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WO2001092888A3 - Procede de criblage et d'isolement de microorganismes, en particulier de cellules procaryotes et eucaryotes, portant un antigene - Google Patents

Procede de criblage et d'isolement de microorganismes, en particulier de cellules procaryotes et eucaryotes, portant un antigene Download PDF

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Publication number
WO2001092888A3
WO2001092888A3 PCT/EP2001/006123 EP0106123W WO0192888A3 WO 2001092888 A3 WO2001092888 A3 WO 2001092888A3 EP 0106123 W EP0106123 W EP 0106123W WO 0192888 A3 WO0192888 A3 WO 0192888A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigen
microorganisms
suspension
antibody
antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/006123
Other languages
English (en)
Other versions
WO2001092888A2 (fr
Inventor
Markus Hanner
Markus Horky
Gabain Alexander Von
Ahsen Uwe Von
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CISTEM Biotechnologies GmbH
Original Assignee
CISTEM Biotechnologies GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CISTEM Biotechnologies GmbH filed Critical CISTEM Biotechnologies GmbH
Priority to AU8382501A priority Critical patent/AU8382501A/xx
Publication of WO2001092888A2 publication Critical patent/WO2001092888A2/fr
Publication of WO2001092888A3 publication Critical patent/WO2001092888A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne un procédé de criblage et d'isolement de microorganismes, en particulier de cellules procaryotes et eucaryotes, portant un antigène, lequel procédé est caractérisé par les étapes suivantes : on forme une suspension d'une multiplicité de microorganismes viables contenant un ou plusieurs microorganismes, en particulier de cellules procaryotes et eucaryotes, portant au moins un type d'antigène à leur surface, on met en contact la suspension de microorganismes précitée avec une solution contenant des anticorps qui lient au moins un type d'antigène, on fait incuber la solution précitée avec les anticorps précités afin de permettre aux anticorps de lier l'antigène/les antigènes, on met en contact la suspension précitée de microorganismes portant les complexes anticorps/antigène à leur surface avec des molécules C1q afin de permettre aux molécules C1q de lier les complexes anticorps/antigène placés en surface et d'isoler les microorganismes viables, en particulier les cellules procaryotes et eucaryotes, portant des molécules C1q liées à un complexe anticorps/antigène à leur surface.
PCT/EP2001/006123 2000-05-31 2001-05-30 Procede de criblage et d'isolement de microorganismes, en particulier de cellules procaryotes et eucaryotes, portant un antigene Ceased WO2001092888A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU8382501A AU8382501A (en) 2000-05-31 2001-05-30 A method for screening and isolating microorganisms, especially prokaryotic and eukaryotic cells, displaying an antigen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT9612000A AT409801B (de) 2000-05-31 2000-05-31 Verfahren zum screenen und isolieren von mikroorganismen, insbesondere prokaryontischer und eukaryontischer zellen, die ein antigen präsentieren
ATA961/2000 2000-05-31

Publications (2)

Publication Number Publication Date
WO2001092888A2 WO2001092888A2 (fr) 2001-12-06
WO2001092888A3 true WO2001092888A3 (fr) 2002-07-04

Family

ID=3683572

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006123 Ceased WO2001092888A2 (fr) 2000-05-31 2001-05-30 Procede de criblage et d'isolement de microorganismes, en particulier de cellules procaryotes et eucaryotes, portant un antigene

Country Status (3)

Country Link
AT (1) AT409801B (fr)
AU (1) AU8382501A (fr)
WO (1) WO2001092888A2 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062935A (en) * 1974-05-20 1977-12-13 Technicon Instruments Corporation Immunoassay involving the binding of RF to the antigen-antibody complex
US4307190A (en) * 1978-10-30 1981-12-22 Technicon Instruments Corporation Immunoassay using ascitic fluid
US4882423A (en) * 1984-10-02 1989-11-21 Calpis Food Industry Substance-conjugated complement component C1q
US5035995A (en) * 1984-10-02 1991-07-30 Calpis Food Industry Co., Ltd. Test method involving substance-conjugated complement component C1q
JPH03233358A (ja) * 1990-02-08 1991-10-17 Internatl Reagents Corp 抗原または抗体の高感度測定法
WO1996021675A2 (fr) * 1995-01-13 1996-07-18 Innogenetics N.V. Capture de micro-organismes a l'aide de constituants du complement

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551435A (en) * 1983-08-24 1985-11-05 Immunicon, Inc. Selective removal of immunospecifically recognizable substances from solution
EP0152254A3 (fr) * 1984-02-06 1986-08-06 E.I. Du Pont De Nemours And Company Immunoessai à base d'un support chromogénique utilisant des composants complémentaires marqués
EP0177023A3 (fr) * 1984-10-02 1987-08-12 The Calpis Food Industry Co., Ltd. Composant de complément C1q lié à une substance
ATE77700T1 (de) * 1986-03-10 1992-07-15 Imre Corp Immunkomplextestverfahren.
US4925788A (en) * 1986-10-24 1990-05-15 Immunicon Corporation Immunoassay system and procedure based on precipitin-like interaction between immune complex and Clq or other non-immunospecific factor
US6013532A (en) * 1990-09-26 2000-01-11 Immunivest Corporation Methods for magnetic immobilization and manipulation of cells
US5466574A (en) * 1991-03-25 1995-11-14 Immunivest Corporation Apparatus and methods for magnetic separation featuring external magnetic means

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062935A (en) * 1974-05-20 1977-12-13 Technicon Instruments Corporation Immunoassay involving the binding of RF to the antigen-antibody complex
US4307190A (en) * 1978-10-30 1981-12-22 Technicon Instruments Corporation Immunoassay using ascitic fluid
US4882423A (en) * 1984-10-02 1989-11-21 Calpis Food Industry Substance-conjugated complement component C1q
US5035995A (en) * 1984-10-02 1991-07-30 Calpis Food Industry Co., Ltd. Test method involving substance-conjugated complement component C1q
JPH03233358A (ja) * 1990-02-08 1991-10-17 Internatl Reagents Corp 抗原または抗体の高感度測定法
WO1996021675A2 (fr) * 1995-01-13 1996-07-18 Innogenetics N.V. Capture de micro-organismes a l'aide de constituants du complement

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 016, no. 015 (P - 1298) 14 January 1992 (1992-01-14) *

Also Published As

Publication number Publication date
AT409801B (de) 2002-11-25
WO2001092888A2 (fr) 2001-12-06
ATA9612000A (de) 2002-03-15
AU8382501A (en) 2001-12-11

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