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WO2002064750A3 - Expression system for seed proteins - Google Patents

Expression system for seed proteins Download PDF

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Publication number
WO2002064750A3
WO2002064750A3 PCT/US2002/004909 US0204909W WO02064750A3 WO 2002064750 A3 WO2002064750 A3 WO 2002064750A3 US 0204909 W US0204909 W US 0204909W WO 02064750 A3 WO02064750 A3 WO 02064750A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression system
seed proteins
compositions
grains
directed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/004909
Other languages
French (fr)
Other versions
WO2002064750A2 (en
Inventor
Ning Huang
Daichang Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Invitria Inc
Original Assignee
Ventria Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/847,232 external-priority patent/US20030172403A1/en
Application filed by Ventria Bioscience Inc filed Critical Ventria Bioscience Inc
Priority to AU2002244071A priority Critical patent/AU2002244071A1/en
Publication of WO2002064750A2 publication Critical patent/WO2002064750A2/en
Publication of WO2002064750A3 publication Critical patent/WO2002064750A3/en
Priority to US10/639,779 priority patent/US20040078851A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/20—Malt products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Toxicology (AREA)
  • Animal Husbandry (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physiology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention is directed to methods and compositions for high level expression of heterologous polypeptides in the grains of transgenic plants and compositions resulting therefrom that are suitable for oral delivery or other uses.
PCT/US2002/004909 2000-05-02 2002-02-14 Expression system for seed proteins Ceased WO2002064750A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002244071A AU2002244071A1 (en) 2001-02-14 2002-02-14 Expression system for seed proteins
US10/639,779 US20040078851A1 (en) 2000-05-02 2003-08-13 Production of human growth factors in monocot seeds

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US26919901P 2001-02-14 2001-02-14
US26918801P 2001-02-14 2001-02-14
US60/269,188 2001-02-14
US60/269,199 2001-02-14
US09/847,232 2001-05-02
US09/847,232 US20030172403A1 (en) 2000-05-02 2001-05-02 Plant transcription factors and enhanced gene expression

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/639,779 Continuation-In-Part US20040078851A1 (en) 2000-05-02 2003-08-13 Production of human growth factors in monocot seeds

Publications (2)

Publication Number Publication Date
WO2002064750A2 WO2002064750A2 (en) 2002-08-22
WO2002064750A3 true WO2002064750A3 (en) 2002-11-21

Family

ID=27402152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/004909 Ceased WO2002064750A2 (en) 2000-05-02 2002-02-14 Expression system for seed proteins

Country Status (2)

Country Link
AU (1) AU2002244071A1 (en)
WO (1) WO2002064750A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US7304208B2 (en) 2000-05-02 2007-12-04 Ventria Bioscience Expression of human serum albumin (HSA) in monocot seeds
GB2377446B (en) 2001-07-13 2005-06-29 Lars Erik Berglund Expression of cobalamin binding proteins in transgenic plants
WO2004099405A1 (en) * 2003-04-11 2004-11-18 Ventria Bioscience Human blood proteins expressed in monocot seeds
CA2548749A1 (en) * 2003-12-09 2005-06-23 Ventria Bioscience High-level expression of fusion polypeptides in plant seeds utilizing seed-storage proteins as fusion carriers
WO2005067699A1 (en) * 2003-12-23 2005-07-28 Ventria Bioscience Methods of expressing heterologous protein in plant seeds using monocot non seed-storage protein promoters
JP4581098B2 (en) * 2005-03-07 2010-11-17 国立大学法人名古屋大学 Methods for expressing and accumulating peptides in plants
US7550579B2 (en) 2005-04-29 2009-06-23 Pioneer Hi-Bred International, Inc. Pericarp-preferred regulatory element
ITUD20080055A1 (en) * 2008-03-13 2009-09-14 Transactiva S R L PROCEDURE FOR THE PRODUCTION OF A HUMAN PROTEIN ON THE PLANT, IN PARTICULAR A RECOMBINANT HUMAN LYSOSOMIAL ENZYME IN CEREALS ENDOSPERMA
US20100313307A1 (en) * 2008-06-28 2010-12-09 Donald Danforth Plant Science Center Protein production and storage in plants
CN103575903B (en) * 2012-08-07 2016-01-20 中国检验检疫科学研究院 A kind of human lactoferrin sandwich ELISA detection kit and application thereof
CN102994514B (en) * 2012-11-07 2015-06-10 武汉禾元生物科技股份有限公司 Method for producing, separating and purifying recombinant human antitrypsin (OsrAAT) from rice seeds
US12295990B2 (en) 2018-11-09 2025-05-13 The Wistar Institute Of Anatomy And Biology Use of lactoferrin for generating myeloid-derived suppressor cells
EP4222167A1 (en) 2020-09-30 2023-08-09 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5543576A (en) * 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5543576A (en) * 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HORVATH ET AL.: "The production of recombinant proteins in transgenic Barley grains", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 4, 15 February 2000 (2000-02-15), pages 1914 - 1919, XP002173225 *
WU ET AL.: "The GCN4 motif in a rice glutelin gene is essential for endosperm-specific gene expression and is activated by opaque-2 in transgenic rice plants", THE PLANT JOURNAL, vol. 14, no. 6, 1998, pages 673 - 683, XP001079181 *
ZHENG ET AL.: "A distal promoter region of the rice seed storage protein glutelin gene enhanced quantitative gene expression", PLANT SCIENCE, vol. 128, 1997, pages 59 - 65, XP002955709 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants

Also Published As

Publication number Publication date
WO2002064750A2 (en) 2002-08-22
AU2002244071A1 (en) 2002-08-28

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