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WO2001077379A3 - Detection de cellules - Google Patents

Detection de cellules Download PDF

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Publication number
WO2001077379A3
WO2001077379A3 PCT/GB2001/001619 GB0101619W WO0177379A3 WO 2001077379 A3 WO2001077379 A3 WO 2001077379A3 GB 0101619 W GB0101619 W GB 0101619W WO 0177379 A3 WO0177379 A3 WO 0177379A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
cells
nucleic acid
detecting
contacting
Prior art date
Application number
PCT/GB2001/001619
Other languages
English (en)
Other versions
WO2001077379A2 (fr
Inventor
Knut Rudi
Original Assignee
Matforsk
Gardner Rebecca
Knut Rudi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matforsk, Gardner Rebecca, Knut Rudi filed Critical Matforsk
Priority to JP2001575233A priority Critical patent/JP4340734B2/ja
Priority to CA002405769A priority patent/CA2405769A1/fr
Priority to EP01919651A priority patent/EP1272664A2/fr
Priority to AU4671201A priority patent/AU4671201A/xx
Priority to AU2001246712A priority patent/AU2001246712C1/en
Priority to US10/257,493 priority patent/US7262009B2/en
Publication of WO2001077379A2 publication Critical patent/WO2001077379A2/fr
Publication of WO2001077379A3 publication Critical patent/WO2001077379A3/fr
Priority to NO20024879A priority patent/NO20024879L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés de détection de cellules dans un échantillon, et des procédés d'obtention d'informations quantitatives concernant des populations de cellules d'un échantillon. Plus spécifiquement, cette invention concerne un procédé servant à distinguer entre cellules vivantes et cellules mortes dans un échantillon, et consistant à : (a) mettre l'échantillon en contact avec une sonde de viabilité modifiant l'acide nucléique des cellules mortes de l'échantillon, et (b) détecter l'acide nucléique des cellules de l'échantillon. Cette invention décrit également un procédé de détection de cellules dans un échantillon, et consistant à: (a) mettre l'échantillon en contact avec une sonde de viabilité marquant l'acide nucléique des cellules mortes de l'échantillon, (b) séparer en fractions marquée et non marquée l'acide nucléique des cellules, et (c) détecter l'acide nucléique dans l'une au moins des deux fractions.
PCT/GB2001/001619 2000-04-10 2001-04-10 Detection de cellules WO2001077379A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2001575233A JP4340734B2 (ja) 2000-04-10 2001-04-10 細胞検出方法
CA002405769A CA2405769A1 (fr) 2000-04-10 2001-04-10 Detection de cellules
EP01919651A EP1272664A2 (fr) 2000-04-10 2001-04-10 Detection de cellules
AU4671201A AU4671201A (en) 2000-04-10 2001-04-10 A method of cell detection
AU2001246712A AU2001246712C1 (en) 2000-04-10 2001-04-10 A method of cell detection
US10/257,493 US7262009B2 (en) 2000-04-10 2001-04-10 Method of cell detection
NO20024879A NO20024879L (no) 2000-04-10 2002-10-09 Fremgangsmåte ved påvisning av celler

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0008787.4A GB0008787D0 (en) 2000-04-10 2000-04-10 A method of cell detection
GB0008787.4 2000-04-10

Publications (2)

Publication Number Publication Date
WO2001077379A2 WO2001077379A2 (fr) 2001-10-18
WO2001077379A3 true WO2001077379A3 (fr) 2002-06-20

Family

ID=9889587

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/001619 WO2001077379A2 (fr) 2000-04-10 2001-04-10 Detection de cellules

Country Status (8)

Country Link
US (1) US7262009B2 (fr)
EP (1) EP1272664A2 (fr)
JP (1) JP4340734B2 (fr)
AU (2) AU4671201A (fr)
CA (1) CA2405769A1 (fr)
GB (1) GB0008787D0 (fr)
NO (1) NO20024879L (fr)
WO (1) WO2001077379A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1769081T3 (en) * 2004-07-01 2016-03-14 Litron Lab Ltd PROCESS FOR COUNTING OF MAMMALS CELL micronuclei WITH A HEAD WEIGHT OF DIFFERENTIAL STAINING OF micronuclei AND chromatin OF DEAD AND DYING CELLS
PL1918385T3 (pl) 2005-07-21 2011-11-30 Morinaga Milk Industry Co Ltd Sposób wykrywania mikroorganizmu oraz zestaw do wykrywania mikroorganizmu
JP4127847B2 (ja) 2006-02-17 2008-07-30 国立大学法人九州大学 微生物検出法及び微生物検出キット
US8198040B2 (en) 2006-02-28 2012-06-12 Montana State University Use of phenanthridium derivatives for distinguishing between live and dead cells
JP4113556B2 (ja) * 2006-12-04 2008-07-09 国立大学法人九州大学 核酸分解剤および核酸の分解方法
CA2666448C (fr) 2007-08-16 2013-09-10 Morinaga Milk Industry Co., Ltd. Procede et trousse de detection de cellules vivantes de microorganismes dans un echantillon
US9394572B2 (en) 2009-07-24 2016-07-19 Morinaga Milk Industry Co., Ltd. Method and kit for detection of microorganism
WO2011043737A1 (fr) * 2009-10-05 2011-04-14 Nanyang Technological University Analyse de viabilité de protozoaires au moyen d'une amplification en chaîne par polymérase (acp)
FR2974117B1 (fr) 2011-04-18 2018-05-11 Societe Des Eaux De Marseille Procede de quantification de bacteries vivantes dans un milieu liquide
US8993259B2 (en) 2012-05-02 2015-03-31 Charles River Laboratories, Inc. Method of viability staining with membrane permeable fluorescent dye and membrane impermeable fluorescence quencher
US10324036B2 (en) 2012-05-02 2019-06-18 Charles River Laboratories, Inc. Porous planar cell capture system
EP2780707B1 (fr) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Procédé de détection de cellules viables dans un échantillon de cellules
DE102020103971B4 (de) * 2020-02-14 2022-02-24 Testo bioAnalytics GmbH Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
EP0881489A1 (fr) * 1995-12-29 1998-12-02 Wako Pure Chemical Industries Ltd Procede de determination du taux de viabilite de cellules

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0517154A1 (fr) * 1991-06-06 1992-12-09 F. Hoffmann-La Roche Ag Procédé de détection de giardia
US5525471A (en) * 1994-10-12 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enzymatic degrading subtraction hybridization
US5780278A (en) * 1996-05-20 1998-07-14 Vanderbilt University IceA gene and related methods
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
EP0881489A1 (fr) * 1995-12-29 1998-12-02 Wako Pure Chemical Industries Ltd Procede de determination du taux de viabilite de cellules

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CIMINO G D ET AL: "POST-PCR STERILIZATION: A METHOD TO CONTROL CARRYOVER CONTAMINATIONFOR THE POLYMERASE CHAIN REACTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 1, 11 January 1991 (1991-01-11), pages 99 - 107, XP000471620, ISSN: 0305-1048 *
LANGSRUD S AND SUNDHEIM G: "Flow cytometry for rapid assessment of viability after exposure to a quaternary ammonium compound", JOURNAL OF APPLIED BACTERIOLOGY, vol. 81, 1996, pages 411 - 418, XP008002041 *
O'BRIEN M AND BOLTON W E: "Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry", CYTOMETRY, vol. 19, 1995, pages 243 - 255, XP001064602 *
ORMEROD M G ET AL.: "Quantification of apoptosis and necrosis by flow cytometry", ACTA ONCOLOGICA, vol. 32, no. 4, 1993, pages 417 - 424, XP008002042 *
RIEDY M C ET AL: "USE OF A PHOTOLABELING TECHNIQUE TO IDENTIFY NONVIABLE CELLS IN FIXED HOMOLOGOUS OR HETEROLOGOUS CELL POPULATIONS", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 12, no. 2, 1991, pages 133 - 139, XP001037495, ISSN: 0196-4763 *
ROTH B L ET AL: "BACTERIAL VIABILITY AND ANTIBIOTIC SUSCEPTIBILITY TESTING WITH SYTOX GREEN NUCLEIC ACID STAIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 63, no. 6, 1997, pages 2421 - 2431, XP000929110, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
WO2001077379A2 (fr) 2001-10-18
AU2001246712B2 (en) 2006-10-19
US7262009B2 (en) 2007-08-28
NO20024879D0 (no) 2002-10-09
CA2405769A1 (fr) 2001-10-18
GB0008787D0 (en) 2000-05-31
JP4340734B2 (ja) 2009-10-07
NO20024879L (no) 2002-12-10
AU4671201A (en) 2001-10-23
JP2003530118A (ja) 2003-10-14
EP1272664A2 (fr) 2003-01-08
US20030203374A1 (en) 2003-10-30
AU2001246712C1 (en) 2007-08-02

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