[go: up one dir, main page]

WO2002068684A3 - Méthode d'extension d'amorce spécifique d'allèle - Google Patents

Méthode d'extension d'amorce spécifique d'allèle Download PDF

Info

Publication number
WO2002068684A3
WO2002068684A3 PCT/GB2002/000794 GB0200794W WO02068684A3 WO 2002068684 A3 WO2002068684 A3 WO 2002068684A3 GB 0200794 W GB0200794 W GB 0200794W WO 02068684 A3 WO02068684 A3 WO 02068684A3
Authority
WO
WIPO (PCT)
Prior art keywords
primer extension
base
allele
primer
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2002/000794
Other languages
English (en)
Other versions
WO2002068684A2 (fr
WO2002068684A8 (fr
Inventor
Joakim Lundeberg
Afshin Ahmadian
Paal Nyren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotage AB
Original Assignee
Pyrosequencing AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/791,190 external-priority patent/US20030104372A1/en
Priority claimed from GB0104560A external-priority patent/GB0104560D0/en
Application filed by Pyrosequencing AB filed Critical Pyrosequencing AB
Priority to AU2002233540A priority Critical patent/AU2002233540A1/en
Publication of WO2002068684A2 publication Critical patent/WO2002068684A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002068684A8 publication Critical patent/WO2002068684A8/fr
Publication of WO2002068684A3 publication Critical patent/WO2002068684A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes d'extension d'amorce spécifique d'allèle utiles dans la détection de mutations et de variations génétiques. L'invention concerne, plus particulièrement, une méthode de détection d'une base au niveau d'une position prédéfinie dans une molécule d'acide nucléique. La méthode de l'invention consiste: à effectuer des réaction d'extension d'amorce au moyen d'amorces de détection à spécificité de base, chaque amorce étant spécifique pour une base particulière au niveau de la position prédéfinie, et à comparer ces extensions d'amorce afin de déterminer quelle base est présente au niveau de cette position, les réactions d'extension d'amorce étant effectuées au moyen de nucléotides marqués et une enzyme de dégradation de nucléotide étant présente durant la réaction d'extension d'amorce.
PCT/GB2002/000794 2001-02-23 2002-02-22 Méthode d'extension d'amorce spécifique d'allèle Ceased WO2002068684A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002233540A AU2002233540A1 (en) 2001-02-23 2002-02-22 Allele-specific primer extension assay

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0104560.8 2001-02-23
US09/791,190 US20030104372A1 (en) 1996-12-23 2001-02-23 Allele specific primer extension
GB0104560A GB0104560D0 (en) 2001-02-23 2001-02-23 Method
US09/791,190 2001-02-23
US7192602A 2002-02-07 2002-02-07
US10/071,926 2002-02-07

Publications (3)

Publication Number Publication Date
WO2002068684A2 WO2002068684A2 (fr) 2002-09-06
WO2002068684A8 WO2002068684A8 (fr) 2003-12-04
WO2002068684A3 true WO2002068684A3 (fr) 2007-12-27

Family

ID=27256081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000794 Ceased WO2002068684A2 (fr) 2001-02-23 2002-02-22 Méthode d'extension d'amorce spécifique d'allèle

Country Status (2)

Country Link
AU (1) AU2002233540A1 (fr)
WO (1) WO2002068684A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1620568T3 (da) 2003-04-24 2009-03-30 Afshin Ahmadian Analyse til allelspecifik mutationspåvisning
EP2298785B1 (fr) * 2004-02-06 2012-07-25 Canadian Blood Services Détermination simultanée de groupe sanguin et de génotypes d'antigène de plaquette
GB0600116D0 (en) * 2006-01-05 2006-02-15 Univ Cardiff Allele-specific sequencing
WO2011059285A2 (fr) * 2009-11-16 2011-05-19 (주)지노믹트리 Méthode de génotypage
US9803236B2 (en) 2010-08-06 2017-10-31 Tsinghua University Microarray-based assay integrated with particles for analyzing molecular interactions
US9310375B2 (en) 2010-10-27 2016-04-12 Capitalbio Corporation Luminophore-labeled molecules coupled with particles for microarray-based assays
AU2013221642A1 (en) * 2012-02-15 2014-09-25 Janssen Diagnostics, Llc Highly sensitive method for detecting low frequency mutations
CN103760355B (zh) 2013-12-05 2015-09-16 博奥生物集团有限公司 微阵列芯片检测中核苷酸序列的颗粒标记方法
WO2018152162A1 (fr) * 2017-02-15 2018-08-23 Omniome, Inc. Distinction des séquences par détection de dissociation de polymérase
CN110914454B (zh) * 2017-05-18 2023-07-14 华晶基因技术有限公司 应用全基因组捕获转座子间区段序列对受微生物污染的人类dna样本进行基因组序列分析
CN109371115A (zh) * 2018-08-24 2019-02-22 山东德诺生物科技有限公司 用于检测rs5918的引物探针组及其应用
WO2020068983A1 (fr) 2018-09-25 2020-04-02 Co-Diagnostics, Inc. Conception spécifique d'allèles d'amorces coopératives pour génotypage de variants d'acide nucléique amélioré
WO2024259297A2 (fr) * 2023-06-15 2024-12-19 Caris Science, Inc. Compositions et méthodes de ciblage d'adn extracellulaire

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530794A1 (fr) * 1991-09-06 1993-03-10 Roche Diagnostics GmbH Méthode pour la détection d'acides nucléiques ayant une séquence similaire
EP0593789A1 (fr) * 1992-05-08 1994-04-27 Sumitomo Metal Industries, Ltd. Methode de determination de la mutation pre-c du virus de l'hepatite b
WO1998028440A1 (fr) * 1996-12-23 1998-07-02 Pyrosequencing Ab Methode de sequençage d'adn sur la base de la detection d'une liberation de pyrophosphate
WO2000053812A2 (fr) * 1999-03-12 2000-09-14 President And Fellows Of Harvard College Amplification par replique de reseaux d'acides nucleiques
EP1061135A2 (fr) * 1999-06-17 2000-12-20 Becton Dickinson and Company Procédés et oligonucléotides pour la détection des variations des séquences d'acides nucléiques

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530794A1 (fr) * 1991-09-06 1993-03-10 Roche Diagnostics GmbH Méthode pour la détection d'acides nucléiques ayant une séquence similaire
EP0593789A1 (fr) * 1992-05-08 1994-04-27 Sumitomo Metal Industries, Ltd. Methode de determination de la mutation pre-c du virus de l'hepatite b
WO1998028440A1 (fr) * 1996-12-23 1998-07-02 Pyrosequencing Ab Methode de sequençage d'adn sur la base de la detection d'une liberation de pyrophosphate
WO2000053812A2 (fr) * 1999-03-12 2000-09-14 President And Fellows Of Harvard College Amplification par replique de reseaux d'acides nucleiques
EP1061135A2 (fr) * 1999-06-17 2000-12-20 Becton Dickinson and Company Procédés et oligonucléotides pour la détection des variations des séquences d'acides nucléiques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AHMADIAN A ET AL: "Genotyping by apyrase-mediated allele-specific extension.", NUCLEIC ACIDS RESEARCH. ENGLAND 15 DEC 2001, vol. 29, no. 24, 15 December 2001 (2001-12-15), pages E121, XP002236747, ISSN: 1362-4962 *
FAN J-B ET AL: "Parallel genotyping of human SNPs using generic high-density oligonucleotide tag arrays", GENOME RESEARCH 2000 UNITED STATES, vol. 10, no. 6, 2000, pages 853 - 860, XP002236784, ISSN: 1088-9051 *

Also Published As

Publication number Publication date
AU2002233540A1 (en) 2002-09-12
WO2002068684A2 (fr) 2002-09-06
AU2002233540A8 (en) 2008-02-14
WO2002068684A8 (fr) 2003-12-04

Similar Documents

Publication Publication Date Title
WO2003016565A3 (fr) Analyse de sequences d'adn
Ørum et al. Single base pair mutation analysis by PNA directed PCR clamping
US10093962B2 (en) Fast hybridization for next generation sequencing target enrichment
AU3064200A (en) A method of dna sequencing
WO2002068684A8 (fr) Méthode d'extension d'amorce spécifique d'allèle
WO2006023919A3 (fr) Detection des differences d'acide nucleique au moyen de clivage/ligase par endonuclease liberant des reactions, une electrophorese capillaire ou des micro-reseaux
EP1241266A4 (fr) Procede de detection de polymorphisme nucleotidique
WO2002083839A3 (fr) Analyse par biopuces de polymorphismes à simple nucléotide
WO2001075166A3 (fr) Compositions et methodes applicables a la detection et a la quantification d'une expression genique
WO1999005315A3 (fr) Analyse de sequences nucleotidiques
WO2001098528A3 (fr) Procede pour la mise en evidence de methylations de la cytosine
WO2002020836A3 (fr) Methode de sequençage de l'adn
WO2004092331A3 (fr) Composition et procede pour le sequençage d'acides nucleiques
EP2984183B1 (fr) Analyse génétique à base de nanosondes
WO2004020654A3 (fr) Dosages d'acide nucleique en rapport avec une phase solide, combinant une haute affinite avec une haute specificite
DE50112799D1 (de) Verfahren zum nachweis von cytosin-methylierungen
DK1620568T3 (da) Analyse til allelspecifik mutationspåvisning
US20020146723A1 (en) Candidate region mismatch scanning for genotyping and mutation detection
WO2002070736A8 (fr) Procede de detection de molecules d'acide nucleique
EP1300473B1 (fr) Procede de detection de polymorphismes nucleotidiques
Bazar et al. Mutation identification DNA analysis system (MIDAS) for detection of known mutations
CA2451394A1 (fr) Variation de la sequence du recepteur de l'il-4 associee aux diabetes de type 1
WO2003002721A3 (fr) Compositions et methodes de deduction d'une reponse a une statine
Okada et al. Genotyping of Thiopurine Methyltransferase Using PyrosequencingTM
DE50214183D1 (de) Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 36/2002 DUE TO A TECHNICAL PROBLEM AT THE TIME OF INTERNATIONAL PUBLICATION, SOME INFORMATION WAS MISSING (81). THE MISSING INFORMATION NOW APPEARS IN THE CORRECT VERSION.

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP