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WO2001019853A3 - Cell transfection - Google Patents

Cell transfection Download PDF

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Publication number
WO2001019853A3
WO2001019853A3 PCT/GB2000/003462 GB0003462W WO0119853A3 WO 2001019853 A3 WO2001019853 A3 WO 2001019853A3 GB 0003462 W GB0003462 W GB 0003462W WO 0119853 A3 WO0119853 A3 WO 0119853A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
tissue
cell transfection
transfected
transfected cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/003462
Other languages
French (fr)
Other versions
WO2001019853A2 (en
Inventor
Anthony Peter Hollander
Michael David Barker
Waeh Kafienah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Sheffield
Original Assignee
University of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Sheffield filed Critical University of Sheffield
Priority to AU73003/00A priority Critical patent/AU7300300A/en
Publication of WO2001019853A2 publication Critical patent/WO2001019853A2/en
Publication of WO2001019853A3 publication Critical patent/WO2001019853A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An isolated nucleic acid molecule comprising at least the functional part of the blasticidin resistance (BS) gene, or homologue thereof, linked via a recognition sequence, to at least one selected gene; a method for the preparation thereof, a method for transfecting cells, a transfected cell and tissue comprising a transfected cell, a method for 'knockout', a method for incubating mitotically inactive cells, a therapeutic composition comprising transfected cells or tissue, and the use thereof.
PCT/GB2000/003462 1999-09-11 2000-09-11 Cell transfection Ceased WO2001019853A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU73003/00A AU7300300A (en) 1999-09-11 2000-09-11 Cell transfection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9921418.1 1999-09-11
GBGB9921418.1A GB9921418D0 (en) 1999-09-11 1999-09-11 Cell transfection

Publications (2)

Publication Number Publication Date
WO2001019853A2 WO2001019853A2 (en) 2001-03-22
WO2001019853A3 true WO2001019853A3 (en) 2001-11-15

Family

ID=10860700

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/003462 Ceased WO2001019853A2 (en) 1999-09-11 2000-09-11 Cell transfection

Country Status (3)

Country Link
AU (1) AU7300300A (en)
GB (1) GB9921418D0 (en)
WO (1) WO2001019853A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE555208T1 (en) * 2003-06-13 2012-05-15 Apath Llc REPLICON OF A NEGATIVE STRAND RNA VIRUS

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648838A1 (en) * 1993-10-06 1995-04-19 Amgen Inc. Tissue inhibitor of metalloproteinase type three (TIMP-3)
US5527701A (en) * 1992-08-25 1996-06-18 The Institute Of Physical And Chemical Research Aspergillus terreus blasticidin S deaminase, a blasticidin S deaminase gene, a vector incorporating said gene and a transformant containing said vector
US5693508A (en) * 1994-11-08 1997-12-02 Chang; Lung-Ji Retroviral expression vectors containing MoMLV/CMV-IE/HIV-TAR chimeric long terminal repeats
WO1999028452A1 (en) * 1997-11-28 1999-06-10 Mochida Pharmaceutical Co., Ltd. Matrilysin antisense compounds
WO1999043795A1 (en) * 1998-02-24 1999-09-02 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Trans-somatics with gene transfer into mammary epithelial cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5527701A (en) * 1992-08-25 1996-06-18 The Institute Of Physical And Chemical Research Aspergillus terreus blasticidin S deaminase, a blasticidin S deaminase gene, a vector incorporating said gene and a transformant containing said vector
EP0648838A1 (en) * 1993-10-06 1995-04-19 Amgen Inc. Tissue inhibitor of metalloproteinase type three (TIMP-3)
US5693508A (en) * 1994-11-08 1997-12-02 Chang; Lung-Ji Retroviral expression vectors containing MoMLV/CMV-IE/HIV-TAR chimeric long terminal repeats
WO1999028452A1 (en) * 1997-11-28 1999-06-10 Mochida Pharmaceutical Co., Ltd. Matrilysin antisense compounds
WO1999043795A1 (en) * 1998-02-24 1999-09-02 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Trans-somatics with gene transfer into mammary epithelial cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1 January 1900 (1900-01-01), "PLASMID PAMS WITH HYBRID AMPHOTROPIC/MOLONEY MURINE LEUKEMIA VIRUS, COMPLETE SEQUENCE", XP002168756, Database accession no. AF010170 *
DATABASE EMBL 1 January 1900 (1900-01-01), LOU Y, HOLTZ A: "CLONING VECTOR PIRES1HYG, COMPLETE PLASMID SEQUENCE", XP002168755, Database accession no. U89672 *
DATABASE WPI Section Ch Week 199930, Derwent World Patents Index; Class B04, AN 1999-358127, XP002168757 *
MASAKO ISUMI ET AL: "BLASTICIDIN S-RESISTANCE GENE (BSR): A NOVEL SELECTABLE MARKER FOR MAMMALIAN CELLS", EXPERIMENTAL CELL RESEARCH,US,SAN DIEGO, CA, vol. 197, no. 2, 1 December 1991 (1991-12-01), pages 229 - 233, XP000196772, ISSN: 0014-4827 *
URABE M ET AL: "A novel dicistronic AAV vector using a short IRES segment derived from hepatitis C virus genome", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 200, no. 1-2, 24 October 1997 (1997-10-24), pages 157 - 162, XP004126490, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
GB9921418D0 (en) 1999-11-10
AU7300300A (en) 2001-04-17
WO2001019853A2 (en) 2001-03-22

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