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WO2002064743A3 - Confirming the exon content of rna transcripts by pcr using primers complementary to each respective exon - Google Patents

Confirming the exon content of rna transcripts by pcr using primers complementary to each respective exon Download PDF

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Publication number
WO2002064743A3
WO2002064743A3 PCT/US2002/004219 US0204219W WO02064743A3 WO 2002064743 A3 WO2002064743 A3 WO 2002064743A3 US 0204219 W US0204219 W US 0204219W WO 02064743 A3 WO02064743 A3 WO 02064743A3
Authority
WO
WIPO (PCT)
Prior art keywords
exon
pcr
confirming
rna transcripts
content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/004219
Other languages
French (fr)
Other versions
WO2002064743A2 (en
Inventor
Peter S Linsley
Matthew Biery
Mao Mao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rosetta Inpharmatics LLC
Original Assignee
Rosetta Inpharmatics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rosetta Inpharmatics LLC filed Critical Rosetta Inpharmatics LLC
Priority to AU2002250066A priority Critical patent/AU2002250066A1/en
Publication of WO2002064743A2 publication Critical patent/WO2002064743A2/en
Publication of WO2002064743A3 publication Critical patent/WO2002064743A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to methods and systems (e.g., computer systems and computer program products) for identifying and characterizing genes using microarrays. In particular, the invention provides for improved, robust methods for detecting genes through the use of microarrays to analyze the expression state of the genome. Genes which are expressed can be mapped to their respective positions in the genome, and the structure of such genes can be determined.
PCT/US2002/004219 2001-02-12 2002-02-12 Confirming the exon content of rna transcripts by pcr using primers complementary to each respective exon Ceased WO2002064743A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002250066A AU2002250066A1 (en) 2001-02-12 2002-02-12 Confirming the exon content of rna transcripts by pcr using primers complementary to each respective exon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26817301P 2001-02-12 2001-02-12
US60/268,173 2001-02-12

Publications (2)

Publication Number Publication Date
WO2002064743A2 WO2002064743A2 (en) 2002-08-22
WO2002064743A3 true WO2002064743A3 (en) 2002-10-31

Family

ID=23021791

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/004219 Ceased WO2002064743A2 (en) 2001-02-12 2002-02-12 Confirming the exon content of rna transcripts by pcr using primers complementary to each respective exon

Country Status (2)

Country Link
AU (1) AU2002250066A1 (en)
WO (1) WO2002064743A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1609872A1 (en) * 2004-06-25 2005-12-28 Stichting Voor De Technische Wetenschappen A method for selecting a gene involved in nisin resistance in bacteria
JP4772816B2 (en) * 2008-03-21 2011-09-14 株式会社東芝 Design method of microarray and negative control probe
CN110706738B (en) * 2019-10-30 2020-11-20 腾讯科技（深圳）有限公司 Protein structure information prediction method, device, equipment and storage medium
CN120290752B (en) * 2025-06-13 2025-09-16 北京大学成都前沿交叉生物技术研究院 Capture probe, kit comprising same and use of same in detection of T cell and B cell immune repertoire at spatial resolution

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618702A (en) * 1988-08-19 1997-04-08 City Of Hope PCR amplification of mRNA
US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618702A (en) * 1988-08-19 1997-04-08 City Of Hope PCR amplification of mRNA
US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

Also Published As

Publication number Publication date
WO2002064743A2 (en) 2002-08-22
AU2002250066A1 (en) 2002-08-28

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