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WO2002057665A2 - Systeme d'etancheite pour plaque a puits - Google Patents

Systeme d'etancheite pour plaque a puits Download PDF

Info

Publication number
WO2002057665A2
WO2002057665A2 PCT/US2002/000593 US0200593W WO02057665A2 WO 2002057665 A2 WO2002057665 A2 WO 2002057665A2 US 0200593 W US0200593 W US 0200593W WO 02057665 A2 WO02057665 A2 WO 02057665A2
Authority
WO
WIPO (PCT)
Prior art keywords
well
cups
tip
sealably engaging
opening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/000593
Other languages
English (en)
Other versions
WO2002057665A3 (fr
Inventor
Michael J. Berna
Robert L. Grenz
Roger Q. Roberts
Roger R. Rohrdanz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Ansys Technologies Inc
Original Assignee
Eli Lilly and Co
Ansys Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co, Ansys Technologies Inc filed Critical Eli Lilly and Co
Publication of WO2002057665A2 publication Critical patent/WO2002057665A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002057665A3 publication Critical patent/WO2002057665A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption

Definitions

  • the present invention generally relates to multi-well plate and column arrays for use in the analysis of liquid and is more particularly directed to seals for such multi-well plates.
  • a variety of multi-well arrangements are used in the analysis or assay for a great number of a small quantity of liquids.
  • a plate having 96 wells is a popular configuration. Plates of this type may include a separation medium for removing solid or particulate matter from a liquid by filtration or a chromatographic medium for selectively separating, or indicating, a particular characteristic of a fluid being assayed.
  • a typical prior art solid phase extraction assembly is described in U.S. Patent No. 5,906,796 to Blevins and Schultheis.
  • the multi-well plate described in the referenced patent typically includes 96 wells arranged in rows and columns in which a solid phase extraction medium is placed and sequentially treated with liquid reagents and washes involved in an assay of interest .
  • this type of multi-well plate has dimensions in the order of 3 inches by 5 inches, and accordingly, a 96 well, or compartment, assay plate has a very small compartment dimension. Allowing for supporting well structure, in a typical 96 well plate, having wells arranged in a typical 8 x 12 configuration will have well diameters in the order of 0.3 inches .
  • the present invention provides a seal for a multi-well plate having a plurality of wells with each well having an opening on one end thereof for influxing a fluid and a type in another end thereof for effluxing the fluid.
  • a seal for a multi-well plate in accordance with the present invention generally includes a planar member having a top and a bottom.
  • a plurality of recesses are formed in the top of the member with the plurality of recesses being uniformly spaced apart from one another and each recess being sized for sealably engaging a corresponding well tip.
  • a plurality of cups are formed in the bottom of the member and sized for sealably engaging a corresponding well opening in order to enable the member to effect a sealed engagement with all of the well openings or all of the well tips.
  • Thin wall portions may be formed in the planar member between rows of cups for enabling the planar member to be divided along a thin wall portion.
  • the planar member may be reduced in size in order to accommodate, or match, the number of wells actually used in a 96 well plate or other format.
  • each of the recesses may include a bottom for sealably engaging the corresponding tip and a tapered sidewall for guiding the corresponding tip into the recess bottom.
  • multi-well plates of, for example 96 wells may be rapidly and easily sealed individually by the facilitation of the tapered sidewalls in each of the recesses.
  • each of the plurality of cups include a body portion for sealably engaging the corresponding well opening and a tapered portion for guiding the corresponding well opening into the body portion. This enables rapid alignment of the member body portions with the well opening in a manner similar to the tapered sidewall function hereinabove described.
  • each of the plurality of cups may be axially aligned with one of the plurality of recesses in order to enable a plurality of well plates to be stacked vertically.
  • a sealing system in accordance with the present invention for a plurality of well plates includes a plurality of seals with each seal including a planar member having a top and a bottom and plurality of recesses formed in the member top which are uniformly spaced apart from one another and sized for sealably engaging a corresponding well tip.
  • a plurality of cups formed in the member bottom are provided and sized for sealably engaging a corresponding well opening in order to enable one of the plurality of seals to sealably engage all of the well opening of one plate and all of the well tips of another well plate.
  • seal in accordance with the present invention may be used to seal either all of the well openings or all of the well tips or well plate, it should be appreciated that the inventory of such seals is significantly reduced and the necessity for selecting a coordinated seal dedicated to sealing either the well opening or the well tips is eliminated.
  • Figure 1 is a perspective view of two seals in accordance with the present invention positioned with respect to a multi-well plate in order to engage all of the wells of the plate and all of the tips of the plate;
  • Figure 2 is a side view of a plurality of seals which form a system for sealing a plurality of multi-well plates in a stacked configuration
  • Figure 3 is a cross-sectional view taken along the line 3-3 of Figure 2 showing a sealed engagement between the seal and multi-well plates;
  • Figure 4 is a perspective illustration of a seal , made in accordance with the present invention, being divided into two smaller seals.
  • seal 10 and sealing system 12 in accordance with the present invention for a plurality of multi-well plates 16.
  • Each of the plates 16 include a plurality of wells 20, for example 96 wells 20, with each well 20 having an opening 22 in one end 24 for influxing a fluid and a tip 28 in another end 30 of the well 20 for effluxing the fluid (not shown) .
  • the seal system 12 includes a plurality of seals 10 with each of the seals 10 including a planar member 34 having a top 36 and a bottom 38.
  • the member 34 may be formed from any suitable material such as, for example, by any conventional molding process.
  • a plurality of recess 42 are formed in the member top 28 with the plurality of recess 42 being uniformly spaced apart from one another and each recess being sized for sealably engaging one of the well tips 28.
  • a plurality of cups 44 are formed in the member bottom 30 and sized for sealing one of the well openings 22. Because the member 34 includes the plurality of recesses 42 formed in the top 36 and a plurality of cups 44 are formed in the bottom 38, the member 34 can effect a sealed engagement with all of the well openings 22 or, alternatively, all of the well tips 28. Thus, the seal 10 may be utilized to seal either one side 48 of the plate 16 with well openings 22 or another side 50 with the tips 28 extending therefrom.
  • the sealing system 12 in accordance with the present invention which includes a plurality of seals 10 enables a plurality of well plates 16 to be sealably stacked for storage either before or after use.
  • Each of the recesses 42 includes a bottom 56 for sealably engaging a tip 28 and a tapered sidewall 58 for aligning and guiding the 28 into the recess bottom 56.
  • this greatly facilitates the coupling of all of the recesses 42 with all of the tips 28 in a simultaneous manner.
  • the difficulty in alignment of 96 separate tips 28 with 96 separate recesses is greatly facilitated by the tapered sidewalls 58.
  • each of the cups 44 is formed with a body portion 62 for sealably engaging a well opening 22 and a tapered portion 64 for aligning and guiding the corresponding well opening 22 onto the body portion 62. This correspondingly facilitates the alignment and sealing of 96 well openings 22 by 96 cup body portions 62.
  • a thin wall portion 100 is formed between cups 44 for enabling, or facilitating, division of seal 10, planar member 34 into two smaller seals 102, 104. This may be done manually and enables the seal 10 to be utilized in sealing smaller multi-well plates, not shown.
  • the seal 10 may have 96 cups 44 and recesses 42 and may be divided into two seals 102, 104 for using 48 wells of the 96 well plate .
  • the ability to tear the plate seal 10 into smaller portions 102, 104 allows a user to use and seal a portion of the 96 well plate 16.
  • a smaller portion 102, 104 may be used to seal a plate having only 8 columns (called octacolumns) (not shown) and 24 well plates (3 rows of 8) (not shown) .
  • Other formats include well plates of 384 and 1536 wells.
  • the larger plates may be closed with multiple seals 10 in accordance with the present invention.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

L'invention concerne un système d'étanchéité pour plaque à puits comportant une pluralité de puits dont chacun présente une ouverture à une extrémité pour permettre l'afflux d'un liquide et à une autre extrémité pour la sortie dudit liquide. Le système d'étanchéité comprend un élément plan comportant un dessus et une base et une pluralité de cavités formées au niveau du dessus et une pluralité de coupelles formées dans la base. Une pluralité de cavités sont espacées de manière régulière les unes des autres et chacune est dimensionnée de sorte à entrer de manière étanche dans l'extrémité correspondante d'un puits. La pluralité de coupelles sont espacées régulièrement les unes des autres et sont dimensionnées de sorte à entrer de manière étanche dans une ouverture correspondante de puits afin de permettre à l'élément d'entrer de manière étanche dans toutes les ouvertures de puits ou toutes les extrémités de puits.
PCT/US2002/000593 2001-01-16 2002-01-09 Systeme d'etancheite pour plaque a puits Ceased WO2002057665A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US76453301A 2001-01-16 2001-01-16
US09/764,533 2001-01-16
US09/849,046 2001-05-04
US09/849,046 US20020093147A1 (en) 2001-01-16 2001-05-04 Well plate seal

Publications (2)

Publication Number Publication Date
WO2002057665A2 true WO2002057665A2 (fr) 2002-07-25
WO2002057665A3 WO2002057665A3 (fr) 2004-05-27

Family

ID=27117480

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000593 Ceased WO2002057665A2 (fr) 2001-01-16 2002-01-09 Systeme d'etancheite pour plaque a puits

Country Status (2)

Country Link
US (1) US20020093147A1 (fr)
WO (1) WO2002057665A2 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004045054A1 (de) * 2004-09-15 2006-03-30 Eppendorf Ag Vorrichtung zur saugdichten Abdeckung einer Filtereinrichtung
DE10321042B4 (de) * 2003-01-17 2006-09-21 Greiner Bio-One Gmbh Biochip-Träger
WO2007046743A1 (fr) * 2005-10-18 2007-04-26 Ge Healthcare Bio-Sciences Ab Plaque multipuits
US7737331B2 (en) 2004-02-25 2010-06-15 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
US8007744B2 (en) 2003-01-17 2011-08-30 Greiner Bio-One Gmbh Sample container for analyses
US9005549B2 (en) 2003-01-17 2015-04-14 Greiner Bio-One Gmbh High throughput polymer-based microarray slide

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060228265A1 (en) * 2005-04-07 2006-10-12 Peng Sean X Particulate separation filters and methods
EP3033276B1 (fr) * 2013-08-16 2019-03-06 VANRX Pharmasystems Inc. Procédé de remplissage de contenants pharmaceutiques
EP3646950B1 (fr) 2018-10-31 2023-08-23 SCHOTT Pharma Schweiz AG Unité de transport et structure d'emballage pour le transport et le stockage d'une pluralité de contenants pour substances à usage pharmaceutique, médical ou cosmétique
AT522580B1 (de) * 2019-10-24 2020-12-15 Tupack Verpackungen Ges M B H Zwischenlage für die Anordnung zwischen aufeinandergestapelten Verpackungsschachteln

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824641A (en) * 1986-06-20 1989-04-25 Cetus Corporation Carousel and tip
US4895706A (en) * 1986-10-28 1990-01-23 Costar Corporation Multi-well filter strip and composite assemblies
US5180555A (en) * 1988-02-16 1993-01-19 Bio Merieux Microbiological analysis cup or the like
US5342581A (en) * 1993-04-19 1994-08-30 Sanadi Ashok R Apparatus for preventing cross-contamination of multi-well test plates
US5441895A (en) * 1993-12-07 1995-08-15 Jakubowicz; Raymond F. Reagent cup shape allowing stacking without dislodging reagent
AU9786798A (en) * 1997-10-10 1999-05-03 Biosepra Inc. Aligned multiwell multiplate stack and method for processing biological/chemicalsamples using the same
US6096562A (en) * 1997-10-27 2000-08-01 Nalge Nunc International Corporation Multi-slide assembly including slide, frame and strip cap, and methods thereof
US6159368A (en) * 1998-10-29 2000-12-12 The Perkin-Elmer Corporation Multi-well microfiltration apparatus

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10321042B4 (de) * 2003-01-17 2006-09-21 Greiner Bio-One Gmbh Biochip-Träger
US8007744B2 (en) 2003-01-17 2011-08-30 Greiner Bio-One Gmbh Sample container for analyses
US9005549B2 (en) 2003-01-17 2015-04-14 Greiner Bio-One Gmbh High throughput polymer-based microarray slide
US7737331B2 (en) 2004-02-25 2010-06-15 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
US7737117B2 (en) 2004-02-25 2010-06-15 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
US7943737B2 (en) 2004-02-25 2011-05-17 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
US7943734B2 (en) 2004-02-25 2011-05-17 Pioneer International, Inc. Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
US7943735B2 (en) 2004-02-25 2011-05-17 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis crystal polypetides, polynucleotides, and compositions thereof
US7943736B2 (en) 2004-02-25 2011-05-17 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
DE102004045054A1 (de) * 2004-09-15 2006-03-30 Eppendorf Ag Vorrichtung zur saugdichten Abdeckung einer Filtereinrichtung
WO2007046743A1 (fr) * 2005-10-18 2007-04-26 Ge Healthcare Bio-Sciences Ab Plaque multipuits
CN101291737B (zh) * 2005-10-18 2010-09-08 通用电气健康护理生物科学股份公司 多孔板

Also Published As

Publication number Publication date
WO2002057665A3 (fr) 2004-05-27
US20020093147A1 (en) 2002-07-18

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