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WO2002064811A3 - Procede pour produire des proteines recombinantes, marquees par au moins un isotope - Google Patents

Procede pour produire des proteines recombinantes, marquees par au moins un isotope Download PDF

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Publication number
WO2002064811A3
WO2002064811A3 PCT/FR2002/000498 FR0200498W WO02064811A3 WO 2002064811 A3 WO2002064811 A3 WO 2002064811A3 FR 0200498 W FR0200498 W FR 0200498W WO 02064811 A3 WO02064811 A3 WO 02064811A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
cells
micro
recombinant proteins
isotope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2002/000498
Other languages
English (en)
Other versions
WO2002064811A2 (fr
Inventor
Dominique Desplancq
Bruno Kieffer
Karsten Schmidt
Etienne Weiss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Strasbourg
Original Assignee
Universite de Strasbourg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Strasbourg filed Critical Universite de Strasbourg
Publication of WO2002064811A2 publication Critical patent/WO2002064811A2/fr
Publication of WO2002064811A3 publication Critical patent/WO2002064811A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention se rapporte à un procédé pour produire des protéines recombinantes, marquées de façon uniforme et complète par au moins un isotope, à partir de cellules ou de microorganismes recombinants autotrophes et/ou phototrophes. Ce procédé consiste à mettre en culture les cellules ou microorganismes dans un milieu comprenant au moins un isotope stable, à isoler ces microorganismes/cellules, puis à purifier les protéines recombinantes à partir des microorganismes, de cellules ou du milieu de culture. Les cellules/microorganismes sont mis en culture en aérobie, en anaérobie ou en fermentation; la culture en aérobie s'effectuant en présence de CO2 atmosphérique, celle en anaérobie et en fermentation sans CO2 atmosphérique. Les cultures en anaérobie et en fermentation s'effectuent en régulant le pH, l'intensité d'une source de lumière et la durée d'exploitation en continu à cette source.
PCT/FR2002/000498 2001-02-09 2002-02-08 Procede pour produire des proteines recombinantes, marquees par au moins un isotope Ceased WO2002064811A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0101790A FR2820758B1 (fr) 2001-02-09 2001-02-09 Procede pour produire des proteines recombinantes, marquees par au moins un isotope
FR01/01790 2001-02-09

Publications (2)

Publication Number Publication Date
WO2002064811A2 WO2002064811A2 (fr) 2002-08-22
WO2002064811A3 true WO2002064811A3 (fr) 2003-03-20

Family

ID=8859840

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/000498 Ceased WO2002064811A2 (fr) 2001-02-09 2002-02-08 Procede pour produire des proteines recombinantes, marquees par au moins un isotope

Country Status (2)

Country Link
FR (1) FR2820758B1 (fr)
WO (1) WO2002064811A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2004029254A1 (ja) * 2002-09-30 2006-01-26 味の素株式会社 安定同位体標識タンパク質の製造方法
AT501629B1 (de) * 2005-04-05 2007-10-15 Erber Ag Herstellung von hochgradig isotopenmarkierten, sekundären, mikrobiellen stoffwechselprodukten sowie stoffwechselprodukte
CN104330568A (zh) * 2014-10-15 2015-02-04 南京医科大学 检测蓝藻毒素的胶体金免疫层析试剂盒

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2206881A (en) * 1987-07-01 1989-01-18 Univ Dundee Production of labelled organic compounds
FR2637293A1 (fr) * 1988-07-26 1990-04-06 Commissariat Energie Atomique Plasmides recombinants, vecteurs comprenant lesdits plasmides, leurs procedes de production, souche de cyanobacterie synechocystis modifiee, apte a etre hote desdits plasmides et/ou desdits vecteurs
SU1555354A1 (ru) * 1987-09-18 1990-04-07 Институт Физиологии Растений Им.К.А.Тимирязева Ан Ссср Способ получени меченых соединений
SU1806184A3 (ru) * 1990-09-19 1993-03-30 Иhctиtуt Физиoлoгии Pactehий Pah Cпocoб kульtиbиpobahия mиkpoboдopocлeй
WO1997018321A1 (fr) * 1995-11-15 1997-05-22 Martek Corporation Production photosynthetique de proteines de recombinaison marqueespar des isotopes stables
DE19839491A1 (de) * 1998-08-29 2000-03-02 Hermann Heumann Verfahren zur Markierung von Biopolymeren mit Isotopen

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2206881A (en) * 1987-07-01 1989-01-18 Univ Dundee Production of labelled organic compounds
SU1555354A1 (ru) * 1987-09-18 1990-04-07 Институт Физиологии Растений Им.К.А.Тимирязева Ан Ссср Способ получени меченых соединений
FR2637293A1 (fr) * 1988-07-26 1990-04-06 Commissariat Energie Atomique Plasmides recombinants, vecteurs comprenant lesdits plasmides, leurs procedes de production, souche de cyanobacterie synechocystis modifiee, apte a etre hote desdits plasmides et/ou desdits vecteurs
SU1806184A3 (ru) * 1990-09-19 1993-03-30 Иhctиtуt Физиoлoгии Pactehий Pah Cпocoб kульtиbиpobahия mиkpoboдopocлeй
WO1997018321A1 (fr) * 1995-11-15 1997-05-22 Martek Corporation Production photosynthetique de proteines de recombinaison marqueespar des isotopes stables
DE19839491A1 (de) * 1998-08-29 2000-03-02 Hermann Heumann Verfahren zur Markierung von Biopolymeren mit Isotopen

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BEHRENS PAUL W ET AL: "Microalgae as a source of stable isotopically labeled compounds.", JOURNAL OF APPLIED PHYCOLOGY, vol. 6, no. 2, 1994, pages 113 - 121, XP001030623, ISSN: 0921-8971 *
BELENKY MARINA ET AL: "Monitoring of isotope substitution in cyanobacteria by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.", ANALYTICAL BIOCHEMISTRY, vol. 280, no. 1, 10 April 2000 (2000-04-10), pages 182 - 185, XP002180461, ISSN: 0003-2697 *
COX J ET AL: "STABLE-ISOTOPE-LABELED BIOCHEMICALS FROM MICROALGAE", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 6, 1988, pages 279 - 282, XP002026962, ISSN: 0167-7799 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1988, WOLK C P ET AL: "ISOLATION AND COMPLEMENTATION OF MUTANTS OF ANABAENA-SP STRAIN PCC 7120 UNABLE TO GROW AEROBICALLY ON DINITROGEN", XP002180464, Database accession no. PREV198885100775 *
DATABASE WPI Section Ch Week 199044, Derwent World Patents Index; Class D16, AN 1990-333421, XP002225856 *
DATABASE WPI Section Ch Week 199423, Derwent World Patents Index; Class D16, AN 1994-190566, XP002225855 *
DESPLANCQ D; KIEFFER B; SCHMIDT K; POSTEN C; FORSTER A; OUDET P; STRUB J M; VAN DORSSELAER A; WEISS E: "Cost-Effective and Uniform (13)C- and (15)N-Labeling of the 24-kDa N-Terminal Domain of the Escherichia coli Gyrase B by Overexpression in the Photoautotrophic Cyanobacterium Anabaena sp. PCC 7120.", PROTEIN EXPRESSION AND PURIFICATION, vol. 23, no. 1, October 2001 (2001-10-01), pages 207 - 17, XP002180463 *
JOURNAL OF BACTERIOLOGY, vol. 170, no. 3, 1988, pages 1239 - 1244, ISSN: 0021-9193 *
SAILER MILOSLAV ET AL: "Nitrogen-15 and carbon-13 labeled media from Anabaena sp. for universal isotopic labeling of bacteriocins: NMR resonance assignments of leucocin A from Leuconostoc gelidum and nisin A from Lactococcus lactis.", BIOCHEMISTRY, vol. 32, no. 1, 1993, pages 310 - 318, XP001030560, ISSN: 0006-2960 *
TOVEY K C ET AL: "A NEW METHOD FOR THE PREPARATION OF UNIFORMLY CARBON-14 LABELED COMPOUNDS BY USING ANACYSTIS-NIDULANS", BIOCHEMICAL JOURNAL, vol. 142, no. 1, 1974, pages 47 - 56, XP001030598, ISSN: 0264-6021 *

Also Published As

Publication number Publication date
WO2002064811A2 (fr) 2002-08-22
FR2820758A1 (fr) 2002-08-16
FR2820758B1 (fr) 2004-05-14

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