussa GmbH Process for producing L-valine employing recombinant Corynebacteria comprising the propionate-inducible ilvBN operon
CN103374540B (en) * 2013-08-13 2015-04-22 廊坊梅花生物技术开发有限公司 Corynebacterium glutamicum and application thereof
DE102014208199A1 (en) 2014-04-30 2015-11-05 Evonik Degussa Gmbh Process for the production of L-amino acids using an alkaliphilic bacterium
PL2940143T3 (en) 2014-04-30 2020-06-29 Evonik Operations Gmbh Method for the production of l-amino acids using an alkaliphilic bacterium
EP2940039A1 (en) 2014-04-30 2015-11-04 Evonik Degussa GmbH Method for the production of l-amino acids in coryne bacteria using a glycine splitting system
EP2940144A1 (en) 2014-04-30 2015-11-04 Evonik Degussa GmbH Method for the production of l-lysine using an alkaliphilic bacterium
BR112018011503A2 (en) 2015-12-07 2018-12-04 Zymergen Inc corynebacterium glutamicum promoters
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
KR101756338B1 (en) * 2016-01-15 2017-07-10 고려대학교 산학협력단 Variant Microorganism for Producing L-Cystein and Method for Preparing L-Cystein Using thereof
CN105838740B (en) * 2016-05-03 2020-11-06 湖北大学 Method for preparing nano red element selenium by using tea tree endophytic asplenium
KR102345899B1 (en) 2016-06-30 2021-12-31 지머젠 인코포레이티드 Methods for generating bacterial hemoglobin libraries and uses thereof
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. METHODS OF PRODUCING A GLUCOSE PERMEASE BANK AND USES THEREOF
WO2018007560A1 (en) * 2016-07-08 2018-01-11 Metabolic Explorer Method for the fermentative production of molecules of interest by microorganisms comprising genes coding sugar phosphotransferase system (pts)
CN106635944A (en) * 2016-12-29 2017-05-10 廊坊梅花生物技术开发有限公司 Glutamic acid corynebacterium and construction method and application thereof
EP3635117A2 (en) 2017-06-07 2020-04-15 Zymergen, Inc. Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
EP3415622A1 (en) 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
KR102011994B1 (en) * 2017-06-30 2019-08-20 씨제이제일제당 주식회사 Novel aspartokinase variant and method of producing L-amino acid using thereof
CN109536428B (en) * 2018-12-07 2022-08-30 武汉远大弘元股份有限公司 Genetically engineered bacterium for producing L-isoleucine and construction method and application thereof
CN111471638B (en) * 2020-05-22 2021-11-23 江南大学 Construction and application of corynebacterium glutamicum mutant strain capable of producing L-homoserine
EP4105332A4 (en) * 2021-05-07 2022-12-28 CJ Cheiljedang Corporation NEW PROMOTER AND ITS USE
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Also Published As

Publication number Publication date
AU2002325923A1 (en) 2003-05-19
WO2003040373A2 (en) 2003-05-15
EP1414970A2 (en) 2004-05-06
CA2455878A1 (en) 2003-05-15
CN1539015A (en) 2004-10-20
CN100554426C (en) 2009-10-28
BR0211723A (en) 2004-09-21

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