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WO2001023608A3 - Hybridization probes which specifically detect strains of the genera microbispora, microtetraspora, nonomuria and planobispora - Google Patents

Hybridization probes which specifically detect strains of the genera microbispora, microtetraspora, nonomuria and planobispora Download PDF

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Publication number
WO2001023608A3
WO2001023608A3 PCT/EP2000/009459 EP0009459W WO0123608A3 WO 2001023608 A3 WO2001023608 A3 WO 2001023608A3 EP 0009459 W EP0009459 W EP 0009459W WO 0123608 A3 WO0123608 A3 WO 0123608A3
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WO
WIPO (PCT)
Prior art keywords
nonomuria
planobispora
microtetraspora
microbispora
genera
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/009459
Other languages
French (fr)
Other versions
WO2001023608A2 (en
Inventor
Olga Genilloud
Rafael P Mellado
Victor Parro
Vicente Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme de Espana SA
Original Assignee
Merck Sharp and Dohme de Espana SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp and Dohme de Espana SA filed Critical Merck Sharp and Dohme de Espana SA
Publication of WO2001023608A2 publication Critical patent/WO2001023608A2/en
Anticipated expiration legal-status Critical
Publication of WO2001023608A3 publication Critical patent/WO2001023608A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method is provided herein which allows for the specific detection of bateria from the genera Microbispora, Microtetraspora, Nonomuria and Planobispora. This method makes use of four specific nucleic acid probes also provided herein that are complementary to a conserved region of the 16S rRNA genes present within said strains. The probes permit rapid detection of the strains in an accurate and reproducible fashion.
PCT/EP2000/009459 1999-09-27 2000-09-25 Hybridization probes which specifically detect strains of the genera microbispora, microtetraspora, nonomuria and planobispora Ceased WO2001023608A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15617199P 1999-09-27 1999-09-27
US60/156,171 1999-09-27

Publications (2)

Publication Number Publication Date
WO2001023608A2 WO2001023608A2 (en) 2001-04-05
WO2001023608A3 true WO2001023608A3 (en) 2003-04-17

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PCT/EP2000/009459 Ceased WO2001023608A2 (en) 1999-09-27 2000-09-25 Hybridization probes which specifically detect strains of the genera microbispora, microtetraspora, nonomuria and planobispora

Country Status (1)

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WO (1) WO2001023608A2 (en)

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US10927416B2 (en) 2014-03-03 2021-02-23 Fundació Institut D'investigació Biomèdica De Girona Dr. Josep Trueta Method for diagnosing colorectal cancer from a human feces sample by quantitative PCR, primers and kit

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US20040121311A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in livestock
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
CA2508726A1 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122096A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
WO2005117270A2 (en) 2004-05-24 2005-12-08 Isis Pharmaceuticals, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8182992B2 (en) 2005-03-03 2012-05-22 Ibis Biosciences, Inc. Compositions for use in identification of adventitious viruses
CA2616281C (en) 2005-07-21 2014-04-22 Isis Pharmaceuticals, Inc. Methods for rapid identification and quantitation of mitochondrial dna variants
CA2663029C (en) 2006-09-14 2016-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
JP5680304B2 (en) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド Rapid forensic DNA analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
WO2010033625A1 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
WO2011008972A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systems for bioagent identification
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification

Citations (5)

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EP0586112A2 (en) * 1992-08-14 1994-03-09 Pharma Gen, S.A. Control of PCR mediated detection of micro-organisms
WO1995013396A2 (en) * 1993-11-11 1995-05-18 U-Gene Research B.V. A method for identifying microorganisms, and aids useful thereof
DE19732086A1 (en) * 1997-07-25 1999-01-28 Univ Leipzig Quantification of eubacteria in competitive polymerase chain reaction
WO1999014361A1 (en) * 1997-09-18 1999-03-25 Merck Sharp & Dohme De Espana, S.A.E. Hybridization probes which differentiate between streptomycetes and maduromycetes
WO1999035285A2 (en) * 1998-01-08 1999-07-15 Merck Sharp & Dohme De Espana, S.A.E. Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes

Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
EP0586112A2 (en) * 1992-08-14 1994-03-09 Pharma Gen, S.A. Control of PCR mediated detection of micro-organisms
WO1995013396A2 (en) * 1993-11-11 1995-05-18 U-Gene Research B.V. A method for identifying microorganisms, and aids useful thereof
DE19732086A1 (en) * 1997-07-25 1999-01-28 Univ Leipzig Quantification of eubacteria in competitive polymerase chain reaction
WO1999014361A1 (en) * 1997-09-18 1999-03-25 Merck Sharp & Dohme De Espana, S.A.E. Hybridization probes which differentiate between streptomycetes and maduromycetes
WO1999035285A2 (en) * 1998-01-08 1999-07-15 Merck Sharp & Dohme De Espana, S.A.E. Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BARRY T ET AL: "THE 16S/23S RIBOSOMAL SPACER REGION AS A TARGET FOR DNA PROBES TO IDENTIFY EUBACTERIA", PCR METHODS & APPLICATIONS,US,COLD SPRING HARBOR LABORATORY PRESS, vol. 1, 1991, pages 51 - 56, XP000609831, ISSN: 1054-9803 *
GIOVANNONI S J ET AL.: "Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells", JOURNAL OF BACTERIOLOGY, vol. 170, no. 2, 1988, pages 720 - 726, XP000991116 *
STACKEBRANDT ET AL: "Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes", APPLIED AND ENVIRONMENTAL MICROBIOLOGY,US,WASHINGTON,DC, vol. 57, no. 5, 1 May 1991 (1991-05-01), pages 1468 - 1477, XP002090717, ISSN: 0099-2240 *
WANG ET AL: "Phylogenetic analysis reveals new relationships among members of the genera Microtetraspora and Microbispora", INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY,US,NEW YORK, NY, vol. 46, no. 3, 1 July 1996 (1996-07-01), pages 658 - 663, XP002090716 *
ZHANG Z ET AL.: "Reclassification of Thermomonospora and Microtetraspora", INTERNATIONAL JOURNAL OD SYSTEMATIC BACTERIOLOGY, vol. 48, 1998, pages 411 - 422, XP000991112 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10927416B2 (en) 2014-03-03 2021-02-23 Fundació Institut D'investigació Biomèdica De Girona Dr. Josep Trueta Method for diagnosing colorectal cancer from a human feces sample by quantitative PCR, primers and kit

Also Published As

Publication number Publication date
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