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WO2002101004A3 - Extension cyclique a basse temperature d'adn a haute specificite d'amorcage - Google Patents

Extension cyclique a basse temperature d'adn a haute specificite d'amorcage Download PDF

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Publication number
WO2002101004A3
WO2002101004A3 PCT/IB2002/003341 IB0203341W WO02101004A3 WO 2002101004 A3 WO2002101004 A3 WO 2002101004A3 IB 0203341 W IB0203341 W IB 0203341W WO 02101004 A3 WO02101004 A3 WO 02101004A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
low
temperature
temperature cycle
cycle extension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2002/003341
Other languages
English (en)
Other versions
WO2002101004A2 (fr
Inventor
Guo Fan Hong
Yongjie Yang
Jia Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Mendel DNA Center Co Ltd
Original Assignee
Shanghai Mendel DNA Center Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/878,131 external-priority patent/US20030087237A1/en
Application filed by Shanghai Mendel DNA Center Co Ltd filed Critical Shanghai Mendel DNA Center Co Ltd
Priority to CA002449560A priority Critical patent/CA2449560A1/fr
Priority to JP2003503756A priority patent/JP2005514003A/ja
Priority to EP02758704A priority patent/EP1436391A4/fr
Publication of WO2002101004A2 publication Critical patent/WO2002101004A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002101004A3 publication Critical patent/WO2002101004A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/113—Cycle sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes d'extension d'une amorce ou d'une paire d'amorces dans l'amplification d'ADN cyclique à basse température de séquençage de cycle et PCR. Ces méthodes consistent en particulier à utiliser de façon combinée des ADN polymérases modérément thermostables en présence d'une faible concentration de glycérol ou d'éthylène glycol, ou de mélanges de ceux-ci, comme agents destinés à réduire la température de fusion de l'ADN (c'est-à-dire la température à laquelle les deux brins d'ADN sont dénaturés). L'invention concerne également des mélanges de réaction prédistribués d'ADN polymérase haute fidélité à grande capacité de traitement, stables à température ambiante pendant plusieurs semaines dans des kits prêts à l'emploi.
PCT/IB2002/003341 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage Ceased WO2002101004A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002449560A CA2449560A1 (fr) 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage
JP2003503756A JP2005514003A (ja) 2001-06-08 2002-06-05 高いプライミング特異性を有するｄｎａの低温サイクル伸長
EP02758704A EP1436391A4 (fr) 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/878,131 2001-06-08
US09/878,131 US20030087237A1 (en) 2001-04-30 2001-06-08 Low-temperature cycle extension of DNA with high polymerization specificity

Publications (2)

Publication Number Publication Date
WO2002101004A2 WO2002101004A2 (fr) 2002-12-19
WO2002101004A3 true WO2002101004A3 (fr) 2004-04-29

Family

ID=25371448

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/003341 Ceased WO2002101004A2 (fr) 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage

Country Status (5)

Country Link
EP (1) EP1436391A4 (fr)
JP (1) JP2005514003A (fr)
CA (1) CA2449560A1 (fr)
RU (1) RU2004100107A (fr)
WO (1) WO2002101004A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023601B2 (en) 2002-09-12 2015-05-05 Gen9, Inc. Microarray synthesis and assembly of gene-length polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7632642B2 (en) * 2004-10-18 2009-12-15 Brandeis University Primers, probes and methods for nucleic acid amplification
WO2007123742A2 (fr) * 2006-03-31 2007-11-01 Codon Devices, Inc. Méthodes et compositions améliorant la fidélité d'assemblage de plusieurs acides nucléiques
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
JP5707712B2 (ja) * 2009-03-27 2015-04-30 東洋紡株式会社 核酸増幅のための新規な核酸増幅用組成物
WO2011056872A2 (fr) 2009-11-03 2011-05-12 Gen9, Inc. Procédés et dispositifs microfluidiques pour la manipulation de gouttelettes dans un ensemble polynucléotidique haute fidélité
EP2637780B1 (fr) 2010-11-12 2022-02-09 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
EP2944693B1 (fr) 2011-08-26 2019-04-24 Gen9, Inc. Compositions et procédés pour ensemble haute fidélité d'acides nucléiques
WO2013033528A1 (fr) 2011-09-01 2013-03-07 Jennifer Ong Compositions et procédés associés à des variants d'adn polymérases et d'adn polymérases synthétiques
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN111876409A (zh) 2012-04-24 2020-11-03 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
JP6402905B2 (ja) * 2014-08-08 2018-10-10 東洋紡株式会社 核酸増幅の非特異反応を抑制する新規の方法
CN113981045B (zh) * 2021-11-23 2025-09-16 广州达安基因股份有限公司 制备恒温扩增混合酶系的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0614780A (ja) * 1992-06-30 1994-01-25 Takara Shuzo Co Ltd α型ＤＮＡポリメラーゼ遺伝子のクローニング方法
EP0810288A2 (fr) * 1996-05-03 1997-12-03 Guo Fan Hong Nouvelle ADN Polymérase avec une activité exonucléase de lecture de correction 3'-5'
US5874282A (en) * 1994-04-01 1999-02-23 Gen-Probe Incorporated Purified DNA polymerase from Bacillus stearothermophilus ATTC 12980

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5432065A (en) * 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0614780A (ja) * 1992-06-30 1994-01-25 Takara Shuzo Co Ltd α型ＤＮＡポリメラーゼ遺伝子のクローニング方法
US5874282A (en) * 1994-04-01 1999-02-23 Gen-Probe Incorporated Purified DNA polymerase from Bacillus stearothermophilus ATTC 12980
EP0810288A2 (fr) * 1996-05-03 1997-12-03 Guo Fan Hong Nouvelle ADN Polymérase avec une activité exonucléase de lecture de correction 3'-5'

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALIOTTA J. M. ET AL: "Thermostable Bst DNA polymerase I lacks a 3'--<5' proofreading exonuclease activity", GENET. ANAL., vol. 12, no. 5-6, 1996, pages 185 - 195, XP002943739 *
DATABASE GENBANK "Bacillus stearothermophilus DNA polymerase I (POLG1) gene complete cds", XP002981638, Database accession no. BSU93028 *
See also references of EP1436391A4 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023601B2 (en) 2002-09-12 2015-05-05 Gen9, Inc. Microarray synthesis and assembly of gene-length polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides

Also Published As

Publication number Publication date
CA2449560A1 (fr) 2002-12-19
EP1436391A4 (fr) 2005-02-16
RU2004100107A (ru) 2005-06-10
EP1436391A2 (fr) 2004-07-14
WO2002101004A2 (fr) 2002-12-19
JP2005514003A (ja) 2005-05-19

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