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WO2002004663A3 - A nucleic acid-based method for tree phenotype prediction - Google Patents

A nucleic acid-based method for tree phenotype prediction Download PDF

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Publication number
WO2002004663A3
WO2002004663A3 PCT/CA2001/000927 CA0100927W WO0204663A3 WO 2002004663 A3 WO2002004663 A3 WO 2002004663A3 CA 0100927 W CA0100927 W CA 0100927W WO 0204663 A3 WO0204663 A3 WO 0204663A3
Authority
WO
WIPO (PCT)
Prior art keywords
spruce
hybrid
population
fibre length
dna probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2001/000927
Other languages
French (fr)
Other versions
WO2002004663A2 (en
Inventor
Simon Potter
Paul A Watson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pulp and Paper Research Institute of Canada
Original Assignee
Pulp and Paper Research Institute of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pulp and Paper Research Institute of Canada filed Critical Pulp and Paper Research Institute of Canada
Priority to EP01949143A priority Critical patent/EP1326999A2/en
Priority to AU2001270387A priority patent/AU2001270387A1/en
Priority to CA002412852A priority patent/CA2412852A1/en
Publication of WO2002004663A2 publication Critical patent/WO2002004663A2/en
Anticipated expiration legal-status Critical
Publication of WO2002004663A3 publication Critical patent/WO2002004663A3/en
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H7/00—Gymnosperms, e.g. conifers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a novel method for the prediction of fibre length and the rapid selection of superior trees for given pulp and paper product lines using a DNA probe. The method comprises the isolation of tree genomic DNA from a hybrid spruce live tissue source, hybridization of the spruce DNA probe to that genomic DNA and the densitometric assessment of the intensity of the hybridization pattern obtained. This determines the precise degree of genetic admixing (or introgression) of the two parent species within the hybrid population. Due to the linear relationship - in the hybrid spruce population examined - between degree of genetic introgression and fibre length (discovered in this method), the intensity of the DNA probe hybridization pattern can be used to directly, accurately and reproducibly predict the fibre length found (for a given tree age) within an individual hybrid spruce within the population.
PCT/CA2001/000927 2000-06-23 2001-06-21 A nucleic acid-based method for tree phenotype prediction Ceased WO2002004663A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01949143A EP1326999A2 (en) 2000-06-23 2001-06-21 A nucleic acid-based method for tree phenotype prediction
AU2001270387A AU2001270387A1 (en) 2000-06-23 2001-06-21 A nucleic acid-based method for tree phenotype prediction
CA002412852A CA2412852A1 (en) 2000-06-23 2001-06-21 A nucleic acid-based method for tree phenotype prediction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21358500P 2000-06-23 2000-06-23
US60/213,585 2000-06-23

Publications (2)

Publication Number Publication Date
WO2002004663A2 WO2002004663A2 (en) 2002-01-17
WO2002004663A3 true WO2002004663A3 (en) 2003-05-01

Family

ID=22795673

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000927 Ceased WO2002004663A2 (en) 2000-06-23 2001-06-21 A nucleic acid-based method for tree phenotype prediction

Country Status (5)

Country Link
EP (1) EP1326999A2 (en)
CN (1) CN1451049A (en)
AU (1) AU2001270387A1 (en)
CA (1) CA2412852A1 (en)
WO (1) WO2002004663A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106811513B (en) * 2015-12-01 2020-12-01 中华人民共和国上海出入境检验检疫局 Real-time fluorescent PCR detection method and kit for eucalyptus components

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110296753A1 (en) * 2010-06-03 2011-12-08 Syngenta Participations Ag Methods and compositions for predicting unobserved phenotypes (pup)
CN102676649B (en) * 2012-02-29 2014-06-04 西北大学 Pinus bungeana paternal line identification method
CN103279636B (en) * 2013-04-10 2016-03-02 北京林业大学 A kind of method determining tree age
CN105274103B (en) * 2015-11-22 2018-05-25 保琦蓓 A kind of discriminating Chinese fiber crops, ramie, the PCR primer group of flax raw ramie kinds of fibers and PCR discrimination methods
CN108467900B (en) * 2017-02-23 2020-03-10 北京林业大学 Method and kit for screening poplar growth traits by jointly using lncRNA and target gene thereof and application
CN109727640B (en) * 2019-01-22 2021-03-02 隆平农业发展股份有限公司 Whole genome prediction method and device based on automatic machine learning technology
CN114486416A (en) * 2021-12-24 2022-05-13 华东师范大学 A kind of detection method of rot wood decomposition rate based on plant economic type spectrum

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995019697A1 (en) * 1994-01-21 1995-07-27 North Carolina State University Methods for within family selection in woody perennials using genetic markers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995019697A1 (en) * 1994-01-21 1995-07-27 North Carolina State University Methods for within family selection in woody perennials using genetic markers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRADSHAW H D ET AL: "MOLECULAR GENETICS OF GROWTH AND DEVELOPMENT IN POPULUS. IV. MAPPING QTLS WITH LARGE EFFECTS OF GROWTH, FORM, AND PHENOLOGY TRAITS IN A FOREST TREE", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 199, 1995, pages 963 - 973, XP000909821, ISSN: 0016-6731 *
TANKSLEY S D: "RFLP MAPPING IN PLANT BREEDING: NEW TOOLS FOR AN OLD SCIENCE", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 7, no. 3, 1 March 1989 (1989-03-01), pages 257 - 264, XP000368711, ISSN: 0733-222X *
TRUEMAN L J ET AL: "Molecular cloning of higher plant homologues of the high-affinity nitrate transporters of Chlamydomonas reinhardtii and Aspergillus nidulans", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 175, no. 1, 10 October 1996 (1996-10-10), pages 223 - 231, XP004043319, ISSN: 0378-1119 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106811513B (en) * 2015-12-01 2020-12-01 中华人民共和国上海出入境检验检疫局 Real-time fluorescent PCR detection method and kit for eucalyptus components

Also Published As

Publication number Publication date
AU2001270387A1 (en) 2002-01-21
CA2412852A1 (en) 2002-01-17
CN1451049A (en) 2003-10-22
EP1326999A2 (en) 2003-07-16
WO2002004663A2 (en) 2002-01-17

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