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WO2002014851A3 - Electrophoresis apparatus and method - Google Patents

Electrophoresis apparatus and method Download PDF

Info

Publication number
WO2002014851A3
WO2002014851A3 PCT/US2001/024271 US0124271W WO0214851A3 WO 2002014851 A3 WO2002014851 A3 WO 2002014851A3 US 0124271 W US0124271 W US 0124271W WO 0214851 A3 WO0214851 A3 WO 0214851A3
Authority
WO
WIPO (PCT)
Prior art keywords
dimension
electrophoresis
region
substantially perpendicular
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/024271
Other languages
French (fr)
Other versions
WO2002014851A2 (en
Inventor
John E Wiktorowicz
Yefim M Raysberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lynx Therapeutics Inc
Original Assignee
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Priority to AU2001277247A priority Critical patent/AU2001277247A1/en
Publication of WO2002014851A2 publication Critical patent/WO2002014851A2/en
Publication of WO2002014851A3 publication Critical patent/WO2002014851A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed are an apparatus and method for two-dimensional electrophoretic separation and subsequent isolation of analytes of interest, particularly polypeptides. The apparatus includes a sample separation cavity comprising a first electrophoresis region for performing charge and/or sized-based electrophoresis in a first dimension, and a second electrophoresis region for performing electrophoresis in a second dimension, in a direction substantially perpendicular to the first dimension. The second region also contains a plurality of elongate separation channels substantially perpendicular to the first dimension, and these channels contain a removable solid phase effective to immobilize separated components upon activation. Non-immobilized components can then be removed, and immobilized components recovered, without loss of resolution.
PCT/US2001/024271 2000-08-10 2001-08-03 Electrophoresis apparatus and method Ceased WO2002014851A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001277247A AU2001277247A1 (en) 2000-08-10 2001-08-03 Electrophoresis apparatus and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63621200A 2000-08-10 2000-08-10
US09/636,212 2000-08-10

Publications (2)

Publication Number Publication Date
WO2002014851A2 WO2002014851A2 (en) 2002-02-21
WO2002014851A3 true WO2002014851A3 (en) 2003-01-23

Family

ID=24550932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/024271 Ceased WO2002014851A2 (en) 2000-08-10 2001-08-03 Electrophoresis apparatus and method

Country Status (2)

Country Link
AU (1) AU2001277247A1 (en)
WO (1) WO2002014851A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9304133B2 (en) 2004-07-19 2016-04-05 ProteinSimple Methods and devices for analyte detection
US9400277B2 (en) 2004-07-19 2016-07-26 ProteinSimple Methods and devices for analyte detection

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
WO2007035864A2 (en) * 2005-09-20 2007-03-29 Cell Biosciences, Inc. Electrophoresis standards, methods and kits
US11237131B2 (en) * 2015-05-20 2022-02-01 ProteinSimple Systems and methods for electrophoretic separation and analysis of analytes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033412A1 (en) * 1995-04-20 1996-10-24 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
WO1999018438A1 (en) * 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
WO1999061901A1 (en) * 1998-05-22 1999-12-02 Lynx Therapeutics, Inc. Electrophoresis apparatus and method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033412A1 (en) * 1995-04-20 1996-10-24 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
WO1999018438A1 (en) * 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
WO1999061901A1 (en) * 1998-05-22 1999-12-02 Lynx Therapeutics, Inc. Electrophoresis apparatus and method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9304133B2 (en) 2004-07-19 2016-04-05 ProteinSimple Methods and devices for analyte detection
US9400277B2 (en) 2004-07-19 2016-07-26 ProteinSimple Methods and devices for analyte detection

Also Published As

Publication number Publication date
WO2002014851A2 (en) 2002-02-21
AU2001277247A1 (en) 2002-02-25

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