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WO2001027318A3 - Reverse transcriptase assay - Google Patents

Reverse transcriptase assay Download PDF

Info

Publication number
WO2001027318A3
WO2001027318A3 PCT/GB2000/003880 GB0003880W WO0127318A3 WO 2001027318 A3 WO2001027318 A3 WO 2001027318A3 GB 0003880 W GB0003880 W GB 0003880W WO 0127318 A3 WO0127318 A3 WO 0127318A3
Authority
WO
WIPO (PCT)
Prior art keywords
reverse transcriptase
transcriptase assay
detection
activity
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/003880
Other languages
French (fr)
Other versions
WO2001027318A2 (en
Inventor
Archibald Lovatt
Iain Doherty
Kenneth Thomas Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quip Technology Ltd
Original Assignee
Quip Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quip Technology Ltd filed Critical Quip Technology Ltd
Priority to EP00966304A priority Critical patent/EP1220951A2/en
Priority to AU76748/00A priority patent/AU7674800A/en
Publication of WO2001027318A2 publication Critical patent/WO2001027318A2/en
Publication of WO2001027318A3 publication Critical patent/WO2001027318A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for the detection of a reverse transcriptase (RT) activity, in particular porcine endogenous retrovirus, in a test sample is disclosed. Also provided is a kit for use in the detection of reverse transcriptase activity in a sample.
PCT/GB2000/003880 1999-10-09 2000-10-09 Reverse transcriptase assay Ceased WO2001027318A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00966304A EP1220951A2 (en) 1999-10-09 2000-10-09 Reverse transcriptase assay
AU76748/00A AU7674800A (en) 1999-10-09 2000-10-09 Reverse transcriptase assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9923846.1A GB9923846D0 (en) 1999-10-09 1999-10-09 Reverse transcriptase assay
GB9923846.1 1999-10-09

Publications (2)

Publication Number Publication Date
WO2001027318A2 WO2001027318A2 (en) 2001-04-19
WO2001027318A3 true WO2001027318A3 (en) 2001-10-18

Family

ID=10862379

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003880 Ceased WO2001027318A2 (en) 1999-10-09 2000-10-09 Reverse transcriptase assay

Country Status (4)

Country Link
EP (1) EP1220951A2 (en)
AU (1) AU7674800A (en)
GB (1) GB9923846D0 (en)
WO (1) WO2001027318A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1234052A2 (en) * 1999-11-29 2002-08-28 Glaxo Group Limited Energy-transfer assay for polynucleic acid polymerases
EP1641938A2 (en) * 2003-06-30 2006-04-05 AstraZeneca AB Nucleic acid polymerase fluorescence assays
EP3284831B1 (en) 2010-04-16 2019-07-24 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US11193176B2 (en) * 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
EP3245306A4 (en) 2015-01-16 2018-09-05 Takeda Vaccines, Inc. Detection of particle-contained reverse transcriptase activity
CN116694740A (en) * 2018-08-08 2023-09-05 加利福尼亚大学董事会 Compositions and methods for ordered and continuous complementary DNA (cDNA) synthesis across non-contiguous templates
CN116064722A (en) * 2022-11-14 2023-05-05 翌圣生物科技(上海)股份有限公司 A kit and detection method for detecting reverse transcriptase activity

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040167A1 (en) * 1996-04-19 1997-10-30 Q-One Biotech Ltd. Porcine retrovirus
US5807669A (en) * 1992-05-11 1998-09-15 Schuepbach; Joerg Process for the detection of reverse transcriptase
US5817457A (en) * 1996-02-07 1998-10-06 Ma Bioservices, Inc. Methods and kits for detecting viral reverse transcriptase activity in a sample using an acidic pH or an elevated temperature

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807669A (en) * 1992-05-11 1998-09-15 Schuepbach; Joerg Process for the detection of reverse transcriptase
US5817457A (en) * 1996-02-07 1998-10-06 Ma Bioservices, Inc. Methods and kits for detecting viral reverse transcriptase activity in a sample using an acidic pH or an elevated temperature
WO1997040167A1 (en) * 1996-04-19 1997-10-30 Q-One Biotech Ltd. Porcine retrovirus

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ARNOLD BETH A ET AL: "One-step fluorescent probe product-enhanced reverse transcriptase assay.", BIOTECHNIQUES, vol. 25, no. 1, July 1998 (1998-07-01), pages 98 - 100, 102-106, XP002164696, ISSN: 0736-6205 *
LOVATT A ET AL: "High throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods.", JOURNAL OF VIROLOGICAL METHODS, vol. 82, no. 2, October 1999 (1999-10-01), pages 185 - 200, XP000980366, ISSN: 0166-0934 *
LUGERT R ET AL: "Specific suppression of false-positive signals in the product-enhanced reverse transcriptase assay", BIOTECHNIQUES,EATON PUBLISHING, NATICK,US, vol. 20, no. 2, 1996, pages 210 - 216, XP002159247, ISSN: 0736-6205 *
MARTIGNAT L ET AL: "Detection of porcine endogenous retrovirus: Possible involvement in pig islet xenotransplantation.", DIABETES & METABOLISM, vol. 24, no. 5, November 1998 (1998-11-01), pages 434 - 441, XP000992623, ISSN: 1262-3636 *
MARTIN ULRICH ET AL: "Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells.", LANCET (NORTH AMERICAN EDITION), vol. 352, no. 9129, 29 August 1998 (1998-08-29), pages 692 - 694, XP002164697, ISSN: 0099-5355 *
PYRA HALINA ET AL: "Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 91, no. 4, 1994, 1994, pages 1544 - 1548, XP002159245, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
EP1220951A2 (en) 2002-07-10
GB9923846D0 (en) 1999-12-08
WO2001027318A2 (en) 2001-04-19
AU7674800A (en) 2001-04-23

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