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WO2002024872A8 - Manipulation de spermatozoides - Google Patents

Manipulation de spermatozoides

Info

Publication number
WO2002024872A8
WO2002024872A8 PCT/GB2001/004184 GB0104184W WO0224872A8 WO 2002024872 A8 WO2002024872 A8 WO 2002024872A8 GB 0104184 W GB0104184 W GB 0104184W WO 0224872 A8 WO0224872 A8 WO 0224872A8
Authority
WO
WIPO (PCT)
Prior art keywords
sperm
extracts
methods
nuclei
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/004184
Other languages
English (en)
Other versions
WO2002024872A3 (fr
WO2002024872A2 (fr
Inventor
Subhasis Banerjee
Geeta Nargand
Stuart Campbell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
St Georges Enterprises Ltd
Original Assignee
St Georges Enterprises Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Georges Enterprises Ltd filed Critical St Georges Enterprises Ltd
Priority to AU2001287897A priority Critical patent/AU2001287897A1/en
Publication of WO2002024872A2 publication Critical patent/WO2002024872A2/fr
Publication of WO2002024872A3 publication Critical patent/WO2002024872A3/fr
Anticipated expiration legal-status Critical
Publication of WO2002024872A8 publication Critical patent/WO2002024872A8/fr
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de perméabilisation de sperme consistant à : (a) laver un échantillon contenant du sperme dans un tampon d'extraction nucléaire de sperme (SNEB) comprenant un ou plusieurs agent(s) de chélation métallique et quasiment dépourvu(s) de détergents non ioniques et de cations divalents; (b) mettre en contact le sperme avec un ou plusieurs agent(s) de perméabilisation; et (c) éventuellement séparer la queue du sperme de sa tête. L'invention concerne également un procédé de transformation du sperme dont le procédé consiste à laisser incuber un échantillon de sperme ou des noyaux de sperme dans un ou plusieurs extrait(s) dépourvu(s) de cellule. L'invention concerne enfin des extraits et des tampons à utiliser avec ces procédés, des procédés de fabrication desdits extraits et des utilisations de noyaux de sperme transformé.
PCT/GB2001/004184 2000-09-19 2001-09-19 Manipulation de spermatozoides Ceased WO2002024872A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001287897A AU2001287897A1 (en) 2000-09-19 2001-09-19 Manipulation of spermatozoa

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0022981.5 2000-09-19
GB0022981A GB0022981D0 (en) 2000-09-19 2000-09-19 Manipulation of spermatozoa

Publications (3)

Publication Number Publication Date
WO2002024872A2 WO2002024872A2 (fr) 2002-03-28
WO2002024872A3 WO2002024872A3 (fr) 2002-08-08
WO2002024872A8 true WO2002024872A8 (fr) 2004-04-15

Family

ID=9899743

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/004184 Ceased WO2002024872A2 (fr) 2000-09-19 2001-09-19 Manipulation de spermatozoides

Country Status (3)

Country Link
AU (1) AU2001287897A1 (fr)
GB (1) GB0022981D0 (fr)
WO (1) WO2002024872A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7253334B2 (en) 2000-12-22 2007-08-07 Aurox, Llc Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7491534B2 (en) 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
US7125976B2 (en) 2003-07-01 2006-10-24 Trustees Of Columbia University In The City Of New York Method of screening for agents inhibiting chloride intracellular channels

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480772A (en) * 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
GB9402586D0 (en) * 1994-02-10 1994-04-06 Hulten Maj Methods for chromosome condensation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7253334B2 (en) 2000-12-22 2007-08-07 Aurox, Llc Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells

Also Published As

Publication number Publication date
WO2002024872A3 (fr) 2002-08-08
GB0022981D0 (en) 2000-11-01
AU2001287897A1 (en) 2002-04-02
WO2002024872A2 (fr) 2002-03-28

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