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WO2002055539A1 - Procede de concentration de proteines insolubles par filtrage a membrane vibrante - Google Patents

Procede de concentration de proteines insolubles par filtrage a membrane vibrante Download PDF

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Publication number
WO2002055539A1
WO2002055539A1 PCT/EP2002/000063 EP0200063W WO02055539A1 WO 2002055539 A1 WO2002055539 A1 WO 2002055539A1 EP 0200063 W EP0200063 W EP 0200063W WO 02055539 A1 WO02055539 A1 WO 02055539A1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
membrane
proteins
extract
insoluble
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/000063
Other languages
English (en)
Inventor
Benoit Champluvier
Philippe Jean Gervais Ghislain Permanne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlaxoSmithKline Biologicals SA
Original Assignee
GlaxoSmithKline Biologicals SA
SmithKline Beecham Biologicals SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GlaxoSmithKline Biologicals SA, SmithKline Beecham Biologicals SA filed Critical GlaxoSmithKline Biologicals SA
Priority to EP02704642A priority Critical patent/EP1349868B1/fr
Priority to DE60201496T priority patent/DE60201496T2/de
Priority to CA002433689A priority patent/CA2433689A1/fr
Priority to AT02704642T priority patent/ATE278706T1/de
Priority to JP2002556607A priority patent/JP4074519B2/ja
Priority to US10/250,818 priority patent/US6939697B2/en
Publication of WO2002055539A1 publication Critical patent/WO2002055539A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/147—Microfiltration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/16—Feed pretreatment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/16—Rotary, reciprocated or vibrated modules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/04—Specific process operations in the feed stream; Feed pretreatment

Landscapes

  • Chemical & Material Sciences (AREA)
  • Water Supply & Treatment (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Glass Compositions (AREA)
  • Iron Core Of Rotating Electric Machines (AREA)

Abstract

Cette invention se rapporte à un procédé de purification de protéines, qui consiste à appliquer des extraits de protéines à un filtre à membrane vibrante pourvu d'une membrane hydrophile.
PCT/EP2002/000063 2001-01-09 2002-01-07 Procede de concentration de proteines insolubles par filtrage a membrane vibrante Ceased WO2002055539A1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP02704642A EP1349868B1 (fr) 2001-01-09 2002-01-07 Procede de concentration de proteines insolubles par filtrage a membrane vibrante
DE60201496T DE60201496T2 (de) 2001-01-09 2002-01-07 Verfahren zur konzentrierung von unlöslichen protein mittels filtration mit schwingenden membranen
CA002433689A CA2433689A1 (fr) 2001-01-09 2002-01-07 Procede de concentration de proteines insolubles par filtrage a membrane vibrante
AT02704642T ATE278706T1 (de) 2001-01-09 2002-01-07 Verfahren zur konzentrierung von unlöslichen protein mittels filtration mit schwingenden membranen
JP2002556607A JP4074519B2 (ja) 2001-01-09 2002-01-07 振動膜濾過により不溶タンパク質を濃縮する方法
US10/250,818 US6939697B2 (en) 2001-01-09 2002-01-07 Process to concentrate insoluble proteins by vibrating membrane filtration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0100513.1 2001-01-09
GBGB0100513.1A GB0100513D0 (en) 2001-01-09 2001-01-09 Process

Publications (1)

Publication Number Publication Date
WO2002055539A1 true WO2002055539A1 (fr) 2002-07-18

Family

ID=9906486

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/000063 Ceased WO2002055539A1 (fr) 2001-01-09 2002-01-07 Procede de concentration de proteines insolubles par filtrage a membrane vibrante

Country Status (9)

Country Link
US (1) US6939697B2 (fr)
EP (1) EP1349868B1 (fr)
JP (1) JP4074519B2 (fr)
AT (1) ATE278706T1 (fr)
CA (1) CA2433689A1 (fr)
DE (1) DE60201496T2 (fr)
ES (1) ES2229091T3 (fr)
GB (1) GB0100513D0 (fr)
WO (1) WO2002055539A1 (fr)

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013158378A3 (fr) * 2012-04-19 2014-06-19 Lockheed Martin Corporation Membranes en graphène perforées de manière sélective pour la collecte, la capture et la rétention d'un constituant
US9028663B2 (en) 2012-03-21 2015-05-12 Lockheed Martin Corporation Molecular separation device
US9610546B2 (en) 2014-03-12 2017-04-04 Lockheed Martin Corporation Separation membranes formed from perforated graphene and methods for use thereof
US9744617B2 (en) 2014-01-31 2017-08-29 Lockheed Martin Corporation Methods for perforating multi-layer graphene through ion bombardment
US9834809B2 (en) 2014-02-28 2017-12-05 Lockheed Martin Corporation Syringe for obtaining nano-sized materials for selective assays and related methods of use
US9833748B2 (en) 2010-08-25 2017-12-05 Lockheed Martin Corporation Perforated graphene deionization or desalination
US9844757B2 (en) 2014-03-12 2017-12-19 Lockheed Martin Corporation Separation membranes formed from perforated graphene and methods for use thereof
US9870895B2 (en) 2014-01-31 2018-01-16 Lockheed Martin Corporation Methods for perforating two-dimensional materials using a broad ion field
US10005038B2 (en) 2014-09-02 2018-06-26 Lockheed Martin Corporation Hemodialysis and hemofiltration membranes based upon a two-dimensional membrane material and methods employing same
US10017852B2 (en) 2016-04-14 2018-07-10 Lockheed Martin Corporation Method for treating graphene sheets for large-scale transfer using free-float method
WO2018185048A1 (fr) * 2017-04-03 2018-10-11 Novozymes A/S Procédé de récupération
EP2401062B1 (fr) * 2009-02-25 2018-10-24 GEA Mechanical Equipment GmbH Procédé et dispositif de filtration
US10118130B2 (en) 2016-04-14 2018-11-06 Lockheed Martin Corporation Two-dimensional membrane structures having flow passages
US10203295B2 (en) 2016-04-14 2019-02-12 Lockheed Martin Corporation Methods for in situ monitoring and control of defect formation or healing
US10201784B2 (en) 2013-03-12 2019-02-12 Lockheed Martin Corporation Method for forming perforated graphene with uniform aperture size
US10213746B2 (en) 2016-04-14 2019-02-26 Lockheed Martin Corporation Selective interfacial mitigation of graphene defects
US10376845B2 (en) 2016-04-14 2019-08-13 Lockheed Martin Corporation Membranes with tunable selectivity
US10418143B2 (en) 2015-08-05 2019-09-17 Lockheed Martin Corporation Perforatable sheets of graphene-based material
US10471199B2 (en) 2013-06-21 2019-11-12 Lockheed Martin Corporation Graphene-based filter for isolating a substance from blood
US10500546B2 (en) 2014-01-31 2019-12-10 Lockheed Martin Corporation Processes for forming composite structures with a two-dimensional material using a porous, non-sacrificial supporting layer
US10653824B2 (en) 2012-05-25 2020-05-19 Lockheed Martin Corporation Two-dimensional materials and uses thereof
US10696554B2 (en) 2015-08-06 2020-06-30 Lockheed Martin Corporation Nanoparticle modification and perforation of graphene
US10980919B2 (en) 2016-04-14 2021-04-20 Lockheed Martin Corporation Methods for in vivo and in vitro use of graphene and other two-dimensional materials

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010502425A (ja) * 2006-08-30 2010-01-28 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 膜を評価するシステム及び方法並びに膜濾過装置
DE602008004747D1 (de) * 2007-02-22 2011-03-10 Baxter Int Verfahren zur aufreinigung hydrophober proteine
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8158405B2 (en) * 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
EP3150628A1 (fr) 2010-04-30 2017-04-05 Asahi Kasei Pharma Corporation Thrombomoduline soluble hautement purifiée

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4872988A (en) * 1988-02-02 1989-10-10 Culkin Joseph B Method and device for separation of colloidal suspensions
EP0388546A1 (fr) * 1989-03-10 1990-09-26 Joseph Bradley Culkin Appareil et méthode pour filtrer des suspensions colloîdales
US5468844A (en) * 1994-01-07 1995-11-21 Protose Technologies Inc. Process for the membrane-filtering of protein solutions
WO1998009717A1 (fr) * 1996-09-06 1998-03-12 Pall Corporation Procede et systeme de separation par cisaillement

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900449A (en) * 1987-05-20 1990-02-13 Gelman Sciences Filtration membranes and method of making the same
US5911880A (en) * 1995-12-15 1999-06-15 Research Corporation Technologies, Inc. Self-wetting membranes from engineering plastics

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4872988A (en) * 1988-02-02 1989-10-10 Culkin Joseph B Method and device for separation of colloidal suspensions
EP0388546A1 (fr) * 1989-03-10 1990-09-26 Joseph Bradley Culkin Appareil et méthode pour filtrer des suspensions colloîdales
US5468844A (en) * 1994-01-07 1995-11-21 Protose Technologies Inc. Process for the membrane-filtering of protein solutions
WO1998009717A1 (fr) * 1996-09-06 1998-03-12 Pall Corporation Procede et systeme de separation par cisaillement

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; HURWITZ, MARK F. ET AL: "Shear separation: a promising method for protein fractionation", XP002200652, retrieved from STN Database accession no. 133:29905 CA *
LAIT (2000), 80(1), 121-127, 2000 *

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2401062B1 (fr) * 2009-02-25 2018-10-24 GEA Mechanical Equipment GmbH Procédé et dispositif de filtration
US9833748B2 (en) 2010-08-25 2017-12-05 Lockheed Martin Corporation Perforated graphene deionization or desalination
US9028663B2 (en) 2012-03-21 2015-05-12 Lockheed Martin Corporation Molecular separation device
WO2013158378A3 (fr) * 2012-04-19 2014-06-19 Lockheed Martin Corporation Membranes en graphène perforées de manière sélective pour la collecte, la capture et la rétention d'un constituant
US10653824B2 (en) 2012-05-25 2020-05-19 Lockheed Martin Corporation Two-dimensional materials and uses thereof
US10201784B2 (en) 2013-03-12 2019-02-12 Lockheed Martin Corporation Method for forming perforated graphene with uniform aperture size
US10471199B2 (en) 2013-06-21 2019-11-12 Lockheed Martin Corporation Graphene-based filter for isolating a substance from blood
US9744617B2 (en) 2014-01-31 2017-08-29 Lockheed Martin Corporation Methods for perforating multi-layer graphene through ion bombardment
US9870895B2 (en) 2014-01-31 2018-01-16 Lockheed Martin Corporation Methods for perforating two-dimensional materials using a broad ion field
US10500546B2 (en) 2014-01-31 2019-12-10 Lockheed Martin Corporation Processes for forming composite structures with a two-dimensional material using a porous, non-sacrificial supporting layer
US9834809B2 (en) 2014-02-28 2017-12-05 Lockheed Martin Corporation Syringe for obtaining nano-sized materials for selective assays and related methods of use
US9844757B2 (en) 2014-03-12 2017-12-19 Lockheed Martin Corporation Separation membranes formed from perforated graphene and methods for use thereof
US9610546B2 (en) 2014-03-12 2017-04-04 Lockheed Martin Corporation Separation membranes formed from perforated graphene and methods for use thereof
US10005038B2 (en) 2014-09-02 2018-06-26 Lockheed Martin Corporation Hemodialysis and hemofiltration membranes based upon a two-dimensional membrane material and methods employing same
US10418143B2 (en) 2015-08-05 2019-09-17 Lockheed Martin Corporation Perforatable sheets of graphene-based material
US10696554B2 (en) 2015-08-06 2020-06-30 Lockheed Martin Corporation Nanoparticle modification and perforation of graphene
US10118130B2 (en) 2016-04-14 2018-11-06 Lockheed Martin Corporation Two-dimensional membrane structures having flow passages
US10203295B2 (en) 2016-04-14 2019-02-12 Lockheed Martin Corporation Methods for in situ monitoring and control of defect formation or healing
US10213746B2 (en) 2016-04-14 2019-02-26 Lockheed Martin Corporation Selective interfacial mitigation of graphene defects
US10376845B2 (en) 2016-04-14 2019-08-13 Lockheed Martin Corporation Membranes with tunable selectivity
US10017852B2 (en) 2016-04-14 2018-07-10 Lockheed Martin Corporation Method for treating graphene sheets for large-scale transfer using free-float method
US10981120B2 (en) 2016-04-14 2021-04-20 Lockheed Martin Corporation Selective interfacial mitigation of graphene defects
US10980919B2 (en) 2016-04-14 2021-04-20 Lockheed Martin Corporation Methods for in vivo and in vitro use of graphene and other two-dimensional materials
WO2018185048A1 (fr) * 2017-04-03 2018-10-11 Novozymes A/S Procédé de récupération

Also Published As

Publication number Publication date
JP4074519B2 (ja) 2008-04-09
CA2433689A1 (fr) 2002-07-18
JP2004532183A (ja) 2004-10-21
ES2229091T3 (es) 2005-04-16
EP1349868B1 (fr) 2004-10-06
EP1349868A1 (fr) 2003-10-08
DE60201496T2 (de) 2006-03-09
ATE278706T1 (de) 2004-10-15
US20040072314A1 (en) 2004-04-15
DE60201496D1 (de) 2004-11-11
GB0100513D0 (en) 2001-02-21
US6939697B2 (en) 2005-09-06

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