[go: up one dir, main page]

WO2001088183A3 - Method for the isolation of helicobacter pylori - Google Patents

Method for the isolation of helicobacter pylori Download PDF

Info

Publication number
WO2001088183A3
WO2001088183A3 PCT/US2001/040756 US0140756W WO0188183A3 WO 2001088183 A3 WO2001088183 A3 WO 2001088183A3 US 0140756 W US0140756 W US 0140756W WO 0188183 A3 WO0188183 A3 WO 0188183A3
Authority
WO
WIPO (PCT)
Prior art keywords
isolation
pylori
helicobacter pylori
kits
isolating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/040756
Other languages
French (fr)
Other versions
WO2001088183A2 (en
Inventor
Qunsheng Song
Gerald W Zirnstein
Benjamin D Gold
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centers of Disease Control and Prevention CDC
US Department of Health and Human Services
Original Assignee
Centers of Disease Control and Prevention CDC
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centers of Disease Control and Prevention CDC, US Department of Health and Human Services filed Critical Centers of Disease Control and Prevention CDC
Priority to AU2001259869A priority Critical patent/AU2001259869A1/en
Publication of WO2001088183A2 publication Critical patent/WO2001088183A2/en
Publication of WO2001088183A3 publication Critical patent/WO2001088183A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and kits are disclosed for isolating urease-positive bacteria by exposing a sample for 1 to 60 minutes to a media containing urea along with simultaneous or subsequent exposure to pH below 3.0. In one embodiment, the bacteria is H. pylori and the acidic conditions are provided by addition of HC1. These methods and kits are especially useful for isolating or detecting H. pylori in samples, such as saliva samples, contaminated by other microorganisms.
PCT/US2001/040756 2000-05-18 2001-05-16 Method for the isolation of helicobacter pylori Ceased WO2001088183A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001259869A AU2001259869A1 (en) 2000-05-18 2001-05-16 Method for the isolation of helicobacter pylori

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20532000P 2000-05-18 2000-05-18
US60/205,320 2000-05-18

Publications (2)

Publication Number Publication Date
WO2001088183A2 WO2001088183A2 (en) 2001-11-22
WO2001088183A3 true WO2001088183A3 (en) 2002-10-03

Family

ID=22761721

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/040756 Ceased WO2001088183A2 (en) 2000-05-18 2001-05-16 Method for the isolation of helicobacter pylori

Country Status (2)

Country Link
AU (1) AU2001259869A1 (en)
WO (1) WO2001088183A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9328326B2 (en) 2006-08-10 2016-05-03 Merck Patent Gmbh Method for isolating cells
CN113201457B (en) * 2021-04-22 2023-05-09 成都欧林生物科技股份有限公司 Pretreatment method for helicobacter pylori culture detection

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4923801A (en) * 1987-04-13 1990-05-08 The University Of Virginia Alumni Patents Foundation Compositions and methods for the enrichment and isolation of Campylobacter pylori and related organisms from biological specimens and the environment

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4923801A (en) * 1987-04-13 1990-05-08 The University Of Virginia Alumni Patents Foundation Compositions and methods for the enrichment and isolation of Campylobacter pylori and related organisms from biological specimens and the environment

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MARSHALL B J ET AL: "UREA PROTECTS HELICOBACTER-PYLORI FROM THE BACTERICIDAL EFFECT OF ACID", GASTROENTEROLOGY, vol. 99, no. 3, 1990, pages 697 - 702, XP008004906, ISSN: 0016-5085 *
SONG QUNSHENG ET AL: "A novel method for isolation of Helicobacter pylori from contaminated human and environmental specimens.", GASTROENTEROLOGY, vol. 120, no. 5 Supplement 1, April 2001 (2001-04-01), 102nd Annual Meeting of the American Gastroenterological Association and Digestive Disease Week;Atlanta, Georgia, USA; May 20-23, 2001, April, 2001, pages A.580, XP008005234, ISSN: 0016-5085 *
SONG QUNSHENG ET AL: "Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 39, no. 5, May 2001 (2001-05-01), pages 1967 - 1968, XP002202780, ISSN: 0095-1137 *
STEVENSON TIMOTHY H ET AL: "Development of a selective medium for isolation of Helicobacter pylori from cattle and beef samples.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 66, no. 2, February 2000 (2000-02-01), pages 723 - 727, XP002202779, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
WO2001088183A2 (en) 2001-11-22
AU2001259869A1 (en) 2001-11-26

Similar Documents

Publication Publication Date Title
WO2000073794A3 (en) Method for separating cells using immunorosettes
CA2126250A1 (en) Method, reagents and kits for the detection of neisseria gonorrhoeae
EP1383914A4 (en) Improved method for detection of atp
IL147038A0 (en) Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
EP1236043A4 (en) ELECTROCHEMICAL METHOD FOR ENRICHING A MICROORGANISM AND A BIOSENSOR FOR ANALYZING ORGANIC SUBSTANCES AND THE BOD
WO2003063693A3 (en) Method for detecting microorganisms
WO2001057247A3 (en) Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
CA2354379A1 (en) Method for isolating dna from biological materials
AU2323399A (en) Method and compositions for detecting luciferase in biological samples
DE60129594D1 (en) DEVICE FOR CLEANING WATER CONTAINING SOLVED ORGANIC SUBSTANCES AND TRACKS OF HARMFUL SUBSTANCES
AU2747597A (en) Method, compositions and kit for detection and identification of microorganisms
AU2127888A (en) Detection methods
EP1310793A4 (en) Substrate activation kit and method of detecting dna or the like by using the same
CA2339821A1 (en) Enzymatic measurement of mycophenolic acid
WO2005028680A3 (en) Methods, compositions, and kits for the concentration and detection of microorganisms
WO2001088183A3 (en) Method for the isolation of helicobacter pylori
WO2002010744A1 (en) Method of pretreating sample
WO2002027316A3 (en) Methods and kits for decreasing interferences of assays samples containing plasma or serum in specific binding assays by using a large polycation
WO2001054817A3 (en) Cuvettes, automated biological analysis apparatus using such cuvettes, means for transferring said cuvettes in such an apparatus and transfer method
AU2001287704A1 (en) Method and agent for determining total water hardness
ATE414786T1 (en) METHOD FOR DETECTING A WHOLE HOMOCYSTEINE
WO2001068900A3 (en) Method for specifically detecting microorganisms by polymerase chain reaction
WO1999005159A3 (en) Staphylococcus specific nucleic acid molecule, test kit and utilization of the nucleic acid molecule and test kit
WO2004034012A3 (en) Coded particles for multiplexed analysis of biological samples
AU6415394A (en) Test kits and methods for rapidly testing for contamination by microorganisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP