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WO2001088107A3 - Alpha-amylase variants with altered 1,6-activity - Google Patents

Alpha-amylase variants with altered 1,6-activity Download PDF

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Publication number
WO2001088107A3
WO2001088107A3 PCT/DK2001/000323 DK0100323W WO0188107A3 WO 2001088107 A3 WO2001088107 A3 WO 2001088107A3 DK 0100323 W DK0100323 W DK 0100323W WO 0188107 A3 WO0188107 A3 WO 0188107A3
Authority
WO
WIPO (PCT)
Prior art keywords
alpha
activity
altered
amylase variants
variants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2001/000323
Other languages
French (fr)
Other versions
WO2001088107A2 (en
Inventor
Allan Svendsen
Christel Thea Joergensen
Bjarne Roenfeldt Nielsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to AU2001258229A priority Critical patent/AU2001258229A1/en
Publication of WO2001088107A2 publication Critical patent/WO2001088107A2/en
Publication of WO2001088107A3 publication Critical patent/WO2001088107A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

The present invention relates to variants of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in the alpha-1,6-D-glucosidic branch linkage cleavage activity of amylopectin and limit dextrins.
PCT/DK2001/000323 2000-05-12 2001-05-10 Alpha-amylase variants with altered 1,6-activity Ceased WO2001088107A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001258229A AU2001258229A1 (en) 2000-05-12 2001-05-10 Alpha-amylase variants with altered 1,6-activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200000779 2000-05-12
DKPA200000779 2000-05-12

Publications (2)

Publication Number Publication Date
WO2001088107A2 WO2001088107A2 (en) 2001-11-22
WO2001088107A3 true WO2001088107A3 (en) 2002-06-27

Family

ID=8159490

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2001/000323 Ceased WO2001088107A2 (en) 2000-05-12 2001-05-10 Alpha-amylase variants with altered 1,6-activity

Country Status (2)

Country Link
AU (1) AU2001258229A1 (en)
WO (1) WO2001088107A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8852912B2 (en) 2009-04-01 2014-10-07 Danisco Us Inc. Compositions and methods comprising alpha-amylase variants with altered properties

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EP4269684A3 (en) 2004-07-05 2024-02-21 Novozymes A/S Alpha-amylase variants with altered properties
US20060147581A1 (en) 2004-12-22 2006-07-06 Novozymes A/S Hybrid enzymes
EP1926753B1 (en) * 2004-12-22 2010-11-24 Novozymes A/S Hybrid enzymes consisting of an endo-amylase first amino acid sequence and a carbohydrate-binding module as second amino acid sequence
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AU2008325250B2 (en) 2007-11-05 2013-06-13 Danisco Us Inc. Variants of Bacillus sp. TS-23 alpha-amylase with altered properties
EP2534236B1 (en) 2010-02-10 2018-05-30 Novozymes A/S Variants and compositions comprising variants with high stability in presence of a chelating agent
US20160108387A1 (en) 2013-05-29 2016-04-21 Danisco Us Inc. Novel metalloproteases
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EP3241890B1 (en) * 2016-05-03 2019-06-26 The Procter and Gamble Company Automatic dishwashing detergent composition
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US8852912B2 (en) 2009-04-01 2014-10-07 Danisco Us Inc. Compositions and methods comprising alpha-amylase variants with altered properties

Also Published As

Publication number Publication date
AU2001258229A1 (en) 2001-11-26
WO2001088107A2 (en) 2001-11-22

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