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WO2002040126A3 - Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization - Google Patents

Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization Download PDF

Info

Publication number
WO2002040126A3
WO2002040126A3 PCT/US2001/030743 US0130743W WO0240126A3 WO 2002040126 A3 WO2002040126 A3 WO 2002040126A3 US 0130743 W US0130743 W US 0130743W WO 0240126 A3 WO0240126 A3 WO 0240126A3
Authority
WO
WIPO (PCT)
Prior art keywords
target nucleic
fluorescence polarization
methods
nucleic acids
defined positions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/030743
Other languages
French (fr)
Other versions
WO2002040126A2 (en
Inventor
Ness Jeffrey Van
David J Galas
Lori K Garrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Keck Graduate Institute of Applied Life Sciences
Original Assignee
Keck Graduate Institute of Applied Life Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU3922802A priority Critical patent/AU3922802A/en
Application filed by Keck Graduate Institute of Applied Life Sciences filed Critical Keck Graduate Institute of Applied Life Sciences
Priority to US10/398,101 priority patent/US20040038256A1/en
Priority to AU2002239228A priority patent/AU2002239228A1/en
Publication of WO2002040126A2 publication Critical patent/WO2002040126A2/en
Anticipated expiration legal-status Critical
Publication of WO2002040126A3 publication Critical patent/WO2002040126A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention provides a method for identifying a nucleotide at a defined position in a target nucleic acid using restriction endonucleases and fluorescence polarization. The invention further provides compounds, compositions, and kits related to the method.
PCT/US2001/030743 2000-10-02 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization Ceased WO2002040126A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU3922802A AU3922802A (en) 2000-10-02 2001-08-31 Methods for identifying nucleotides at defined positions in target nucleic acidsusing fluorescence polarization
US10/398,101 US20040038256A1 (en) 2001-10-01 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization
AU2002239228A AU2002239228A1 (en) 2000-10-02 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization

Applications Claiming Priority (22)

Application Number Priority Date Filing Date Title
US23740900P 2000-10-02 2000-10-02
US60/237,409 2000-10-02
US24727500P 2000-11-10 2000-11-10
US24717200P 2000-11-10 2000-11-10
US24717300P 2000-11-10 2000-11-10
US24716700P 2000-11-10 2000-11-10
US24716600P 2000-11-10 2000-11-10
US60/247,173 2000-11-10
US60/247,166 2000-11-10
US60/247,167 2000-11-10
US60/247,172 2000-11-10
US60/247,275 2000-11-10
US26397101P 2001-01-24 2001-01-24
US60/263,971 2001-01-24
US26924401P 2001-02-15 2001-02-15
US60/269,244 2001-02-15
US30031901P 2001-06-21 2001-06-21
US30035001P 2001-06-21 2001-06-21
US60/300,319 2001-06-21
US60/300,350 2001-06-21
US30139401P 2001-06-27 2001-06-27
US60/301,394 2001-06-27

Publications (2)

Publication Number Publication Date
WO2002040126A2 WO2002040126A2 (en) 2002-05-23
WO2002040126A3 true WO2002040126A3 (en) 2006-04-06

Family

ID=27582742

Family Applications (4)

Application Number Title Priority Date Filing Date
PCT/US2001/030828 Ceased WO2002028501A1 (en) 2000-10-02 2001-10-01 Genotyping by liquid chromatographic analysis of short nucleic acid fragments
PCT/US2001/030743 Ceased WO2002040126A2 (en) 2000-10-02 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization
PCT/US2001/042432 Ceased WO2002029006A2 (en) 2000-10-02 2001-10-01 Methods for parallel measurement of genetic variations
PCT/US2001/030742 Ceased WO2002046447A2 (en) 2000-10-02 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2001/030828 Ceased WO2002028501A1 (en) 2000-10-02 2001-10-01 Genotyping by liquid chromatographic analysis of short nucleic acid fragments

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/US2001/042432 Ceased WO2002029006A2 (en) 2000-10-02 2001-10-01 Methods for parallel measurement of genetic variations
PCT/US2001/030742 Ceased WO2002046447A2 (en) 2000-10-02 2001-10-01 Methods for identifying nucleotides at defined positions in target nucleic acids

Country Status (2)

Country Link
AU (4) AU3922802A (en)
WO (4) WO2002028501A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004535815A (en) * 2001-07-15 2004-12-02 ケック グラデュエイト インスティテュート Amplification of nucleic acid fragments using nicking agents
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US7052839B2 (en) 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7041812B2 (en) 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7560254B2 (en) 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
GB0228614D0 (en) * 2002-12-07 2003-01-15 Fu Guoliang Oligonucleotide guided analysis of gene expression
CA2524255C (en) 2003-03-21 2014-02-11 Academisch Ziekenhuis Leiden Modulation of exon recognition in pre-mrna by interfering with the secondary rna structure
PT2203173E (en) 2007-10-26 2016-03-15 Academisch Ziekenhuis Leiden Means and methods for counteracting muscle disorders
USRE48468E1 (en) 2007-10-26 2021-03-16 Biomarin Technologies B.V. Means and methods for counteracting muscle disorders
EP2119783A1 (en) 2008-05-14 2009-11-18 Prosensa Technologies B.V. Method for efficient exon (44) skipping in Duchenne Muscular Dystrophy and associated means
NZ595955A (en) 2009-04-24 2012-10-26 Prosensa Technologies Bv Oligonucleotide comprising an inosine for treating dmd
NZ627896A (en) 2012-01-27 2016-11-25 Biomarin Technologies B V Rna modulating oligonucleotides with improved characteristics for the treatment of duchenne and becker muscular dystrophy

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011220A1 (en) * 1998-08-21 2000-03-02 Washington University Fluorescence polarization in nucleic acid analysis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040166A (en) * 1985-03-28 2000-03-21 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences, including a probe
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1993024613A1 (en) * 1992-05-22 1993-12-09 The United States Government As Represented By The Secretary Of The Department Of Health And Human Services Improved expression of human multidrug resistance genes and improved selection of cells transduced with such genes
US5284758A (en) * 1992-08-14 1994-02-08 Merck & Co., Inc. Process for forming cholesterol lowering compound using pseudodiplodia sp.
JPH078289A (en) * 1993-06-29 1995-01-13 Osaka Gas Co Ltd Method for synthesizing peptide and protein using ribosome
US6110709A (en) * 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
US6027898A (en) * 1997-08-18 2000-02-22 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011220A1 (en) * 1998-08-21 2000-03-02 Washington University Fluorescence polarization in nucleic acid analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEN ET AL.: "Fluorescence polarization in homogeneous nucleic acid analysis", GENOME RESEARCH, vol. 9, 1999, pages 492 - 498, XP000851703 *

Also Published As

Publication number Publication date
WO2002028501A1 (en) 2002-04-11
WO2002029006A2 (en) 2002-04-11
AU2002239228A8 (en) 2006-11-02
WO2002046447A2 (en) 2002-06-13
AU3922802A (en) 2002-05-27
AU1183902A (en) 2002-04-15
AU4146002A (en) 2002-06-18
WO2002029006A3 (en) 2002-08-29
WO2002046447A3 (en) 2003-12-24
WO2002040126A2 (en) 2002-05-23
AU2001296491A1 (en) 2002-04-15

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