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WO2002100801A3 - Method for reversible immobilisation of biological macromolecules and device therefor - Google Patents

Method for reversible immobilisation of biological macromolecules and device therefor Download PDF

Info

Publication number
WO2002100801A3
WO2002100801A3 PCT/FR2002/002011 FR0202011W WO02100801A3 WO 2002100801 A3 WO2002100801 A3 WO 2002100801A3 FR 0202011 W FR0202011 W FR 0202011W WO 02100801 A3 WO02100801 A3 WO 02100801A3
Authority
WO
WIPO (PCT)
Prior art keywords
cyclodextrin
device therefor
biological macromolecules
reversible
macromolecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2002/002011
Other languages
French (fr)
Other versions
WO2002100801A2 (en
Inventor
Bernard Sebille
Marie-Claude Millot
Christelle David
Estelle Renard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to AU2002317245A priority Critical patent/AU2002317245A1/en
Publication of WO2002100801A2 publication Critical patent/WO2002100801A2/en
Anticipated expiration legal-status Critical
Publication of WO2002100801A3 publication Critical patent/WO2002100801A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/552—Glass or silica

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)

Abstract

The invention concerns a method for immobilising various molecules. The immobilisation method is reversible and essentially comprises steps which consist in: fixing a cyclodextrin or a cyclodextrin polymer on a substrate; producing a covalent bond between a hydrophilic polymer having hydrophobic groups and the biological molecule or macromolecule to be immobilised; contacting the biological molecule or macromolecule thus modified with the substrate bearing the cyclodextrin or the cyclodextrin polymer. The invention is particularly applicable to analytical techniques requiring regeneration of sensors.
PCT/FR2002/002011 2001-06-12 2002-06-12 Method for reversible immobilisation of biological macromolecules and device therefor Ceased WO2002100801A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002317245A AU2002317245A1 (en) 2001-06-12 2002-06-12 Method for reversible immobilisation of biological macromolecules and device therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0107700A FR2825700B1 (en) 2001-06-12 2001-06-12 REVERSIBLE IMMOBILIZATION METHOD FOR BIOLOGICAL MACROMOLECULES AND DEVICE FOR IMPLEMENTING SAME
FR01/07700 2001-06-12

Publications (2)

Publication Number Publication Date
WO2002100801A2 WO2002100801A2 (en) 2002-12-19
WO2002100801A3 true WO2002100801A3 (en) 2004-05-06

Family

ID=8864240

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/002011 Ceased WO2002100801A2 (en) 2001-06-12 2002-06-12 Method for reversible immobilisation of biological macromolecules and device therefor

Country Status (3)

Country Link
AU (1) AU2002317245A1 (en)
FR (1) FR2825700B1 (en)
WO (1) WO2002100801A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2970263B1 (en) * 2011-01-06 2013-02-08 Commissariat Energie Atomique BIOSENSOR FOR DETECTING THE PRESENCE OF PROTEASES AND POSSIBLY THE QUANTIFICATION OF THE ENZYMATIC ACTIVITY THEREOF

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4539399A (en) * 1984-07-27 1985-09-03 Advanced Separation Technologies Inc. Bonded phase material for chromatographic separations
US4781858A (en) * 1986-07-22 1988-11-01 Director General Of Agency Of Industrial Science And Technology Cyclodextrin-silica composite and a method for the preparation thereof
EP0445604A2 (en) * 1990-03-06 1991-09-11 MERCK PATENT GmbH Separation materials for chromatography
WO1992021769A1 (en) * 1991-05-30 1992-12-10 Abbott Laboratories Reagents containing a nonspecific binding blocker in ion-capture binding assays

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4539399A (en) * 1984-07-27 1985-09-03 Advanced Separation Technologies Inc. Bonded phase material for chromatographic separations
US4781858A (en) * 1986-07-22 1988-11-01 Director General Of Agency Of Industrial Science And Technology Cyclodextrin-silica composite and a method for the preparation thereof
EP0445604A2 (en) * 1990-03-06 1991-09-11 MERCK PATENT GmbH Separation materials for chromatography
WO1992021769A1 (en) * 1991-05-30 1992-12-10 Abbott Laboratories Reagents containing a nonspecific binding blocker in ion-capture binding assays

Also Published As

Publication number Publication date
AU2002317245A1 (en) 2002-12-23
WO2002100801A2 (en) 2002-12-19
FR2825700A1 (en) 2002-12-13
FR2825700B1 (en) 2003-09-05

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