[go: up one dir, main page]

WO2001081533A1 - Dispositif et procede d'electroporation avec reglage automatique de l'amplitude de l'impulsion ou des impulsion(s) electriques en fonction de la mesure pre-impulsion des proprietes electriques de l'echantillon - Google Patents

Dispositif et procede d'electroporation avec reglage automatique de l'amplitude de l'impulsion ou des impulsion(s) electriques en fonction de la mesure pre-impulsion des proprietes electriques de l'echantillon Download PDF

Info

Publication number
WO2001081533A1
WO2001081533A1 PCT/IT2001/000196 IT0100196W WO0181533A1 WO 2001081533 A1 WO2001081533 A1 WO 2001081533A1 IT 0100196 W IT0100196 W IT 0100196W WO 0181533 A1 WO0181533 A1 WO 0181533A1
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
amplitude
impedance
cells
electric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2001/000196
Other languages
English (en)
Inventor
Damijan Miklavcic
Lluis Mir
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Igea SRL
Original Assignee
Igea SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Igea SRL filed Critical Igea SRL
Priority to AU2001258729A priority Critical patent/AU2001258729A1/en
Publication of WO2001081533A1 publication Critical patent/WO2001081533A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Definitions

  • the present invention relates to an electroporation device and method, where amplitude of the electric pulse or pulses is automatically set according to pre-pulse measurement of electric properties of the sample.
  • the molecules may be inorganic substances (e.g. drugs) or organic molecules (cells are known to be inserted, for example, with DNA molecules) .
  • Molecules are introduced using various methods, including: viral vectoring : associating the molecule with a virus, which is then introduced into the cell; chemical vectoring : associating the molecule with a chemical substance for reducing the resistance of the cell membrane and so permitting introduction of the molecule into the cell; and ballistic methods : accelerating the molecule so that it strikes and penetrates the cell membrane.
  • known electroporation devices and methods employ a fixed output voltage (determined, for example, experimentally) , which in certain operating conditions, may be too low, thus preventing introduction of the substances, or too high, thus resulting in irreversible damage to the cell.
  • an electroporation device as described in Claim 1.
  • the present invention also relates to an electroporation method as described in Claim 7.
  • FIG 1 shows, schematically, an electroporation device in accordance with the teachings of the present invention
  • Figure 2 shows a logic operating diagram of the Figure 1 device
  • Figure 3 shows signals produced by the Figure 1 device .
  • Number 1 in Figure 1 indicates as a whole an electroporation device.
  • Device 1 comprises a signal generator, in particular a voltage pulse generator 3 having at least two output electrodes 5; a measuring system 7 connected to output electrodes 5; and an electronic control unit 10 for controlling voltage pulse generator 3 and measuring system 7.
  • Electronic control unit 10 comprises at least one microprocessor 12 cooperating with memory devices, e.g. a RAM memory 14 and EPROM memory 16; and interface devices 18.
  • Pulse generator 3 comprises a digital-analog D/A converter 20, which receives a control signal CNTRL from unit 10 and cooperates at the output with a preamplifying circuit 21; preamplifying circuit 21 has an output connected to the input of a power amplifier 22 in turn having an output communicating with electrodes 5; and electrodes 5, in the example embodiment shown, are each defined by a flat, rectangular metal blade to which the output signal from power amplifier 22 is applied.
  • the electrodes may, of course, differ in shape, structure and size from those shown, e.g. may be designed for use in a laparoscopy process.
  • Measuring system 7 comprises an oscillating circuit 24 for supplying electrodes 5 with an excitation signal,- and a converting circuit 26 supplied by electrodes 5 with a signal in response to the excitation signal.
  • Converting circuit 26 cooperates with a memory 28 (e.g. a RAM memory) which is also connected to a known measuring circuit 30, which also cooperates with converting circuit 26 and with oscillating circuit 24.
  • the measure of impedance may be done in the frequency domain or in the time domain.
  • FIG. 2 shows a block diagram of the operations performed by electroporation device 1 under the control of electronic unit 10.
  • a first block 100 determines the impedance value between electrodes 5. More specifically, the impedance Z( ⁇ ) is measured in known manner by measuring system 7, which may determine one or several of the following parameters for instance the absolute impedance value
  • , the real impedance part Zr, the imaginary part jZo, or angle ⁇ arctg (Zo/Zr) .
  • the device may also measure other electric characteristics such as admittance, resistivity or conductivity including dynamic resistance or dynamic conductivity.
  • block 110 is followed by a block 120, which calculates an objective voltage value Vo on the basis of the measured impedance value Z ( ⁇ ) .
  • the control by. block 110 is normally performed when the device 1 uses a particular multiple electrode (not shown) comprising a number of couples, of electrodes 5.
  • the signal outputted by pulse generator 5 is sequentially supplied, by means of a multiplexer device (not shown) , to the couples of electrodes applied to a substrate containing cells to realize the permeabilization of the substrate.
  • the substrate connected between electrode 5 whose impedance is measured by block 100 may be already permeabilized by the signal produced by another couple of electrode. If no multiple electrode is used and therefore no initial permeabilization caused by a signal outputted by other electrodes is present block 110 may be omitted.
  • Objective voltage Vo may be calculated by means of a table (not shown) in which input impedance values are associated with output objective voltage values.
  • the objective voltage may be calculated by means of a mathematical function (e.g. a linear function not shown) , which supplies an output objective ' voltage value Vo on the basis of input impedance values .
  • the table or function provides for a direct proportional application wherein the objective voltage value Vo is low as impedance is low, and is high as impedance is high.
  • Block 120 is followed by a block 130, which generates a first control signal CNTRLl for pulse generator 3, which, in response, produces a first number of first voltage pulses II which are applied to electrodes 5 ( Figure 3) .
  • First pulses II which are preferably rectangular, have an amplitude corresponding with the objective voltage value Vo calculated previously.
  • a predetermined number of first pulses II are produced, so as to control the amplitude of pulses II.
  • the process may stop.
  • block 130 may be followed by block 140, which generates a further control signal CNTRLu for pulse generator 3, which, in response, produces further voltage pulses If which are applied to electrodes 5 ( Figure 3) .
  • Further pulses If may comprise: . rectangular first further pulses Ifl, the amplitude of which is closed-loop adjustable on the basis of the instantaneous measured impedance value; more specifically, the amplitude of first further pulses Ifl decreases as impedance falls, and increases as impedance rises or remains constant,- . rectangular second further pulses If2 of fixed amplitude; more specifically, pulses If2 have a lower amplitude and a greater time width than first pulses II; and
  • a second operating condition wherein further pulses If have been generated for a predetermined time; and a third operating condition wherein a given instantaneous impedance Z( ⁇ ) measured between electrodes 5 has been reached.
  • tissue portion 35 (shown schematically in Figure 1) containing live cells.
  • the tissue portion may be one forming part of a live being (human, animal or vegetable) or one containing cells removed from a live being (human, animal or vegetable) .
  • Tissue portions are also understood to include cultures of uni- or multicellular organisms.
  • a tissue portion is intended to mean, in general, a substrate of any nature on which live cells or cellular organisms are present .
  • Tissue portion 35 is also applied with a substance (organic or inorganic) 37 to be introduced into the cells .
  • the substance may be applied in a number of different ways, some of which are listed below by way of non-limiting examples: direct application of the substance to the tissue portion, e.g. by applying the tissue portion with a fluid containing the substance; indirect application of the substance, e.g. by introducing the substance into the circulatory system of the tissue portion,- injecting the substance, e.g. using needlelike electrodes 5 , each having an inner conduit containing the substance to be injected into the tissue portion. Needles separate from electrodes 5 may, of course, also be used.
  • the substance introduced may be inorganic or organic, e.g. . a DNA molecule containing one or more regulatory sequences and/or sequences coding for therapeutic genes or genes of interest for biomedical or biotechnological purposes;
  • an oligonucleotide whether natural (phosphodiesters) or modified (inside the backbone of the oligonucleotide, such as phosphosulfates, or at the extremities, by addition of groups to protect the oligonucleotides from digestion by nucleases) - the description of oligonucleotide modification being non-limiting,- .
  • a protein or peptide whether natural or genetically or chemically modified, obtained by natural means or by synthesis, or a molecule mimicking the structure of a protein or peptide, whatever its chemical backbone; . a cytotoxic agent; in particular, of cytotoxic agents, the antibiotic bleomycin or cisplatinum; . a penicillin; .
  • Device 1 is activated to immediately determine the impedance of tissue 35 whose value depends on the electrode geometry and tissue type and the permeability of the cell membranes. If the impedance is too low (block 110), i.e. if the cell membranes are already permeabilized for instance because a multiple electrode has been used and the signal outputted by other electrode has already permeabilized tissue 35, no voltage pulse is generated to prevent damaging tissue 35. Conversely, if the measured impedance value is acceptable - in particular, above the threshold value (block 110) - voltage pulses II are generated, the value of which depends on the previously measured impedance.
  • a low-amplitude pulse is generated if the tissue is already partly permeabilized, in the case of use of multiple electrode, and a higher-amplitude pulse if the tissue is permeabilized poorly.
  • the intensity of the electric field applied is thus related to the actual electric characteristics of the tissue, which in turn depend on the extent to which the cell membranes are permeabilized, thus preventing application of a too high electric field and cell damage in the event the tissue is already permeabilized.
  • First pulses II are thus generated and applied to electrodes 5 to produce an electric field which is directed into the tissue portion to initiate permeabilization of the tissue cell membranes.
  • pulses may be applied to ensure complete permeabilization of tissue 35.
  • pulses If may promote the introduction of high-molecular-weight substances, e.g. DNA.
  • Substance 37 is then introduced into the cells.
  • the knowledge gathered by the Applicant indicates that applying at least one pulse II of an amplitude adjustable according to the impedance measurement provides for achieving a high degree of permeabilization of the cell membranes, while at the same time preventing damage to the cell tissue.
  • the impedance of substrate 35 may also, obviously, be measured by a separate pair of auxiliary electrodes (not shown) close to electrodes 5 and placed in contact with tissue portion 35 to be permeabilized.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electrotherapy Devices (AREA)
  • X-Ray Techniques (AREA)
  • Details Of Television Scanning (AREA)

Abstract

Cette invention concerne une méthode d'électroporation d'un substrat (35) renfermant des cellules, qui consiste à: déterminer (7, 10, 3, 100) l'impédance du substrat (35); calculer une valeur objective (Vo) d'un signal à partir de l'impédance prédéterminée; et fournir au substrat (23), via des électrodes (5), une tension dont la valeur est corrélée avec la valeur objective (Vo) précédemment calculée.
PCT/IT2001/000196 2000-04-21 2001-04-20 Dispositif et procede d'electroporation avec reglage automatique de l'amplitude de l'impulsion ou des impulsion(s) electriques en fonction de la mesure pre-impulsion des proprietes electriques de l'echantillon Ceased WO2001081533A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001258729A AU2001258729A1 (en) 2000-04-21 2001-04-20 Electroporation device and method, where amplitude of the electric pulse or pulses is automatically set according to pre-pulse measurement of electric properties of the sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITTO00A000389 2000-04-21
IT2000TO000389A IT1320186B1 (it) 2000-04-21 2000-04-21 Dispositivo e metodo di elettro-porazione in cui l'ampiezzadell'impulso o degli impulsi viene stabilita in modo automatico in

Publications (1)

Publication Number Publication Date
WO2001081533A1 true WO2001081533A1 (fr) 2001-11-01

Family

ID=11457685

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2001/000196 Ceased WO2001081533A1 (fr) 2000-04-21 2001-04-20 Dispositif et procede d'electroporation avec reglage automatique de l'amplitude de l'impulsion ou des impulsion(s) electriques en fonction de la mesure pre-impulsion des proprietes electriques de l'echantillon

Country Status (3)

Country Link
AU (1) AU2001258729A1 (fr)
IT (1) IT1320186B1 (fr)
WO (1) WO2001081533A1 (fr)

Cited By (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003104448A1 (fr) * 2002-06-07 2003-12-18 Igea S.R.L. Dispositif d'electroporation
FR2873385A1 (fr) * 2004-07-23 2006-01-27 Centre Nat Rech Scient Surveillance et controle d'une electroporation
WO2006112870A1 (fr) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Dispositif et procede pour electroporation lente et administration de molecules dans des cellules et des tissus
DE102007005909A1 (de) 2007-02-01 2008-08-14 Amaxa Ag Verfahren zur Kontrolle eines mit Elektroden versehenen Behältnissen
USRE42016E1 (en) 2001-08-13 2010-12-28 Angiodynamics, Inc. Apparatus and method for the treatment of benign prostatic hyperplasia
USRE42277E1 (en) 2000-08-17 2011-04-05 Angiodynamics, Inc. Apparatus and method for reducing subcutaneous fat deposits, virtual face lift and body sculpturing by electroporation
USRE42835E1 (en) 2000-08-17 2011-10-11 Angiodynamics, Inc. Apparatus and method for reducing subcutaneous fat deposits by electroporation with improved comfort of patients
USRE43009E1 (en) 2000-08-17 2011-12-06 Angiodynamics, Inc. Apparatus and method for reducing subcutaneous fat deposits by electroporation
US8926606B2 (en) 2009-04-09 2015-01-06 Virginia Tech Intellectual Properties, Inc. Integration of very short electric pulses for minimally to noninvasive electroporation
US8992517B2 (en) 2008-04-29 2015-03-31 Virginia Tech Intellectual Properties Inc. Irreversible electroporation to treat aberrant cell masses
US9005189B2 (en) 2003-12-24 2015-04-14 The Regents Of The University Of California Tissue ablation with irreversible electroporation
US9173704B2 (en) 2008-06-20 2015-11-03 Angiodynamics, Inc. Device and method for the ablation of fibrin sheath formation on a venous catheter
US9414881B2 (en) 2012-02-08 2016-08-16 Angiodynamics, Inc. System and method for increasing a target zone for electrical ablation
US9598691B2 (en) 2008-04-29 2017-03-21 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation to create tissue scaffolds
US9681909B2 (en) 2008-06-23 2017-06-20 Angiodynamics, Inc. Treatment devices and methods
US9700368B2 (en) 2010-10-13 2017-07-11 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
US9764145B2 (en) 2009-05-28 2017-09-19 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9867652B2 (en) 2008-04-29 2018-01-16 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
US10010666B2 (en) 2008-03-27 2018-07-03 Angiodynamics, Inc. Balloon catheter method for reducing restenosis via irreversible electroporation
US10117707B2 (en) 2008-04-29 2018-11-06 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US10143512B2 (en) 2009-11-19 2018-12-04 The Regents Of The University Of California Controlled irreversible electroporation
US10154874B2 (en) 2008-04-29 2018-12-18 Virginia Tech Intellectual Properties, Inc. Immunotherapeutic methods using irreversible electroporation
US10166321B2 (en) 2014-01-09 2019-01-01 Angiodynamics, Inc. High-flow port and infusion needle systems
US10238447B2 (en) 2008-04-29 2019-03-26 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
US10272178B2 (en) 2008-04-29 2019-04-30 Virginia Tech Intellectual Properties Inc. Methods for blood-brain barrier disruption using electrical energy
US10292755B2 (en) 2009-04-09 2019-05-21 Virginia Tech Intellectual Properties, Inc. High frequency electroporation for cancer therapy
US10463426B2 (en) 2001-08-13 2019-11-05 Angiodynamics, Inc. Method for treating a tubular anatomical structure
US10470822B2 (en) 2008-04-29 2019-11-12 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US10471254B2 (en) 2014-05-12 2019-11-12 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US10694972B2 (en) 2014-12-15 2020-06-30 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
US10702326B2 (en) 2011-07-15 2020-07-07 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment of stenosis of a tubular body part
US11254926B2 (en) 2008-04-29 2022-02-22 Virginia Tech Intellectual Properties, Inc. Devices and methods for high frequency electroporation
US11272979B2 (en) 2008-04-29 2022-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US11311329B2 (en) 2018-03-13 2022-04-26 Virginia Tech Intellectual Properties, Inc. Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
US11382681B2 (en) 2009-04-09 2022-07-12 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of high frequency electrical pulses for non-thermal ablation
US11453873B2 (en) 2008-04-29 2022-09-27 Virginia Tech Intellectual Properties, Inc. Methods for delivery of biphasic electrical pulses for non-thermal ablation
US11607537B2 (en) 2017-12-05 2023-03-21 Virginia Tech Intellectual Properties, Inc. Method for treating neurological disorders, including tumors, with electroporation
US11638603B2 (en) 2009-04-09 2023-05-02 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US11723710B2 (en) 2016-11-17 2023-08-15 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
US11925405B2 (en) 2018-03-13 2024-03-12 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
US11950835B2 (en) 2019-06-28 2024-04-09 Virginia Tech Intellectual Properties, Inc. Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy
US12114911B2 (en) 2014-08-28 2024-10-15 Angiodynamics, Inc. System and method for ablating a tissue site by electroporation with real-time pulse monitoring
US12201349B2 (en) 2009-04-03 2025-01-21 Angiodynamics, Inc. Congestive obstruction pulmonary disease (COPD)
US12214189B2 (en) 2019-07-24 2025-02-04 Virginia Tech Intellectual Properties, Inc. Fourier analysis spectroscopy for monitoring tissue impedance changes and treatment outcome during electroporation-based-therapies
US12390262B2 (en) 2018-03-13 2025-08-19 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5134070A (en) * 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
WO1996039531A1 (fr) * 1995-06-06 1996-12-12 Massachusetts Institute Of Technology Administration de nucleotides a des organismes par electroporation
WO2000020554A1 (fr) * 1998-10-08 2000-04-13 Astrazeneca Ab Injecteur de cellules microfabrique
WO2001007584A1 (fr) * 1999-07-21 2001-02-01 The Regents Of The University Of California Electroporation controlee et transfert de masse a travers des membranes cellulaires
WO2001007583A1 (fr) * 1999-07-21 2001-02-01 The Regents Of The University Of California Analyse cellulaire/tissulaire par une electroporation regulee

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5134070A (en) * 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
WO1996039531A1 (fr) * 1995-06-06 1996-12-12 Massachusetts Institute Of Technology Administration de nucleotides a des organismes par electroporation
WO2000020554A1 (fr) * 1998-10-08 2000-04-13 Astrazeneca Ab Injecteur de cellules microfabrique
WO2001007584A1 (fr) * 1999-07-21 2001-02-01 The Regents Of The University Of California Electroporation controlee et transfert de masse a travers des membranes cellulaires
WO2001007583A1 (fr) * 1999-07-21 2001-02-01 The Regents Of The University Of California Analyse cellulaire/tissulaire par une electroporation regulee

Cited By (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE42277E1 (en) 2000-08-17 2011-04-05 Angiodynamics, Inc. Apparatus and method for reducing subcutaneous fat deposits, virtual face lift and body sculpturing by electroporation
USRE43009E1 (en) 2000-08-17 2011-12-06 Angiodynamics, Inc. Apparatus and method for reducing subcutaneous fat deposits by electroporation
USRE42835E1 (en) 2000-08-17 2011-10-11 Angiodynamics, Inc. Apparatus and method for reducing subcutaneous fat deposits by electroporation with improved comfort of patients
USRE42016E1 (en) 2001-08-13 2010-12-28 Angiodynamics, Inc. Apparatus and method for the treatment of benign prostatic hyperplasia
US10463426B2 (en) 2001-08-13 2019-11-05 Angiodynamics, Inc. Method for treating a tubular anatomical structure
US7625729B2 (en) 2002-06-07 2009-12-01 Igea S.R.L. Electroporation device
WO2003104448A1 (fr) * 2002-06-07 2003-12-18 Igea S.R.L. Dispositif d'electroporation
US11033321B2 (en) 2003-12-24 2021-06-15 The Regents Of The University Of California Tissue ablation with irreversible electroporation
US9005189B2 (en) 2003-12-24 2015-04-14 The Regents Of The University Of California Tissue ablation with irreversible electroporation
US10117701B2 (en) 2003-12-24 2018-11-06 The Regents Of The University Of California Tissue ablation with irreversible electroporation
WO2006018528A1 (fr) * 2004-07-23 2006-02-23 Centre National De La Recherche Scientifique (Cnrs) Surveillance et controle d’une electroporation
FR2873385A1 (fr) * 2004-07-23 2006-01-27 Centre Nat Rech Scient Surveillance et controle d'une electroporation
WO2006112870A1 (fr) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Dispositif et procede pour electroporation lente et administration de molecules dans des cellules et des tissus
DE102007005909A1 (de) 2007-02-01 2008-08-14 Amaxa Ag Verfahren zur Kontrolle eines mit Elektroden versehenen Behältnissen
US10010666B2 (en) 2008-03-27 2018-07-03 Angiodynamics, Inc. Balloon catheter method for reducing restenosis via irreversible electroporation
US12059197B2 (en) 2008-04-29 2024-08-13 Virginia Tech Intellectual Properties, Inc. Blood-brain barrier disruption using reversible or irreversible electroporation
US10828085B2 (en) 2008-04-29 2020-11-10 Virginia Tech Intellectual Properties, Inc. Immunotherapeutic methods using irreversible electroporation
US11453873B2 (en) 2008-04-29 2022-09-27 Virginia Tech Intellectual Properties, Inc. Methods for delivery of biphasic electrical pulses for non-thermal ablation
US9598691B2 (en) 2008-04-29 2017-03-21 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation to create tissue scaffolds
US9867652B2 (en) 2008-04-29 2018-01-16 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US12173280B2 (en) 2008-04-29 2024-12-24 Virginia Tech Intellectual Properties, Inc. Methods of reducing adverse effects of non-thermal ablation
US12390268B2 (en) 2008-04-29 2025-08-19 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US11655466B2 (en) 2008-04-29 2023-05-23 Virginia Tech Intellectual Properties, Inc. Methods of reducing adverse effects of non-thermal ablation
US10117707B2 (en) 2008-04-29 2018-11-06 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US11607271B2 (en) 2008-04-29 2023-03-21 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US10154874B2 (en) 2008-04-29 2018-12-18 Virginia Tech Intellectual Properties, Inc. Immunotherapeutic methods using irreversible electroporation
US11974800B2 (en) 2008-04-29 2024-05-07 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US10238447B2 (en) 2008-04-29 2019-03-26 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
US10245105B2 (en) 2008-04-29 2019-04-02 Virginia Tech Intellectual Properties, Inc. Electroporation with cooling to treat tissue
US10245098B2 (en) 2008-04-29 2019-04-02 Virginia Tech Intellectual Properties, Inc. Acute blood-brain barrier disruption using electrical energy based therapy
US10272178B2 (en) 2008-04-29 2019-04-30 Virginia Tech Intellectual Properties Inc. Methods for blood-brain barrier disruption using electrical energy
US10286108B2 (en) 2008-04-29 2019-05-14 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation to create tissue scaffolds
US11272979B2 (en) 2008-04-29 2022-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US11254926B2 (en) 2008-04-29 2022-02-22 Virginia Tech Intellectual Properties, Inc. Devices and methods for high frequency electroporation
US8992517B2 (en) 2008-04-29 2015-03-31 Virginia Tech Intellectual Properties Inc. Irreversible electroporation to treat aberrant cell masses
US10470822B2 (en) 2008-04-29 2019-11-12 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US11952568B2 (en) 2008-04-29 2024-04-09 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of biphasic electrical pulses for non-thermal ablation
US10537379B2 (en) 2008-04-29 2020-01-21 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US11890046B2 (en) 2008-04-29 2024-02-06 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
US11737810B2 (en) 2008-04-29 2023-08-29 Virginia Tech Intellectual Properties, Inc. Immunotherapeutic methods using electroporation
US10959772B2 (en) 2008-04-29 2021-03-30 Virginia Tech Intellectual Properties, Inc. Blood-brain barrier disruption using electrical energy
US10828086B2 (en) 2008-04-29 2020-11-10 Virginia Tech Intellectual Properties, Inc. Immunotherapeutic methods using irreversible electroporation
US9173704B2 (en) 2008-06-20 2015-11-03 Angiodynamics, Inc. Device and method for the ablation of fibrin sheath formation on a venous catheter
US9681909B2 (en) 2008-06-23 2017-06-20 Angiodynamics, Inc. Treatment devices and methods
US12201349B2 (en) 2009-04-03 2025-01-21 Angiodynamics, Inc. Congestive obstruction pulmonary disease (COPD)
US8926606B2 (en) 2009-04-09 2015-01-06 Virginia Tech Intellectual Properties, Inc. Integration of very short electric pulses for minimally to noninvasive electroporation
US10448989B2 (en) 2009-04-09 2019-10-22 Virginia Tech Intellectual Properties, Inc. High-frequency electroporation for cancer therapy
US10292755B2 (en) 2009-04-09 2019-05-21 Virginia Tech Intellectual Properties, Inc. High frequency electroporation for cancer therapy
US11382681B2 (en) 2009-04-09 2022-07-12 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of high frequency electrical pulses for non-thermal ablation
US11638603B2 (en) 2009-04-09 2023-05-02 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US9764145B2 (en) 2009-05-28 2017-09-19 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US11707629B2 (en) 2009-05-28 2023-07-25 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
US10143512B2 (en) 2009-11-19 2018-12-04 The Regents Of The University Of California Controlled irreversible electroporation
US9700368B2 (en) 2010-10-13 2017-07-11 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
US12232792B2 (en) 2011-07-15 2025-02-25 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment
US10702326B2 (en) 2011-07-15 2020-07-07 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment of stenosis of a tubular body part
US9414881B2 (en) 2012-02-08 2016-08-16 Angiodynamics, Inc. System and method for increasing a target zone for electrical ablation
US12102376B2 (en) 2012-02-08 2024-10-01 Angiodynamics, Inc. System and method for increasing a target zone for electrical ablation
US11957405B2 (en) 2013-06-13 2024-04-16 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
US10166321B2 (en) 2014-01-09 2019-01-01 Angiodynamics, Inc. High-flow port and infusion needle systems
US11406820B2 (en) 2014-05-12 2022-08-09 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US10471254B2 (en) 2014-05-12 2019-11-12 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US12114911B2 (en) 2014-08-28 2024-10-15 Angiodynamics, Inc. System and method for ablating a tissue site by electroporation with real-time pulse monitoring
US11903690B2 (en) 2014-12-15 2024-02-20 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
US10694972B2 (en) 2014-12-15 2020-06-30 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
US11723710B2 (en) 2016-11-17 2023-08-15 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
US11607537B2 (en) 2017-12-05 2023-03-21 Virginia Tech Intellectual Properties, Inc. Method for treating neurological disorders, including tumors, with electroporation
US11925405B2 (en) 2018-03-13 2024-03-12 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
US12390262B2 (en) 2018-03-13 2025-08-19 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
US11311329B2 (en) 2018-03-13 2022-04-26 Virginia Tech Intellectual Properties, Inc. Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
US11950835B2 (en) 2019-06-28 2024-04-09 Virginia Tech Intellectual Properties, Inc. Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy
US12214189B2 (en) 2019-07-24 2025-02-04 Virginia Tech Intellectual Properties, Inc. Fourier analysis spectroscopy for monitoring tissue impedance changes and treatment outcome during electroporation-based-therapies

Also Published As

Publication number Publication date
ITTO20000389A1 (it) 2001-10-21
IT1320186B1 (it) 2003-11-26
AU2001258729A1 (en) 2001-11-07
ITTO20000389A0 (it) 2000-04-21

Similar Documents

Publication Publication Date Title
WO2001081533A1 (fr) Dispositif et procede d'electroporation avec reglage automatique de l'amplitude de l'impulsion ou des impulsion(s) electriques en fonction de la mesure pre-impulsion des proprietes electriques de l'echantillon
EP1274830B1 (fr) Dispositif d'éléctroporation sous le contrôle continu de la permeabilisation cellulaire
US5869326A (en) Electroporation employing user-configured pulsing scheme
WO2001089455A3 (fr) Systeme et procede permettant d'evaluer l'efficacite d'un dispositif d'administration d'agent pharmaceutique
CA2165714A1 (fr) Moniteur a reaction inductive, avec electrode d'interrogation, pour instrument electrochirurgical
CN115040233B (zh) 不可逆电穿孔组织消融系统及其控制方法
CA2426003A1 (fr) Systeme electrochirurgical
EP1163024A4 (fr) Administration de macromolecules dans des cellules
CA2308881A1 (fr) Systeme et methode d'electrochirurgie
EP1083968A1 (fr) TECHNIQUE D'ELECTROPORATION A FLUX TRAVERSANT POUR THERAPIE GENIQUE $i(EX VIVO)
WO2007111542A8 (fr) Système médical pour surveiller et localiser les fils d'électrodes dans le cœur
JPH1118770A (ja) 細胞電気穿孔法及び回路
CN103604986A (zh) 一种高频电刀的功率检测方法及装置
WO2001080946A1 (fr) Dispositif d'electroporation avec mesure des proprietes electriques
CN109692637B (zh) 一种一体式放电装置及液体放电系统
US7625729B2 (en) Electroporation device
CN211512038U (zh) 一种用于血管内不可逆电穿孔消融的正交电极
KR102548891B1 (ko) 전기천공과 전기분해를 이용한 세포사멸 촉진 장치
Rodamporn Optimal parameters of electroporation for gene and tissue
CN217488824U (zh) 射频消融装置及其生物组织检测电路
JPH02303478A (ja) 遺伝子導入装置
EP4342515A1 (fr) Dispositif d'électroporation amélioré
Novotna et al. Impedance measurement of muscular tissue during electroporation procedure
Hofmann Electroporation system with voltage control feedback for clinical applications
WO2023126855A1 (fr) Sélection de valeurs de paramètres de traitement à l'aide de champs de traitement de tumeur (ttfields)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP