[go: up one dir, main page]

WO2001036659A3 - Genes marqueurs de surface selectionnables - Google Patents

Genes marqueurs de surface selectionnables Download PDF

Info

Publication number
WO2001036659A3
WO2001036659A3 PCT/EP2000/011474 EP0011474W WO0136659A3 WO 2001036659 A3 WO2001036659 A3 WO 2001036659A3 EP 0011474 W EP0011474 W EP 0011474W WO 0136659 A3 WO0136659 A3 WO 0136659A3
Authority
WO
WIPO (PCT)
Prior art keywords
mutated
mammalian cells
ptkr
transduced
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/011474
Other languages
English (en)
Other versions
WO2001036659A2 (fr
Inventor
Susanne Dagmar Pippig
Gabor Veres
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Pharma GmbH Austria
Novartis AG
Original Assignee
Novartis Erfindungen Verwaltungs GmbH
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Erfindungen Verwaltungs GmbH, Novartis AG filed Critical Novartis Erfindungen Verwaltungs GmbH
Priority to CA002391593A priority Critical patent/CA2391593A1/fr
Priority to EP00988723A priority patent/EP1278773A2/fr
Priority to NZ518877A priority patent/NZ518877A/en
Priority to AU25070/01A priority patent/AU779462B2/en
Priority to IL14962100A priority patent/IL149621A0/xx
Priority to JP2001538537A priority patent/JP2003522525A/ja
Publication of WO2001036659A2 publication Critical patent/WO2001036659A2/fr
Anticipated expiration legal-status Critical
Publication of WO2001036659A3 publication Critical patent/WO2001036659A3/fr
Priority to AU2005201764A priority patent/AU2005201764A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un procédé permettant d'identifier des cellules de mammifère modifiées génétiquement à l'aide d'un récepteur de protéine-tyrosine kinase mutante (PTKR) comme marqueur sélectionnable de cellules de mammifère. Les membres de la famille des récepteurs du facteur de croissance épidermique (EGFR), ainsi que les membres de la famille des récepteurs de tyrosine kinase spécifique de muscle (MuSKR) sont des marqueurs sélectifs de PTKR mutant particulièrement préférés. L'invention concerne en outre un procédé d'immunosélection de cellules de mammifère transduites, qui comporte les étapes consistant à : mettre en oeuvre une transduction rétrovirale de cellules de mammifère à l'aide d'une séquence d'acides nucléiques codant pour un EGFR mutant ; incuber les cellules transduites avec un anticorps marqué qui reconnaît le PTKR mutant et se lie spécifiquement à celui-ci ; et identifier les cellules marquées transduites.
PCT/EP2000/011474 1999-11-19 2000-11-17 Genes marqueurs de surface selectionnables Ceased WO2001036659A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002391593A CA2391593A1 (fr) 1999-11-19 2000-11-17 Genes marqueurs de surface selectionnables
EP00988723A EP1278773A2 (fr) 1999-11-19 2000-11-17 Genes marqueurs de surface selectionnables
NZ518877A NZ518877A (en) 1999-11-19 2000-11-17 Selectable cell surface marker genes
AU25070/01A AU779462B2 (en) 1999-11-19 2000-11-17 Selectable cell surface marker genes
IL14962100A IL149621A0 (en) 1999-11-19 2000-11-17 Selectable cell surface marker genes
JP2001538537A JP2003522525A (ja) 1999-11-19 2000-11-17 細胞表面選択マーカー遺伝子
AU2005201764A AU2005201764A1 (en) 1999-11-19 2005-04-27 Selectable cell surface marker genes

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US16659499P 1999-11-19 1999-11-19
US44403899A 1999-11-19 1999-11-19
US09/444,038 1999-11-19
US60/166,594 1999-11-19
US53924800A 2000-03-30 2000-03-30
US09/539,248 2000-03-30

Publications (2)

Publication Number Publication Date
WO2001036659A2 WO2001036659A2 (fr) 2001-05-25
WO2001036659A3 true WO2001036659A3 (fr) 2002-11-14

Family

ID=27389294

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/011474 Ceased WO2001036659A2 (fr) 1999-11-19 2000-11-17 Genes marqueurs de surface selectionnables

Country Status (7)

Country Link
EP (1) EP1278773A2 (fr)
JP (1) JP2003522525A (fr)
AU (1) AU779462B2 (fr)
CA (1) CA2391593A1 (fr)
IL (1) IL149621A0 (fr)
NZ (1) NZ518877A (fr)
WO (1) WO2001036659A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002302740B2 (en) * 2001-05-11 2008-02-21 Medical Research Council Assays for identifying modulators of rhomboid polypeptides
WO2003066677A1 (fr) 2002-02-05 2003-08-14 Japan Science And Technology Agency Complexe egf/egfr
HRP20190556T1 (hr) * 2009-11-03 2019-06-14 City Of Hope SKRAĆENI OBLIK RECEPTORA EPIDERMALNOG FAKTORA RASTA ZA (EGFRt) ODABIR TRANSDUCIRANIH T-STANICA

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006723A1 (fr) * 1993-09-01 1995-03-09 Boehringer Mannheim Gmbh Procede de marquage de cellules eucariotes par utilisation d'un recepteur de surface cellulaire en tant que marqueur
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006723A1 (fr) * 1993-09-01 1995-03-09 Boehringer Mannheim Gmbh Procede de marquage de cellules eucariotes par utilisation d'un recepteur de surface cellulaire en tant que marqueur
US6107477A (en) * 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HILDINGER M ET AL: "Bicistronic retroviral vectors for combining myeloprotection with cell-surface marking.", GENE THERAPY, vol. 6, no. 7, July 1999 (1999-07-01), pages 1222 - 1230, XP000990645, ISSN: 0969-7128 *

Also Published As

Publication number Publication date
AU779462B2 (en) 2005-01-27
JP2003522525A (ja) 2003-07-29
CA2391593A1 (fr) 2001-05-25
NZ518877A (en) 2004-03-26
AU2507001A (en) 2001-05-30
EP1278773A2 (fr) 2003-01-29
IL149621A0 (en) 2002-11-10
WO2001036659A2 (fr) 2001-05-25

Similar Documents

Publication Publication Date Title
Plowman et al. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3
EP1860186A3 (fr) Procédé pour la distinction de cellules souches mésenchymateuses à l'aide de divers marqueurs
DK1244808T3 (da) Fremgangsmåde til screening af multipel transformerede celler ved anvendelse af bicistronisk ekspression af fluorescerende proteiner
JP2001514879A5 (fr)
DK506488D0 (da) Fremgangsmaade til dyrkning af rekombinante celler, vaertscelle til anvendelse ved fremgangsmaaden og kultur omfattende vaertscellen
ATE395424T1 (de) Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
CA2450581A1 (fr) Analyse de l'innocuite cardio-vasculaire
WO2001036659A3 (fr) Genes marqueurs de surface selectionnables
Schuermann An expression vector system for stable expression of oncogenes
Suttle et al. Initiation factor 3 requirement for the formation of initiation complexes with synthetic oligonucleotides
CN105646716A (zh) 一种基因编码的环腺苷酸荧光探针及其制备方法和应用
AU715215B2 (en) Method for the detection of compounds that modulate the effects of the obese protein
Lindsay et al. Enzyme thermostability is a transformable property between Bacillus spp.
CN108796619A (zh) 赖氨酸脱羧酶突变体文库构建及高或低活力突变株高通量筛选的方法
WO2002022874A8 (fr) Formule de sequence oligonucleotidique pour le marquage de sondes oligonucleotidiques et de proteines, destine a une analyse in-situ
Pajunen et al. Assignment of the gene coding for the alpha-subunit of prolyl 4-hydroxylase to human chromosome region 10q21. 3-23.1
Kotelnikova et al. Taxonomic analysis of thermophilic strains of the genus Methanobacterium: reclassification of Methanobacterium thermoalcaliphilum as a synonym of Methanobacterium thermoautotrophicum
Guan et al. Characterization of a complex chromosome rearrangement involving 6q in a melanoma cell line by chromosome microdissection
WO2002018562A1 (fr) Enzymes de type trypsine propres aux voies respiratoires et leur procede d'utilisation
CA2381065A1 (fr) Procede d'enrichissement et/ou d'isolation de neurones dopaminergiques
Leeck et al. The upstream region is required but not sufficient to control mutually exclusive expression of Paramecium surface antigen genes.
DE60132271D1 (de) Verwendung von CD34 oder eines davon abgeleiteten Polypeptids als Oberflächenmarker für Gentransfer
WO2000071711B1 (fr) Genes exprimes de maniere differentielle en cas de cancer de la prostate
HK1039744A1 (zh) 刺激prosaposin受体活性的方法
CN101093226B (zh) 鉴别分泌型蛋白标志物的系统及方法

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2000988723

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 25070/01

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 518877

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 149621

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 2391593

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2001 538537

Country of ref document: JP

Kind code of ref document: A

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWP Wipo information: published in national office

Ref document number: 2000988723

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 518877

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 518877

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 25070/01

Country of ref document: AU

WWW Wipo information: withdrawn in national office

Ref document number: 2000988723

Country of ref document: EP

DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)