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WO2002101358A3 - Multiplexed detection methods - Google Patents

Multiplexed detection methods Download PDF

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Publication number
WO2002101358A3
WO2002101358A3 PCT/US2002/018634 US0218634W WO02101358A3 WO 2002101358 A3 WO2002101358 A3 WO 2002101358A3 US 0218634 W US0218634 W US 0218634W WO 02101358 A3 WO02101358 A3 WO 02101358A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
probes
multiplexed detection
method includes
target molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/018634
Other languages
French (fr)
Other versions
WO2002101358A9 (en
WO2002101358A2 (en
Inventor
Jian-Bing Fan
John R Stuelpnagel
Mark S Chee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/915,231 external-priority patent/US6890741B2/en
Application filed by Illumina Inc filed Critical Illumina Inc
Priority to EP02744306A priority Critical patent/EP1395805A4/en
Priority to CA002450139A priority patent/CA2450139A1/en
Publication of WO2002101358A2 publication Critical patent/WO2002101358A2/en
Publication of WO2002101358A9 publication Critical patent/WO2002101358A9/en
Publication of WO2002101358A3 publication Critical patent/WO2002101358A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention permits highly multiplexed detection of target molecules in a sample. The method includes contacting target analytes with a composition comprising an amplification enzyme and first and second target probes. The first and second target probes comprise first and second bioactive agent respectively, that specifically bind to the first and second target molecules. The probes may also comprise first and second adapter sequences, respectively, such and that the first adapter sequence identifies the first target molecule and the second adapter sequence identifies the second target molecule, and first and section upstream universal priming sequences, respectively. Finally, following amplification the method includes detecting the first and second amplicons, whereby the first and second target molecules, respectively are detected.
PCT/US2002/018634 2001-06-11 2002-06-11 Multiplexed detection methods Ceased WO2002101358A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02744306A EP1395805A4 (en) 2001-06-11 2002-06-11 Multiplexed detection methods
CA002450139A CA2450139A1 (en) 2001-06-11 2002-06-11 Multiplexed detection methods

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US29760901P 2001-06-11 2001-06-11
US60/297,609 2001-06-11
US09/915,231 US6890741B2 (en) 2000-02-07 2001-07-24 Multiplexed detection of analytes
US09/915,231 2001-07-24
US31127101P 2001-08-09 2001-08-09
US60/311,271 2001-08-09

Publications (3)

Publication Number Publication Date
WO2002101358A2 WO2002101358A2 (en) 2002-12-19
WO2002101358A9 WO2002101358A9 (en) 2003-03-27
WO2002101358A3 true WO2002101358A3 (en) 2003-12-11

Family

ID=27404500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/018634 Ceased WO2002101358A2 (en) 2001-06-11 2002-06-11 Multiplexed detection methods

Country Status (3)

Country Link
EP (1) EP1395805A4 (en)
CA (1) CA2450139A1 (en)
WO (1) WO2002101358A2 (en)

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WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
US7241629B2 (en) 2001-12-20 2007-07-10 Corning Incorporated Detectable labels, methods of manufacture and use
EP1660674B1 (en) * 2003-09-10 2010-03-17 Althea Technologies, Inc. Expression profiling using microarrays
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
AU2013226081B2 (en) 2012-02-27 2018-06-14 Becton, Dickinson And Company Compositions and kits for molecular counting
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3472359B1 (en) 2016-06-21 2022-03-16 10X Genomics, Inc. Nucleic acid sequencing
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
KR102790050B1 (en) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 Sample indexing for single cells
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
ES3014208T3 (en) 2018-05-03 2025-04-21 Becton Dickinson Co Molecular barcoding on opposite transcript ends
US20220042090A1 (en) * 2018-09-14 2022-02-10 Cold Spring Harbor Laboratory PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL)
CN118853827A (en) 2018-10-01 2024-10-29 贝克顿迪金森公司 Determine 5' transcript sequence
CN112673098B (en) 2018-10-31 2023-01-06 亿明达股份有限公司 Polymerases, compositions and methods of use
JP7618548B2 (en) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー Whole-transcriptome analysis of single cells using random priming
WO2020117968A2 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
CN114051534B (en) 2019-07-22 2025-02-21 贝克顿迪金森公司 Single-cell chromatin immunoprecipitation sequencing assay
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
EP4090763B1 (en) 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
EP4471155A3 (en) 2020-01-29 2024-12-18 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use

Citations (2)

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US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays

Family Cites Families (6)

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RO113496B1 (en) * 1990-05-04 1998-07-30 Chiron Corp Emeryville Polypeptide / poynucleotide hybride molecular probe and method for amplifying an immunologic determination detectable signal
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
AT407160B (en) * 1998-06-04 2001-01-25 Immuno Ag METHOD FOR DETERMINING ANTIGENS
WO2000058516A2 (en) * 1999-03-26 2000-10-05 Whitehead Institute For Biomedical Research Universal arrays
ATE413467T1 (en) * 1999-04-20 2008-11-15 Illumina Inc DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1395805A4 *

Also Published As

Publication number Publication date
WO2002101358A9 (en) 2003-03-27
WO2002101358A2 (en) 2002-12-19
EP1395805A4 (en) 2005-03-09
CA2450139A1 (en) 2002-12-19
EP1395805A2 (en) 2004-03-10

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