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WO2001018545A3 - Method for identifying binding partners with position-specific arrays - Google Patents

Method for identifying binding partners with position-specific arrays Download PDF

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Publication number
WO2001018545A3
WO2001018545A3 PCT/DE2000/003071 DE0003071W WO0118545A3 WO 2001018545 A3 WO2001018545 A3 WO 2001018545A3 DE 0003071 W DE0003071 W DE 0003071W WO 0118545 A3 WO0118545 A3 WO 0118545A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligat
array
specific
binding partners
identifying binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2000/003071
Other languages
German (de)
French (fr)
Other versions
WO2001018545A2 (en
Inventor
Eberhard Krause
Thomas Bittorf
Jens Schneider-Mergener
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JERINI BIO TOOLS GmbH
SCHNEIDER MERGENER JENS
Original Assignee
JERINI BIO TOOLS GmbH
SCHNEIDER MERGENER JENS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by JERINI BIO TOOLS GmbH, SCHNEIDER MERGENER JENS filed Critical JERINI BIO TOOLS GmbH
Priority to DE10082703T priority Critical patent/DE10082703D2/en
Priority to AU76442/00A priority patent/AU7644200A/en
Priority to HU0203074A priority patent/HUP0203074A2/en
Priority to CA002386607A priority patent/CA2386607A1/en
Priority to PL00353995A priority patent/PL353995A1/en
Priority to JP2001522083A priority patent/JP2003511652A/en
Priority to EP00965834A priority patent/EP1212622A2/en
Publication of WO2001018545A2 publication Critical patent/WO2001018545A2/en
Publication of WO2001018545A3 publication Critical patent/WO2001018545A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed is a method for identifying binding partners with position-specific arrays by means of mass spectrometry. A position-specific ligat array is produced on a carrier material by means of small volumes which are position-specifically mounted and belong to reaction partners. At least two successive reactions are carried out gradually. The position-specific ligat array that is fixed to the carrier material is incubated with a mixture of ligands. Ligands that are not bound to the ligat array are subsequently removed and the ligand/s that is/are bound to the ligat array are further characterised by means of mass spectrometry.
PCT/DE2000/003071 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays Ceased WO2001018545A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE10082703T DE10082703D2 (en) 1999-09-03 2000-09-01 Procedure for identifying binding partners with position-specific arrays (no entry into the national phase)
AU76442/00A AU7644200A (en) 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays
HU0203074A HUP0203074A2 (en) 1999-09-03 2000-09-01 A procedure for identifying binding partners with site-specific arrays
CA002386607A CA2386607A1 (en) 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays
PL00353995A PL353995A1 (en) 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays
JP2001522083A JP2003511652A (en) 1999-09-03 2000-09-01 Method for identifying a binding partner having a position-specific sequence
EP00965834A EP1212622A2 (en) 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19943743.2 1999-09-03
DE19943743A DE19943743C2 (en) 1999-09-03 1999-09-03 Procedure for the identification of binding partners with position-specific arrays

Publications (2)

Publication Number Publication Date
WO2001018545A2 WO2001018545A2 (en) 2001-03-15
WO2001018545A3 true WO2001018545A3 (en) 2001-09-13

Family

ID=7921806

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/003071 Ceased WO2001018545A2 (en) 1999-09-03 2000-09-01 Method for identifying binding partners with position-specific arrays

Country Status (9)

Country Link
EP (1) EP1212622A2 (en)
JP (1) JP2003511652A (en)
AU (1) AU7644200A (en)
CA (1) CA2386607A1 (en)
CZ (1) CZ2002660A3 (en)
DE (2) DE19943743C2 (en)
HU (1) HUP0203074A2 (en)
PL (1) PL353995A1 (en)
WO (1) WO2001018545A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7102005B2 (en) 2002-05-03 2006-09-05 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US7445894B2 (en) 2002-05-03 2008-11-04 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US7635572B2 (en) 2003-06-09 2009-12-22 Life Technologies Corporation Methods for conducting assays for enzyme activity on protein microarrays

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10141464A1 (en) * 2001-08-23 2004-03-04 Genprofile Ag I.Ins. New nucleic acid representing a polymorphism in the 22444 gene, useful for diagnosis and treatment of neuropsychiatric diseases and in drug screening
EP1319954A1 (en) * 2001-12-12 2003-06-18 Centre National de Genotypage Methods for protein analysis using protein capture arrays
US7504364B2 (en) * 2002-03-01 2009-03-17 Receptors Llc Methods of making arrays and artificial receptors
US20030228709A1 (en) * 2002-03-25 2003-12-11 Kozlowski Roland Zbignieiw Arrays
US7469076B2 (en) 2003-09-03 2008-12-23 Receptors Llc Sensors employing combinatorial artificial receptors
EP1613737A4 (en) 2003-03-28 2008-12-03 Receptors Llc Artificial receptors including reversibly immobilized building blocks and methods
US7884052B2 (en) 2004-09-03 2011-02-08 Receptors Llc Combinatorial artificial receptors including tether building blocks on scaffolds
EP1789792A2 (en) 2004-09-11 2007-05-30 Receptors LLC Combinatorial artificial receptors including peptide building blocks

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0742438A2 (en) * 1995-05-10 1996-11-13 Bayer Corporation Screening of combinatorial peptide libraries for selection of peptide ligand useful in affinity purification of target proteins
WO1997043301A2 (en) * 1996-05-10 1997-11-20 Novartis Ag Identification of members of combinatorial libraries by mass spectrometry
WO1999012040A2 (en) * 1997-09-02 1999-03-11 Sequenom, Inc. Mass spectrometric detection of polypeptides
WO1999035502A1 (en) * 1998-01-08 1999-07-15 The University Of Liverpool Use of mass fingerprinting for identification of protein affinity ligands

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040423A (en) * 1990-08-31 2000-03-21 Gsellschaft Fur Biotechnologische Forschung Mbh (Gbf) Process for synthesis of peptides
WO1992009690A2 (en) * 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with altered binding properties

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0742438A2 (en) * 1995-05-10 1996-11-13 Bayer Corporation Screening of combinatorial peptide libraries for selection of peptide ligand useful in affinity purification of target proteins
WO1997043301A2 (en) * 1996-05-10 1997-11-20 Novartis Ag Identification of members of combinatorial libraries by mass spectrometry
WO1999012040A2 (en) * 1997-09-02 1999-03-11 Sequenom, Inc. Mass spectrometric detection of polypeptides
WO1999035502A1 (en) * 1998-01-08 1999-07-15 The University Of Liverpool Use of mass fingerprinting for identification of protein affinity ligands

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
M. A. KELLY ET AL.: "Characterization of SH2-ligand interactions via library affinity selection with mass spectrometric detection.", BIOCHEMISTRY., vol. 35, no. 36, 1996, AMERICAN CHEMICAL SOCIETY. EASTON, PA., US, pages 11747 - 11755, XP002062459, ISSN: 0006-2960 *
P. L. COURCHESNE ET AL.: "Identification of proteins by matrix-assisted laser desorption/ionization mass spectrometry using peptide and fragment ion masses (from "2D Proteome Analysis Protocols, Ed. A. J. Link)", METHODS IN MOLECULAR BIOLOGY, vol. 112, 1999, pages 487 - 511, XP002103063 *
S. R. PENNINGTON ET AL.: "Proteome analysis: from protein characterization to biological function.", TRENDS IN CELL BIOLOGY., vol. 7, April 1997 (1997-04-01), ELSEVIER SCIENCE LTD., XX, pages 168 - 173, XP002103064, ISSN: 0962-8924 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7102005B2 (en) 2002-05-03 2006-09-05 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US7445894B2 (en) 2002-05-03 2008-11-04 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US7635572B2 (en) 2003-06-09 2009-12-22 Life Technologies Corporation Methods for conducting assays for enzyme activity on protein microarrays

Also Published As

Publication number Publication date
JP2003511652A (en) 2003-03-25
CA2386607A1 (en) 2001-03-15
CZ2002660A3 (en) 2002-10-16
DE19943743C2 (en) 2002-02-07
DE10082703D2 (en) 2002-02-14
HUP0203074A2 (en) 2002-12-28
PL353995A1 (en) 2003-12-15
WO2001018545A2 (en) 2001-03-15
AU7644200A (en) 2001-04-10
DE19943743A1 (en) 2001-03-15
EP1212622A2 (en) 2002-06-12

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