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WO2001036935A1 - Dispositif plongeur absorbant destine a l'extraction de solvants - Google Patents

Dispositif plongeur absorbant destine a l'extraction de solvants Download PDF

Info

Publication number
WO2001036935A1
WO2001036935A1 PCT/GB2000/004407 GB0004407W WO0136935A1 WO 2001036935 A1 WO2001036935 A1 WO 2001036935A1 GB 0004407 W GB0004407 W GB 0004407W WO 0136935 A1 WO0136935 A1 WO 0136935A1
Authority
WO
WIPO (PCT)
Prior art keywords
receptacle
absorbent portion
absorbent
pellet
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/004407
Other languages
English (en)
Inventor
John William Gow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Glasgow
Original Assignee
University of Glasgow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Glasgow filed Critical University of Glasgow
Priority to EP00976191A priority Critical patent/EP1236031A1/fr
Priority to AU14068/01A priority patent/AU1406801A/en
Publication of WO2001036935A1 publication Critical patent/WO2001036935A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1037—Using surface tension, e.g. pins or wires

Definitions

  • the present invention relates to a device for, and a method of, removing solution from a receptacle, such as a microcentrifuge tube or micro-titre plate.
  • a receptacle such as a microcentrifuge tube or micro-titre plate.
  • Many life science experiments require the extraction and purification of DNA, RNA or proteins and the methods used in the extractions often include precipitation and centrifugation steps in order to pellet the DNA, RNA or proteins.
  • Conventional means of removing the solution after pelleting involves pouring the solution out of the centrifugation tube.
  • it is an object of the present invention is to provide a device for, and a method of removing solution from a receptacle, which is simple and economical.
  • a device for removing a solution from a receptacle comprising: a first portion comprising holding means; and a second substantially stiff absorbent portion adapted to allow insertion into the receptacle, a first end of which is connected to the first portion, and a second end of which is shaped to substantially prevent contact with a pellet formed in said receptacle when in use.
  • the holding means may be elongate for gripping by a user's fingers.
  • the holding means may be adapted to fit to a further device such as a typical pipettor (eg. Gilson pipette) known in the art.
  • a typical pipettor eg. Gilson pipette
  • the device may have a cylindrical holding means into which the end of a pipettor is inserted.
  • the second end of the absorbent portion is shaped to substantially prevent contact with a pellet which may be formed near the bottom of the receptacle.
  • a pellet which may be formed near the bottom of the receptacle.
  • the second end may be angled and/or channelled to allow the device to absorb any solution present at the bottom of the receptacle while substantially preventing contact with said pellet.
  • the second end may be in the form of a cone or truncated cone. When the receptacle is cylindrical in shape the cone or truncated cone may be similarly circular.
  • the tip of the device may be generally blunt (eg. a frusto- conical) or concave to prevent contact with said pellet.
  • the term "stiff" is intended to mean that the material of the absorbent portion is substantially resistant to compression or bending but is not necessarily rigid.
  • the absorbent portion may be shaped to form a substantially close fit with the internal walls of the receptacle.
  • the absorbent portion may be generally cylindrical or conical- shaped and may be hollow or solid.
  • the absorbent portion is typically elongate in order to reach the bottom of for example microcentrifuge tubes.
  • solution may encompass organic solvents, such as ethanol and the like, inorganic solvents, such as acids, and aqueous solutions, such as water which may or may not contain a salt.
  • the absorbent portion may be designed to absorb organic, inorganic and/or aqueous solvents separately, or may be designed to absorb any/all of the abovementioned solutions. That is, the aforementioned properties need not be mutually exclusive and, in fact, the same absorbent portion may absorb more than one of the above classes of solutions.
  • receptacle may encompass different sized tubes, microcentrifuge tubes, (for example 2ml, 1.5ml or 0.5ml microtubes) , multi-well microtitre plates and the like.
  • the abovementioned pellet may be, for example, a pellet formed from nucleic acids, for example DNA or RNA, or a pellet formed from polypeptides, for example proteins.
  • the absorbent portion may be composed of a material which is neutral or a material which is intended to repel the formed pellet, and therefore substantially prevent removal of the pellet should the absorbent portion contact the pellet.
  • the absorbent portion may be composed of a negatively charged material.
  • the absorbent portion may be composed of a positively charged material.
  • the absorbent portion of the device may be composed of non- ionic or non-polar materials, such as hydrophobic materials.
  • the device may also be colour-coded so that a user may easily identify whether the device is for example positively charged (eg. red) or negatively charged (eg. blue) .
  • the device, or more particularly the absorbent portion may be sterilisable by, for example, heat, gamma irradiation or the like.
  • the portions of the device may be formed of the same material or separate materials.
  • the device may be formed in one integral piece.
  • the portions may be made independent of one another and/or from different materials, which are eventually brought together to form the device.
  • the device may also be "double-ended". That is, with absorbent portions at either end and with one central portion acting as the holding means in use.
  • the device may be formed from, for example, chromatography paper since this possesses good absorbing capabilities.
  • absorbent materials may also be suitable such as hydrogel like materials, cotton, absorbent celluloses such as carboxy methyl cellulose, activated charcoal, nylon, other textiles or composite materials and the like.
  • the absorbent material should not shed any fibres and the like which may contaminate the pellet.
  • the device holding said device by the holding means, inserting the substantially stiff absorbing portion into said receptacle in an orientation so as to substantially avoid contact between said absorbent portion and a pellet formed in said receptacle, leaving said absorbent portion in the said receptacle for a time sufficient to substantially remove said solution, and removing said absorbent portion from said receptacle while substantially avoiding contact between said absorbent portion and said pellet.
  • the device may thereafter be allowed to touch the pellet, when the absorbent material and the pellet are similar charged, as the absorbent material and the pellet will repel each other.
  • Figure 1 is a side perspective elevation of a device in accordance with an embodiment of the present invention
  • Figure 2 is a front view of a device as illustrated in Figure 1 placed in a microcentrifuge tube;
  • Figure 3 is a front view of an alternative embodiment of the present invention placed in a microcentrifuge tube.
  • Figure 4 is a front view of a further embodiment of the present invention placed inside a microcentrifuge tube.
  • the device 10 embodying the invention comprises a generally three dimensional cylindrical-shaped body 12, an
  • SUBSTTTUTE SHEET (RULE 26) asymmetrical conical shaped tip 14 and holding means 16.
  • the device 10 is formed from a flat, two-dimensional template which is subsequently rolled into a tube with flat holding means 16 at the top. The point of the device is constructed to form an asymmetric tip 14.
  • the device 10 is placed within a microcentrifuge tube 18 and the asymmetrical conical shaped tip 14 is orientated in order to avoid contact with pellet 20.
  • Pellets may form on walls of centrifuge tubes depending on the angle of the tube during centrifugation.
  • the pellet 20 of Figure 2 is commonly formed by fixed angle centrifugation where the angle of the tube orientation is approximately 45 degrees from the vertical. Removal of the solution from the microcentrifuge tube 18 occurs when the absorbent portion comes into contact with the solution which is absorbed by the absorbent portion as a result of capillary action. The device 10 is then removed from the microcentrifuge.
  • the device 10 may be manufactured in a single article from materials such as Whatman cellulose/cotton blotting and chromatography paper (3MM) catalogue no. 3030917 (Whatman International, 20/20 Maidstone, Kent, ME16 0LS, United Kingdom) .
  • the device 10 can be inserted with reasonable force without the risk of contacting pellet 20.
  • SUBSTTTUTE SHEET (RULE 26) A second embodiment 10b of the present invention is illustrated in Figure 3.
  • Device 10b has a tip 14b shaped to form a blunt or concave end in order to avoid contact with pellet 20b which has formed at the base of the microcentrifuge tube.
  • Pellets are commonly formed at the base of centrifuge tubes when swing-arm rotors are used. These allow the tube to assume a substantially horizontal position during centrifugation.
  • Device 10c has a symmetrically rounded or convex tip 14c.
  • the sides of absorbent portion of device 10c are not intended to form a close fit with microcentrifuge tube 18. Instead, the device 10c is inserted down the centre of the tube 18 to contact the base of the tube 18 and thereby avoid pellet 20c formed on the wall at the base of tube 18.
  • the device substantially reduces the risk of contaminating the sample with for example fibre deposits which may arise from using paper tissue.
  • the absorbent portion of a device having an asymmetrically formed tip as illustrated in Figure 1
  • the angled tip may be turned around to touch the sample in order to dry the sample as well as the tube. Since the dipper has a similar charge as the sample, they will repel one another and so the sample will not stick to the dipper. This may also apply to the device depicted in Figure 4.
  • the device may be manufactured from two separate materials, each corresponding to the holding means and the absorbing means, which are then brought together to form the complete device.
  • the principal advantage of the present invention is that the device substantially reduces the time, labour and expense required for removal of residual solution in a receptacle.
  • the devices can be sterilised and can therefore reduce or even eliminate contamination of the sample. Moreover, since the device is intended to be used once, this further reduces any possibility of contamination.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif destiné à extraire une solution d'un réceptacle, lequel dispositif comprend un moyen de support et une partie absorbante sensiblement rigide apte à être insérée dans le réceptacle, dont l'extrémité possède une forme telle qu'elle empêche sensiblement le contact avec un sédiment formé dans le réceptacle précité. L'invention se rapporte également à un procédé permettant d'extraire une solution d'un réceptacle à l'aide du dispositif précité.
PCT/GB2000/004407 1999-11-19 2000-11-20 Dispositif plongeur absorbant destine a l'extraction de solvants Ceased WO2001036935A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00976191A EP1236031A1 (fr) 1999-11-19 2000-11-20 Dispositif plongeur absorbant destine a l'extraction de solvants
AU14068/01A AU1406801A (en) 1999-11-19 2000-11-20 Absorbent dipper for solvent extraction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9927267.6 1999-11-19
GBGB9927267.6A GB9927267D0 (en) 1999-11-19 1999-11-19 Absorbent dipper

Publications (1)

Publication Number Publication Date
WO2001036935A1 true WO2001036935A1 (fr) 2001-05-25

Family

ID=10864733

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004407 Ceased WO2001036935A1 (fr) 1999-11-19 2000-11-20 Dispositif plongeur absorbant destine a l'extraction de solvants

Country Status (4)

Country Link
EP (1) EP1236031A1 (fr)
AU (1) AU1406801A (fr)
GB (1) GB9927267D0 (fr)
WO (1) WO2001036935A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008147281A1 (fr) * 2007-05-30 2008-12-04 Ge Healthcare Bio-Sciences Ab Dispositif et procédé de séparation de protéines et d'autres biomolécules

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952518A (en) * 1984-10-01 1990-08-28 Cetus Corporation Automated assay machine and assay tray
EP0394021A2 (fr) * 1989-04-19 1990-10-24 Pall Corporation Dispositif diagnostique avec un seul élément poreux et absorbant
EP0709678A1 (fr) * 1994-10-25 1996-05-01 Sumitomo Pharmaceuticals Company, Limited Plaque de support pour les essais immunologiques
US5760315A (en) * 1994-11-28 1998-06-02 Akzo Nobel N.V. Sample collection device with assay reagent and barrier
WO1999021655A1 (fr) * 1997-10-27 1999-05-06 Idexx Laboratories, Inc. Dispositif et procedes permettant de mesurer un analyte dans une solution

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952518A (en) * 1984-10-01 1990-08-28 Cetus Corporation Automated assay machine and assay tray
EP0394021A2 (fr) * 1989-04-19 1990-10-24 Pall Corporation Dispositif diagnostique avec un seul élément poreux et absorbant
EP0709678A1 (fr) * 1994-10-25 1996-05-01 Sumitomo Pharmaceuticals Company, Limited Plaque de support pour les essais immunologiques
US5760315A (en) * 1994-11-28 1998-06-02 Akzo Nobel N.V. Sample collection device with assay reagent and barrier
WO1999021655A1 (fr) * 1997-10-27 1999-05-06 Idexx Laboratories, Inc. Dispositif et procedes permettant de mesurer un analyte dans une solution

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008147281A1 (fr) * 2007-05-30 2008-12-04 Ge Healthcare Bio-Sciences Ab Dispositif et procédé de séparation de protéines et d'autres biomolécules
US8413528B2 (en) 2007-05-30 2013-04-09 Ge Healthcare Bio-Sciences Ab Device and method for separation of proteins and other biomolecules
US8844385B2 (en) 2007-05-30 2014-09-30 Ge Healthcare Bio-Sciences Ab Device and method for separation of proteins and other biomolecules

Also Published As

Publication number Publication date
EP1236031A1 (fr) 2002-09-04
GB9927267D0 (en) 2000-01-12
AU1406801A (en) 2001-05-30

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