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WO2002099031A1 - Dispositif et procede permettant de cultiver des micro-algues - Google Patents

Dispositif et procede permettant de cultiver des micro-algues Download PDF

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Publication number
WO2002099031A1
WO2002099031A1 PCT/JP2002/005263 JP0205263W WO02099031A1 WO 2002099031 A1 WO2002099031 A1 WO 2002099031A1 JP 0205263 W JP0205263 W JP 0205263W WO 02099031 A1 WO02099031 A1 WO 02099031A1
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WO
WIPO (PCT)
Prior art keywords
culture
culture vessel
microalgae
gas
cylinder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2002/005263
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English (en)
Japanese (ja)
Inventor
Toru Sato
Yoshihiro Tsuchiya
Shinsuke Usui
Seishiro Hirabayashi
Yutaka Kondo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yamaha Motor Co Ltd
Original Assignee
Yamaha Motor Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yamaha Motor Co Ltd filed Critical Yamaha Motor Co Ltd
Priority to JP2003502141A priority Critical patent/JP4079877B2/ja
Priority to KR1020037015758A priority patent/KR100609736B1/ko
Publication of WO2002099031A1 publication Critical patent/WO2002099031A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers

Definitions

  • the present invention relates to a closed-type microalgae culturing apparatus for culturing microalgae, which is a photosynthetic organism, and a microalgae culturing method.
  • Microalgae which are photosynthetic organisms, are cultivated as feed for aquaculture because they absorb carbon dioxide and produce useful components such as vitamins, amino acids, pigments, proteins, polysaccharides, and fatty acids by photosynthesis. ing.
  • this kind of microalgae is also used as a means for treating carbon dioxide, which is one of the causes of global warming, and in recent years, a culturing apparatus for culturing the carbon dioxide in a large amount has been studied.
  • a culture device is for culturing microalgae in a culture solution, and the light necessary for photosynthesis mainly uses sunlight, and carbon dioxide is air or a mixed gas of carbon dioxide and air. Supply by blowing into.
  • the capacity per installation area of a closed type cultivation apparatus is smaller than that of an open type cultivation method, and high concentration cultivation is necessary to increase high productivity.
  • microalgae adhere to the inner wall of the culture vessel, or microalgae form a colony in the culture vessel and precipitate. There is a problem of doing. Furthermore, precipitation of microalgae in the culture vessel becomes a breeding ground for bacteria and may cause the culture solution to spoil.
  • the present invention has been made in view of the above problems, and its purpose is to achieve sufficient productivity by achieving sufficient agitation of the culture solution, and to provide microalgae on the culture vessel wall.
  • An object of the present invention is to provide a microalgae culturing apparatus and a microalgae culturing method that can maintain high cultivation efficiency over a long period of time by preventing adhesion and precipitation on the bottom of a culture vessel.
  • the invention according to claim 1 includes, while introducing a culture solution into a culture vessel having an opening at the top, blowing a gas containing carbon dioxide into the culture solution,
  • the culture vessel is formed into a double cylindrical shape composed of a horizontally placed inner cylinder and an outer cylinder, and at least the outer cylinder is formed.
  • the tube is made of a transparent material that transmits visible light, and a gas inlet for blowing a gas for forming a swirling flow of the culture solution in the culture container is opened at a lower portion in the culture container. I do.
  • the invention described in claim 2 is the invention according to claim 1, wherein the inner cylinder and the outer cylinder are formed of a cylinder, an elliptical cylinder, or a long cylinder, and the inner cylinder and the outer cylinder are Are arranged concentrically or eccentrically.
  • the invention according to claim 3 is characterized in that the culture solution is put into a culture vessel having an opening at the top, and the gas containing carbon dioxide is blown into the culture solution, and visible light is incident on the culture solution.
  • an inner cylinder and an outer cylinder that are concentrically arranged are formed into a double cylinder, and at least the outer cylinder is made of a transparent material that transmits visible light.
  • a gas inlet opening at the lower part of the configured culture vessel is arranged on one of the right and left sides of a vertical plane passing through the center axis of the inner cylinder and the outer cylinder, and the gas is injected from the gas inlet.
  • a swirling flow of the culture solution is formed in the culture vessel.
  • the invention according to claim 4 is characterized in that the culture solution is put into a culture vessel having an opening at the top, and a gas containing carbon dioxide is blown into the culture solution, and visible light is incident on the culture solution.
  • a gas containing carbon dioxide is blown into the culture solution, and visible light is incident on the culture solution.
  • an inner cylinder and an outer cylinder that are eccentrically placed sideways are formed into a double cylinder, and at least the outer cylinder is made of a transparent material that transmits visible light.
  • a gas inlet opening at the lower part of the configured culture vessel is arranged on one of the right and left sides of a vertical plane passing through the center axis of the inner cylinder, and the gas is blown from the gas inlet to thereby culture the culture vessel.
  • a swirling flow of the culture solution is formed therein.
  • the invention according to claim 5 is the invention according to claim 3 or 4, wherein the gas inlets are arranged on right and left sides of a vertical plane passing through a center axis of the inner cylinder of the culture vessel.
  • the rotating direction of the culture solution in the culture container is alternately switched.
  • the invention described in claim 6 is the invention described in claim 3 or 4.
  • a plurality of gas inlets are arranged in a longitudinal direction of the culture vessel, and a gas is sequentially blown from the gas inlet at one end of the culture vessel with a predetermined time difference, so that the culture solution is introduced into the culture vessel.
  • the invention according to claim 7 is the invention according to claim 3 or 4, wherein a plurality of gas inlets pass through a central axis of the inner cylinder along a longitudinal direction of the culture vessel.
  • the method is characterized in that swirling flows of a culture solution having different directions in the longitudinal direction are alternately formed in the culture container by alternately arranging the gas on the left and right sides of a vertical plane and injecting gas from each gas inlet.
  • the invention described in claim 8 is the invention according to any one of claims 3 to 7, wherein water is supplied to the outer surface of the outer cylinder of the culture vessel with temperature-regulated water, The temperature of the culture solution is controlled by flowing temperature-regulated water into a water passage formed outside or flowing temperature-regulated water into the inner cylinder. Therefore, according to the first aspect of the present invention, a gas inlet for injecting a gas for forming a swirling flow of the culture solution into the culture container is opened at a lower portion in the culture container. To form a swirling flow of the culture solution in the culture vessel, and sufficiently agitate the culture solution so that all microalgae can be received fairly, thereby achieving high productivity. Can be.
  • the multi-phase turbulence during the passage of bubbles in the culture solution and the turbulent boundary layer on the wall surface and the Gertruder vortex caused by the flow of the culture solution along the curved wall of the double cylindrical culture vessel cause A vortex is generated from the curved wall to the curved wall of the inner cylinder and from the curved wall of the inner cylinder to the curved wall of the outer cylinder, and the vortex causes sufficient agitation without stagnation of the culture solution. Eliminates adhesion to wall surfaces or formation of colonies and sedimentation, and light transmission is not blocked by microalgae. Microalgae are efficiently cultured to efficiently and uniformly receive light. Thus, high culture efficiency can be maintained over a long period of time.
  • the culture vessel is composed of an inner cylinder and an outer cylinder with high pressure resistance, The thickness of the apparatus can be reduced, and the weight and cost of the apparatus can be reduced.
  • the culture vessel can be easily configured by arranging the inner cylinder and the outer cylinder, each of which is a cylinder, an elliptic cylinder, or a long cylinder, concentrically or eccentrically.
  • a culture vessel formed of a concentrically arranged inner cylinder and an outer cylinder in a double cylindrical shape, wherein at least the outer cylinder is made of a transparent material that transmits visible light.
  • a vortex is easily generated from the curved wall of the outer cylinder to the curved wall of the inner cylinder and from the curved wall of the inner cylinder to the curved wall of the outer cylinder, and the vortex sufficiently agitates the culture solution without stagnation.
  • the microalgae do not adhere to the wall surface of the culture vessel or form a colony and settle out, so that light transmission is not blocked by the microalgae, and the microalgae receive light efficiently and uniformly.
  • microalgae can be cultured efficiently, and high culture efficiency can be maintained for a long period of time.
  • a culture vessel formed of an eccentrically placed inner cylinder and an outer cylinder in a double cylindrical shape, wherein at least the outer cylinder is made of a transparent material that transmits visible light.
  • a gas inlet opening at the lower part is arranged on one of the right and left sides of a vertical plane passing through the center axis of the inner cylinder, and the gas is blown from the gas inlet to thereby inject the culture solution into the culture vessel. Since the swirling flow is formed, sufficient agitation of the culture solution is easily performed, so that all microalgae can be received fairly, thereby achieving high productivity.
  • the multi-phase turbulence when bubbles pass through the culture solution and the turbulent boundary layer on the wall ⁇ ⁇ By easily generating geller vortices due to the flow of the culture solution along the curved wall of the culture vessel having a double cylindrical shape, from the curved wall of the outer cylinder to the curved wall of the inner cylinder and from the curved wall of the inner cylinder A vortex is easily generated toward the curved wall of the outer cylinder, and the vortex allows the culture solution to be sufficiently agitated without stagnation, so that microalgae can adhere to the wall surface of the culture vessel or form a colony and precipitate. The microalgae are not obstructed, and the light transmission is not blocked by the microalgae.
  • the microalgae can be efficiently cultured to efficiently and uniformly receive the microalgae, and the high culture efficiency can be maintained for a long period of time. it can.
  • the gas inlets are arranged on the left and right sides of a vertical plane passing through the center axis of the inner cylinder of the culture vessel, and the two gas inlets are alternately cut at predetermined time intervals. By alternately switching the swirling direction of the culture solution in the culture container by switching, the culture solution can be stirred more efficiently.
  • a swirling flow of the culture solution in the culture vessel that changes along the longitudinal direction of the culture vessel sufficient agitation of the culture solution can be realized and high productivity can be obtained.
  • the temperature of the culture solution can be controlled by spraying or passing the temperature-regulated water to the culture vessel. Therefore, it is possible to keep the temperature at an appropriate level all year round, and it is possible to effectively eliminate the adverse effect on algal growth caused by excessive temperature rise of the culture solution particularly in summer.
  • FIG. 1 is a perspective view of a microalgae culturing apparatus according to the present invention.
  • FIG. 2 is a cutaway front view (a cutaway view in the direction of arrow A in FIG. 1) of the microalgae culture device according to the present invention.
  • FIG. 3 is a side sectional view of the microalgae culturing apparatus according to the present invention.
  • FIG. 4 is a sectional view taken along line BB of FIG.
  • FIG. 5 is a cross-sectional view showing another embodiment of the culture vessel of the microalgae culture apparatus according to the present invention.
  • FIG. 6 is a cross-sectional view showing another embodiment of the culture vessel of the microalgae culture apparatus according to the present invention.
  • FIG. 7 is a cross-sectional view showing another embodiment of the culture vessel of the microalgae culture apparatus according to the present invention.
  • FIG. 8 is a cross-sectional view showing another embodiment of the culture vessel of the microalgae culture apparatus according to the present invention.
  • FIG. 9 is a perspective view showing an example of actual production equipment using the microalgae culturing apparatus according to the present invention.
  • 1 is a microalgae cultivation apparatus
  • 4, 4', 4" is an inner cylinder
  • 4a, 4a ' is a gas inlet
  • 5b, 5b" is the gas inlet
  • 6 and 7 are the side walls
  • 11 is the culture solution
  • 14 is the gas introduction pipe
  • 17 is the gas discharge opening
  • 18 is the cap
  • Reference numeral 19 denotes a temperature control water introduction pipe.
  • FIG. 1 is a perspective view of the microalga culturing apparatus according to the present invention
  • FIG. FIG. 3 is a cross-sectional view of the microalgae culturing apparatus
  • FIG. 4 is a cross-sectional view taken along the line BB of FIG.
  • the microalga culturing apparatus 1 is configured by arranging a culture vessel 2 having a drum-like outer shape on a support base 3 horizontally.
  • the culture vessel 2 has a double cylindrical shape, and the left and right ends of an inner cylinder 4 and an outer cylinder 5 which are concentrically placed side by side by ring-shaped side walls 6 and 7. It is configured to be closed. That is, the side walls 6 and 7 are respectively incorporated into the left and right ends of the inner cylinder 4 and the outer cylinder 5 which are concentrically placed horizontally, and a plurality of holes (each in the illustrated example, 6) through a circular hole (not shown), pass a long port 8 horizontally (see Fig. 1), and tighten a nut 9 screwed to the end of each port 8 to form a drum-shaped culture vessel. 2 is assembled.
  • the long bonoleto 8 is arranged outside the outer cylinder 5, but may be arranged inside the inner cylinder 4.
  • a configuration may be adopted in which the side walls 6 and 7 are independently attached with short bolts and nuts screwed to the short bolts.
  • a spacer may be interposed between the inner cylinder 4 and the outer cylinder 5 to prevent bending. In this case, it is desirable to form a hole in the spacer.
  • an arc-shaped fixed bracket 10 (see FIGS. 1 and 2) along the outer shape of the side walls 6 and 7 is provided on the left and right upper portions of the frame-shaped support base 3.
  • the left and right side walls 6 and 7 are horizontally fixed and supported on the support base 3 by being fastened together with the fixing bracket 10 by the two ports 8 and the nuts 9 screwed to the two lower portions of the left and right side walls 6 and 7. ing.
  • a culture solution 11 is injected into a space surrounded by the inner cylinder 4 and the outer cylinder 5 and the side walls 6 and 7 formed in the culture vessel 2, and the liquid level is adjusted to the inner cylinder. It is kept higher than the top surface of 4.
  • the left and right ends of the inner cylinder 4 and the outer cylinder 5 are connected to both side walls 6 and 7 via seal members (not shown). Leakage to the outside is prevented.
  • the inner tube 4, the outer tube 5, and both side walls 6, 7 constituting the culture vessel 2 are made of a transparent material that transmits sunlight (visible light). And an acrylic resin is used.
  • the transparent material any material can be used as long as it has excellent light transmittance, high weather resistance and high UV resistance. Resin, glass, etc., such as recarbonate, polypropylene, polyethylene, and polyvinyl chloride can be selected.
  • the inner cylinder 4, the outer cylinder 5, and both side walls 6, 7 are formed of a transparent member.
  • at least the outer cylinder 5 is formed of a transparent member. It should be done.
  • a circular drain hole 5a (see FIG. 4) is formed at the lower portion in the center in the width direction on the side near one side wall 6 of the outer cylinder 5 of the culture vessel 2.
  • a drain pipe 12 is inserted into and connected to the drain hole 5a.
  • a drain valve 13 is provided in the middle of the drain pipe 12. By opening the drain valve 13, the culture solution 11 in the culture vessel 2 can be discharged to the outside.
  • a circular gas inlet 5b is drilled at three places in the longitudinal direction (either left or right of the surface Fv).
  • a gas introduction pipe 14 extends horizontally in the longitudinal direction below the culture vessel 2, and three branches branching from the gas introduction pipe 14 and extending toward the culture vessel 2.
  • the tubes 15 are respectively inserted into and bound to the respective gas blowing ports 5b formed in the lower part of the outer cylinder 5 of the culture vessel 2.
  • the gas introduction pipe 14 is connected to a gas supply source such as a compressor for supplying air or a mixed gas of carbon dioxide and air.
  • a cylindrical gas discharge tube 16 is attached to the top of the culture vessel 2 (outer cylinder), and an inside of the culture vessel 2 is formed with a gas discharge opening 17 that opens into the culture vessel 2.
  • An inverted dish-shaped cap 18 that opens downward is attached to the upper part of the gas discharge cylinder 16.
  • the gas discharge opening 17 is covered by a cap 18. It is possible to prevent contamination of the culture solution 11 in the culture vessel 2 with dust and dirt or floating microorganisms in the air. The same effect can be obtained by providing a filter in the gas discharge opening 17 instead of the cap 18.
  • a temperature-regulated water introduction pipe 19 is provided horizontally on the left and right sides of the gas discharge cylinder 16 at the upper part of the culture vessel 2 so as to be parallel to the length direction. Temperature control water The introduction pipe 19 is supported by a pair of left and right support brackets 20 attached to the upper portions of the left and right side walls 6 and 7. At the lower part of each temperature control water introduction pipe 19, a plurality of water spouts 19a are drilled as shown in Fig. 3, and the temperature control water introduction pipe 19 is not shown such as a cooling water pump. Connected to a temperature-regulated water supply. Next, the operation of the microalgae culturing apparatus 1 having the above configuration will be described.
  • the microalgae culturing apparatus 1 is installed outdoors, and the microalgae to be cultured and the culture solution 11 are put in the culture vessel 2, and a gas supply source (not shown) is driven to supply a gas containing carbon dioxide (air or carbon dioxide).
  • a gas supply source (not shown) is driven to supply a gas containing carbon dioxide (air or carbon dioxide).
  • gas flows through the gas introduction pipe 14, the gas is supplied from the three branch pipes 15 into the culture vessel 2.
  • the gas supplied into the culture vessel 2 forms bubbles as shown in Fig. 4 from the three bottoms of the culture vessel 2 and rises in the culture vessel 2, and in the process, the microalgae in the culture solution 11 are oxidized by the microalgae. Supply carbon. Due to the rise of the gas bubbles, a flow of the culture solution 11 swirling in the same direction (counterclockwise in FIG. 4) is formed in the culture vessel 2 as shown by an arrow in FIG. .
  • the microalgae in the culture vessel 2 undergo vitamins, amino acids, pigments, and proteins by photosynthesis. It manufactures useful components such as polysaccharides and fatty acids, and absorbs carbon dioxide, which contributes to global warming. Oxygen generated by the photosynthetic action is discharged to the atmosphere through a gas discharge opening 17 formed at the top of the culture vessel 2 and a gap between the gas discharge tube 16 and the cap 18.
  • an artificial light source can be installed in the center of the inner cylinder 4 of the culture vessel 2, and the microalgae can continuously perform photosynthesis throughout the day and night. Growth is promoted.
  • the temperature control water supply source is driven to flow the temperature control water (cooling water) to the temperature control water introduction pipe 19, and the temperature control water is drilled in the temperature control water introduction pipe 19 From the multiple sprinkler outlets 19a and flow along the outer surface of the outer cylinder 5 to cool the culture 11 in the culture vessel 2 and control the temperature of the culture 11 to control the temperature.
  • Temperature can be maintained year-round regardless of temperature, and especially in the summer, algae growth This can effectively eliminate the adverse effect on the vehicle.
  • the outer cylinder 5 The temperature of the culture solution 11 was controlled by spraying the temperature-adjusted water to the outer surface, but the temperature was adjusted to the water passage (not shown) formed outside the outer cylinder 11 Similarly, the same effect can be obtained by controlling the temperature of the culture solution 11 by passing the water or the temperature-regulated water into the inner cylinder 4.
  • the microalgae culturing apparatus 1 since the swirling flow of the culture solution 11 is formed in the culture vessel 2 by blowing gas, sufficient stirring of the culture solution 11 is performed. As a result, all microalgae can receive light fairly, and thereby high productivity can be achieved.
  • the multi-phase turbulence caused by the passage of bubbles in the culture solution 11 and the turbulent boundary layer on the wall surface and the Gellar vortex caused by the flow of the culture solution 11 along the curved wall of the double cylindrical culture vessel 2 As a result, a vortex is generated from the curved wall of the outer cylinder 5 to the curved wall of the inner cylinder 4 and from the curved wall of the inner cylinder 4 to the curved wall of the outer cylinder 5, and the vortex does not cause the culture solution 11 to stay. Since the algae are sufficiently stirred, the microalgae do not adhere to the wall of the culture vessel 2 or form a colony and settle, and light transmission is not blocked by the microalgae. Microalgae can be efficiently cultured to receive light uniformly, and high culture efficiency can be maintained over a long period of time.
  • microalgae adhere to the wall surface of the culture vessel 2 or form a colony and settle, it is not preferable because light reception of the microalgae is hindered, but according to the microalgae culture device 1, different types of mixed-phase turbulence and turbulence Since a flow boundary layer and a Gertler vortex (described in detail below) are generated, a vortex or turbulence is generated between the inner cylinder 4 and the outer cylinder 5, so that light transmission is not blocked by the microalgae.
  • Multiphase turbulence Turbulence caused by bubbles moving in the liquid phase
  • Turbulent boundary layer When a flow passes near a wall, the Reynolds number, a parameter representing the similar side of the flow, increases (the flow over the wall increases, or the distance that the flow contacts the wall increases).
  • the boundary layer which is a slow layer formed near the wall surface, becomes turbulent. This turbulent layer is called a turbulent boundary layer.
  • Görtler vortex When there is a flow parallel to a curved surface on a concave surface, a parameter expressing the similarity law of the flow When the Reynolds number, which is a meter, increases (the flow over the wall increases or the distance the flow contacts the wall increases), a rotating vortex perpendicular to the flow is generated. This rotating vortex is called Gel-Toller Evil. Further, since the culture vessel 2 is composed of the inner cylinder 4 and the outer cylinder 5 having high pressure resistance, the thickness of the culture vessel 1 can be suppressed to be small, and the weight and cost of the culture apparatus 1 can be reduced.
  • the culture vessel 2 can be easily configured by arranging the inner cylinder 4 and the outer cylinder 5 formed of cylinders concentrically.
  • the gas inlet 5b is located below the horizontal plane FH passing through the central axis of the inner cylinder 4 and the outer cylinder 5, and Since it is arranged on one of the left and right sides of the vertical plane Fv passing through the central axis, the flow of the culture solution 11 that rotates in one direction in the culture vessel 2 can be easily formed, and the multi-phase turbulent flow and turbulent boundary layer The generation of Gertler vortices is easy. If the gas inlet 5b is formed on the opposite side of the vertical plane as shown in FIG. 5, the flow of the culture solution 11 rotating in the opposite direction (the clockwise direction in FIG. 5) to the present embodiment is prevented. Can be formed.
  • gas inlets are formed on the left and right sides of a vertical plane passing through the center axis of the inner and outer cylinders, and the two inlets are alternately switched at predetermined time intervals.
  • the swirling direction can be switched alternately, and the culture solution can be stirred more efficiently.
  • the swirling direction of the culture solution 11 may be partially or transiently changed in the longitudinal direction of the culture vessel 2.
  • a plurality of gas inlets 5b are arranged in the longitudinal direction of the culture vessel 2, and gas is sequentially blown from the gas inlet 5b at one end of the culture vessel 2 with a predetermined time difference into the culture vessel 2.
  • a swirling flow that changes along the longitudinal direction of the culture vessel 2 of the culture solution 11 may be formed.
  • a plurality of gas inlets 5b are alternately arranged along the longitudinal direction of the culture vessel 2 on the left and right sides of a vertical plane passing through the center axis of the inner cylinder, and gas is blown from each gas inlet 5b.
  • a swirling flow of the culture solution 11 whose direction is alternately different in the longitudinal direction may be formed therein.
  • the culture vessel 2 is configured by concentrically arranging the inner cylinder 4 and the outer cylinder 5 formed of cylinders.
  • the culture vessel 2 may be configured by eccentrically disposing the As shown in the figure, if the air inlet 4a is arranged below the horizontal plane FH passing through the center axis of the inner cylinder 4 and at one of the right and left sides of the vertical plane Fv passing through the center axis as shown in the drawing, A flow of the culture solution 11 swirling in the same direction (counterclockwise in the illustrated example) can be easily formed, and a multiphase turbulent flow, a turbulent boundary layer, and a Gertler vortex can be easily generated.
  • a culture vessel 2 ' is constructed by concentrically arranging an inner cylinder 4' and an outer cylinder 5 'made of an elliptical cylinder as shown in FIG. 7, or an inner cylinder made of a long cylinder as shown in FIG. 4 "and the outer cylinder 5" may be arranged concentrically to form the culture vessel 2 ".
  • the gas inlets 4a 'and 5b" are connected to the inner cylinder 4' and 4 ".
  • a flow of the culture solution 11 that rotates in the counterclockwise direction can be formed.
  • the eccentric arrangement of the inner cylinder and the outer cylinder made of an elliptical cylinder or a long cylinder is not shown.
  • the culture vessel may be formed, and in these cases, the gas blowing port may be below the horizontal plane passing through the center axis of the cylinder and passing through the same center axis. It is possible to form a flow of the culture liquid to pivot in the same direction within the culture vessel by placing one left or right face.
  • FIG. 9 an example of an actual production facility using the microalgae culturing apparatus 1 according to the present embodiment is shown in FIG. 9, but in the actual production facility, a plurality of microalgae culturing apparatuses 1 are arranged in a row as shown in the figure. Are connected over several rows. In this case, in each row, one gas introduction pipe 14 and two temperature control water introduction pipes 19 are shared for each culture apparatus 1. Next, the results of a culture experiment performed using the microalgae culture apparatus according to the present invention will be described.
  • a culture solution is put into a culture vessel having an opening at the top, and visible light is emitted while blowing gas containing carbon dioxide into the culture solution.
  • the culture vessel is formed into a double cylindrical shape comprising a horizontally placed inner cylinder and an outer cylinder, and at least the outer cylinder is made of visible light.
  • a swirling flow of the culture solution is formed in the culture vessel by blowing the gas, thereby achieving sufficient agitation of the culture solution to obtain high productivity.
  • a culture solution is put into a culture container having an opening at a top portion, and while a gas containing carbon dioxide is blown into the culture solution, visible light is incident on the culture solution so that the inside of the culture container is formed.
  • a culture vessel formed of a concentrically arranged inner cylinder and an outer cylinder into a double cylinder and at least an outer cylinder made of a transparent material that transmits visible light
  • the gas inlet opening at the lower part of the cylinder is placed on one of the left and right sides of the vertical plane passing through the center axis of the inner cylinder and the outer cylinder, and the gas is blown from the gas inlet, or eccentrically placed horizontally

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Abstract

L'invention concerne un dispositif (1) et un procédé permettant de cultiver des micro-algues et d'obtenir une productivité élevée grâce à un mélange suffisant de la solution de culture et au maintien d'une efficacité élevée de la culture sur une longue période, en empêchant les micro-algues d'adhérer à la paroi d'un contenant de culture et de précipiter sur le fond de ce contenant de culture. Le dispositif de culture de micro-algues (1) permet la culture de micro-algues dans le contenant de culture (2), par remplissage de la solution de culture (11) dans le contenant de culture (2) qui possède une ouverture de décharge de gaz (17) au niveau d'une partie supérieure et par passage d'un faisceau lumineux visible dans la solution de culture (11) tout en insufflant un gaz contenant du dioxyde de carbone. Le contenant de culture (2) possède une structure tubulaire double, qui consiste en des tubes horizontaux intérieur et extérieur (4) et (5), au moins le tube extérieur (5) étant formé d'un matériau transparent permettant que le faisceau de lumière visible soit transmis à travers lui, et en un orifice d'entrée de gaz (5b) s'ouvrant sur la partie fond intérieure du contenant de culture (2). Le flux tourbillonnant de la solution de culture (11) peut être formé dans le contenant de culture (2) en insufflant le gaz à partir de l'orifice d'entrée de gaz (5b) dans le contenant de culture.
PCT/JP2002/005263 2001-06-01 2002-05-30 Dispositif et procede permettant de cultiver des micro-algues Ceased WO2002099031A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2003502141A JP4079877B2 (ja) 2001-06-01 2002-05-30 微細藻類培養装置、及び、微細藻類培養方法
KR1020037015758A KR100609736B1 (ko) 2001-06-01 2002-05-30 미세조류 배양장치 및 미세조류 배양방법

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JP2001-166956 2001-06-01
JP2001166956 2001-06-01

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WO2002099031A1 true WO2002099031A1 (fr) 2002-12-12

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KR (1) KR100609736B1 (fr)
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WO (1) WO2002099031A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009106218A (ja) * 2007-10-31 2009-05-21 Yukio Yoneda 光合成ユニット装置
JP2013521783A (ja) * 2010-03-12 2013-06-13 ソリックス バイオシステムズ インコーポレイテッド フレキシブルなフローティング式光バイオリアクターを配置するためのシステムおよび方法
WO2014202159A1 (fr) * 2013-06-20 2014-12-24 Biotubes Gmbh Canalisation destinée à être utilisée dans un photobioréacteur
JP2019129763A (ja) * 2018-01-31 2019-08-08 株式会社熊谷組 微細藻類培養装置

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100679989B1 (ko) * 2005-10-18 2007-02-08 한국생명공학연구원 접종물 배양조가 내부에 설치된 수로식 미세조류 옥외배양조
WO2011081234A1 (fr) * 2009-12-30 2011-07-07 강원대학교 산학협력단 Procédé de culture de micro-algues marines à l'aide des effluents provenant d'une centrale nucléaire
US8658421B2 (en) 2010-07-23 2014-02-25 Kairos Global Co., Ltd. Circulatory photobioreactor
EP2412794A1 (fr) 2010-07-30 2012-02-01 Kairos Global Co., Ltd. Procédé de culture circulatoire de micro-algues photosynthétiques
EP2412793A1 (fr) 2010-07-30 2012-02-01 Kairos Global Co., Ltd. Photobioréacteur circulatoire
CN102268362B (zh) * 2011-06-10 2013-07-24 薛命雄 螺旋藻培养管道式二氧化碳补碳装置及补碳方法
CN102660449A (zh) * 2012-04-27 2012-09-12 天津大学 套管式光生物反应器
CN102660448A (zh) * 2012-04-27 2012-09-12 天津大学 利用废气废热规模化培养微藻的套管式光生物反应系统
DE102012214493A1 (de) * 2012-08-14 2014-02-20 Air-Lng Gmbh Photobioreaktor zur Kultivierung von phototrophen Organismen
CN102911856B (zh) * 2012-10-29 2015-04-22 天津大学 一种适于微藻高效培养的相切套管内置曝气光生物反应器
KR101680110B1 (ko) 2015-02-06 2016-11-28 주식회사 클로랜드 공기 교환식 미세조류 고밀도 배양장치
KR101657489B1 (ko) 2015-02-13 2016-09-19 주식회사 클로랜드 공기를 이용한 순환형 미세조류 고밀도 배양장치
KR101657490B1 (ko) 2015-02-23 2016-09-30 주식회사 클로랜드 공기를 이용한 순환형 미세조류 고밀도 배양장치의 운용방법
KR102003634B1 (ko) 2018-02-01 2019-07-24 주식회사 레이바이오 광적외선을 이용한 미세조류 배양장치
CN114540162B (zh) * 2022-03-24 2024-07-26 衢州学院 封闭式微藻培养装置
KR102741954B1 (ko) * 2023-08-24 2024-12-16 주식회사 비루트랩 공기전달식 미세조류 광생물 반응기 및 이를 포함하는 배양시스템

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03112481A (ja) * 1989-09-26 1991-05-14 Hiroyuki Kikuchi 藻類の培養装置
WO1995019424A1 (fr) * 1994-01-12 1995-07-20 Yeda Research And Development Co. Ltd. Bioreacteur et systeme permettant une productivite amelioree d'algues photosynthetiques

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11509402A (ja) * 1995-02-02 1999-08-24 アスピタリア エッセ．エレ．エレ． 閉回路内で微小藻類を培養する方法と装置
ATE306545T1 (de) * 1998-03-31 2005-10-15 Bioreal Inc Kulturvorrichtung für feinalgen
CN2351950Y (zh) * 1998-09-01 1999-12-08 中国科学院海洋研究所 藻类生物反应器
CN1376777A (zh) * 2001-03-26 2002-10-30 中国科学院化工冶金研究所 微藻细胞溶剂化补碳与气浮法采收相耦合的培养方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03112481A (ja) * 1989-09-26 1991-05-14 Hiroyuki Kikuchi 藻類の培養装置
WO1995019424A1 (fr) * 1994-01-12 1995-07-20 Yeda Research And Development Co. Ltd. Bioreacteur et systeme permettant une productivite amelioree d'algues photosynthetiques

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009106218A (ja) * 2007-10-31 2009-05-21 Yukio Yoneda 光合成ユニット装置
JP2013521783A (ja) * 2010-03-12 2013-06-13 ソリックス バイオシステムズ インコーポレイテッド フレキシブルなフローティング式光バイオリアクターを配置するためのシステムおよび方法
US9145539B2 (en) 2010-03-12 2015-09-29 Solix Algredients, Inc. Systems and methods for positioning flexible floating photobioreactors
WO2014202159A1 (fr) * 2013-06-20 2014-12-24 Biotubes Gmbh Canalisation destinée à être utilisée dans un photobioréacteur
JP2019129763A (ja) * 2018-01-31 2019-08-08 株式会社熊谷組 微細藻類培養装置
JP7057148B2 (ja) 2018-01-31 2022-04-19 株式会社熊谷組 微細藻類培養装置

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KR100609736B1 (ko) 2006-08-08
CN1304555C (zh) 2007-03-14
KR20040019298A (ko) 2004-03-05
JPWO2002099031A1 (ja) 2004-09-16
CN1513055A (zh) 2004-07-14

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