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WO2001090315A3 - Production de neurones a partir de cellules souches - Google Patents

Production de neurones a partir de cellules souches Download PDF

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Publication number
WO2001090315A3
WO2001090315A3 PCT/CA2001/000756 CA0100756W WO0190315A3 WO 2001090315 A3 WO2001090315 A3 WO 2001090315A3 CA 0100756 W CA0100756 W CA 0100756W WO 0190315 A3 WO0190315 A3 WO 0190315A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
medium
neurons
production
heat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2001/000756
Other languages
English (en)
Other versions
WO2001090315A2 (fr
Inventor
Harold A Robertson
Cindee Leopold
Victor Rafuse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NOVANEURON Inc
Original Assignee
NOVANEURON Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NOVANEURON Inc filed Critical NOVANEURON Inc
Priority to AU2001261984A priority Critical patent/AU2001261984A1/en
Publication of WO2001090315A2 publication Critical patent/WO2001090315A2/fr
Publication of WO2001090315A3 publication Critical patent/WO2001090315A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Neurosurgery (AREA)
  • Cardiology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de production de neurones consistant à incuber des cellules souches dans un milieu de croissance contenant un facteur de croissance, à séparer le milieu de croissance des cellules souches, à traiter thermiquement le milieu de croissance afin de produire un milieu traité thermiquement, puis à incuber ces cellules souches dans un milieu traité comprenant le milieu traité thermiquement. Elle concerne aussi des neurones et le milieu conditionné produits au moyen de ce procédé ainsi que leurs utilisations.
PCT/CA2001/000756 2000-05-25 2001-05-25 Production de neurones a partir de cellules souches Ceased WO2001090315A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001261984A AU2001261984A1 (en) 2000-05-25 2001-05-25 Production of neurons from stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20680700P 2000-05-25 2000-05-25
US60/206,807 2000-05-25

Publications (2)

Publication Number Publication Date
WO2001090315A2 WO2001090315A2 (fr) 2001-11-29
WO2001090315A3 true WO2001090315A3 (fr) 2002-08-01

Family

ID=22768053

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000756 Ceased WO2001090315A2 (fr) 2000-05-25 2001-05-25 Production de neurones a partir de cellules souches

Country Status (2)

Country Link
AU (1) AU2001261984A1 (fr)
WO (1) WO2001090315A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62107796A (ja) * 1985-11-07 1987-05-19 Morinaga Milk Ind Co Ltd 新規なヒト顆粒球−単球系幹細胞増殖因子の製造法
JPH07265068A (ja) * 1994-03-31 1995-10-17 Snow Brand Milk Prod Co Ltd 細胞培養に用いる培地用添加剤
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62107796A (ja) * 1985-11-07 1987-05-19 Morinaga Milk Ind Co Ltd 新規なヒト顆粒球−単球系幹細胞増殖因子の製造法
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
JPH07265068A (ja) * 1994-03-31 1995-10-17 Snow Brand Milk Prod Co Ltd 細胞培養に用いる培地用添加剤

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DAADI MARCEL M ET AL: "Generation of tyrosine hydroxylase-producing neurons from precursors of the embryonic and adult forebrain.", JOURNAL OF NEUROSCIENCE, vol. 19, no. 11, 1 June 1999 (1999-06-01), pages 4484 - 4497, XP002191901, ISSN: 0270-6474 *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; XP002191904 *
DATABASE WPI Section Ch Week 198725, Derwent World Patents Index; Class B04, AN 1987-175153, XP002191905 *
DATABASE WPI Section Ch Week 199550, Derwent World Patents Index; Class B04, AN 1995-388680, XP002191906 *
FERNANDES K: "Recent advances in neural stem cell technologies review", NEURAL NOTES, vol. 19, 10 November 2001 (2001-11-10), XP002191903 *
KAWASAKI HIROSHI ET AL: "Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.", NEURON, vol. 28, no. 1, October 2000 (2000-10-01), pages 31 - 40, XP002191902, ISSN: 0896-6273 *
REYNOLDS B A ET AL: "CLONAL AND POPULATION ANALYSES DEMONSTRATE THAT AN EGF-RESPONSIVE MAMMALIAN EMBRYONIC CNS PRECURSOR IS A STEM CELL", DEVELOPMENTAL BIOLOGY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 175, 1996, pages 1 - 13, XP000916284, ISSN: 0012-1606 *
SHINGO T ET AL: "Robust induction of tyrosine hydroxylase in embryonic and adult striatal neural stem cell-derived neurons in defined media and the absence of gene transfer.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 26, no. 1-2, 2000, 30th Annual Meeting of the Society of Neuroscience;New Orleans, LA, USA; November 04-09, 2000, pages abstract - 312.18, XP002203033 *
WEISS S ET AL: "Is there a neural stem cell in the mammalian forebrain?", TRENDS IN NEUROSCIENCE, ELSEVIER, AMSTERDAM, NL, vol. 19, no. 9, 1996, pages 387 - 393, XP002098734, ISSN: 0166-2236 *

Also Published As

Publication number Publication date
WO2001090315A2 (fr) 2001-11-29
AU2001261984A1 (en) 2001-12-03

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