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WO2001088179A1 - Procede permettant de realiser un frottis cellulaire de maniere automatique pour des examens cytologiques - Google Patents

Procede permettant de realiser un frottis cellulaire de maniere automatique pour des examens cytologiques Download PDF

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Publication number
WO2001088179A1
WO2001088179A1 PCT/KR2001/000807 KR0100807W WO0188179A1 WO 2001088179 A1 WO2001088179 A1 WO 2001088179A1 KR 0100807 W KR0100807 W KR 0100807W WO 0188179 A1 WO0188179 A1 WO 0188179A1
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WO
WIPO (PCT)
Prior art keywords
cells
slides
fixation
staining
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2001/000807
Other languages
English (en)
Inventor
Won-Bae Lee
Hae-Won Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU2001258887A priority Critical patent/AU2001258887A1/en
Publication of WO2001088179A1 publication Critical patent/WO2001088179A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on

Definitions

  • the present invention relates generally to methods for the use in the pathological test of cancer such as cervical cancer. More particularly, the present invention provides the methods for automatic slide preparation by spreading sample cells evenly on the slides, which is critical for the accurate diagnosis.
  • the traditional methods of spreading test specimens on the slide (300) for the cancer diagnosis test were proceeded as described in the figure 1. In the traditional methods, sampling was done by collecting the cells from the patient's cervix with cotten swabs or brushes. After cells have been collected, they were spreaded onto the slides. Then, the slides(300) were inserted into the fixation solution in the fixation container (100) to fix the cells(l ⁇ ) and sent to pathology laboratory.
  • Clinical pathologist took out the sample slides, dried and labeled the slides with the name and the registration number of the patients. Many labeled slides(300) placed in the staining racks (40) were inserted into the staining solution, were taken out and covered with cover glass(500) for the observation of the stained cells. The slides prepared as described above were examined in the pathology laboratory.
  • the disadvantage of the prior art process is in overlapping of sample cells due to uneven distribution of cells over the slides(300) preventing the accurate microscopic observation and causing the erroneous diagnosis of the cancer.
  • Other disadvantages in former processes are the inconvenience of collecting and transferring of the cells into the slides, the high misdiagnosis rate due to uneven distribution of cells in the slides and the laborous work done by clinical pathologist, such as drying the slides, labeling and staining slides after placing in the staining rack.
  • the procedure utilized for spreading cells in many of domestic hospital costs a lot and requires the imported test equipments, since it is done exactly same as in foreign countries. The process is not automated so that it can not save the time and effort.
  • the present invention provides an improved method of spreading test sample cells on the slides for cancer diagnostic examination comprising the steps of putting the collected cervical cells into funnel-type fixation container for the fixation (Fixation step make the cells maintain their morphology, even the cells are dead.), putting the fixation container containing the cells on the separated machinery system followed by sucking the cells precipitated in the bottom of fixation container with the sucking tubes attached in the machinery system, and spreading the sucked cells onto slides fed automatically.
  • the advantage of the present invention is in improving uniform spreading of thicker cells in the slides compared to former processes without overlapped layers of cells, which contributes to lower the erroneous diagnosis rate, and automating the slide preparation process, which save laborous work done by clinical pathologist, such as drying the slides out of fixation container, labeling and staining them after placing in the staining rack. Additionally, This invention is advantageous for replacing the imported equipments with domestic goods.
  • FIG. 1 is the preferred embodiment of the prior art including the procedure spreading cells on the slides for the cancer diagnostic test.
  • FIG. 2 is the preferred embodiment of present invention including the procedure spreading sample cells on the slides utilizing the fixation container of this invention.
  • FIG. 3 is a cross-sectional view of the fixation container of the present invention.
  • fixation container 2 sucking tube 3: slide 4: rack 5:cover glass
  • the preferred embodiments of this invention comprises the steps of putting the collected cervical cells(l ⁇ ) into funnel-type fixation container(l) for the fixation, the steps of sucking the cells(l ⁇ ) precipitated in the bottom of fixation container (1) with the sucking tubes(2) and spreading the sucked cells onto slides(3), the steps of placing many slides(3) spreaded with cells in the staining racks (4), the steps of inserting racks (4) with slides into the staining solution for staining, the steps of covering the cells on the slides with cover slides for protection, and the step of examining the stained cells under the microscope.
  • Another preferred embodiment of this invention comprises the steps of putting the collected cervical cells(l ⁇ ) into funnel-type fixation container(l) for the fixation, the steps of collecting the cells(l ⁇ ) in the spraying container and spraying the sucked cells onto slides(3) for the even distribution of the cells, the steps of inserting the slides (4) into the rack for staining in an automatic way, drying and examining the stained cells.
  • test cells collected from the patient's cervix with cotten swabs or brushes, inserted and stored for a few minutes on the fixation solution in the funnel-type container (1) maintain their morphology even in the dead stage and concentrated at the bottom of the container, while the blood floated to the top of the solution with alcohols.
  • the cells already fixed at the time of spreading, is strong enough not to be damaged or aggregated, which is good for better examination.
  • the slides spreaded with 0.02-0.04ml volume cells were labeled with patient's name, placed in the staining racks (4), inserted into the staining solution for staining, labeled with patient's name, covered with cover slides for protection, and examined under the microscope for cancer diagnosis.
  • Another preferred embodiments of this invention comprise the steps of putting the collected cervical cells(l ⁇ ) into funnel-type fixation container(l) for the fixation, the steps of collecting the cells(l ⁇ ) in the spraying container and spraying the sucked cells onto slides(3) for the even distribution of the cells without overlapping, which enable the pathologist to examine more cells selectively under the microscope for accurate diagnosis.
  • the processes of this invention carried out as described above enable examiner to draw a lot of cells by storing the cells taken from patients's cervix and collecting in the bottom of funnel-type fixation container. This is significant improvements enabling uniform spreading of thicker cells in the slides compared to former processes, without overlapped layers of cells, which contributes to lower the erroneous diagnosis rate and save the number of workers needed for staining and spreading, process. Additionally, This invention is advantageous for replacing the imported equipments with domestic goods, which leads to save the foreign currency and to improve quality of domestic test equipments

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé pour étaler des cellules d'échantillon d'essai sur des lames afin d'effectuer un dépistage du cancer. Ce procédé consiste à placer les cellules cervicales recueillies dans un contenant de fixation en entonnoir ; à placer le contenant, à l'intérieur duquel se trouvent les cellules, sur un système mécanique séparé, puis à aspirer les cellules précipitées au fond du contenant au moyen de tubes d'aspiration fixés au système mécanique, et à étaler les cellules aspirées sur des lames pour assurer une répartition homogène. L'un des avantages de l'invention réside dans le fait que la répartition des cellules épaisses sur les lames est plus homogène que dans le cas des procédés antérieurs. Il ne se produit pas de superposition des couches de cellules, ce qui contribue à faire baisser les taux de diagnostics erronés. Un autre avantage réside dans l'automatisation du procédé de préparation des lames qui évite au médecin biologiste d'effectuer des tâches laborieuses telles que le séchage des lames hors du contenant de fixation, le marquage et la coloration des lames après les avoir placées dans le support de coloration. De plus, ce procédé permet de remplacer les équipements importés par des produits d'origine nationale et donc d'économiser des devises étrangères et de réduire le coût des traitements médicaux.
PCT/KR2001/000807 2000-05-19 2001-05-17 Procede permettant de realiser un frottis cellulaire de maniere automatique pour des examens cytologiques Ceased WO2001088179A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001258887A AU2001258887A1 (en) 2000-05-19 2001-05-17 Auto cell-smearing method for cytology

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR2000/27131 2000-05-19
KR1020000027131A KR20010105975A (ko) 2000-05-19 2000-05-19 검사용 세포 자동 도말 처리 방법

Publications (1)

Publication Number Publication Date
WO2001088179A1 true WO2001088179A1 (fr) 2001-11-22

Family

ID=19669350

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2001/000807 Ceased WO2001088179A1 (fr) 2000-05-19 2001-05-17 Procede permettant de realiser un frottis cellulaire de maniere automatique pour des examens cytologiques

Country Status (3)

Country Link
KR (1) KR20010105975A (fr)
AU (1) AU2001258887A1 (fr)
WO (1) WO2001088179A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018513984A (ja) * 2015-04-06 2018-05-31 ナノサイトミクス，エルエルシー 自動化標本堆積システム及び関連する方法
CN108827731A (zh) * 2018-06-16 2018-11-16 宋金鸿 妇科白带样本检查辅助装置

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160099283A (ko) 2015-02-12 2016-08-22 (주)로봇앤드디자인 솔루션용기
KR101669237B1 (ko) 2015-02-12 2016-11-09 (주)로봇앤드디자인 검사용 세포 자동 도말장치

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4059404A (en) * 1975-03-29 1977-11-22 Battelle-Institute E.V. Swab and method of taking cell smears for diagnostic examination
JPS5988084A (ja) * 1982-11-15 1984-05-21 Toshiba Corp 自動染色装置
JPS59188541A (ja) * 1983-04-11 1984-10-25 Yoko Morishita 細胞診検査における細胞の剥落防止方法および剥落防止用スプレ−
US5191899A (en) * 1991-12-12 1993-03-09 Baal Associates, Inc. Pap smear collection device with bristles oriented in a plane

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54100787U (fr) * 1977-12-27 1979-07-16
US4378333A (en) * 1980-12-11 1983-03-29 Laipply Thomas C Device for preparing blood smears on glass slides and method therefor
IL74774A (en) * 1984-04-23 1988-05-31 Abbott Lab Method for the preparation of immunocytochemical slides with a polylysine solution
JPH0743373B2 (ja) * 1989-06-27 1995-05-15 アイエスケー医学書院器械株式会社 喀痰集細胞検診方法
US5494828A (en) * 1994-07-13 1996-02-27 Leopando; Mark E. Slide dispensing device and method
JP3976356B2 (ja) * 1995-03-31 2007-09-19 シスメックス株式会社 自動標本作製装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4059404A (en) * 1975-03-29 1977-11-22 Battelle-Institute E.V. Swab and method of taking cell smears for diagnostic examination
JPS5988084A (ja) * 1982-11-15 1984-05-21 Toshiba Corp 自動染色装置
JPS59188541A (ja) * 1983-04-11 1984-10-25 Yoko Morishita 細胞診検査における細胞の剥落防止方法および剥落防止用スプレ−
US5191899A (en) * 1991-12-12 1993-03-09 Baal Associates, Inc. Pap smear collection device with bristles oriented in a plane

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018513984A (ja) * 2015-04-06 2018-05-31 ナノサイトミクス，エルエルシー 自動化標本堆積システム及び関連する方法
EP3280543A4 (fr) * 2015-04-06 2018-12-26 Nanocytomics LLC Systèmes de dépôt d'échantillon automatisé et procédés associés
JP2021181992A (ja) * 2015-04-06 2021-11-25 ナノサイトミクス，エルエルシー 自動化標本堆積システム及び関連する方法
US11573242B2 (en) 2015-04-06 2023-02-07 Nanocytomics, LLC Automated specimen deposition systems and associated methods
US12158475B2 (en) 2015-04-06 2024-12-03 Nanocytomics, LLC Automated specimen deposition systems and associated methods
CN108827731A (zh) * 2018-06-16 2018-11-16 宋金鸿 妇科白带样本检查辅助装置
CN108827731B (zh) * 2018-06-16 2020-10-13 马玉玲 妇科白带样本检查辅助装置

Also Published As

Publication number Publication date
KR20010105975A (ko) 2001-11-29
AU2001258887A1 (en) 2001-11-26

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