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WO2002081656A3 - Potentiel diagnostique ameliore de cellules exprimant l'antigene specifique de la prostate - Google Patents

Potentiel diagnostique ameliore de cellules exprimant l'antigene specifique de la prostate Download PDF

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Publication number
WO2002081656A3
WO2002081656A3 PCT/US2002/009736 US0209736W WO02081656A3 WO 2002081656 A3 WO2002081656 A3 WO 2002081656A3 US 0209736 W US0209736 W US 0209736W WO 02081656 A3 WO02081656 A3 WO 02081656A3
Authority
WO
WIPO (PCT)
Prior art keywords
prostate
methods
epithelial cells
expression
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/009736
Other languages
English (en)
Other versions
WO2002081656A2 (fr
Inventor
Chun L Gao
Judd W Moul
Shiv Srivastava
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henry M Jackson Foundation for Advancedment of Military Medicine Inc
Original Assignee
Henry M Jackson Foundation for Advancedment of Military Medicine Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henry M Jackson Foundation for Advancedment of Military Medicine Inc filed Critical Henry M Jackson Foundation for Advancedment of Military Medicine Inc
Priority to AU2002245727A priority Critical patent/AU2002245727A1/en
Publication of WO2002081656A2 publication Critical patent/WO2002081656A2/fr
Publication of WO2002081656A3 publication Critical patent/WO2002081656A3/fr
Priority to US10/677,495 priority patent/US20040241707A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes et des kits sensibles et spécifiques utilisés dans le dépistage du cancer de la prostate chez un patient. L'invention utilise la technique de RT-PCR pour détecter l'expression ou le changement d'expression de l'antigène prostatique spécifique (PSA) dans les cellules épithéliales enrichies en sang entier. Ces méthodes et ces kits sont nettement améliorés par rapport aux méthodes antérieures de diagnostic du cancer de la prostate (CaP). Selon un premier mode de réalisation, les cellules épithéliales de la prostate sont isolées du sang et l'ARN soumis à la technique de RT-PCR. L'ADNc obtenu est soumis à l'amplification PCR via des amorces capables de distinguer entre la copie génomique du gène et la copie d'ADNc résultant de l'expression génique du CaP. L'invention concerne en outre un test plus sensible, plus spécifique et plus reproductible que les méthodes classiques. Les résultats de l'invention démontrent l'existence d'une corrélation entre la présence de cellules épithéliales de la prostate exprimant le PSA et la récurrence du cancer ; on obtient ainsi un outil diagnostique pour la détection précoce des maladies prostatiques.
PCT/US2002/009736 2001-04-05 2002-04-01 Potentiel diagnostique ameliore de cellules exprimant l'antigene specifique de la prostate Ceased WO2002081656A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002245727A AU2002245727A1 (en) 2001-04-05 2002-04-01 Enhanced diagnostic potential of prostate-specific antigen expressing cells
US10/677,495 US20040241707A1 (en) 2002-04-01 2003-10-03 Enhanced diagnostic potential of prostate-specific antigen expressing cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28137801P 2001-04-05 2001-04-05
US60/281,378 2001-04-05

Publications (2)

Publication Number Publication Date
WO2002081656A2 WO2002081656A2 (fr) 2002-10-17
WO2002081656A3 true WO2002081656A3 (fr) 2003-09-04

Family

ID=23077036

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/009736 Ceased WO2002081656A2 (fr) 2001-04-05 2002-04-01 Potentiel diagnostique ameliore de cellules exprimant l'antigene specifique de la prostate

Country Status (2)

Country Link
AU (1) AU2002245727A1 (fr)
WO (1) WO2002081656A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1540002A2 (fr) * 2002-09-12 2005-06-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Procede de detection de micro-metastases
JP2006072656A (ja) * 2004-09-01 2006-03-16 Hitachi Software Eng Co Ltd リアルタイムｐｃｒのプライマー設計方法
EP2407554A1 (fr) * 2010-07-14 2012-01-18 Fundacio Institut de Recerca de l'Hospital Universitari Vall d'Hebron Procédés et kits pour le diagnostic du cancer de la prostate
EP2407555A1 (fr) * 2010-07-14 2012-01-18 Fundació Institut de Recerca Hospital Universitari Vall d'Hebron, Fundació Privada Procédés et kits pour le diagnostic du cancer de la prostate

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021042A2 (fr) * 1995-01-04 1996-07-11 Trustees Of Boston University Amorces destinees a l'amplification pcr de sequences metastatiques
US5674682A (en) * 1992-10-29 1997-10-07 Thomas Jefferson University Nucleic acid primers for detecting micrometastasis of prostate cancer
US5858673A (en) * 1996-06-24 1999-01-12 Charlotte-Mecklenburg Hospital Authority Method for detecting prostate cells
US5976794A (en) * 1994-04-15 1999-11-02 The Trustees Of Columbia University In The City Of New York Method for molecular staging of prostate cancer
US6365362B1 (en) * 1998-02-12 2002-04-02 Immunivest Corporation Methods and reagents for the rapid and efficient isolation of circulating cancer cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674682A (en) * 1992-10-29 1997-10-07 Thomas Jefferson University Nucleic acid primers for detecting micrometastasis of prostate cancer
US5976794A (en) * 1994-04-15 1999-11-02 The Trustees Of Columbia University In The City Of New York Method for molecular staging of prostate cancer
WO1996021042A2 (fr) * 1995-01-04 1996-07-11 Trustees Of Boston University Amorces destinees a l'amplification pcr de sequences metastatiques
US5858673A (en) * 1996-06-24 1999-01-12 Charlotte-Mecklenburg Hospital Authority Method for detecting prostate cells
US6365362B1 (en) * 1998-02-12 2002-04-02 Immunivest Corporation Methods and reagents for the rapid and efficient isolation of circulating cancer cells

Also Published As

Publication number Publication date
WO2002081656A2 (fr) 2002-10-17
AU2002245727A1 (en) 2002-10-21

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