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WO2002038734A3 - Procedes de determination des effets biologiques de composes sur l'expression genetique - Google Patents

Procedes de determination des effets biologiques de composes sur l'expression genetique Download PDF

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Publication number
WO2002038734A3
WO2002038734A3 PCT/US2001/046927 US0146927W WO0238734A3 WO 2002038734 A3 WO2002038734 A3 WO 2002038734A3 US 0146927 W US0146927 W US 0146927W WO 0238734 A3 WO0238734 A3 WO 0238734A3
Authority
WO
WIPO (PCT)
Prior art keywords
compound
methods
complexes
cells
nuclear extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/046927
Other languages
English (en)
Other versions
WO2002038734A2 (fr
Inventor
Christopher C Adams
Paul Labhart
Mary E Harper
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cistem Molecular Corp
Original Assignee
Cistem Molecular Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cistem Molecular Corp filed Critical Cistem Molecular Corp
Priority to CA002431047A priority Critical patent/CA2431047A1/fr
Priority to US10/250,802 priority patent/US20040048262A1/en
Priority to EP01992034A priority patent/EP1349961A4/fr
Priority to AU2002232510A priority patent/AU2002232510A1/en
Publication of WO2002038734A2 publication Critical patent/WO2002038734A2/fr
Publication of WO2002038734A3 publication Critical patent/WO2002038734A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4703—Regulators; Modulating activity

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne des procédés de détermination d'un ou plusieurs effets biologiques d'un composé sur l'expression génétique. Ces procédés consistent à obtenir un extrait nucléaire de cellules exposé à un composé, puis à combiner cet extrait avec un acide nucléique, ou une librairie d'acides nucléiques, contenant un ou plusieurs éléments régulateurs dans des conditions permettant la formation de complexes cis/trans entre les éléments régulateurs et les composants (par ex., les protéines de liaison d'ADN) de l'extrait nucléaire. Les complexes ainsi formés sont comparés aux complexes formés au moyen des extraits nucléaires obtenus de la même manière, mais dont les cellules n'ont pas été traitées, et au composé. Les différences entre les complexes formés après exposition des cellules au composé peuvent ensuite être évaluées. Les procédés de l'invention sont, de préférence, exécutés dans une configuration à débit élevé, et sont utiles, par exemple, afin d'évaluer l'efficacité, la toxicité, et le mécanisme d'action d'un composé. Par ailleurs, cette invention sera utile dans la mise au point de nouveaux médicaments, et dans la meilleure compréhension et amélioration de composés déjà utilisés ou en cours de mise au point.
PCT/US2001/046927 2000-11-13 2001-11-09 Procedes de determination des effets biologiques de composes sur l'expression genetique Ceased WO2002038734A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002431047A CA2431047A1 (fr) 2000-11-13 2001-11-09 Procedes de determination des effets biologiques de composes sur l'expression genetique
US10/250,802 US20040048262A1 (en) 2001-11-09 2001-11-09 Methods for determining the bilogical effects of compounds on gene expression
EP01992034A EP1349961A4 (fr) 2000-11-13 2001-11-09 Procedes de determination des effets biologiques de composes sur l'expression genetique
AU2002232510A AU2002232510A1 (en) 2000-11-13 2001-11-09 Methods for determining the biological effects of compounds on gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24833900P 2000-11-13 2000-11-13
US60/248,339 2000-11-13

Publications (2)

Publication Number Publication Date
WO2002038734A2 WO2002038734A2 (fr) 2002-05-16
WO2002038734A3 true WO2002038734A3 (fr) 2002-07-11

Family

ID=22938682

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046927 Ceased WO2002038734A2 (fr) 2000-11-13 2001-11-09 Procedes de determination des effets biologiques de composes sur l'expression genetique

Country Status (4)

Country Link
EP (1) EP1349961A4 (fr)
AU (1) AU2002232510A1 (fr)
CA (1) CA2431047A1 (fr)
WO (1) WO2002038734A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11493519B2 (en) 2019-02-08 2022-11-08 Dewpoint Therapeutics, Inc. Methods of characterizing condensate-associated characteristics of compounds and uses thereof
US12422427B2 (en) 2018-10-15 2025-09-23 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Compounds for treatment of diseases and methods of screening therefor

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040058356A1 (en) * 2001-03-01 2004-03-25 Warren Mary E. Methods for global profiling gene regulatory element activity
EP1402065B1 (fr) * 2001-06-08 2015-01-28 Affymetrix, Inc. Methode de detection d'interactions entre une proteine et un facteur de transcription
US7981842B2 (en) 2001-06-08 2011-07-19 Panomics, Inc. Method for detecting transcription factor-protein interactions
TW202003051A (zh) * 2018-03-23 2020-01-16 美商白頭生物醫學研究所 藉由調節凝聚物來調節基因轉錄之方法及分析
CN108548780A (zh) * 2018-03-30 2018-09-18 中国人民解放军第二军医大学 转录因子芯片试剂盒及高通量筛选靶基因转录因子的方法
CA3140651A1 (fr) * 2019-05-15 2020-11-19 Whitehead Institute For Biomedical Research Procedes de caracterisation et methodes d'utilisation d'interactions agent-condensat
EP4031876A1 (fr) 2019-09-18 2022-07-27 Dewpoint Therapeutics, Inc. Procédés de criblage de spécificité associée à un condensat et leurs utilisations
CN116801872A (zh) * 2021-02-10 2023-09-22 上海奕拓医药科技有限责任公司 调节雄激素受体凝聚物的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986055A (en) * 1997-11-13 1999-11-16 Curagen Corporation CDK2 interactions

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4312399A1 (de) * 1993-04-16 1994-10-20 Boehringer Mannheim Gmbh Verfahren zur Bestimmung der Bindung eines Transkriptionsfaktors an eine Nukleinsäure
US5821053A (en) * 1995-02-10 1998-10-13 Center For Blood Research, Inc. LIL-Stat DNA binding sites and methods for identifying inhibitory binding agents
US6066452A (en) * 1997-08-06 2000-05-23 Yale University Multiplex selection technique for identifying protein-binding sites and DNA-binding proteins
US6548021B1 (en) * 1997-10-10 2003-04-15 President And Fellows Of Harvard College Surface-bound, double-stranded DNA protein arrays
US6100035A (en) * 1998-07-14 2000-08-08 Cistem Molecular Corporation Method of identifying cis acting nucleic acid elements
EP1136567A1 (fr) * 2000-03-24 2001-09-26 Advanced Array Technologies S.A. Procédé et trousse pour l'identification, la détection et/ou la quantification des facteurs de transcription

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986055A (en) * 1997-11-13 1999-11-16 Curagen Corporation CDK2 interactions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOSS, V. ET AL.: "Nerve growth factor, but not epidermal growth factor, increases Fra-2 expression and alters Fra-2/JunD binding to AP-1 and CREB binding elements in phechromocytoma (PC12) cells", JOURNAL OF NEUROSCIENCE, vol. 21, 1 January 2001 (2001-01-01), pages 18 - 26, XP002909908 *
LEANNA, A. ET AL.: "The reverse two-hybrid system: a genetic scheme for selection against specific protein/protein interactions", NUCLEIC ACIDS RESEARCH, vol. 24, no. 17, 1996, pages 3341 - 3347, XP002909909 *
See also references of EP1349961A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12422427B2 (en) 2018-10-15 2025-09-23 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Compounds for treatment of diseases and methods of screening therefor
US11493519B2 (en) 2019-02-08 2022-11-08 Dewpoint Therapeutics, Inc. Methods of characterizing condensate-associated characteristics of compounds and uses thereof

Also Published As

Publication number Publication date
WO2002038734A2 (fr) 2002-05-16
EP1349961A2 (fr) 2003-10-08
AU2002232510A1 (en) 2002-05-21
CA2431047A1 (fr) 2002-05-16
EP1349961A4 (fr) 2005-01-26

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