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WO2002061106A3 - Verfahren zur fermentativen herstellung von cystein, cystin und glutathion - Google Patents

Verfahren zur fermentativen herstellung von cystein, cystin und glutathion Download PDF

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Publication number
WO2002061106A3
WO2002061106A3 PCT/EP2002/001122 EP0201122W WO02061106A3 WO 2002061106 A3 WO2002061106 A3 WO 2002061106A3 EP 0201122 W EP0201122 W EP 0201122W WO 02061106 A3 WO02061106 A3 WO 02061106A3
Authority
WO
WIPO (PCT)
Prior art keywords
cystine
glutathione
fermentation
cysteine
sat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/001122
Other languages
English (en)
French (fr)
Other versions
WO2002061106A2 (de
Inventor
Markus Wirtz
Ruediger Hell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Original Assignee
Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut fuer Pflanzengenetik und Kulturpflanzenforschung filed Critical Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Priority to AU2002244700A priority Critical patent/AU2002244700A1/en
Publication of WO2002061106A2 publication Critical patent/WO2002061106A2/de
Publication of WO2002061106A3 publication Critical patent/WO2002061106A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Auffindung von Genen und DNA-Sequenzen, die für das Enzym Serin-Acetyltransferase (SAT) kodieren und für die fermentative Herstellung von Cystein geeignet sind. Weiter betrifft die Erfindung ein Verfahren zur Herstellung schwefelhaltiger Verbindungen wie Cystein, Cystin und Glutathion in Bakterien und anderen Wirtsorganismen mittels Überexpression von SAT-Genen, wobei der Wirtsorganismus in seiner eigenen Glutathionsynthese gestört ist.
PCT/EP2002/001122 2001-02-02 2002-02-04 Verfahren zur fermentativen herstellung von cystein, cystin und glutathion Ceased WO2002061106A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002244700A AU2002244700A1 (en) 2001-02-02 2002-02-04 Method for producing cysteine, cystine and glutathione by fermentation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10104722A DE10104722A1 (de) 2001-02-02 2001-02-02 Verfahren zur fermentativen Herstellung von Cystein, Cystin und Glutathion
DE10104722.3 2001-02-02

Publications (2)

Publication Number Publication Date
WO2002061106A2 WO2002061106A2 (de) 2002-08-08
WO2002061106A3 true WO2002061106A3 (de) 2003-03-13

Family

ID=7672632

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/001122 Ceased WO2002061106A2 (de) 2001-02-02 2002-02-04 Verfahren zur fermentativen herstellung von cystein, cystin und glutathion

Country Status (3)

Country Link
AU (1) AU2002244700A1 (de)
DE (1) DE10104722A1 (de)
WO (1) WO2002061106A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10237479A1 (de) * 2002-08-16 2004-02-26 Degussa Ag Schwefel-haltiges Tierfuttermitteladditiv
US7348037B2 (en) 2002-08-16 2008-03-25 Evonik Degussa Gmbh Sulfur-containing animal-feed additives
JP2006517796A (ja) 2003-02-18 2006-08-03 メタボリック エクスプローラー 代謝経路の生成または改変を可能とする進化した微生物の生産方法
KR20190097218A (ko) 2016-12-22 2019-08-20 디에스엠 아이피 어셋츠 비.브이. 효소에 의한 시스틴의 환원

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19539952A1 (de) * 1995-10-26 1997-04-30 Consortium Elektrochem Ind Verfahren zur Herstellung von O-Acetylserin, L-Cystein und L-Cystein-verwandten Produkten

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HELL R: "MOLECULAR PHYSIOLOGY OF PLANT SULFUR METABOLISM", PLANTA, SPRINGER VERLAG, DE, vol. 202, 1997, pages 138 - 148, XP000856032, ISSN: 0032-0935 *
HOWARTH ET AL: "cysteine biosynthesis in higher plants;a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1350, 1997, pages 123 - 127, XP002115632, ISSN: 0167-4781 *
NAKAMORI ET AL: "overproduction of L-cysteine and L-cystine by Escherichia coli strains with a genetically altered serine acetyltransferase", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 64, no. 5, May 1998 (1998-05-01), pages 1607 - 1611, XP002115630, ISSN: 0099-2240 *
NOJI ET AL: "ISOFORM-DEPENDENT DIFFERENCES IN FEEDBACK REGULATION AND SUBCELLULAR LOCALIZATION OF SERINE ACETYLTRANSFERASE INVOLVED IN CYSTEINE BIOSYNTHESIS FROM ARABIDOPSIS THALIANA", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 49, 4 December 1998 (1998-12-04), pages 32739 - 32745, XP002115629, ISSN: 0021-9258 *
TAKAGI HIROSHI ET AL: "Overproduction of L-cysteine and L-cystine by expression of genes for feedback inhibition-insensitive serine acetyltransferase from Arabidopsis thaliana in Escherichia coli.", FEMS MICROBIOLOGY LETTERS, vol. 179, no. 2, 15 October 1999 (1999-10-15), pages 453 - 459, XP002209321, ISSN: 0378-1097 *

Also Published As

Publication number Publication date
WO2002061106A2 (de) 2002-08-08
AU2002244700A1 (en) 2002-08-12
DE10104722A1 (de) 2002-08-14

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