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WO2002046360A3 - Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent - Google Patents

Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent Download PDF

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Publication number
WO2002046360A3
WO2002046360A3 PCT/UA2001/000041 UA0100041W WO0246360A3 WO 2002046360 A3 WO2002046360 A3 WO 2002046360A3 UA 0100041 W UA0100041 W UA 0100041W WO 0246360 A3 WO0246360 A3 WO 0246360A3
Authority
WO
WIPO (PCT)
Prior art keywords
causative agent
producing
culture medium
mass
tuberculosis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/UA2001/000041
Other languages
French (fr)
Russian (ru)
Other versions
WO2002046360A2 (en
Inventor
Vladimir Vasiljevich Vlasenko
Petr Ivanovich Bagriy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU2002225587A priority Critical patent/AU2002225587A1/en
Priority to EA200300549A priority patent/EA005527B1/en
Publication of WO2002046360A2 publication Critical patent/WO2002046360A2/en
Publication of WO2002046360A3 publication Critical patent/WO2002046360A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention can be used for scientific and production medical and veterinary laboratories. The inventive growth stimulator for a tuberculosis causative agent comprises chemical compositions containing the following elements: sodium, oxygen, hydrogen, and carbon. Said chemical composition also contains the following components (in mass %): chemically pure saccharose 2.0-5.0, rectified alcohol 0.1-0.9, sodium bicarbonate 0.05-0.15, twice-substituted sodium pyrocitrate 0.005-0.9 and a solvent (up to 1000 ml). The growth stimulator can also contain calcium chloride 0.1-0.9 mass %, potato starch 0.01-0.4 mass % and dehydrated glucose 0.01-0.02 mass %. The inventive culture medium for the tuberculosis causative agent in the form of an aqueous solution contains (in mass %) the following components: dry enzymatic peptone, agar-agar or microbiological agar 1.4-1.6, potato starch 1.377- 1.577, calcium dependent enzymes stimulator 0.012-0.014, sodium carbonate 0.005-0.015, the growth stimulator for the tuberculosis causative agent 10-18 and up to 100 of solvent. The method for producing a culture medium and the method for producing the tuberculosis causative agent on then culture medium are also disclosed. The invention makes it possible to reduce time for biological studies preserving a high test's sensivity.
PCT/UA2001/000041 2000-11-03 2001-11-02 Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent Ceased WO2002046360A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002225587A AU2002225587A1 (en) 2000-11-03 2001-11-02 Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent
EA200300549A EA005527B1 (en) 2000-11-03 2001-11-02 Growth stimulator for a tuberculosis causative agent, a culture medium for producing the tuberculosis causative agent, method for producing said medium, method for producing tuberculosis causative agent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
UA2000116242 2000-11-03
UA2000116242A UA43467C2 (en) 2000-11-03 2000-11-03 Stimulators of Mycobacterium tuberculosis "liquid" (option), a breeding ground for the allocation of Mycobacterium tuberculosis (Option) METHOD FOR PRODUCING nutrient medium (option) way to select Mycobacterium tuberculosis in a nutrient medium (option)

Publications (2)

Publication Number Publication Date
WO2002046360A2 WO2002046360A2 (en) 2002-06-13
WO2002046360A3 true WO2002046360A3 (en) 2002-09-26

Family

ID=34391045

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/UA2001/000041 Ceased WO2002046360A2 (en) 2000-11-03 2001-11-02 Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent

Country Status (4)

Country Link
AU (1) AU2002225587A1 (en)
EA (1) EA005527B1 (en)
UA (1) UA43467C2 (en)
WO (1) WO2002046360A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2989003A (en) * 1958-09-25 1961-06-20 Cav Ltd Liquid fuel pumps for internal combustion engines
JPS5558092A (en) * 1978-10-27 1980-04-30 Mitsui Toatsu Chem Inc Method of culturing tubercle bacillus
CN1103893A (en) * 1993-12-14 1995-06-21 中国科学院上海植物生理研究所 Dry substratum for Mycobacterium and the prodn. method
RU2099417C1 (en) * 1993-01-06 1997-12-20 Центральный научно-исследовательский институт туберкулеза РАМН Pyrazineamide-containing nutrient medium for determination of medicinal resistance of tuberculosis pathogen and their identification
RU2106879C1 (en) * 1995-10-03 1998-03-20 Научно-исследовательский институт по изучению лепры Минздравмедпрома РФ Method for culturing slowly growing mycobacteria
RU2117046C1 (en) * 1993-03-09 1998-08-10 Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии Finn-ii-base medium nutrient medium for the accelerated isolation of tuberculosis pathogen from extrapulmonary localization foci

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2989003A (en) * 1958-09-25 1961-06-20 Cav Ltd Liquid fuel pumps for internal combustion engines
JPS5558092A (en) * 1978-10-27 1980-04-30 Mitsui Toatsu Chem Inc Method of culturing tubercle bacillus
RU2099417C1 (en) * 1993-01-06 1997-12-20 Центральный научно-исследовательский институт туберкулеза РАМН Pyrazineamide-containing nutrient medium for determination of medicinal resistance of tuberculosis pathogen and their identification
RU2117046C1 (en) * 1993-03-09 1998-08-10 Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии Finn-ii-base medium nutrient medium for the accelerated isolation of tuberculosis pathogen from extrapulmonary localization foci
CN1103893A (en) * 1993-12-14 1995-06-21 中国科学院上海植物生理研究所 Dry substratum for Mycobacterium and the prodn. method
RU2106879C1 (en) * 1995-10-03 1998-03-20 Научно-исследовательский институт по изучению лепры Минздравмедпрома РФ Method for culturing slowly growing mycobacteria

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BIRGERA M.O.: "Spravochnik po mikrobiologicheskim i virusologicheskim metodam issledovaliya", MEDITSINA, 1982, MOSCOW, pages 79 - 80 *

Also Published As

Publication number Publication date
EA005527B1 (en) 2005-04-28
UA43467C2 (en) 2001-12-17
AU2002225587A1 (en) 2002-06-18
EA200300549A1 (en) 2003-10-30
WO2002046360A2 (en) 2002-06-13

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