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WO2001035747A2 - Utilisation de lignosulphonates pour proteger les plantes - Google Patents

Utilisation de lignosulphonates pour proteger les plantes Download PDF

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Publication number
WO2001035747A2
WO2001035747A2 PCT/EP2000/011529 EP0011529W WO0135747A2 WO 2001035747 A2 WO2001035747 A2 WO 2001035747A2 EP 0011529 W EP0011529 W EP 0011529W WO 0135747 A2 WO0135747 A2 WO 0135747A2
Authority
WO
WIPO (PCT)
Prior art keywords
lignosulphonates
soil
plant
plants
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/011529
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English (en)
Other versions
WO2001035747A3 (fr
Inventor
Wilhelmus Maria Van Der Krieken
Borchert Willem Veen
Cornelis Johannus Kok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plant Research International BV
Original Assignee
Plant Research International BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Research International BV filed Critical Plant Research International BV
Priority to EP00993103A priority Critical patent/EP1229794A2/fr
Priority to AU28366/01A priority patent/AU2836601A/en
Publication of WO2001035747A2 publication Critical patent/WO2001035747A2/fr
Publication of WO2001035747A3 publication Critical patent/WO2001035747A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof

Definitions

  • the present invention relates to plant protection methods, such as weed control, inhibition of water evaporation, control of plant pathogens, such as nematodes, and to compositions and substrates for use in these methods.
  • herbicide application has negative effects on the environment and therefore governmental policies aim at a reduction of the use of herbicides.
  • policy documents have been drafted in which farmers are being asked to achieve substantial reductions in the use of herbicides.
  • a further problem related to water evaporation is drying out of the soil in pots during dry or hot weather. Such problems may be encountered in the horticultural industry, in the production of pot plants for in-house or garden use and at a consumer level.
  • the second object of the invention is therefore to provide a method for preventing or inhibiting evaporation of water from soils and other substrates for plant growth.
  • Plant-parasitic nematodes are a further major threat to agriculture world wide. Almost all crops are attacked by plant-parasitic nematodes. Damage estimates range from 5-25 % yield reduction, but 100 % losses occur locally, due to plant death or quality loss.
  • Nematodes are conventionally managed by crop rotation and chemical control. For many ne atode problems crop rotation is not very efficient, due to the large host range of the nematodes and the lack of suitable resistant crops or plant varieties. Chemical control often uses compounds that are dangerous to the environment and the user. Many effective nematicides have been banned due to these risks.
  • Lignosulphonates are products resulting from the pulping process of trees. They are separated from the cellulose fibers of the wood where they acts as a binder between the fibers. LS are isolated as a mixture of molecules that are typical repeating monomers of the formula [CH 2 OHCHCH(OH) 05 (S0 3 ) 05 C 6 H 3 0(CH 3 0) ] n .
  • the invention according to a first aspect thereof relates to a method for controlling weed growth in crops, which method comprises the formation of a top layer on the soil in which the crop is growing, which top layer has a mechanical resistance that exceeds the germinal force of the germinating weed, which top layer is formed by application of lignosulphonates on or in the soil.
  • the top layer can be either formed after emergence of the crop plants from the soil or before sowing. In the latter case the layer has a mechanical strength that prevents or inhibits the emergence of weed plants but allows the emergence of crop plants.
  • the amount of lignosulphonates applied to the soil after emergence of the plants is 60 to 2000, preferably 300 to 1200, more preferably 300 to 900, most preferably 480 g/m 2 .
  • the amount of lignosulphonates applied to the soil is 60 to 2000, preferably 300 to 1200, more preferably 300 to 900, most preferably 600 g/m 2 .
  • a further aspect of the invention relates to a method for reducing the evaporation of water from soils, in particular sandy soils, which method comprises the formation of a top layer on the soil in which the crop is growing by application of lignosulphonates on or in the soil.
  • the amount of lignosulphonates applied to the soil is 60 to 2400, preferably 300 to 2200, more preferably 800 to 2000, most preferably 1800 g/m 2 .
  • the top layer thus formed closes off the soil and inhibits or prevents the evaporation of water thereunder.
  • the invention further provides a method for immunizing plants against microorganisms, which method comprises application to the plant of a solution of lignosulphonates.
  • the lignosulphonates may be combined with one or more fungicides to obtain an enhanced or synergistic effect.
  • a method for the protection of plants against nematodes comprising the application of lignosulphonates in the substrate in which the plant is growing.
  • Lignosulphonates will usually be applied against nematodes in soil or in artificial substrates. The most efficient application will be in the situation where plant establishment and the first period of growth is important for final yield. Specific situations in which lignosulphonates can be used are described hereinbelow. These examples are however not intended as limitations.
  • Tulip is sensitive to tobacco rattle virus transmission only for a limited period after planting and the strong effect of lignosulphonates on the vector nematode Paratrichodorus teres shows that virus transmission will be inhibited.
  • a further application of lignosulphonates is for amelioration of replant problems. Replant problems are often caused by nematodes, that attack young trees or shrubs that are replanted at a site were the same species was growing before. Addition of lignosulphonate slurry in the plant hole will inhibit nematode (Pratylenchus, Meloidogyne and others) attack and give the young plant a better chance of establishing a vital root system.
  • Replanting can take place in soil but also in other substrates, such as potting soil, vermiculite, coconut fibers etc. These substrates can be pre-treated with the lignosulphonates. Such pre-treated substrates for plant growth are also part of this invention.
  • the invention furthermore relates to the use of lignosulphonates in the applications described above and to compositions that contain lignosulphonates and are intended for the above identified use.
  • compositions comprise lignosulphonates in an amount to meet the required goal as described above.
  • An optimal amount lies usually around 1 kg/m 2 .
  • the compositions take usually the form of a solution that can be sprayed.
  • Such solution contains about 10-55%, preferably around 30% LS.
  • LS does usually not dissolve above an amount of 55% without additional measures.
  • lignosulphonates is used to indicate either a mixture or isolated lignosulphonate molecules.
  • Crude mixtures still contain 5 to 10% reducing sugars that may lead to stickiness of plant parts, such as leaves, when a solution thereof is sprayed onto or otherwise applied to the plant.
  • Figure 2 Effect of LS and EupareenTM on infection of Botrytis cinera spores on tomato leaves. Values are averages ⁇ SD.
  • Figure 3 Experiments on the effect of Ca- lignosulphonates on the attack of plants by various phytoparasitic nematodes.
  • Figure 4 Attractiveness of Ca-lignosulphonates and NH 4 -lignosulphonates to Pratylenchus penetrans. Presented is percentage nematodes preferring the test solution to the control. Control was demineralised water. The experiment had 5 replications.
  • the spores were treated before application as described by Benito et al . (1998) "Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinera" . In: European Journal of Plant Pathology 104: pp 207-220.
  • the spores were applied (pipet) in duplicate on five leaf segments of two leaves per plant. Eight plants were used per treatment.
  • Pratylenchus penetrans is an endoparasitic nematode, feeding inside the plant roots, whereas Paratrichodorus teres is ectoparasitic, feeding on the outside of the plant roots.
  • Two-week old petunia plans were transferred to 150-ml pots filled with fine dune sand.
  • the pots were placed in a growth cabinet at 15 °C, 80 % relative humidity and provided with water through a watering blanket. The water potential was 1733 Pa. See Figure 3.
  • a Ca-lignosulphonate solution was added in a dose of 10 g (dry content) per Kg soil.
  • the Ca- lignosulphonates were suspended in water (25 g per 100 ml) and pipetted into two 4-cm deep holes next to the stem of the plant.
  • the number of Paratrichodorus teres individuals were counted and the feeding state was assessed by estimation of the percentage of the gut filled with food.
  • the animals were classified as well fed (gut >50 % full) and not well fed (gut ⁇ 50 % full) .
  • the control of the experiment had the same set-up as the treatment with Ca- lignosulphonates, except that instead of the lignosulphonates a corresponding volume of water was added to the pots.
  • the experiment had 4 replicates.
  • the results of the experiment were tested for statistical significance with analysis of variance, using the Genstat V statistical software. Results of experiment A:
  • Two-week old leek plants were transferred into 150-ml pots filled with a mixture of coarse sand and potting soil (1 : 4 v/v) . There were seven plants per pot. After four days a Ca-lignosulphonate solution was added in a dose of 10 g (dry content) per Kg soil. The Ca-lignosulphonates were suspended in water (25 g per 100 ml) and pipetted into two 4-cm deep holes next to the stem of the plant. Three days after addition of the Ca- lignosulphonates 500 individuals (adults and juveniles mixed) of Pratylenchus penetrans were added, suspended in 5 ml of water. The nematodes were pipetted into two holes of 4 cm deep, next to the plants.
  • the control of the experiment had the same setup as the treatment with Ca-lignosulphonates, except that instead of the lignosulphonates a corresponding volume of water was added to the pots.
  • the experiment had 5 replicates.
  • the results of the experiment were tested for statistical significance with analysis of variance, using the Genstat V statistical software.
  • Experiment B was performed under the same conditions as experiment A (see above) and at the same time.
  • the experiment was carried out in 5-cm plastic Petri dishes, without notches.
  • the agar used was technical agar no. 3, Oxoid, at a concentration of 15 g/1.
  • the agar is dispersed in demineralised water and autoclaved for 20 minutes at 120°C. Two ml of agar are dispensed per Petri dish. After cooling of the agar, the nematodes are placed in the middle of the agar dish, while the substances tested are spotted on 2 cm distance from the center. The nematodes (about 100 individuals per sample, juvenile and adult stages mixed) are dispensed in a droplet of 50 ⁇ l tap water.
  • test solution added was 25 ⁇ l of Ca- or NH 4 -1ignosulphonates at a concentration of 5 g/1.
  • the moment of drying of the droplets is considered the starting time of the experiment.
  • the Petri dishes were placed in a plastic box, lined with wet filter paper to ensure 100 % relative air humidity and to prevent drying out of the agar.
  • the boxes with Petri dishes were stored in an incubator at 20°C. Nematode activity was recorded by counting the number of nematodes in a radius of 1 cm from the spot were the test solutions were applied. Counts were made at 2, 8 and 23 hr after start of the experiment.
  • Attractiveness of the test solution was expressed as percentage of the nematodes preferring the test solution to the control. Only nematodes inside the test sections of the agar dish are accounted for in this measure. Non-mobile nematodes at the point of nematode application or active nematodes outside the test sections are not taken into account. The following formula to calculate attractiveness was used:
  • Attractiveness (# nema in section 1 / (# nema in section 2 + # nema in section 1) ) x 100, In which section 1 is the treated section and section 2 is the control section. Attractiveness is expressed as percentage: a value of 50 % means no effect (test solution and control equally attractive) . For statistical testing analysis of variance was performed on log- transformed counts. The experiment had 5 replicates.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne l'utilisation de lignosulphonates pour protéger les plantes. Les lignosulphonates possèdent une variété d'applications, telles que la formation d'une couche de recouvrement sur le sol dans lequel poussent les plantes, de façon à inhiber l'apparition d'herbes, ou la réduction de l'évaporation de l'eau du sol. Lesdits lignosulphonates trouvent également une application dans l'induction de défenses des plantes et dans la lutte contre les nématodes pathogènes des plantes.
PCT/EP2000/011529 1999-11-17 2000-11-17 Utilisation de lignosulphonates pour proteger les plantes Ceased WO2001035747A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00993103A EP1229794A2 (fr) 1999-11-17 2000-11-17 Utilisation de lignosulphonates pour proteger les plantes
AU28366/01A AU2836601A (en) 1999-11-17 2000-11-17 Use of lignosulphonates for plant protection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99203847 1999-11-17
EP99203847.1 1999-11-17

Publications (2)

Publication Number Publication Date
WO2001035747A2 true WO2001035747A2 (fr) 2001-05-25
WO2001035747A3 WO2001035747A3 (fr) 2001-11-15

Family

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PCT/EP2000/011529 Ceased WO2001035747A2 (fr) 1999-11-17 2000-11-17 Utilisation de lignosulphonates pour proteger les plantes

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EP (1) EP1229794A2 (fr)
AU (1) AU2836601A (fr)
WO (1) WO2001035747A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003053883A1 (fr) 2001-12-20 2003-07-03 Basf Aktiengesellschaft Procede pour ameliorer la croissance vegetale par application d'un melange de soufre et d'agent complexant
WO2004067699A3 (fr) * 2003-01-27 2005-01-27 Plant Res Int Bv Compositions comprenant des lignosulfonates destinees a la protection et a l'amelioration des cultures
WO2008007100A3 (fr) * 2006-07-13 2008-10-02 Univ Lancaster Améliorations de la protection des plantes
EP4609869A1 (fr) * 2024-03-01 2025-09-03 Freie Universität Berlin Lignine sulfatée destinée à être utilisée dans l'absorption de pathogènes, dans la purification de l'eau, en tant qu'hydrogel, en tant que médicament, en tant que support de médicament et pour le remplacement de mucus

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3341318A (en) * 1964-12-24 1967-09-12 Arizona Agrochemical Corp Agricultural mulch and herbicidal composition and method
DE4404860A1 (de) * 1994-02-18 1995-08-31 Zschiegner Hans Joachim Dr Verfahren zur Entwicklung und zum Einsatz des Pflanzenstärkungsmittels PHYTO-VITAL
AU3373395A (en) * 1994-08-29 1996-03-22 Thomas T. Yamashita Control of soil borne pests and pathogens
JP4373505B2 (ja) * 1997-05-26 2009-11-25 バイエルクロップサイエンス株式会社 顆粒状水和剤
US6833359B1 (en) * 1998-03-12 2004-12-21 Earth Alive Resources Inc. Method for treating soil for the control of soil borne plant pathogens
JP3856561B2 (ja) * 1998-04-15 2006-12-13 三井化学株式会社 植物病害防除剤組成物
AU5186999A (en) * 1998-08-17 2000-03-06 Earth Alive Resources Inc. Soil treatment method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BENITO ET AL.: "Fungal and Plant Gene Expression During Synchronized Infection of Tomato Leaves by Botrytis Cinera", EUROPEAN JOURNAL OF PLANT PATHOLOGY, vol. 104, 1998, pages 207 - 220
LYON ET AL.: "Novel Disease Control Compounds: The Potential to "Immunize" Plants Against Infection", PLANT PATHOLOGY, vol. 44, 1995, pages 407 - 427

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003053883A1 (fr) 2001-12-20 2003-07-03 Basf Aktiengesellschaft Procede pour ameliorer la croissance vegetale par application d'un melange de soufre et d'agent complexant
US7530196B2 (en) 2001-12-20 2009-05-12 Basf Aktiengesellschaft Method for improving plant growth by application of a mixture of sulfur and complexing agent
WO2004067699A3 (fr) * 2003-01-27 2005-01-27 Plant Res Int Bv Compositions comprenant des lignosulfonates destinees a la protection et a l'amelioration des cultures
WO2008007100A3 (fr) * 2006-07-13 2008-10-02 Univ Lancaster Améliorations de la protection des plantes
US8115053B2 (en) 2006-07-13 2012-02-14 Plant Bioscience Limited Plant protection
AU2007274083B2 (en) * 2006-07-13 2012-12-20 Plant Bioscience Limited Improvements in and relating to plant protection
US8507756B2 (en) 2006-07-13 2013-08-13 Plant Bioscience Limited Plant protection
EP4609869A1 (fr) * 2024-03-01 2025-09-03 Freie Universität Berlin Lignine sulfatée destinée à être utilisée dans l'absorption de pathogènes, dans la purification de l'eau, en tant qu'hydrogel, en tant que médicament, en tant que support de médicament et pour le remplacement de mucus

Also Published As

Publication number Publication date
EP1229794A2 (fr) 2002-08-14
AU2836601A (en) 2001-05-30
WO2001035747A3 (fr) 2001-11-15

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