[go: up one dir, main page]

WO2002046444A3 - Double transposition methods for manipulating nucleic acids - Google Patents

Double transposition methods for manipulating nucleic acids Download PDF

Info

Publication number
WO2002046444A3
WO2002046444A3 PCT/US2001/046311 US0146311W WO0246444A3 WO 2002046444 A3 WO2002046444 A3 WO 2002046444A3 US 0146311 W US0146311 W US 0146311W WO 0246444 A3 WO0246444 A3 WO 0246444A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
manipulating nucleic
transposase
methods
transposition methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/046311
Other languages
French (fr)
Other versions
WO2002046444A2 (en
Inventor
Igor Yu Goryshin
Todd A Naumann
William S Reznikoff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Priority to AU2002225888A priority Critical patent/AU2002225888A1/en
Priority to EP01995337A priority patent/EP1339863A2/en
Priority to CA002431246A priority patent/CA2431246A1/en
Publication of WO2002046444A2 publication Critical patent/WO2002046444A2/en
Publication of WO2002046444A3 publication Critical patent/WO2002046444A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.
PCT/US2001/046311 2000-12-05 2001-12-05 Double transposition methods for manipulating nucleic acids Ceased WO2002046444A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002225888A AU2002225888A1 (en) 2000-12-05 2001-12-05 Double transposition methods for manipulating nucleic acids
EP01995337A EP1339863A2 (en) 2000-12-05 2001-12-05 Double transposition methods for manipulating nucleic acids
CA002431246A CA2431246A1 (en) 2000-12-05 2001-12-05 Double transposition methods for manipulating nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25148200P 2000-12-05 2000-12-05
US60/251,482 2000-12-05

Publications (2)

Publication Number Publication Date
WO2002046444A2 WO2002046444A2 (en) 2002-06-13
WO2002046444A3 true WO2002046444A3 (en) 2003-01-09

Family

ID=22952149

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046311 Ceased WO2002046444A2 (en) 2000-12-05 2001-12-05 Double transposition methods for manipulating nucleic acids

Country Status (5)

Country Link
US (2) US7067644B2 (en)
EP (1) EP1339863A2 (en)
AU (1) AU2002225888A1 (en)
CA (1) CA2431246A1 (en)
WO (1) WO2002046444A2 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2355459B (en) 1999-11-29 2001-09-26 Isis Innovation A dominant conditional lethal genetic system
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
GB2402675B (en) * 2003-05-12 2008-02-20 Oxitec Ltd Resistance dilution
GB2441249B (en) * 2003-07-01 2008-04-09 Oxitec Ltd Stable integrands
GB2403475B (en) * 2003-07-01 2008-02-06 Oxitec Ltd Stable integrands
GB2404382B (en) 2003-07-28 2008-01-30 Oxitec Ltd Pest control
WO2005062881A2 (en) * 2003-12-24 2005-07-14 Transgenrx, Inc. Gene therapy using transposon-based vectors
GB0419628D0 (en) * 2004-09-03 2004-10-06 European Molecular Biology Lab Embl Method for determining protein solubility
GB2434344B (en) * 2006-01-20 2009-09-30 Jcb Compact Products Ltd Working machine
GB2443186A (en) 2006-10-25 2008-04-30 Oxitec Ltd Expression system for mediating alternative splicing
PL216037B1 (en) 2006-06-09 2014-02-28 Inst Biotechnologii I Antybiotykow Expression cassette, vector, host cell, the manner of obtaining polypeptide and application
WO2009032664A2 (en) * 2007-08-31 2009-03-12 The Regents Of The University Of Michigan Methods and compositions for increasing biological molecule stability
US9150880B2 (en) 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
US9157097B2 (en) 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2963709B1 (en) * 2008-10-24 2017-05-24 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010118360A1 (en) 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
US20100298170A1 (en) * 2009-05-13 2010-11-25 Nicholas Jack Heredia Methods and systems for introducing functional polynucleotides into a target polynucleotide
GB2500113A (en) 2012-03-05 2013-09-11 Oxitec Ltd Arthropod male germline gene expression system
GB201303932D0 (en) 2013-03-05 2013-04-17 Oxitec Ltd Muscle actin promoter
GB2526867A (en) 2014-06-05 2015-12-09 Oxitec Ltd Gene expression system
DK3334841T3 (en) 2015-08-12 2020-01-20 Cemm Forschungszentrum Fuer Molekulare Medizin Gmbh METHOD OF INVESTIGATING NUCLEIC ACIDS
US11827876B2 (en) 2016-08-12 2023-11-28 Oxitec Ltd. Self-limiting, sex-specific gene and methods of using
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
KR102523318B1 (en) 2016-12-16 2023-04-18 비-모젠 바이오테크놀로지스, 인크. Enhanced HAT family transposon-mediated gene delivery and associated compositions, systems, and methods
EP4060041B1 (en) 2017-09-25 2023-12-27 Fred Hutchinson Cancer Center High efficiency targeted in situ genome-wide profiling
AR117409A1 (en) 2018-03-29 2021-08-04 Oxitec Ltd SELF-LIMITING NIGHTS
CA3104288A1 (en) 2018-06-21 2019-12-26 B-Mogen Biotechnologies, Inc. Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods
AU2020342793A1 (en) 2019-09-06 2022-04-21 Cemm - Forschungszentrum Für Molekulare Medizin Gmbh Method for sequencing RNA oligonucleotides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998010077A1 (en) * 1996-09-09 1998-03-12 Wisconsin Alumni Research Foundation System for in vitro transposition using modified tn5 transposase
WO2000017343A1 (en) * 1998-09-23 2000-03-30 Wisconsin Alumni Research Foundation Method for making insertional mutations

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
CA2380850A1 (en) 1999-08-02 2001-02-08 Wisconsin Alumni Research Foundation Mutant tn5 transposase enzymes and method for their use
US6406896B1 (en) * 1999-08-02 2002-06-18 Wisconsin Alumni Research Foundation Transposase enzyme and method for use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998010077A1 (en) * 1996-09-09 1998-03-12 Wisconsin Alumni Research Foundation System for in vitro transposition using modified tn5 transposase
WO2000017343A1 (en) * 1998-09-23 2000-03-30 Wisconsin Alumni Research Foundation Method for making insertional mutations

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BHASIN ARCHNA ET AL: "Hairpin formation in Tn5 transposition.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 52, 24 December 1999 (1999-12-24), pages 37021 - 37029, XP002213929, ISSN: 0021-9258 *
GORYSHIN I Y ET AL: "Tn5 in vitro transposition", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 13, 27 March 1998 (1998-03-27), pages 7367 - 7374, XP002129603, ISSN: 0021-9258 *
GORYSHIN IGOR Y ET AL: "Tn5/IS50 target recognition.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 95, no. 18, Sept. 1, 1998, pages 10716 - 10721, XP001097740, ISSN: 0027-8424 *
HOFFMAN LES M ET AL: "Transposome insertional mutagenesis and direct sequencing of microbial genomes.", GENETICA (DORDRECHT), vol. 108, no. 1, 2000, pages 19 - 24, XP002213932, ISSN: 0016-6707 *
MAGARSHAK Y: "Double transpositions and the secondary structures of RNA.", BIOPHYSICS (ENGLISH TRANSLATION OF BIOFIZIKA), vol. 38, no. 5, 1993, pages 913 - 914, XP002213930, ISSN: 0006-3509 *
SAKAI J ET AL: "Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transposition", EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 14, no. 17, 1 September 1995 (1995-09-01), pages 4374 - 4383-4384, XP002129605, ISSN: 0261-4189 *

Also Published As

Publication number Publication date
AU2002225888A1 (en) 2002-06-18
WO2002046444A2 (en) 2002-06-13
CA2431246A1 (en) 2002-06-13
US20060223186A1 (en) 2006-10-05
US7338801B2 (en) 2008-03-04
US20020132349A1 (en) 2002-09-19
EP1339863A2 (en) 2003-09-03
US7067644B2 (en) 2006-06-27

Similar Documents

Publication Publication Date Title
WO2002046444A3 (en) Double transposition methods for manipulating nucleic acids
WO2001020035A3 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2205382A1 (en) Enzymatic dna molecules
EP2275546A3 (en) Enzymatic DNA molecules
AU1491401A (en) Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus
DK1419255T3 (en) A new group of alpha-amylases as well as a method for identifying and recovering new alpha-amylases.
PL335860A1 (en) Method of in vitro constructing a dna library
DK1314783T3 (en) Nucleic acid linkers and their use in gene synthesis
WO2005049842A3 (en) Improved targeted dna insertion in plants
WO2002026789A3 (en) Nucleotide sequences mediating male fertility and method of using same
WO2003093306A3 (en) Nucleic acids and proteins from streptococcus groups a & b
US20220170072A1 (en) Programmable dna-guided artificial restriction enzymes
EP1055736A4 (en) DNA SEQUEN AGE PROCESS
WO2002004488A3 (en) Nucleic acid binding polypeptides
WO2002063021A3 (en) Nucleotide sequence mediating male fertility and method of using same
Yamada et al. Nucleotide sequence of initiator tRNA from Bacillus subtilis
CA2356594A1 (en) Method of breeding yeast
WO2001000846A3 (en) Linoleate isomerase
WO2002059353A3 (en) Two-step amplification using a program primer followed by specific primers
WO2001050847A3 (en) Reca / rad51 recombinase-mediated production of recombinant organisms
WO2000056929A3 (en) Coupled polymerase chain reaction-restriction endonuclease digestion-ligase detection reaction process
WO2004016811A3 (en) Methods and kit for genes shuffling using tagged primers
WO2002024904A3 (en) Method for producing ribonucleic acid (rna)
DE69926648D1 (en) Nucleic Acid Isolation
WO2002059366A3 (en) Rna polymers and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2431246

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001995337

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001995337

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2001995337

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP