[go: up one dir, main page]

WO2001019970A3 - Chymotrypsin-free trypsin - Google Patents

Chymotrypsin-free trypsin Download PDF

Info

Publication number
WO2001019970A3
WO2001019970A3 PCT/US2000/020813 US0020813W WO0119970A3 WO 2001019970 A3 WO2001019970 A3 WO 2001019970A3 US 0020813 W US0020813 W US 0020813W WO 0119970 A3 WO0119970 A3 WO 0119970A3
Authority
WO
WIPO (PCT)
Prior art keywords
chymotrypsin
free trypsin
trypsin
free
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/020813
Other languages
French (fr)
Other versions
WO2001019970A2 (en
Inventor
Jose Michael Hanquier
Charles Lee Hershberger
Jeffrey Lynn Larson
Paul Robert Rosteck Jr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Priority to AU70535/00A priority Critical patent/AU7053500A/en
Publication of WO2001019970A2 publication Critical patent/WO2001019970A2/en
Publication of WO2001019970A3 publication Critical patent/WO2001019970A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to recombinantly produced, essentially chymotrypsin-free trypsin and trypsinogen products. The present invention also includes reagents and processes for producing recombinant trypsinogen which can be subsequently activated to form essentially chymotrypsin-free trypsin.
PCT/US2000/020813 1999-09-15 2000-09-05 Chymotrypsin-free trypsin Ceased WO2001019970A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU70535/00A AU7053500A (en) 1999-09-15 2000-09-05 Chymotrypsin-free trypsin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15401999P 1999-09-15 1999-09-15
US60/154,019 1999-09-15

Publications (2)

Publication Number Publication Date
WO2001019970A2 WO2001019970A2 (en) 2001-03-22
WO2001019970A3 true WO2001019970A3 (en) 2001-11-15

Family

ID=22549683

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/020813 Ceased WO2001019970A2 (en) 1999-09-15 2000-09-05 Chymotrypsin-free trypsin

Country Status (4)

Country Link
AR (1) AR025655A1 (en)
AU (1) AU7053500A (en)
PE (1) PE20010596A1 (en)
WO (1) WO2001019970A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
CA2437342C (en) 2001-02-01 2013-08-06 F. Hoffmann-La Roche Ag Method for producing recombinant trypsin
WO2012104099A1 (en) * 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN103966191B (en) * 2013-02-04 2016-01-20 甘李药业股份有限公司 The tryptic preparation method of a kind of recombinant bovine source property
WO2014167574A1 (en) * 2013-03-22 2014-10-16 Biogenomics Limited Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins
EP3221449B1 (en) 2014-11-18 2023-08-30 Merck Sharp & Dohme LLC Process for refolding recombinant chymotrypsin
WO2016081289A1 (en) 2014-11-18 2016-05-26 Merck Sharp & Dohme Corp. Process for producing recombinant trypsin
CN104694522B (en) * 2015-02-16 2018-06-12 中国人民解放军军事医学科学院放射与辐射医学研究所 A kind of preparation method and applications for recombinating acetylation cationic trypsase
KR20220092969A (en) * 2019-11-07 2022-07-04 암젠 인크 High salt loading conditioning during cation exchange chromatography to remove product-related impurities
GB202015618D0 (en) * 2020-10-01 2020-11-18 Ipsen Biopharm Ltd Method for producing beta-trypsin

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077392A (en) * 1988-10-17 1991-12-31 Boehringer Mannheim Gmbh Process for activation of recombinant protein produced by prokaryotes
EP0495398A1 (en) * 1991-01-11 1992-07-22 Roche Diagnostics GmbH Recombinant IgA-protease
EP0597681A1 (en) * 1992-11-13 1994-05-18 Eli Lilly And Company Expression vectors for the bovine trypsin and trypsinogen and host cells transformed therewith
WO1999010503A1 (en) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
WO2000017332A1 (en) * 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077392A (en) * 1988-10-17 1991-12-31 Boehringer Mannheim Gmbh Process for activation of recombinant protein produced by prokaryotes
EP0495398A1 (en) * 1991-01-11 1992-07-22 Roche Diagnostics GmbH Recombinant IgA-protease
EP0597681A1 (en) * 1992-11-13 1994-05-18 Eli Lilly And Company Expression vectors for the bovine trypsin and trypsinogen and host cells transformed therewith
WO1999010503A1 (en) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
WO2000017332A1 (en) * 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HUEROU LE I ET AL: "ISOLATION AND NUCLEOTIDE SEQUENCE OF CDNA CLONE FOR BOVINE PANCREATIC ANIONIC TRYPSINOGEN STRUCTURAL IDENTITY WITHIN THE TRYPSIN FAMILY", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 193, November 1990 (1990-11-01), pages 767 - 773, XP000983599, ISSN: 0014-2956 *
VASQUEZ J R ET AL: "AN EXPRESSION SYSTEM FOR TRYPSIN", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 39, March 1989 (1989-03-01), pages 265 - 276, XP000983672, ISSN: 0730-2312 *

Also Published As

Publication number Publication date
PE20010596A1 (en) 2001-05-23
AR025655A1 (en) 2002-12-11
WO2001019970A2 (en) 2001-03-22
AU7053500A (en) 2001-04-17

Similar Documents

Publication Publication Date Title
LUC00074I2 (en) Method for controlling the activity of immunologically functional molecule
WO2001034646A3 (en) Recombinant gelatins
WO2001004144A3 (en) Fabrication of beta-pleated sheet proteins with specific binding properties
WO2003050202A8 (en) A method of forming a composite
WO2001053312A8 (en) Novel nucleic acids and polypeptides
WO2001053455A3 (en) Novel nucleic acids and polypeptides
AU2001274511A1 (en) Adsorbent, process for producing the same, and applications thereof
EP0665590A3 (en) Microstructure, process for manufacturing thereof and devices incorporating the same.
WO2001075067A3 (en) Novel nucleic acids and polypeptides
WO2001057188A3 (en) Novel nucleic acids and polypeptides
WO2001057190A3 (en) Novel nucleic acids and polypeptides
WO2003011226A3 (en) Products and drug delivery vehicles
WO2002096898A3 (en) Process for the preparation of prostaglandins and analogues thereof
ZA200204312B (en) Laminate and process for its manufacture.
AU2002250858A1 (en) Capsule-in-capsule system and method for the production thereof
AU2001244609A1 (en) Novel protein, dna thereof and process for producing the same
WO2001019970A3 (en) Chymotrypsin-free trypsin
MXPA03010322A (en) Method for producing polyisocyanates.
WO2000022019A3 (en) Coupled block copolymers and process for producing the block copolymers
HK1053116A1 (en) Processes for preparing cilostazol
EP1186658A4 (en) Enzyme liquor and process for producing the same, enzyme preparation, protease preparations and protease-producing bacterium
AU2001289955A1 (en) Base material for preparing vitreous or vitrocrystalline parts. method for preparing the base material and method for manufacturing the parts
WO2003020759A3 (en) A caspase- 8 binding protein, its preparation and use
EP0967286A3 (en) Leucine aminopeptidase gene, recombinant DNA, and process for producing leucine aminopeptidase
AU2001242750A1 (en) Novel protein, process for producing the same and use thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP