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WO2001012847A3 - Isolation of cells from forensic samples for dna-typing - Google Patents

Isolation of cells from forensic samples for dna-typing Download PDF

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Publication number
WO2001012847A3
WO2001012847A3 PCT/EP2000/007771 EP0007771W WO0112847A3 WO 2001012847 A3 WO2001012847 A3 WO 2001012847A3 EP 0007771 W EP0007771 W EP 0007771W WO 0112847 A3 WO0112847 A3 WO 0112847A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific
cells
present
relates
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2000/007771
Other languages
French (fr)
Other versions
WO2001012847A2 (en
Inventor
Den Eeckhout Elfride Van
Dieter Deforce
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiteit Gent
Original Assignee
Universiteit Gent
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiteit Gent filed Critical Universiteit Gent
Priority to AU67012/00A priority Critical patent/AU6701200A/en
Publication of WO2001012847A2 publication Critical patent/WO2001012847A2/en
Publication of WO2001012847A3 publication Critical patent/WO2001012847A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2833—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a species-specific, cell-type- or individual-specific molecule for use in a method for isolating cells from a forensic sample with said species-specific, cell-type-specific or individual-specific molecule being preferably bound to a solid phase. More particularly, said molecule is a monoclonal or a polyclonal antibody. The present invention also relates to a method for isolating cells from a forensic sample by capturing the cells from said sample using a species-specific, cell-type-specific or individual-specific molecule. The present invention also relates to methods for preparing and analysing DNA from a forensic sample. The present invention also relates to methods for isolating specific cells from mixtures of cells present in a forensic sample.
PCT/EP2000/007771 1999-08-11 2000-08-10 Isolation of cells from forensic samples for dna-typing Ceased WO2001012847A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU67012/00A AU6701200A (en) 1999-08-11 2000-08-10 Isolation of cells from forensic samples for dna-typing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP99870173 1999-08-11
EP99870173.4 1999-08-11
US19929400P 2000-04-24 2000-04-24
US60/199,294 2000-04-24

Publications (2)

Publication Number Publication Date
WO2001012847A2 WO2001012847A2 (en) 2001-02-22
WO2001012847A3 true WO2001012847A3 (en) 2001-08-30

Family

ID=26153851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/007771 Ceased WO2001012847A2 (en) 1999-08-11 2000-08-10 Isolation of cells from forensic samples for dna-typing

Country Status (2)

Country Link
AU (1) AU6701200A (en)
WO (1) WO2001012847A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7320891B2 (en) 2004-09-10 2008-01-22 Promega Corporation Methods and kits for isolating sperm cells
US8993292B2 (en) * 2007-01-16 2015-03-31 Applied Biosystems Llc Methods and systems for differential extraction
CN103592432B (en) * 2013-10-15 2015-06-03 重庆市公安局 Method for separating sperm in sperm and epithelial cell mixed stain by using immunological magnetic beads

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0323829A2 (en) * 1988-01-04 1989-07-12 E.I. Du Pont De Nemours And Company Multiple stage affinity process for isolation of specific cells from a cell mixture
WO1992013969A1 (en) * 1991-01-31 1992-08-20 Baylor College Of Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0323829A2 (en) * 1988-01-04 1989-07-12 E.I. Du Pont De Nemours And Company Multiple stage affinity process for isolation of specific cells from a cell mixture
WO1992013969A1 (en) * 1991-01-31 1992-08-20 Baylor College Of Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
BISHARA A AND BRAUTBAR C: "A sensitive and specific ELISA using monoclonal capture antibodies for the detection of the HLA antigens in blood stains", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 120, 1989, pages 99 - 103, XP002128643 *
BISHARA A AND BRAUTBAR C: "Detection and identification of HLA antigens in dried spleens by means of enzyme linked immunosorbent assay (ELISA)", FORENSIC SCIENCE INTERNATIONAL, vol. 45, 1990, pages 97 - 105, XP000869791 *
BUTLER J M ET AL: "Forensic applications of mitochondrial DNA", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 16, no. 4, pages 158-162, XP004112301 *
CATTANEO C ET AL.: "Comparison of three DNA extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences", JOURNAL OF FORENSIC SCIENCE, vol. 42, no. 6, 1997, pages 1126 - 1135, XP000869687 *
DIXON S C ET AL.: "Methods for extracting and amplifying genomic DNA isolated from frozen serum", NATURE BIOTECHNOLOGY, vol. 16, 1998, pages 91 - 94, XP002128645 *
GEE A P ET AL.: "T lymphozyte depletion of human micropheral blood and bone marrow using monoclonal antibodies and magnetic microspheres", BONE MARROW TRANSPLANTATION, vol. 2, no. 2, 1987, pages 155 - 163, XP000869902 *
GREENSPOON S A ET AL.: "QIAamp spin columns as a method of DNA isolation for forensic casework", JOURNAL OF FORENSIC SCIENCES, vol. 43, no. 5, 1998, pages 1024 - 1030, XP000974645 *
HANSEN T AND HANNESTAD K: "Direct HLA typing by rosetting with immunomagnetic beads coated with specific antibodies", JOURNAL OF IMMUNOGENETICS, vol. 16, no. 2, 1989, pages 137 - 139, XP000869908 *
MATTSSON L ET AL.: "Isolation of cell populations from arterial tissue, using monoclonal antibodies and magnetic microspheres", ATHEROSCLEROSIS, vol. 89, no. 1, 1991, pages 25 - 34, XP002128644 *
VARTDAL F ET AL.: "HLA class I and II typing using cells positively selected from blood by immunomagnetic isolation - a fast and reliable technique", TISSUE ANTIGENS, vol. 28, 1986, pages 301 - 312, XP000867222 *
WAYE J S ET AL: "A SIMPLE AND SENSITIVE METHOD FOR QUANTIFYING HUMAN GENOMIC DNA IN FORENSIC SPECIMEN EXTRACTS", BIOTECHNIQUES,US,NATICK, MA, vol. 7, no. 8, pages 852-855, XP000652990 *
ZIEGLER B L ET AL.: "Analysis of gene expression in small numbers of purified hemopoietic progenitor cells by RT-PCR", STEM CELLS, vol. 13, no. Sup1, 1995, pages 106 - 116, XP000870268 *

Also Published As

Publication number Publication date
WO2001012847A2 (en) 2001-02-22
AU6701200A (en) 2001-03-13

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