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WO2001036621A3 - Regulation negative et inhibition des genes allergenes dans des graines d'arachide transgeniques - Google Patents

Regulation negative et inhibition des genes allergenes dans des graines d'arachide transgeniques Download PDF

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Publication number
WO2001036621A3
WO2001036621A3 PCT/US2000/031657 US0031657W WO0136621A3 WO 2001036621 A3 WO2001036621 A3 WO 2001036621A3 US 0031657 W US0031657 W US 0031657W WO 0136621 A3 WO0136621 A3 WO 0136621A3
Authority
WO
WIPO (PCT)
Prior art keywords
peanut
allergen
ara
genes
antisense
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/031657
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English (en)
Other versions
WO2001036621A2 (fr
Inventor
Hortense W Dodo
Charles J Arntzen
Koffi N Da Konan
Olga M Viquez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alabama A&M University AAMU
Original Assignee
Alabama A&M University AAMU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alabama A&M University AAMU filed Critical Alabama A&M University AAMU
Priority to EP00982149A priority Critical patent/EP1235920A2/fr
Priority to AU19216/01A priority patent/AU1921601A/en
Publication of WO2001036621A2 publication Critical patent/WO2001036621A2/fr
Publication of WO2001036621A3 publication Critical patent/WO2001036621A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une graine d'arachide transgénique non allergène obtenue par des techniques de recombinaison. Les plantes d'arachide sont transformées à l'aide de multiples copies de chacun des gènes allergènes, ou de leurs fragments, afin de supprimer l'expression génique et la production de protéines allergènes. En variante, les plantes d'arachide sont transformées à l'aide de gènes d'arachide allergènes anti-sens introduits dans le génome de l'arachide comme fragments anti-sens, fragments sens, ou des combinaisons de fragments anti-sens et sens. Les transgènes d'arachide sont régulés par le promoteur 35S ou le promoteur du gène Ara h2 pour produire des ARNs anti-sens, des ARNs sens, et des ARNs double-brin afin de supprimer la production de protéines allergènes dans les plantes d'arachide. Un clone génomique pour l'allergène Ara h2 est isolé et séquencé. L'ORF est de 622 nucléotides. La protéine codée prévue est de 207 acides aminés et comprend un peptide de transit reconnu de 21 résidus. Un signal de polyadénylation est identifié sur la position 951. Six codons d'arrêt supplémentaires sont observés. Une région promoteur s'est avérée contenir une boîte TATA reconnue située sur la position -72. Des régions homologues ont été identifiées entre Ara h2, h6, et h7, et entre Ara h3 et h4, et entre Ara h1P41B et Ara h1P17. Les régions homologues sont utilisées dans le criblage de la bibliothèque génomique de l'arachide afin d'isoler tous les gènes allergènes de l'arachide et pour la régulation négative et l'inhibition des multiples gènes allergènes de l'arachide.
PCT/US2000/031657 1999-11-19 2000-11-20 Regulation negative et inhibition des genes allergenes dans des graines d'arachide transgeniques Ceased WO2001036621A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00982149A EP1235920A2 (fr) 1999-11-19 2000-11-20 Regulation negative et inhibition des genes allergenes dans des graines d'arachide transgeniques
AU19216/01A AU1921601A (en) 1999-11-19 2000-11-20 Down-regulation and silencing of allergen genes in transgenic peanut seeds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16725599P 1999-11-19 1999-11-19
US60/167,255 1999-11-19

Publications (2)

Publication Number Publication Date
WO2001036621A2 WO2001036621A2 (fr) 2001-05-25
WO2001036621A3 true WO2001036621A3 (fr) 2002-01-10

Family

ID=22606602

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/031657 Ceased WO2001036621A2 (fr) 1999-11-19 2000-11-20 Regulation negative et inhibition des genes allergenes dans des graines d'arachide transgeniques

Country Status (3)

Country Link
EP (1) EP1235920A2 (fr)
AU (1) AU1921601A (fr)
WO (1) WO2001036621A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7485708B2 (en) 1996-09-23 2009-02-03 University Of Arkansas Nucleic acids encoding ara h 3 polypeptides
CN102199621B (zh) * 2011-03-11 2013-04-24 山东省花生研究所 根癌农杆菌介导的花生高效转基因方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6943010B1 (en) 1999-11-19 2005-09-13 Hortense W. Dodo Down-regulation and silencing of allergen genes in transgenic peanut seeds
AU2005252268B2 (en) * 2004-06-10 2012-06-28 Aravax Pty Limited Novel immunointeractive molecules and uses thereof
US8057800B2 (en) 2004-06-10 2011-11-15 Circassia Limited Immunointeractive molecules and uses thereof
WO2007123771A2 (fr) * 2006-03-30 2007-11-01 The Regents Of The University Of California Vaccination à adn anti-ige
EP3641892A1 (fr) 2017-06-23 2020-04-29 Mabylon AG Anticorps anti-allergènes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038978A1 (fr) * 1998-01-31 1999-08-05 University Of Arkansas Procedes et reactifs permettant de diminuer les reactions allergiques

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038978A1 (fr) * 1998-01-31 1999-08-05 University Of Arkansas Procedes et reactifs permettant de diminuer les reactions allergiques

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BHALLA PREM L ET AL: "Antisense-mediated silencing of a gene encoding a major ryegrass pollen allergen.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 20, Sept. 28, 1999, pages 11676 - 11680, XP002167601, ISSN: 0027-8424 *
BURKS AW ET AL: "Recombinant peanut allergen Ara h 1 expression and IgE binding in patients with peanut hypersensitivity", JOURNAL OF CLINICAL INVESTIGATION,US,NEW YORK, NY, vol. 96, no. 4, October 1995 (1995-10-01), pages 1715 - 1721, XP002107204, ISSN: 0021-9738 *
DATABASE GENEMBL 27 September 1996 (1996-09-27), KAANG B. K-: "Expression vector pNEX3 beta-lactamase mRa, complete cds.", XP002167602 *
KLEBER-JANKE TAMARA ET AL: "Selective cloning of peanut allergens, including profilin and 2S albumins, by phage display technology.", INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, vol. 119, no. 4, August 1999 (1999-08-01), pages 265 - 274, XP001003024, ISSN: 1018-2438 *
STANLEY J S ET AL: "IDENTIFICATION AND MUTATIONAL ANALYSIS OF THE IMMUNODOMINANT IGE BINDING EPITOPES OF THE MAJOR PEANUT ALLERGEN ARA H 2", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,NEW YORK, US,US, vol. 342, no. 2, 15 June 1997 (1997-06-15), pages 244 - 253, XP000946317, ISSN: 0003-9861 *
TADA YUICHI ET AL: "Reduction of 14-16 kDa allergenic proteins in transgenic rice plants by antisense gene.", FEBS LETTERS, vol. 391, no. 3, 1996, pages 341 - 345, XP000993422, ISSN: 0014-5793 *
VIQUEZ O M ET AL: "Isolation and molecular characterization of the first genomic clone of a major peanut allergen, Ara h 2", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 107, no. 4, April 2001 (2001-04-01), pages 713 - 717, XP001001506 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7485708B2 (en) 1996-09-23 2009-02-03 University Of Arkansas Nucleic acids encoding ara h 3 polypeptides
CN102199621B (zh) * 2011-03-11 2013-04-24 山东省花生研究所 根癌农杆菌介导的花生高效转基因方法

Also Published As

Publication number Publication date
WO2001036621A2 (fr) 2001-05-25
AU1921601A (en) 2001-05-30
EP1235920A2 (fr) 2002-09-04

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