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WO2001006008A3 - Multiplexed strand displacement for nucleic acid determinations - Google Patents

Multiplexed strand displacement for nucleic acid determinations Download PDF

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Publication number
WO2001006008A3
WO2001006008A3 PCT/US2000/018301 US0018301W WO0106008A3 WO 2001006008 A3 WO2001006008 A3 WO 2001006008A3 US 0018301 W US0018301 W US 0018301W WO 0106008 A3 WO0106008 A3 WO 0106008A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
determinations
multiplexed
oligonucleotides
release
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/018301
Other languages
French (fr)
Other versions
WO2001006008A2 (en
Inventor
Sharat Singh
Anita Inamdar
Edwin F Ullman
Liching Cao
David Albagli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monogram Biosciences Inc
Original Assignee
Aclara Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc filed Critical Aclara Biosciences Inc
Priority to EP00948570A priority Critical patent/EP1200624A2/en
Priority to CA002379749A priority patent/CA2379749A1/en
Priority to JP2001511216A priority patent/JP2003505036A/en
Publication of WO2001006008A2 publication Critical patent/WO2001006008A2/en
Publication of WO2001006008A3 publication Critical patent/WO2001006008A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Multiplexed determinations of large numbers of events are achieved in an accurate and simple manner by using a combination of different oligonucleotides, which serve for both capture and release from a support and strand displacement oligonucleotides for sequential release of groups of oligonucleotides. Also included, as part of the oligonucleotide reagents may be identifiers, which serve to identify a particular characteristic. The method is illustrated using primers for initiation of chain extension joined to or serving as the capture/release sequence, where the extended primer has an identifier. After extending the primer, the extended primers are captured and independently released and the released extended primers assayed. The subject method finds application for nucleic acid sequence determinations, single nucleotide polymorphism determinations, identification of nucleic acid fragments, and the like.
PCT/US2000/018301 1999-07-16 2000-06-30 Multiplexed strand displacement for nucleic acid determinations Ceased WO2001006008A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00948570A EP1200624A2 (en) 1999-07-16 2000-06-30 Multiplexed strand displacement for nucleic acid determinations
CA002379749A CA2379749A1 (en) 1999-07-16 2000-06-30 Multiplexed strand displacement for nucleic acid determinations
JP2001511216A JP2003505036A (en) 1999-07-16 2000-06-30 Multiple strand displacement for nucleic acid determination

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35462999A 1999-07-16 1999-07-16
US09/354,629 1999-07-16

Publications (2)

Publication Number Publication Date
WO2001006008A2 WO2001006008A2 (en) 2001-01-25
WO2001006008A3 true WO2001006008A3 (en) 2001-07-12

Family

ID=23394232

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/018301 Ceased WO2001006008A2 (en) 1999-07-16 2000-06-30 Multiplexed strand displacement for nucleic acid determinations

Country Status (4)

Country Link
EP (1) EP1200624A2 (en)
JP (1) JP2003505036A (en)
CA (1) CA2379749A1 (en)
WO (1) WO2001006008A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002029109A2 (en) * 2000-10-05 2002-04-11 Aclara Biosciences, Inc. Multiplexed differential displacement for nucleic acid determinations
JP4839051B2 (en) * 2004-10-08 2011-12-14 シスメックス株式会社 Method for detecting a test substance using a nucleic acid probe
DE602005018316D1 (en) * 2004-10-08 2010-01-28 Sysmex Corp A method of detecting a target substance using a nucleic acid probe
WO2024123917A1 (en) * 2022-12-07 2024-06-13 Illumina, Inc. Monoclonal clustering using double stranded dna size exclusion with patterned seeding

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003911A1 (en) * 1985-12-17 1987-07-02 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex
EP0269764A1 (en) * 1986-12-01 1988-06-08 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
WO1998014610A2 (en) * 1996-10-04 1998-04-09 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
EP0871774A1 (en) * 1995-07-25 1998-10-21 University Of Strathclyde Detection of nucleic acids and nucleic acid units
WO1999024612A2 (en) * 1997-11-06 1999-05-20 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003911A1 (en) * 1985-12-17 1987-07-02 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
EP0269764A1 (en) * 1986-12-01 1988-06-08 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
EP0871774A1 (en) * 1995-07-25 1998-10-21 University Of Strathclyde Detection of nucleic acids and nucleic acid units
WO1998014610A2 (en) * 1996-10-04 1998-04-09 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
WO1999024612A2 (en) * 1997-11-06 1999-05-20 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WOLCOTT M J: "ADVANCES IN NUCLEIC ACID-BASED DETECTION METHODS", CLINICAL MICROBIOLOGY REVIEWS,US,WASHINGTON, DC, vol. 5, no. 4, 1 October 1992 (1992-10-01), pages 370 - 386, XP000606216, ISSN: 0893-8512 *

Also Published As

Publication number Publication date
WO2001006008A2 (en) 2001-01-25
JP2003505036A (en) 2003-02-12
EP1200624A2 (en) 2002-05-02
CA2379749A1 (en) 2001-01-25

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