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WO2001072813A1 - Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide - Google Patents

Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide Download PDF

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Publication number
WO2001072813A1
WO2001072813A1 PCT/CN2001/000480 CN0100480W WO0172813A1 WO 2001072813 A1 WO2001072813 A1 WO 2001072813A1 CN 0100480 W CN0100480 W CN 0100480W WO 0172813 A1 WO0172813 A1 WO 0172813A1
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
polynucleotide
coupling factor
human mitochondrial
mitochondrial atpase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2001/000480
Other languages
English (en)
Chinese (zh)
Inventor
Yumin Mao
Yi Xie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Biowindow Gene Development Inc
Original Assignee
Shanghai Biowindow Gene Development Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Biowindow Gene Development Inc filed Critical Shanghai Biowindow Gene Development Inc
Priority to AU63718/01A priority Critical patent/AU6371801A/en
Publication of WO2001072813A1 publication Critical patent/WO2001072813A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals

Definitions

  • the present invention provides a new polypeptide, human mitochondrial ATPase coupling factor 6-14, which is basically composed of the amino acid sequence shown in SEQ ID NO: 2.
  • the polypeptide of the present invention may be a recombinant polypeptide, a natural polypeptide, or a synthetic polypeptide, and preferably a recombinant polypeptide.
  • the polypeptides of the present invention can be naturally purified products or chemically synthesized products, or can be produced from prokaryotic or eukaryotic hosts (eg, bacteria, yeast, higher plants, insects, and mammalian cells) using recombinant techniques.
  • the invention provides an isolated nucleic acid (polynucleotide), which basically consists of a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2.
  • the polynucleotide sequence of the present invention includes the nucleotide sequence of SEQ ID NO: 1.
  • the polynucleotide of the present invention is found from a cDNA library of human fetal brain tissue. It contains a polynucleotide sequence that is 897 bases in length and its open reading frame 157-552 encodes 1 31 amino acids.
  • Methods known to those skilled in the art can be used to construct expression vectors containing DNA sequences encoding human mitochondrial ATPase coupling factor 6-14 and appropriate transcriptional / translational regulatory elements. These methods include in vitro recombinant DM technology, DNA synthesis technology, in vivo recombination technology, etc. (Sambroook, et'al. Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory. New York, 1989).
  • the DNA sequence can be operably linked to an appropriate promoter in the expression vector to guide mRNA synthesis. Representative examples of these promoters are: the lac or trp promoter of E.
  • Dye terminate cycle reaction ion sequencing kit Perkin-Elraer
  • ABI 377 automatic sequencer Perkin-Elmer
  • the determined cDNA sequence was compared with the existing public DNA sequence database (Genebank), and it was found that the cDNA sequence of one of the clones 0065f05 was new DNA.
  • a series of primers were synthesized to determine the inserted cDNA fragments of the clone in both directions.
  • Primerl 5, — GGATGTGTAGCTTTGGAAATCTGT —3, (SEQ ID NO: 3)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un nouveau polypeptide, un facteur humain 6-14 de couplage de l'ATPase mitochondriale, et un polynucléotide codant pour ce polypeptide ainsi qu'un procédé d'obtention de ce polypeptide par des techniques recombinantes d'ADN. L'invention concerne en outre les applications de ce polypeptide dans le traitement de maladies, notamment des tumeurs malignes, de l'hémopathie, de l'infection par VIH, de maladies immunitaires et de diverses inflammations. L'invention concerne aussi l'antagoniste agissant contre le polypeptide et son action thérapeutique ainsi que les applications de ce polynucléotide codant pour le facteur humain 6-14 de couplage de l'ATPase mitochondriale.
PCT/CN2001/000480 2000-03-28 2001-03-26 Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide Ceased WO2001072813A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU63718/01A AU6371801A (en) 2000-03-28 2001-03-26 A novel polypeptide-human mitochondrial atpase coupling factor 6-14 and a polynucleotide sequence encoding the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN 00115195 CN1315396A (zh) 2000-03-28 2000-03-28 一种新的多肽——人线粒体atp酶耦合因子6－14和编码这种多肽的多核苷酸
CN00115195.9 2000-03-28

Publications (1)

Publication Number Publication Date
WO2001072813A1 true WO2001072813A1 (fr) 2001-10-04

Family

ID=4584663

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2001/000480 Ceased WO2001072813A1 (fr) 2000-03-28 2001-03-26 Nouveau polypeptide, facteur humain 6-14 de couplage de l'atpase mitochondriale, et polynucleotide codant pour ce polypeptide

Country Status (3)

Country Link
CN (1) CN1315396A (fr)
AU (1) AU6371801A (fr)
WO (1) WO2001072813A1 (fr)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIOCHIM. BIOPHYS. ACTA, vol. 1089, no. 3, 1991, pages 393 - 395 *
GENE, vol. 116, no. 2, 1992, pages 291 - 292 *
GENE, vol. 97, no. 2, 1991, pages 307 - 310 *
J. BIOL. CHEM., vol. 262, no. 7, 1987, pages 3007 - 3010 *

Also Published As

Publication number Publication date
CN1315396A (zh) 2001-10-03
AU6371801A (en) 2001-10-08

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