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WO2002048187A3 - Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins - Google Patents

Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins

Info

Publication number
WO2002048187A3
WO2002048187A3 PCT/EP2001/014588 EP0114588W WO0248187A3 WO 2002048187 A3 WO2002048187 A3 WO 2002048187A3 EP 0114588 W EP0114588 W EP 0114588W WO 0248187 A3 WO0248187 A3 WO 0248187A3
Authority
WO
WIPO (PCT)
Prior art keywords
eukaryotic cells
proteins
same
expression vectors
secretion signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/014588
Other languages
German (de)
French (fr)
Other versions
WO2002048187A2 (en
Inventor
Manfred Schmitt
Uwe Woelk
Peter Wagner
Tatjana Zagorc
Tanja Heintel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phylos Inc
Original Assignee
Phylos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phylos Inc filed Critical Phylos Inc
Priority to AU2002235771A priority Critical patent/AU2002235771A1/en
Publication of WO2002048187A2 publication Critical patent/WO2002048187A2/en
Publication of WO2002048187A3 publication Critical patent/WO2002048187A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to peptidic secretion signal sequences and nucleic acids coding for the same, to expression vectors for eukaryotic cells, to cell constructs which are transfected with these vectors and to the use of said vectors and cell constructs for the efficient biotechnological production of proteins with eukaryotic cells.
PCT/EP2001/014588 2000-12-14 2001-12-12 Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins Ceased WO2002048187A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002235771A AU2002235771A1 (en) 2000-12-14 2001-12-12 Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000162302 DE10062302A1 (en) 2000-12-14 2000-12-14 Secretion signal peptides, their DNA sequences, expression vectors which can be produced therewith for eukaryotic cells and their use for the biotechnological production of proteins
DE10062302.6 2000-12-14

Publications (2)

Publication Number Publication Date
WO2002048187A2 WO2002048187A2 (en) 2002-06-20
WO2002048187A3 true WO2002048187A3 (en) 2003-01-30

Family

ID=7667112

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/014588 Ceased WO2002048187A2 (en) 2000-12-14 2001-12-12 Secretion signal peptides, their dna sequences, expression vectors for eukaryotic cells that can be produced with the same, and use thereof for biotechnological production of proteins

Country Status (3)

Country Link
AU (1) AU2002235771A1 (en)
DE (1) DE10062302A1 (en)
WO (1) WO2002048187A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10252245A1 (en) * 2002-11-07 2004-05-27 Prof. Dr. Danilo Porro Università degli Studi di Milano-Bicocca Dipartimento die Biotechnologie e Bioscienze Producing a protein useful as pharmaceuticals, e.g. medicine and vaccine, or in food or paper production, comprises expressing and secreting a protein expressed by Zygosaccharomyces bailii strain
WO2010015722A1 (en) 2008-08-08 2010-02-11 Vib Vzw Cells producing glycoproteins having altered glycosylation patterns and methods and use thereof
EP2617732A1 (en) 2012-01-19 2013-07-24 Vib Vzw Tools and methods for expression of membrane proteins
JP6553042B2 (en) 2013-09-05 2019-07-31 ブイアイビー ブイゼットダブリュＶｉｂ Ｖｚｗ Cells producing Fc-containing molecules with altered glycosylation patterns and methods and uses thereof
CN108026558B (en) 2015-07-09 2021-08-17 非营利性组织佛兰芒综合大学生物技术研究所 Cells producing glycoproteins with altered N-and O-glycosylation patterns, and methods and uses thereof
GB201901608D0 (en) 2019-02-06 2019-03-27 Vib Vzw Vaccine adjuvant conjugates

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054339A1 (en) * 1997-05-27 1998-12-03 Hanil Synthetic Fiber Co., Ltd. PROCESS FOR PREPARING RECOMBINANT PROTEINS USING HIGHLY EFFICIENT EXPRESSION VECTOR FROM $i(SACCHAROMYCES CEREVISIAE)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998054339A1 (en) * 1997-05-27 1998-12-03 Hanil Synthetic Fiber Co., Ltd. PROCESS FOR PREPARING RECOMBINANT PROTEINS USING HIGHLY EFFICIENT EXPRESSION VECTOR FROM $i(SACCHAROMYCES CEREVISIAE)

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BASI G ET AL: "TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.", GENE. NETHERLANDS 15 JAN 1993, vol. 123, no. 1, 15 January 1993 (1993-01-15), pages 131 - 136, XP002211006, ISSN: 0378-1119 *
CARTWRIGHT C P ET AL: "Use of beta-lactamase as a secreted reporter of promoter function in yeast.", YEAST (CHICHESTER, ENGLAND) ENGLAND APR 1994, vol. 10, no. 4, April 1994 (1994-04-01), pages 497 - 508, XP001089672, ISSN: 0749-503X *
HEINTEL T ET AL: "Expression, processing and high level secretion of a virus toxin in fission yeast.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. GERMANY JUL 2001, vol. 56, no. 1-2, July 2001 (2001-07-01), pages 165 - 172, XP002211010, ISSN: 0175-7598 *
LEE JEEWON ET AL: "Novel secretion system of recombinant Saccharomyces cerevisiae using an N-terminus residue of human IL-1beta as secretion enhancer.", BIOTECHNOLOGY PROGRESS, vol. 15, no. 5, 1999, pages 884 - 890, XP002211009, ISSN: 8756-7938 *
MAUNDRELL K: "nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 5 JUL 1990, vol. 265, no. 19, 5 July 1990 (1990-07-05), pages 10857 - 10864, XP002211007, ISSN: 0021-9258 *
MAUNDRELL K: "Thiamine-repressible expression vectors pREP and pRIP for fission yeast.", GENE. NETHERLANDS 15 JAN 1993, vol. 123, no. 1, 15 January 1993 (1993-01-15), pages 127 - 130, XP001074164, ISSN: 0378-1119 *
SCHMITT M J ET AL: "Sequence of the M28 dsRNA: preprotoxin is processed to an alpha/beta heterodimeric protein toxin.", VIROLOGY. UNITED STATES 10 NOV 1995, vol. 213, no. 2, 10 November 1995 (1995-11-10), pages 341 - 351, XP002211003, ISSN: 0042-6822 *
SCHMITT MANFRED J: "Cloning and expression of a cDNA copy of the viral K-28 killer toxin gene in yeast.", MOLECULAR & GENERAL GENETICS, vol. 246, no. 2, 1995, pages 236 - 246, XP001074299, ISSN: 0026-8925 *
SCMITT, J. M. & EISFELD, K.: "'Killer' viruses in Saccharamyces cerevisiae and their general importance in understanding eukaryotic cell biology.", RECENT RES. VIROL., vol. 1, 1999, pages 525 - 545, XP008007237 *
TOKUNAGA M ET AL: "STRUCTURE OF YEAST PGKL 128-KDA KILLER-TOXIN SECRETION SIGNAL SEQUENCE PROCESSING OF THE 128-KDA KILLER-TOXIN-SECRETION-SIGNAL-ALPHA-AMYLASE FUSION PROTEIN", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 203, no. 3, 1992, pages 415 - 424, XP008007305, ISSN: 0014-2956 *

Also Published As

Publication number Publication date
AU2002235771A1 (en) 2002-06-24
WO2002048187A2 (en) 2002-06-20
DE10062302A1 (en) 2002-07-11

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