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WO2002000924A3 - Viral phospholipase a2 enzymes, anti-viral agents and methods of use - Google Patents

Viral phospholipase a2 enzymes, anti-viral agents and methods of use Download PDF

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Publication number
WO2002000924A3
WO2002000924A3 PCT/CA2001/000932 CA0100932W WO0200924A3 WO 2002000924 A3 WO2002000924 A3 WO 2002000924A3 CA 0100932 W CA0100932 W CA 0100932W WO 0200924 A3 WO0200924 A3 WO 0200924A3
Authority
WO
WIPO (PCT)
Prior art keywords
viral
pla2
present
phospholipase
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2001/000932
Other languages
French (fr)
Other versions
WO2002000924A2 (en
Inventor
Peter Tijssen
Zoltan Zadori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de La Recherche Scientifique INRS
Original Assignee
Institut National de La Recherche Scientifique INRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de La Recherche Scientifique INRS filed Critical Institut National de La Recherche Scientifique INRS
Priority to AU2001267239A priority Critical patent/AU2001267239A1/en
Publication of WO2002000924A2 publication Critical patent/WO2002000924A2/en
Publication of WO2002000924A3 publication Critical patent/WO2002000924A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a novel class of phospholipase A2 enzyme (PLA2) from viruses and a method for identifying, isolating, purifying and characterizing enzymes of this class. The present invention includes viral PLA2 proteins, nucleic acids, and antisense oligonucleotides, and the use of these molecules in screening methods for anti-viral agents, in decreasing the infectivity and/or replication of viruses and as research tools. The present invention further includes treatment or prevention of virus-associated diseases using viral PLA2 inhibitors and the use of the viral PLA2-encoding region to improve virus-based gene therapy vectors.
PCT/CA2001/000932 2000-06-28 2001-06-28 Viral phospholipase a2 enzymes, anti-viral agents and methods of use Ceased WO2002000924A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001267239A AU2001267239A1 (en) 2000-06-28 2001-06-28 Viral phospholipase a2 enzymes, anti-viral agents and methods of use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA2310263 2000-06-28
CA2,310,263 2000-06-28

Publications (2)

Publication Number Publication Date
WO2002000924A2 WO2002000924A2 (en) 2002-01-03
WO2002000924A3 true WO2002000924A3 (en) 2002-04-11

Family

ID=4166336

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000932 Ceased WO2002000924A2 (en) 2000-06-28 2001-06-28 Viral phospholipase a2 enzymes, anti-viral agents and methods of use

Country Status (2)

Country Link
AU (1) AU2001267239A1 (en)
WO (1) WO2002000924A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020742A1 (en) 2001-08-31 2003-03-13 Gen-Probe Incorporated Assay for detection of human parvovirus b19 nucleic acid
CN101522894B (en) * 2006-09-29 2012-07-04 绿色生命实验室有限公司 Recombinant Periplaneta melanogaster densovirus and its application
TW202045529A (en) 2019-02-15 2020-12-16 美商聖加莫治療股份有限公司 Compositions and methods for producing recombinant aav
CN110819737A (en) * 2019-09-02 2020-02-21 深圳芭卡生物科技有限公司 Primer and kit for detecting cat intestinal infection pathogens and application of primer and kit
CN112322791B (en) * 2020-11-27 2023-10-27 福建省农业科学院畜牧兽医研究所 A novel duck-dependent virus loop-mediated isothermal amplification detection primer set and kit
CN112852749A (en) * 2021-03-27 2021-05-28 哈尔滨元亨生物药业有限公司 Hybridoma cell strain C68 capable of efficiently secreting canine parvovirus monoclonal antibody and production method thereof by using bioreactor
CN114716567B (en) * 2022-03-01 2024-08-09 上海赛伦生物技术股份有限公司 Preparation method and application of chimeric antigen and preparation for resisting bungarose venom

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000028004A1 (en) * 1998-11-10 2000-05-18 The University Of North Carolina At Chapel Hill Virus vectors and methods of making and administering the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000028004A1 (en) * 1998-11-10 2000-05-18 The University Of North Carolina At Chapel Hill Virus vectors and methods of making and administering the same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BERGERON J ET AL: "Genome organization of the Kresse strain of porcine parvovirus: Identification of the allotropic determinant and comparison with those of NADL-2 and field isolates.", JOURNAL OF VIROLOGY, vol. 70, no. 4, 1996, pages 2508 - 2515, XP002184679, ISSN: 0022-538X *
DATABASE EMBL/GENBANK/DDBJ 23 May 2000 (2000-05-23), NONAKA, K. ET AL.: "Complete nucleotide sequence and genome organization of a newly isolated Bombyx densovirus, which is clearly different in ORF structure from BmDNV previously reported.", XP002184681 *
DATABASE EMBL/GENBANK/DDBJ 29 November 1994 (1994-11-29), TIJSSEN, P.: "Organization and expression of the ambisense genome of densonucleosis virus of Galleria mellonella (GmDNV)", XP002184680 *
ERDMAN DEAN D ET AL: "Genetic diversity of human parvovirus B19: Sequence analysis of the VP1/VP2 gene from multiple isolates.", JOURNAL OF GENERAL VIROLOGY, vol. 77, no. 11, 1996, pages 2767 - 2774, XP001037602, ISSN: 0022-1317 *

Also Published As

Publication number Publication date
WO2002000924A2 (en) 2002-01-03
AU2001267239A1 (en) 2002-01-08

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