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WO2001083437A1 - Procede d'extraction de pigment astaxanthine a partir de levure et son pigment extrait - Google Patents

Procede d'extraction de pigment astaxanthine a partir de levure et son pigment extrait Download PDF

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Publication number
WO2001083437A1
WO2001083437A1 PCT/KR2001/000719 KR0100719W WO0183437A1 WO 2001083437 A1 WO2001083437 A1 WO 2001083437A1 KR 0100719 W KR0100719 W KR 0100719W WO 0183437 A1 WO0183437 A1 WO 0183437A1
Authority
WO
WIPO (PCT)
Prior art keywords
pigment
yeast cells
astaxanthin
microwave
phaffia rhodozyma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2001/000719
Other languages
English (en)
Inventor
Ji Young Han
Seung Jae Lee
Myung Kyo Jung
Seok Keun Choi
Jung Seop Roh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Haitai Food Products Co Ltd
Haitai Confectionery Co Ltd
Original Assignee
Haitai Food Products Co Ltd
Haitai Confectionery Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU56807/01A priority Critical patent/AU5680701A/en
Application filed by Haitai Food Products Co Ltd, Haitai Confectionery Co Ltd filed Critical Haitai Food Products Co Ltd
Priority to JP2001580866A priority patent/JP2003531888A/ja
Priority to EP01930264A priority patent/EP1278725A1/fr
Publication of WO2001083437A1 publication Critical patent/WO2001083437A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
    • C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources

Definitions

  • the present invention relates to a process for extracting astaxanthin pigment from the yeast cells of Phaffia rhodozyma comprising the steps of i ) cultivating the yeast cells, ii ) treating yeast culture suspension with microwave to destroy the cell walls and microbodies, in) drying it or extracting astaxanthin pigment using the solvent selected from the group consisting of ethanol, methanol, acetone and mixture of them. Further, the present invention also relates to the astaxanthin extracted by above method.
  • the present invention relates to a process for extracting astaxanthin pigment from the yeast culture suspension further comprising i ) the microwave treatment step, in which the culture suspension is passed through the Teflon tube or is laid on the Teflon extraction vessel for irradiation of microwave 50—1000 watts at the frequency of 900 — 930 or 2400— 2500MHz ; ii ) the pigment separation step, in which the obtained pigment is concentrated at reduced pressure using rotary vacuum evaporator.
  • the microwave treatment also provides the sterility of other microorganisms as well as the destruction of cell walls and microbodies in yeast.
  • Astaxanthin (3,3'-dihydroxy- ⁇ , ⁇ '- carotene-4,4'-dione) is generally obtained from the yeast cell of Phaffia rhodozyma (Phytochemistry, 15, 1009, (1976)), blue-green algae of Haematococcus species (Phytochemistry, 20, 2561, (1981)) and Brev ⁇ bacterium (J. general and applied microbiology, 15, 127, (1969)). Further, it is chiefly distributed in the sea water animals and fresh water animals (Phytochemistry 15. 1003-1007 (1976)).
  • the astaxanthin pigment has an excellent anti-oxidation activity compared to other carotenoid pigment or tocopherol (Fisheries Sci., 62. 134-140 (1996)). It has been regarded that the astaxanthin pigment has an importance of medical use as well as that of edible pigment (Crit. Rev. Biotechnol. 11. 297-326 (1991)).
  • Phaffia rhodozyma have been developed.
  • a biochemical method comprising the step destroying cell walls using digestion enzyme, such as cellulase, hemicellulase and pectinase has been disclosed in Appl. and Environ. Microbiol. 35(6). 1155-1159(1978).
  • digestion enzyme such as cellulase, hemicellulase and pectinase
  • any of above disclosed methods can not afford ' the commercially available extracting method for astaxanthin, due to the destruction of astaxanthin pigment during the process and the low yield of astaxanthin.
  • the direct extraction method of astaxanthin using the solvent such as ethanol or acetone, also cannot be commercialized due to its high cost and low yield.
  • the inventors developed a microwave treatment method to destroy cell walls for efficient extraction of pigment and also designed the vessel and the tube for microwave irradiation. Therefore, the inventors accomplished effective and cost efficient method for obtaining astaxanthin pigment.
  • the object of the present invention is to provide a process for extracting astaxanthin pigment from the yeast cells of Phaffia rhodozyma comprising the steps of i ) cultivating yeast cells, ii ) suspending cultivated yeast cells with water i ⁇ ) treating culture suspension with microwave to destroy the cell walls and microbodies, and iy) drying obtained material containing astaxanthin pigment.
  • the another object of the present invention is to provide a process for extracting astaxanthin pigment from the yeast cells of Phaffia rhodozyma comprising the steps of i ) cultivating yeast cells, ii) suspending cultivated yeast cells with water i ⁇ ) treating culture suspension with microwave to destroy the cell walls and microbodies, and iv) extracting astaxanthin pigment using the solvent selected from the group consisting of ethanol, methanol, acetone and mixture of them.
  • the microwave treatment is selected from the continuous process in which the culture suspension is passed through Teflon tube, and the fixed process in which the culture suspension is laid on the Teflon extraction vessel, for irradiation of microwave 50 ⁇ 1000 watts at the frequency of 900 -930 or 2400 -2500MHz.
  • the present invention also provide a process for extracting astaxanthin pigment from the yeast cells of Phaffia rhodozyma, further comprising the pigment separation step, in which the obtained pigment is concentrated at reduced pressure using rotary vacuum evaporator.
  • the yield of astaxanthin pigment is 10 — 95 wt% from total carotenoid contained in the yeast cells of Phaffia rhodozyma, and the purity of astaxanthin pigment is 50—95 wt% of obtained material.
  • the amount of extraction solvent is 5 — 20 vol. part compared to 1 vol. part of suspension, or 5 —10 (vol./wt) part compared to 1 wt part of dried content of the yeast cells of Phaffia rhodozyma.
  • the further object of the present invention is to provide a method for using astaxanthin pigment as cosmetics, animal feeds or food additives.
  • FIG 1 shows a schematic view of continuous microwave treatment system using the Teflon tube to the suspension according to the present invention.
  • FIG 2 shows a schematic view of fixed microwave treatment system using the Teflon extraction vessel to the suspension according to the present invention.
  • FIG 3 shows an optical microscope ( X 1,000) photo of Phaffia rhodozyma without microwave treatment (A); Phaffia rhodozyma with microwave treatment (B) after cultivating it for 5 days according to the present invention.
  • FIG 4 shows HPLC data of obtained extract followed by i ) cultivation of Phaffia rhodozyma ii ) microwave treatment, and iii) ethanol extraction according to the present invention.
  • the microwaves When the microwaves are irradiated into the Phaffia rhodozyma according to the methods shown in FIG 1 or FIG 2, the free water and other dipoles in the cells are rotated according to the electric field alternation, which converts microwave energy into thermal energy. Then, the cell walls and microbodies (nucleus, mitochondria, Golgi apparatus) in the cells are destroyed according to the elevation of internal pressure followed by internal heating. Therefore, the microwave treatment enables the extraction of pigment without physical destruction of cell walls. Further, the extraction by organic solvent can be easily accomplished by the microwave treatment, because organic solvent can be easily diffused into the cells.
  • the inventors adopt a microwave treatment method and apply it to the extraction of astaxanthin pigment, which enables the destruction of hard and thick cell walls of Phaffia rhodozyma chiefly composed of ⁇ -glucan, especially, a -1,3-glucan.
  • the inventors design the microwave treatment systems for extracting astaxanthin effectively.
  • One microwave treatment system using the Teflon tube as shown in FIG 1 and another is fixed microwave treatment system using the Teflon extraction vessel as shown in FIG 2.
  • Teflon tube is inserted into the microwave oven and the length and diameter of Teflon tube are designed according to the microwave holding time and microwave treatment capacity.
  • the cultivated suspension is passed through the tube using Variable speed peristaltic pump (Model : AS-90361, Won Corporation, Korea).
  • Teflon extraction vessel is laid on the center of microwave oven and the amount of cultivated suspension is put on the vessel in microwave oven.
  • Teflon tube and pump are not required.
  • the output of microwave is in the range of 50—1,000 watts at the frequency of 916 or 2450MHz.
  • the microwaves are irradiated into Teflon extraction vessel or Teflon tube for 10—500 seconds.
  • Example 1 Pigment extraction yield test according to continuous or fixed microwave treatment system
  • the extraction yield of astaxanthin was measured under microwave illumination according to the conditions; microwave output 50-1,000 watts, at frequency of 916 or 2450MHz, for 10-500 seconds.
  • Cultivated yeast cells (concentration : lOg/L) were treated with microwave by i ) continuous microwave treatment system using the Teflon tube as shown in FIG 1 and ii ) fixed microwave treatment system using the Teflon extraction vessel as shown in FIG 2. 10 vol. part of ethanol was added to 1 vol. part of cultivated yeast suspension. After extraction at 30 °C for 24 hours, the extract was laid at -20 °C for more than 30 minutes to remove the lipid.
  • the astaxanthin extract was obtained by concentrating the supernatant at reduced pressure.
  • absorbance was measured adding acetone by UV/VIS Spectrophotometer Biochrom 4060, Pharmacia, German at 478nm.
  • Control group was prepared by following methods. 1ml of preheated (55 °C) stock solution of dimethylsulf oxide (DMSO) was added to the cultivated yeast cells. Then, the mixture was vigorously agitated in order to fully destroy the yeast cells. To extract the pigment, 1ml of acetone, 1ml of petroleum ether and 1ml of sodium chloride (20%) were added in this order. On storaging in the refrigerator, the solution was centrifuged at 10,000 X g for 5 minutes. After obtaining petroleum ether layer in which the pigment was dissolved, the petroleum ether was evaporated at reduced pressure. 1ml of acetone was added to obtained material and the absorbance was measured at 478nm for confirming the pigment extract.
  • DMSO dimethylsulf oxide
  • the pigment extraction yield was calculated by following equation.
  • A the absorbance of control group after destroying cell walls using DMSO at 478nm
  • B the absorbance of soluble matter by organic solvent in experimental group after irradiation of microwave at 478nm
  • the pigment destruction rate was calculated by following equation.
  • the pigment destruction rate ⁇ A-(B+C) ⁇ /A X l00
  • A the absorbance of control group after destroying cell walls using DMSO at 478nm
  • B the absorbance of soluble matter by organic solvent in experimental group after irradiation of microwave at 478nm
  • C the absorbance of insoluble matter by organic solvent in experimental group after irradiation of microwave at 478nm
  • Table 1 shows the result of pigment extraction yield and pigment destruction rate according to the variation of frequency, time and output. All results are mean values of three times experiments. Table 1.
  • Example 2 Pigment extraction yield test according to extraction solvent and time after microwave irradiation
  • Example 1 frequency 2450MHz; output 500 watts; and irradiation time 60 seconds were adjusted for microwave treatment.
  • the cultivation suspension has cell density of lOg/L.
  • the amount of extraction solvent was 10 vol. part compared to 1 vol. part of cultivated suspension.
  • the extraction temperature was at 30 °C .
  • the astaxanthin extract was obtained by using rotary vacuum evaporator followed by agitation in the dark room. After adding acetone, the absorbance of pigment extract was measured at 478nm. Finally, pigment extraction yield was measured using UV/VIS Spectrophotometer Biochrom 4060.
  • Table 2 shows the result of pigment extraction yield and pigment destruction rate according to the variation of solvent and time, results are mean values of three times experiments.
  • Control group shows the absorbance extracted by ethanol from the cultivated suspension without microwave treatment
  • Example 3 Pigment extraction yield test according to cell density of cell cultivation suspension at the time of microwave irradiataion
  • Example 2 The experiment was carried out as the same manner of Example 2 except that cell densities of cell cultivation suspensions were varied.
  • the cell density of cell cultivation suspension was evaluated by the dried weight of yeast cells per liter.
  • pigment extraction yield and pigment destruction rate were measured using UV/VIS Spectrophotometer Biochrom 4060.
  • Table 3 shows the result of pigment extraction yield and pigment destruction rate according to cell density of cell cultivation suspension at the time of microwave irradiataion. All results are mean values of three times experiments.
  • Example 4 Pigment extraction yield test according to extraction temperature and time after microwave irradiation
  • Example 2 The experiment was carried out as the same manner of Example 2 except that the extraction temperatures were varied. Finally, pigment extraction yield was measured using UV/VIS Spectrophotometer Biochrom 4060.
  • Table 4 shows the result of pigment extraction yield and pigment destruction rate according to the variation of extraction temperature and time. All results are mean values of three times experiments.
  • Example 2 The experiment was carried out as the same manner of Example 2 except that the extraction solvent volumes were varied. The extraction time was 24 hours and extraction temperature was 30 °C . Finally, pigment extraction yield was measured using UV/VIS Spectrophotometer Biochrom 4060.
  • Table 5 shows the result of pigment extraction yield and pigment destruction rate according to the variation of extraction solvent volume. All results are mean values of three times experiments.
  • the total carotenoid contents were measured according to known method. 1ml of cultivated suspension was centrifuged and the precipitate was washed with steriled water twice. Then, 1ml of preheated (55 °C) stock solution of dimethylsulf oxide (DMSO) was added. Then, the mixture was vigorously agitated in order to fully destroy the yeast cells.
  • DMSO dimethylsulf oxide
  • Example 5 Using 1ml of pigment extract solution in Example 5, the solvent was evaporated at reduced pressure. 1ml of petroleum ether was added and the absorbance was measured at 474nm. The extraction yield of pigment was measured by comparing the amount of pigment extracted by dimethylsulfoxide (100%).
  • Total carotenoid contents were measured by using following equation which contains 1% extinction coefficient (2,100) and dried content of cells (Appl. and Environ. Microbiol., 55. 116-124(1989)). From ethanol extract, the contents of astaxanthin pigment was measured after evaporating extraction solvent at vacuum and reduced pressure. Then, obtained astaxanthin pigment was dissolved in chloroform before analyzing HPLC (Waters 486, USA). The HPLC data was shown in FIG 4.
  • the cells were obtained after continuous flow centrifuges (7,000 X g). The obtained cells vijere washed twice using clean water and the content of water made to be less than 10%. Using spray dryers, vacuum drum dryers or trays dryers, the Phaffia rhodozyma dried product was manufactured.

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Abstract

La présente invention concerne un procédé d'extraction de pigments astaxanthine à partir de cellules de Phaffia rhodozyma comprenant les étapes consistant (i) à mettre en culture la levure, (ii) à traiter une suspension de culture de levure avec des micro-ondes afin de détruire les parois et les microcorps cellulaires, (iii) à la sécher ou à extraire le pigment astaxanthine à l'aide du solvant choisi dans le groupe comprenant l'éthanol, le méthanol, l'acétone et un mélange de ceux-ci. De plus, la présente invention concerne également l'astaxanthine extraite par le procédé précité.
PCT/KR2001/000719 2000-05-04 2001-05-02 Procede d'extraction de pigment astaxanthine a partir de levure et son pigment extrait Ceased WO2001083437A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU56807/01A AU5680701A (en) 2000-05-04 2001-04-30 Process for extracting astaxanthin pigment from yeast and extracted pigment thereof
JP2001580866A JP2003531888A (ja) 2000-05-04 2001-05-02 酵母からアスタキサンチン色素を抽出するためのプロセスおよびその抽出された色素
EP01930264A EP1278725A1 (fr) 2000-05-04 2001-05-02 Procede d'extraction de pigment astaxanthine a partir de levure et son pigment extrait

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KR2000/23851 2000-05-04
KR10-2000-0023851A KR100388110B1 (ko) 2000-05-04 2000-05-04 효모 균주에서 아스타산틴 색소 추출방법 및 추출된 색소

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WO (1) WO2001083437A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005075575A1 (fr) * 2004-02-10 2005-08-18 Nestec S.A. Compositions contenant des isomeres cis d'un compose carotenoide
WO2009028643A1 (fr) 2007-08-29 2009-03-05 Nippon Oil Corporation Procédé de production de caroténoïde
EP2090182A1 (fr) * 2008-01-31 2009-08-19 Yamaha Hatsudoki Kabushiki Kaisha Procédé d'amélioration du goût d'un extrait contenant de l'astaxanthine
US7851199B2 (en) 2005-03-18 2010-12-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2011145113A2 (fr) 2010-05-17 2011-11-24 Dynadis Biotech India Pvt Ltd Procédé de production de cristaux de bêta-carotène et de lycopène de haute pureté à partir de biomasse fongique
US8097761B2 (en) 2006-03-28 2012-01-17 Jx Nippon Oil & Energy Corporation Process for production of carotenoid
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
EP2402455A4 (fr) * 2009-02-27 2014-10-08 Jx Nippon Oil & Energy Corp Procédé de production d'un caroténoïde
EP2728012A4 (fr) * 2011-06-30 2015-02-25 Kaneka Corp Procédé de production d'une composition de caroténoïdes

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100411364B1 (ko) * 2000-08-04 2003-12-18 해태제과식품주식회사 녹조류에서 아스타산틴 색소 추출방법 및 추출된 색소
KR20010044621A (ko) * 2001-03-12 2001-06-05 박인배 아스타산틴을 함유한 식품 또는 건강보조식품
KR100465555B1 (ko) * 2002-05-24 2005-01-13 이은규 아스타산틴의 제조방법
CN116508996B (zh) * 2023-05-23 2025-02-11 华南理工大学 一种类胡萝卜素乳液及其制备方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4156090A (en) * 1975-11-30 1979-05-22 Hoffmann-La Roche Inc. Polyene compounds
EP0553814A1 (fr) * 1992-01-29 1993-08-04 Burns Philp Food Inc. Procédé pour améliorer la disponibilité de l'astaxanthine dans Phaffia rhodozyma
WO1999013855A1 (fr) * 1997-09-12 1999-03-25 Sederma S.A. Compositions a usage cosmetique ou dermopharmaceutique contenant une association d'extrait d'algue et d'exopolysaccharide

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1336968C (fr) * 1989-05-16 1995-09-12 J. R. Jocelyn Pare Methode amelioree d'extraction de produits naturels a l'aide d'energie ondulatoire
JP3095241B2 (ja) * 1990-11-15 2000-10-03 カナダ国 揮発性油のマイクロ波抽出法及びそのための装置
JPH08140695A (ja) * 1994-11-25 1996-06-04 Yaegaki Hakko Giken Kk 培養菌体からのアスタキサンチンの分離・精製方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4156090A (en) * 1975-11-30 1979-05-22 Hoffmann-La Roche Inc. Polyene compounds
EP0553814A1 (fr) * 1992-01-29 1993-08-04 Burns Philp Food Inc. Procédé pour améliorer la disponibilité de l'astaxanthine dans Phaffia rhodozyma
WO1999013855A1 (fr) * 1997-09-12 1999-03-25 Sederma S.A. Compositions a usage cosmetique ou dermopharmaceutique contenant une association d'extrait d'algue et d'exopolysaccharide

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIOTECHNOL. TECH., vol. 4, no. 2, 1990, pages 107 - 112 *
CHEMICAL ABSTRACTS, vol. 113, no. 7, 13 August 1990, Columbus, Ohio, US; abstract no. 57352X, SEDMAK J.J. ET AL.: "Extraction and quantitation of astaxanthin from phaffia rhodozyma" page 550; column 1; *
CHEMICAL ABSTRACTS, vol. 129, no. 4, 27 July 1998, Columbus, Ohio, US; abstract no. 38460V, KIYOSHI M. ET AL.: "Extraction of astaxanthin from silver nitrate solution using supercritical fluid extraction" page 309; column 2; XP001157421 *
SOLVENT EXTR. RES. DIV. JPN., vol. 5, 1998, pages 166 - 171 *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005075575A1 (fr) * 2004-02-10 2005-08-18 Nestec S.A. Compositions contenant des isomeres cis d'un compose carotenoide
US7851199B2 (en) 2005-03-18 2010-12-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US9909130B2 (en) 2005-03-18 2018-03-06 Dsm Ip Assets B.V. Production of carotenoids in oleaginous yeast and fungi
US8097761B2 (en) 2006-03-28 2012-01-17 Jx Nippon Oil & Energy Corporation Process for production of carotenoid
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
US9297031B2 (en) 2006-09-28 2016-03-29 Dsm Ip Assets B.V. Production of carotenoids in oleaginous yeast and fungi
EP2192191A4 (fr) * 2007-08-29 2010-09-01 Nippon Oil Corp Procédé de production de caroténoïde
CN101815790B (zh) * 2007-08-29 2013-11-06 新日本石油株式会社 类胡萝卜素的制造方法
WO2009028643A1 (fr) 2007-08-29 2009-03-05 Nippon Oil Corporation Procédé de production de caroténoïde
US10701957B2 (en) 2007-08-29 2020-07-07 Jxtg Nippon Oil & Energy Corporation Method for production of carotenoid
EP2090182A1 (fr) * 2008-01-31 2009-08-19 Yamaha Hatsudoki Kabushiki Kaisha Procédé d'amélioration du goût d'un extrait contenant de l'astaxanthine
EP2402455A4 (fr) * 2009-02-27 2014-10-08 Jx Nippon Oil & Energy Corp Procédé de production d'un caroténoïde
WO2011145113A2 (fr) 2010-05-17 2011-11-24 Dynadis Biotech India Pvt Ltd Procédé de production de cristaux de bêta-carotène et de lycopène de haute pureté à partir de biomasse fongique
US9682932B2 (en) 2010-05-17 2017-06-20 Dynadis Biotech India Private Limited Process for production of high purity beta-carotene and lycopene crystals from fungal biomass
EP2728012A4 (fr) * 2011-06-30 2015-02-25 Kaneka Corp Procédé de production d'une composition de caroténoïdes
US9096508B2 (en) 2011-06-30 2015-08-04 Kaneka Corporation Method for producing carotenoid composition

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Publication number Publication date
KR100388110B1 (ko) 2003-06-18
EP1278725A1 (fr) 2003-01-29
KR20000053886A (ko) 2000-09-05
AU5680701A (en) 2001-11-12
JP2003531888A (ja) 2003-10-28

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