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WO2001084146B1 - Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr - Google Patents

Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr

Info

Publication number
WO2001084146B1
WO2001084146B1 PCT/IB2001/000957 IB0100957W WO0184146B1 WO 2001084146 B1 WO2001084146 B1 WO 2001084146B1 IB 0100957 W IB0100957 W IB 0100957W WO 0184146 B1 WO0184146 B1 WO 0184146B1
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
spectrometry
immuno
pcr
sensitive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2001/000957
Other languages
English (en)
Other versions
WO2001084146A2 (fr
WO2001084146A3 (fr
Inventor
Curt T Reimann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Forskarpatent I SYD AB
Original Assignee
Forskarpatent I SYD AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forskarpatent I SYD AB filed Critical Forskarpatent I SYD AB
Priority to AU2001258700A priority Critical patent/AU2001258700A1/en
Publication of WO2001084146A2 publication Critical patent/WO2001084146A2/fr
Publication of WO2001084146A3 publication Critical patent/WO2001084146A3/fr
Publication of WO2001084146B1 publication Critical patent/WO2001084146B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

La présente invention concerne un procédé de détection de substances, selon lequel on procède à la détection immunologique d'une substance, on amplifie ensuite un acide nucléique marqueur lié à un anticorps, et on identifie le marqueur amplifié. Le procédé de détection de l'invention fait appel à la spectrométrie de masse qui, en décelant la présence et en identifiant la masse de l'acide nucléique marqueur, permet de détecter la liaison spécifique de l'anticorps avec un antigène.
PCT/IB2001/000957 2000-05-04 2001-05-04 Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr Ceased WO2001084146A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001258700A AU2001258700A1 (en) 2000-05-04 2001-05-04 A sensitive, multiplexed mass-spectrometry-based biosensor based on immuno-pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0001670A SE0001670D0 (sv) 2000-05-04 2000-05-04 Mass-spectrometry-based biosensor
SE0001670-9 2000-05-04

Publications (3)

Publication Number Publication Date
WO2001084146A2 WO2001084146A2 (fr) 2001-11-08
WO2001084146A3 WO2001084146A3 (fr) 2002-04-11
WO2001084146B1 true WO2001084146B1 (fr) 2002-07-04

Family

ID=20279569

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/000957 Ceased WO2001084146A2 (fr) 2000-05-04 2001-05-04 Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr

Country Status (3)

Country Link
AU (1) AU2001258700A1 (fr)
SE (1) SE0001670D0 (fr)
WO (1) WO2001084146A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
WO2001078652A2 (fr) 2000-04-14 2001-10-25 Metabolon, Inc. Procedes permettant de decouvrir des medicaments, de traiter des maladies et de poser des diagnostics a l'aide de la metabolomique
DE10200374A1 (de) * 2002-01-08 2003-07-24 Friz Biochem Gmbh Verfahren zur qualitativ und quantitativ vergleichenden Detektion chemischer Substanzen
CN101410530B (zh) * 2003-04-18 2013-03-27 贝克顿·迪金森公司 免疫-扩增
EP1753873A4 (fr) 2004-03-05 2008-11-05 Ohio Med College Procedes et compositions pour l'evaluation d'acides nucleiques et d'alleles
WO2005106036A2 (fr) * 2004-04-12 2005-11-10 Medical College Of Ohio Methodes et compositions pour analyser des analytes
EP1885890A4 (fr) * 2005-05-26 2010-01-20 Univ Boston Quantification d'acides nucléiques et de protéines au moyen d'étiquettes de masse d'oligonucléotides
WO2008033575A2 (fr) 2006-09-15 2008-03-20 Metabolon, Inc. Procédés d'identification de cheminements biochimiques
JP2009219388A (ja) * 2008-03-14 2009-10-01 Jsr Corp イムノｐｃｒ用磁性粒子、標的物質の検出方法、および標的物質の検出キット
AU2014248759B2 (en) 2013-03-13 2020-02-27 Meso Scale Technologies, Llc. Improved assay methods
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
EP3143401A4 (fr) 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Méthodes de dosage améliorées
AU2017370751B2 (en) 2016-12-09 2023-11-09 Ultivue, Inc. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
WO2018183876A1 (fr) 2017-03-31 2018-10-04 Ultivue, Inc. Échange adn-antigène et amplification
CN112189139B (zh) * 2018-09-26 2024-10-29 北卡罗来纳-查佩尔山大学 用于改进测定的化合物、组合物和方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996029432A1 (fr) * 1995-03-22 1996-09-26 Trustees Of Boston University Procedes de detection de paracoccidioides
DE19802576B4 (de) * 1998-01-23 2004-10-28 Xerion Pharmaceuticals Ag Verfahren zur gleichzeitigen Identifizierung von Proteinen und ihren Bindungspartnern
JP2003536048A (ja) * 1999-03-23 2003-12-02 バイオヴェイション リミテッド タンパク質の単離および分析

Also Published As

Publication number Publication date
AU2001258700A1 (en) 2001-11-12
WO2001084146A2 (fr) 2001-11-08
WO2001084146A3 (fr) 2002-04-11
SE0001670D0 (sv) 2000-05-04

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